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Offlineteaker
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Registered: 08/19/02
Posts: 100
Last seen: 2 years, 26 days
First time agar feedback
    #25888900 - 03/21/19 07:24 PM (5 years, 9 months ago)

Hola amigos!

I am trying my hand at agar for the first time and am looking for some feedback.  All of these are first transfer plates.  The GT are from three different plates noc'd up from a GT print (GT A, B and C). The "skinny" and "fat" plates were taken from two plates started with PE clones - one tall and skinny fruit and one shorter and fatter clone.  Notably, the GT are mostly tomentose while the PE are rhizo.

Do you see anything out of the ordinary at this point?  Any suggestions going forward? Should I take transfers now or let the mycelium grow a bit more?
Any areas I definitely should or should not transfer from?  Should I transfer one more time and then A2G it (assuming everything stays clean), or do you recommend additional transfers?

Thanks in advance!

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InvisibleSunnyDayze
Finder of shrubberies
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Registered: 12/30/17
Posts: 2,230
Loc: Canada Flag
Re: First time agar feedback [Re: teaker]
    #25889124 - 03/21/19 09:10 PM (5 years, 9 months ago)

They are looking great.  I would give them all one more transfer (some might need more) from the leading edge in the areas that are nice and uniform looking.  Stay away from the whispy uneven spots.  Some of the GT’s look like they could go a couple more days before transferring, gives you a better idea of what looks nicest.


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Invisiblejuniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
Re: First time agar feedback [Re: SunnyDayze]
    #25889146 - 03/21/19 09:26 PM (5 years, 9 months ago)

:whatshesaid:

Look pretty good for the most part. A couple more transfers are not a bad idea. Try to transfer from the most "organized" sections of the plate, where you see strong, even growth.


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To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas

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OfflineSFS96
AstroMan
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Registered: 12/09/18
Posts: 2,146
Loc: Valleys Of Neptune
Last seen: 7 months, 29 days
Re: First time agar feedback [Re: juniperus]
    #25889282 - 03/21/19 10:49 PM (5 years, 9 months ago)

Looking good :thumbup: The other guys covered it well. I normally make 3 transfers before I go to grain unless it needs more. Just take from your best growth each time, those are looking really good for your first agar transfer. Nice job:rockon:


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How I make and preserve tea


Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood

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InvisibleXnMe
Somth'n of a Somth'n
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Registered: 06/23/16
Posts: 1,284
Re: First time agar feedback [Re: SFS96]
    #25939015 - 04/16/19 10:25 PM (5 years, 8 months ago)

How'd them GT plates turn out??!


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