Home | Community | Message Board


Cannabis Seeds - Original Sensible Seeds
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Getting Started

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Amazon ½ Pint Jars, Agar, Parafilm

Jump to first unread post. Pages: 1 | 2  [ show all ]
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Suggestions on syringe to agar
    #25896119 - 03/25/19 05:25 PM (1 year, 11 months ago)

Hello! Got some syringes before I knew of this site and all this awesome info. From reddit users no less... 2 from one user and 1 from another.. lol whoops. I'm trying to get going but can't help but over think things. I was wondering if somebody would enlighten me on a few things as I start my journey.

Also trying to get my post count a little up there and whilst being on a phone. Bare with me lol

I've exhausted and dumbed down my search words as much as possible but kept getting sidetracked with other conflicting questions and other responses that popped into mind. So I'm bucking up and just gonna ask here instead of hijacking a random thread(s).

(Following BOD's easy af TEKs)

1.) What is the best way to inoc. an agar dish with a syringe?

So I've read that you can use foil to place a squirt of LC to it then use the inoculation loop technique to get some decent growth goin.

I did this to 20 plates and only got bacteria growth lol FML. Plates were "sterile" said so on package which was bought through Amazon.
I'm fairly confident in my cross contamination techniques.
I flamed everything that I could between swabs. 90% iso before flamed.

Perhaps my foil was tainted?
Bad syringe?
Very possibly could be my technique overall.

I was thinking of torching a scalpel, cutting out a section and using a drop or less in created hole. I was thinking of just dropping it right in the middle of the agar itself. However when it comes to wrapping it up after inoculation wouldn't the drop roll around and create a giant mess?


(I'm trying to get a spore print from my own yield since I invested in these syringes. Might as well try to get somethin out of it. Experience the highs and lows of things.)

Thanks for reading and the help. Also great message board! I've read more these past few weeks than I have in awhile lol thanks for keeping my brain and eyes in check.


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 1 year, 4 months
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25896147 - 03/25/19 05:36 PM (1 year, 11 months ago)

Hi! If you got syringes on Reddit you have no clue what technique was used to make them. It's totally possible that you got bunk syringes. My first buy was from there too, half were horrible shit that grew nothing but bacteria.

With that said! Another possibility is the agar plates you bought, as it sounds like you bought pre-made agar. First, what type of agar did you buy? If it's the wrong medium mycelium isn't as likely to grow.

With that said, try to avoid those plates. You likely could have made and poured about 100+ plates with the cost for those 20. Follow teks to also make your own agar to know it's sterile.

My honest guess is bad syringes tho. Sticking to spore prints scraped to agar is my method when needed, you can see the spores then, a bit easier, less chances for contam to grab hold. Out of 20 you should have seen at least 1 plate take hold based on what you did.


Edited by ShaperDreaming (03/25/19 05:38 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: ShaperDreaming]
    #25896166 - 03/25/19 05:48 PM (1 year, 11 months ago)

Hey thanks for reading!
Yeah so I figured as much about the luck of the draw from reddit. *Fingers crossed still*

I bought just sterile plates then made my own agar.
I pretty much followed BOD's stuff through and through to the best of my ability :smile: I'm sure I could have done things a bit tidier but it's a learning experience!


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleFeasoghorm


Registered: 10/24/18
Posts: 2,996
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25896289 - 03/25/19 06:50 PM (1 year, 11 months ago)

Rad. Yeah, i jst sanitize my sab and basically jst squeeze a drop on each plate. You get the technique down quick. The plates arent even exposed in the sab for 2 seconds/ea. I'll still get like one contaminated plate every 25 or so.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: Feasoghorm]
    #25897676 - 03/26/19 11:40 AM (1 year, 11 months ago)

Awesome thanks. My first plate I did, squirted out so much!! Ah! I did 13 plates between two syringes last night so I'm hoping for a good outcome from one of them at the very least.

A little more difficult than I thought it was going to be šŸ˜…


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflinebadspongeS
 User Gallery
Registered: 12/20/18
Posts: 869
Loc: SoCal
Last seen: 28 days, 18 hours
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25897937 - 03/26/19 02:02 PM (1 year, 11 months ago)

I made all the exact same mistakes and re-adjustments you did LOL.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offline5deadlyvenoms
just reading

Registered: 01/16/19
Posts: 74
Last seen: 20 hours, 14 minutes
Re: Suggestions on syringe to agar [Re: badsponge]
    #25898012 - 03/26/19 02:52 PM (1 year, 11 months ago)

I use an inoculation loop when going MS syringe to agar. It's been working well so far as opposed to one drop in there center of the agar.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: 5deadlyvenoms]
    #25908735 - 04/01/19 01:08 AM (1 year, 10 months ago)

I actually did that the first run and all 20 plates ended up being contaminated with bacteria.. yik.

I even red hot the loop but maybe the foil I used the drop on wasn't clean from improperly handling it something..

So far it has been 6 days. No bacteria present yet! Better start than the last run thus far *fingers crossed*


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineJxshy12
Stranger

Registered: 03/27/19
Posts: 114
Last seen: 2 months, 18 days
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25909383 - 04/01/19 01:15 PM (1 year, 10 months ago)

Check out Sporeworks for syringes bro, Iā€™m just now getting into all this but they are amazing


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: Jxshy12]
    #25922873 - 04/08/19 12:57 PM (1 year, 10 months ago)

I'll look into it. Thanks :smile: I'm probably going to invest in prints from here on out but try to use what I got in the meantime. Thanks for the suggestion


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineEazybreezy02
Female User Gallery

Registered: 02/07/19
Posts: 23
Last seen: 1 month, 17 days
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25922934 - 04/08/19 01:26 PM (1 year, 10 months ago)

Use 70% ISO - it takes less time to evaporate hence killing more unwanted bacteria on the surfaces of things. I may have missed it in your thread but did you use a still-air box? Did you PC the agar and foil first? You should see some action within 6 days already. Unfortunately there is a strong possibility that the syringes were already contaminated if you followed the teks and was careful about sterilization.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: Eazybreezy02]
    #25923004 - 04/08/19 01:53 PM (1 year, 10 months ago)

Yup. I used 70. I bathe in when I first start the project. I used a sab. Agar was pc'd with 10 minutes of steam before placing weight to reach psi.
Agar work is good.
Maybe putting the plates in the sab straight from the bag is contaminating the edges for some... (Would I wipe down every single dish? I try to stack them straight out of the bag which I find is difficult to do)

Foil was not sterile to which I knew but didn't know it was going to do what it did lol. That is the reason the first sleeve of plates went to shit. I stopped using foil.

The second batch I did was with a different technique and that was to do just a drop of Ms  straight into agar.  lol I was sure this was going to be a shit show also. And it was.
It lead up to growth around edges and some bacteria. Some plates didn't even do anything
(Still watching to see what happens to all the plates)

Third batch I did recently with a different method of innoc. so we shall see something if anything I hope. Ms to flame sterile loop and T-streaked. (I made sure to cool each flamed tool. Squirted for syringe/agar pressed for loop)

Trial and error #newb


--------------------
:computertrip:


Edited by RhYzo (04/08/19 02:00 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinepnoobensis
Stranger
 User Gallery

Registered: 01/28/18
Posts: 19
Last seen: 5 months, 21 days
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25923670 - 04/08/19 08:14 PM (1 year, 10 months ago)

Like other posters have said, with such widespread contamination you can probably blame the syringes. But good luck on the next round.

I second the advice to a) work from prints; and b) make your own agar. I PC for 30-35 minutes but realize that half the time is probably fine. 

Are you wrapping your plates with parafilm or at least cling wrap?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleHobbit GDF
Deadhead
Male User Gallery


Registered: 02/14/19
Posts: 1,651
Loc: Terrapin station Flag
Re: Suggestions on syringe to agar [Re: pnoobensis]
    #25923756 - 04/08/19 08:57 PM (1 year, 10 months ago)

I have my agar in lil glass bell jars with a hole and micropore tape on top. 2 layers. Do I still need to wrap in suran wrap?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblenube424
Male
Registered: 12/03/17
Posts: 6,063
Re: Suggestions on syringe to agar [Re: Hobbit GDF]
    #25924312 - 04/09/19 07:44 AM (1 year, 10 months ago)

No. That s basically a "no pour".. no a gram wrap needed.

If they start to dry out u might need 2 layers of tape. That's why I like poly.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: pnoobensis]
    #25924683 - 04/09/19 11:44 AM (1 year, 10 months ago)

Welp my next round contaminated so I got 2 other syringes to try before I give in and purchase a print.

I make my own agar :smile: and yes I wrap 2+ times with cling wrap after I innoc all the plates


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinepnoobensis
Stranger
 User Gallery

Registered: 01/28/18
Posts: 19
Last seen: 5 months, 21 days
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25924931 - 04/09/19 02:25 PM (1 year, 10 months ago)

I vote don't spend money; find a friend or else write to the Free Spore Ring Exchange.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblenube424
Male
Registered: 12/03/17
Posts: 6,063
Re: Suggestions on syringe to agar [Re: pnoobensis]
    #25925014 - 04/09/19 03:06 PM (1 year, 10 months ago)

Well 12 more posts and like 20 days u can access the marketplace and get a print from a giveaway.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: nube424]
    #25927372 - 04/10/19 07:42 PM (1 year, 10 months ago)

I don't mind spending on something that'll get me started to get where I need and want to be ya know? But I definitely wanna be able to trade as well but getting there is trialing lol

I'm excited to see what this marketplace is all about! It's like a secret society or something


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 1 year, 4 months
Re: Suggestions on syringe to agar [Re: RhYzo]
    #25928382 - 04/11/19 11:30 AM (1 year, 10 months ago)

Quote:

Sigh4.fg said:
I'm excited to see what this marketplace is all about! It's like a secret society or something




It's far less exciting than you may think :wink: When I first got access I was like  :partyanimals:  then I realized that mush cult provides well enough.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineRhYzo
D1g1t@l Dw3ll3r
I'm a teapot User Gallery


Registered: 01/30/19
Posts: 283
Loc: Wormhole
Last seen: 5 days, 23 hours
Re: Suggestions on syringe to agar [Re: ShaperDreaming]
    #25967864 - 05/02/19 11:20 AM (1 year, 9 months ago)

I'm a few posts shy but interested none the less!


--------------------
:computertrip:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Amazon ½ Pint Jars, Agar, Parafilm

Mushrooms, Mycology and Psychedelics >> Getting Started

Similar ThreadsPosterViewsRepliesLast post
* Is it time to dump my tubs? magu 253 8 05/02/19 11:18 AM
by SFS96

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Ythan, geokills, Asante, Shroomism, RogerRabbit, mushboy, fahtster, wildernessjunkie, Ballerium, cronicr, bodhisatta
815 topic views. 5 members, 9 guests and 15 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Kratom Eye
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.06 seconds spending 0.013 seconds on 13 queries.