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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
#25888054 - 03/21/19 12:52 PM (5 years, 1 month ago) |
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One culture... cubensis
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Mr. Funguy
Quasi Frodo


Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 4 years, 6 months
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47] 1
#25888055 - 03/21/19 12:53 PM (5 years, 1 month ago) |
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Yep, still striving for that perfect culture, but I dont have nearly the proper equipment. Instead just doing what I can with what I have. Working on PE6/APE/NAPE next, dont know how itll perform with anything I've been testing. Been researching it tremendously, we'll see what happens. Until then, here's an upskirt...
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
#25888056 - 03/21/19 12:53 PM (5 years, 1 month ago) |
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A monoculture and isolate are pretty much the same thing some people call them monocultures and others isolates...
But yes an isolated can have multiple strains
Dude you're wrong ... so then an ms plate would be a mono culture??
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
#25888062 - 03/21/19 12:54 PM (5 years, 1 month ago) |
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Technically... not the application in our cases... ms culture, clone monoculture, isolated strains
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Boogieman47
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Registered: 03/05/16
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Re: Post Your Cultivation Picture of The Day [Re: Caps McGee]
#25888078 - 03/21/19 12:59 PM (5 years, 1 month ago) |
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"Technically" you can find multiple strains in an isolate look it up
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
#25888084 - 03/21/19 01:01 PM (5 years, 1 month ago) |
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What's isolated exactly?
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Boogieman47
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Registered: 03/05/16
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Re: Post Your Cultivation Picture of The Day [Re: Caps McGee]
#25888089 - 03/21/19 01:04 PM (5 years, 1 month ago) |
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You're isolating the strains down isolating genetics .... too be honest I cant even remember if you can truly have a single strain
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Boogieman47
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Registered: 03/05/16
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
#25888095 - 03/21/19 01:06 PM (5 years, 1 month ago) |
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Find peoples pictures of their isolates and look at them then tell me if you dont see different traits in the mushrooms... if it was a single strain they would all be the same height weight everything .. I have never seen that
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FishLevelMidnight
Aquaman



Registered: 09/01/17
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Re: Post Your Cultivation Picture of The Day [Re: Caps McGee] 1
#25888109 - 03/21/19 01:08 PM (5 years, 1 month ago) |
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Quote:
Caps McGee said: Technically... not the application in our cases... ms culture, clone monoculture, isolated strains
As far as I am aware a monoculture in mycology is an isolate. What I am not clear on is if Boogie is right where an isolate can be multiple strains. Maybe it is like big C and little c communism- an idea and a practical version- where an isolate can be multiple strains because it is very difficult to get 1 genetic isolate.
There are scientific papers with isolates of the same species for a lot of different mycology applications so I would assume that a true monoculure/isolate is a plate/culture of 1 species that has 1 set of genes (if haploid, 2 if diploid etc) and would not contain multiple strains. 
Monoculture in a broader biological context means a single species in a given area (most farmers use monoculture of a single plant species) but as this is a mycology site I would personally avoid mixing up people with this terminology caps.
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FishLevelMidnight
Aquaman



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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47] 1
#25888118 - 03/21/19 01:11 PM (5 years, 1 month ago) |
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Quote:
Boogieman47 said: Find peoples pictures of their isolates and look at them then tell me if you dont see different traits in the mushrooms... if it was a single strain they would all be the same height weight everything .. I have never seen that
This is absolutely incorrect.
Fungi have advanced enough genetics to have growth variability based off environmental influences.
Even a genetic clone can have differences in those phenotypes that are affected by the environment. Environment can be microeenvironment also (so even though a "tub" is one environment a particular cell can have a different microenvironment based off distribution of spawn vs bulk, presence of bacteria beneficial or not, etc)
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47] 1
#25888125 - 03/21/19 01:12 PM (5 years, 1 month ago) |
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Idk man... but not worth going on about... from my understanding, an MS culture contains hudreds(even thousands)of strains and is technically a monoculture if it's clean, a monoculture (as we use the term) is still multiple strains, but performing together as one (typically a clone culture), and an isolate is a single set of genetics derived from isolation of a given monoculture... seems like it'd be nearly impossible to achieve without some serious equipment though... I like clone monocultures personally, and of the limited testing I've run, they typically outperform cultures with further isolation efforts (safely worded I suppose)
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Liloldme has been pumping out isolate/monoculture pics consistently for awhile, I think he got bored posting because they all look identical.
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Boogieman47
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Registered: 03/05/16
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Ok then I take that back... in my mind I do understand about the phenotypes and characteristics of genetics but in the same sense if it was a single strain wouldnt it act more consistent then not? I mean I feel you on environmental and all that but still a single strain is a single strain but in that I may be confused ...
Yeah lil has some bad ass genetics but I'm just saying the argument is over the fact that we can still have multiple strains in a isolate...
Fk what up man hope you're all good I still feel bad about not being able to come see ya
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
#25888168 - 03/21/19 01:30 PM (5 years, 1 month ago) |
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Doing well, just talking about some bs with strangers who have too much free time like me.
I think the concensus is monoculture == isolate. If you want to consider an isolate any ms that has had an isolation done to be an isolate sure isolates can have multiple strains. I just don't think that's what anyone thinks of when talking about isolate.
I think maybe that's what's confusing people about what you're saying.
Sad not to post any pics right now but I feel something coming in the future...
-------------------- But if you're in a hurry, and really got to go If you're in a hurry, might have to find out slow That it's one thing to try and another to fly You get there quicker just a step at a time It's one thing to bark, another to bite The show ain't over till you pack up at night
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Mr. Funguy
Quasi Frodo


Registered: 01/19/19
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Re: Post Your Cultivation Picture of The Day [Re: filthyknees]
#25888227 - 03/21/19 01:58 PM (5 years, 1 month ago) |
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Jakeoncid419
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Re: Post Your Cultivation Picture of The Day [Re: filthyknees]
#25888233 - 03/21/19 02:01 PM (5 years, 1 month ago) |
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a mono culture is a single homogeneous culture without diversity. isolates are hunted traits you have bred together in hope of them appearing of fruits. getting these traits to consistently appear is what is referred to as "stabilizing" I use mono cultures to make iso's (however until I got my microscope I more than likely was just breeding iso's) i am however self taught so i cant say with 100% confidence that this is the accepted vernaculars but in my mind it stands to reason, and is supported by defintion
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
Edited by Jakeoncid419 (03/21/19 02:33 PM)
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
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Re: Post Your Cultivation Picture of The Day [Re: filthyknees] 1
#25888245 - 03/21/19 02:10 PM (5 years, 1 month ago) |
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Lol... monoculture-one culture (literal translation)... isolate- isolated (1 set)genetics (logical deduction)... seems straight forward to me I've put it 3 different ways and you still twisted it up lmao
Try again here... what I'm saying is:
A clean MS culture IS a monoculture, NOT an isolate: as it contains only one culture... this is technically speaking, and not generally the terminology used in mycology(as we call it an MS culture to distinguish the low-level of isolation applied)
A monoculture (generally speaking-in mycology) is a term applied to a culture containing multiple strains performing together as one; like in a clone culture
An isolate- a single set of genetics... one (isolated (As the term implies)) strain
How is this NOT the case? Lmao, is what it is, but damn
Well put jake
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Jakeoncid419
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Re: Post Your Cultivation Picture of The Day [Re: Caps McGee] 1
#25888253 - 03/21/19 02:16 PM (5 years, 1 month ago) |
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Quote:
Caps McGee said: Lol... monoculture-one culture (literal translation)... isolate- isolated (1 set)genetics (logical deduction)... seems straight forward to me I've put it 3 different ways and you still twisted it up lmao
Try again here... what I'm saying is:
A clean MS culture IS a monoculture, NOT an isolate: as it contains only one culture... this is technically speaking, and not generally the terminology used in mycology(as we call it an MS culture to distinguish the low-level of isolation applied)
A monoculture (generally speaking-in mycology) is a term applied to a culture containing multiple strains performing together as one; like in a clone culture
An isolate- a single set of genetics... one (isolated (As the term implies)) strain
How is this NOT the case? Lmao, is what it is, but damn
Well put jake
I would not refer to ms as mono culture tho not in anysense. its the exact opposite. I belive those mycologists that you heard used it for a multispore were mistaken. a multispore has only one stain of mushroom but many random substrains (some not even fruiters) . a mono culture has one substrain, a iso has an array of selected ideal substrains
I supposed you MS is comprised of lots of mono cultures. if you mean mono culture as in ther is only one stain/species on the plate then yes I suppose you could say you isolated the strain but it still would not be an isolate, it would be a monostrain
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
Edited by Jakeoncid419 (03/21/19 02:30 PM)
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hamonz
Junglist



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Re: Post Your Cultivation Picture of The Day [Re: Caps McGee] 3
#25888259 - 03/21/19 02:19 PM (5 years, 1 month ago) |
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Its been a while since I've posted, but I have not been lazy

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Jakeoncid419
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Re: Post Your Cultivation Picture of The Day [Re: hamonz] 1
#25888294 - 03/21/19 02:39 PM (5 years, 1 month ago) |
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speaking of ms here are some pan MS tays I running in my pursuit of good traits for iso's thes are pan aus and my mystery pan just starting to come up.
-------------------- Natural omt/detox online pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available JOC PAN TEK CORDYCEPS MILITARIS EXOTICS [
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