Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Mono Tub Substrate   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Myyco.com APE Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: < First | < Back | 4056 | 4057 | 4058 | 4059 | 4060 | 4061 | 4062 | 4063 | 4064 | 4065 | 4066 | 4067 | 4068 | 4069 | 4070 | 4071 | 4072 | 4073 | 4074 | 4075 | 4076 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
    #25888054 - 03/21/19 12:52 PM (5 years, 1 month ago)

One culture... cubensis

Extras: Filter Print Post Top
OfflineMr. Funguy
Quasi Frodo
Male User Gallery

Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 4 years, 6 months
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47] * 1
    #25888055 - 03/21/19 12:53 PM (5 years, 1 month ago)

Yep, still striving for that perfect culture, but I dont have nearly the proper equipment. Instead just doing what I can with what I have. Working on PE6/APE/NAPE next, dont know how itll perform with anything I've been testing. Been researching it tremendously, we'll see what happens. Until then, here's an upskirt...


--------------------

Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
    #25888056 - 03/21/19 12:53 PM (5 years, 1 month ago)

A monoculture and isolate are pretty much the same thing some people call them monocultures  and others isolates... 


But yes an isolated can have multiple strains

Dude you're wrong ... so then an ms plate would be a mono culture??


--------------------

Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
    #25888062 - 03/21/19 12:54 PM (5 years, 1 month ago)

Technically... not the application in our cases... ms culture, clone monoculture,  isolated strains

Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Post Your Cultivation Picture of The Day [Re: Caps McGee]
    #25888078 - 03/21/19 12:59 PM (5 years, 1 month ago)

"Technically" you can find multiple strains in an isolate look it up


--------------------

Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
    #25888084 - 03/21/19 01:01 PM (5 years, 1 month ago)

What's isolated exactly?

Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Post Your Cultivation Picture of The Day [Re: Caps McGee]
    #25888089 - 03/21/19 01:04 PM (5 years, 1 month ago)

You're isolating the strains down isolating genetics .... too be honest I cant even remember if you can truly have a single strain


--------------------

Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
    #25888095 - 03/21/19 01:06 PM (5 years, 1 month ago)

Find peoples pictures of their isolates and look at them then tell me if you dont see different traits in the mushrooms... if it was a single strain they would all be the same height weight everything ..  I have never seen that


--------------------

Extras: Filter Print Post Top
OfflineFishLevelMidnight
Aquaman
Male User Gallery


Registered: 09/01/17
Posts: 2,328
Last seen: 19 days, 12 hours
Re: Post Your Cultivation Picture of The Day [Re: Caps McGee] * 1
    #25888109 - 03/21/19 01:08 PM (5 years, 1 month ago)

Quote:

Caps McGee said:
Technically... not the application in our cases... ms culture, clone monoculture,  isolated strains




As far as I am aware a monoculture in mycology is an isolate. What I am not clear on is if Boogie is right where an isolate can be multiple strains. Maybe it is like big C and little c communism- an idea and a practical version- where an isolate can be multiple strains because it is very difficult to get 1 genetic isolate.

There are scientific papers with isolates of the same species for a lot of different mycology applications so I would assume that a true monoculure/isolate is a plate/culture of 1 species that has 1 set of genes (if haploid, 2 if diploid etc) and would not contain multiple strains. :shrug:

Monoculture in a broader biological context means a single species in a given area (most farmers use monoculture of a single plant species) but as this is a mycology site I would personally avoid mixing up people with this terminology caps.


--------------------





Trade List




Extras: Filter Print Post Top
OfflineFishLevelMidnight
Aquaman
Male User Gallery


Registered: 09/01/17
Posts: 2,328
Last seen: 19 days, 12 hours
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47] * 1
    #25888118 - 03/21/19 01:11 PM (5 years, 1 month ago)

Quote:

Boogieman47 said:
Find peoples pictures of their isolates and look at them then tell me if you dont see different traits in the mushrooms... if it was a single strain they would all be the same height weight everything ..  I have never seen that




This is absolutely incorrect.

Fungi have advanced enough genetics to have growth variability based off environmental influences.

Even a genetic clone can have differences in those phenotypes that are affected by the environment. Environment can be microeenvironment also (so even though a "tub" is one environment a particular cell can have a different microenvironment based off distribution of spawn vs bulk, presence of bacteria beneficial or not, etc)


--------------------





Trade List




Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47] * 1
    #25888125 - 03/21/19 01:12 PM (5 years, 1 month ago)

Idk man... but not worth going on about... from my understanding, an MS culture contains hudreds(even thousands)of strains and is technically a monoculture if it's clean, a monoculture (as we use the term) is still multiple strains, but  performing together as one  (typically a clone culture),  and an isolate is a single set of genetics derived from isolation of a given monoculture... seems like it'd be nearly impossible to achieve without some serious equipment though... I like clone monocultures personally, and of the limited testing I've run, they typically outperform cultures with further isolation efforts (safely worded I suppose)

Extras: Filter Print Post Top
Invisiblefilthyknees
no coincidence
 User Gallery

Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Post Your Cultivation Picture of The Day [Re: FishLevelMidnight] * 1
    #25888126 - 03/21/19 01:12 PM (5 years, 1 month ago)

Liloldme has been pumping out isolate/monoculture pics consistently for awhile, I think he got bored posting because they all look identical.


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

Extras: Filter Print Post Top
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,712
Loc: Under your bed Flag
Re: Post Your Cultivation Picture of The Day [Re: FishLevelMidnight]
    #25888140 - 03/21/19 01:17 PM (5 years, 1 month ago)

Ok then I take that back... in my mind I do understand about the phenotypes and characteristics of genetics but in the same sense if it was a single strain wouldnt it act more consistent then not? I mean I feel you on environmental and all that but still a single strain is a single strain but in that I may be confused ...


Yeah lil has some bad ass genetics but I'm just saying the argument is over the fact that we can still have multiple strains in a isolate...


Fk what up man hope you're all good I still feel bad about not being able to come see ya


--------------------

Extras: Filter Print Post Top
Invisiblefilthyknees
no coincidence
 User Gallery

Registered: 03/08/13
Posts: 6,283
Trusted Cultivator
Re: Post Your Cultivation Picture of The Day [Re: Boogieman47]
    #25888168 - 03/21/19 01:30 PM (5 years, 1 month ago)

Doing well, just talking about some bs with strangers who have too much free time like me.

I think the concensus is monoculture == isolate. If you want to consider an isolate any ms that has had an isolation done to be an isolate sure isolates can have multiple strains. I just don't think that's what anyone thinks of when talking about isolate.

I think maybe that's what's confusing people about what you're saying.

Sad not to post any pics right now but I feel something coming in the future...

:peace:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

Extras: Filter Print Post Top
OfflineMr. Funguy
Quasi Frodo
Male User Gallery

Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 4 years, 6 months
Re: Post Your Cultivation Picture of The Day [Re: filthyknees]
    #25888227 - 03/21/19 01:58 PM (5 years, 1 month ago)

:jah:


--------------------

Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 7 days, 8 hours
Trusted Cultivator
Re: Post Your Cultivation Picture of The Day [Re: filthyknees]
    #25888233 - 03/21/19 02:01 PM (5 years, 1 month ago)

a mono culture is a single homogeneous culture without diversity.
isolates are hunted traits you have bred together in hope of them appearing of fruits. getting these traits to consistently appear is what is referred to as "stabilizing" I use mono cultures to make iso's (however until I got my microscope I more than likely was just breeding iso's)
i am however self taught so i cant say with 100% confidence that this is the accepted vernaculars but in my mind it stands to reason, and is supported by defintion


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

Edited by Jakeoncid419 (03/21/19 02:33 PM)

Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Post Your Cultivation Picture of The Day [Re: filthyknees] * 1
    #25888245 - 03/21/19 02:10 PM (5 years, 1 month ago)

Lol... monoculture-one culture (literal translation)... isolate- isolated (1 set)genetics (logical deduction)... seems straight forward to me :shrug: I've put it 3 different ways and you still twisted it up lmao

Try again here... what I'm saying is:

A clean MS culture IS a monoculture, NOT an isolate: as it contains only one culture... this is technically speaking, and not generally the terminology used in mycology(as we call it an MS culture to distinguish the low-level of isolation applied)

A monoculture  (generally speaking-in mycology) is a term applied to a culture containing multiple strains performing together as one; like in a clone culture

An isolate- a single set of genetics... one (isolated
(As the term implies)) strain

How is this NOT the case? Lmao, is what it is,  but damn

Well put jake

Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 7 days, 8 hours
Trusted Cultivator
Re: Post Your Cultivation Picture of The Day [Re: Caps McGee] * 1
    #25888253 - 03/21/19 02:16 PM (5 years, 1 month ago)

Quote:

Caps McGee said:
Lol... monoculture-one culture (literal translation)... isolate- isolated (1 set)genetics (logical deduction)... seems straight forward to me :shrug: I've put it 3 different ways and you still twisted it up lmao

Try again here... what I'm saying is:

A clean MS culture IS a monoculture, NOT an isolate: as it contains only one culture... this is technically speaking, and not generally the terminology used in mycology(as we call it an MS culture to distinguish the low-level of isolation applied)

A monoculture  (generally speaking-in mycology) is a term applied to a culture containing multiple strains performing together as one; like in a clone culture

An isolate- a single set of genetics... one (isolated
(As the term implies)) strain

How is this NOT the case? Lmao, is what it is,  but damn

Well put jake




I would not refer to ms as mono culture tho not in anysense. its the exact opposite. I belive those mycologists that you heard used it for a multispore were mistaken. a multispore has only one stain of mushroom but many random substrains (some not even fruiters) . a mono culture has one substrain, a iso has an array of selected ideal substrains

I supposed you MS is comprised of lots of mono cultures. if you mean mono culture as in ther is only one stain/species on the plate then yes I suppose you could say you isolated the strain but it still would not be an isolate, it would be a monostrain


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

Edited by Jakeoncid419 (03/21/19 02:30 PM)

Extras: Filter Print Post Top
Offlinehamonz
Junglist
 User Gallery


Registered: 09/07/18
Posts: 178
Last seen: 10 months, 13 days
Re: Post Your Cultivation Picture of The Day [Re: Caps McGee] * 3
    #25888259 - 03/21/19 02:19 PM (5 years, 1 month ago)

Its been a while since I've posted, but I have not been lazy :smile:




:bunnypeace:

Extras: Filter Print Post Top
OfflineJakeoncid419
Follow me.....
Male User Gallery


Registered: 06/26/18
Posts: 4,390
Loc: Basel Switzerland
Last seen: 7 days, 8 hours
Trusted Cultivator
Re: Post Your Cultivation Picture of The Day [Re: hamonz] * 1
    #25888294 - 03/21/19 02:39 PM (5 years, 1 month ago)

speaking of ms here are some pan MS tays I running in my pursuit of good traits for iso's thes are pan aus and my mystery pan just starting to come up.


--------------------
Natural omt/detox
online  pant cult classes available last Saturday of every month go to buymeacoffee.com/jakeoncid to sign up (1 on 1 consultations also available
JOC PAN TEK
CORDYCEPS MILITARIS

EXOTICS
[

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 4056 | 4057 | 4058 | 4059 | 4060 | 4061 | 4062 | 4063 | 4064 | 4065 | 4066 | 4067 | 4068 | 4069 | 4070 | 4071 | 4072 | 4073 | 4074 | 4075 | 4076 | Next > | Last >

Shop: OlympusMyco.com Olympus Myco Bulk Substrate   Mushroom-Hut Mono Tub Substrate   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   Myyco.com APE Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Post deleted by Administrator
( 1 2 3 4 all )
Alien 25,249 72 08/26/02 07:55 AM
by kingkc
* Post Your New Tools Of The Trade.
( 1 2 3 4 ... 627 628 )
hamloaf 398,123 12,559 05/17/24 10:41 PM
by ReverendMyc
* Official Ps. Subtropicalis/Semperviva Thread
( 1 2 3 4 ... 268 269 )
nosf3r4tu 160,965 5,361 05/16/24 12:15 PM
by YoshiTrainer
* Pf tek into shoebox first time(pictures included) wizardontheshit 134 4 05/15/24 05:03 PM
by PBJ710
* would this mulcher be good for straw?? DeadPhan 438 3 05/30/13 09:05 AM
by Satori23
* Re: simple question about laminar flow hoods Anonymous 735 2 04/15/00 11:07 AM
by Anonymous
* Pictures of my latest attempt to produce clean spawn. I welcome any experienced cultivator input. GrowimanS 79 5 05/14/24 10:29 AM
by tripdawg420
* Printing Semperviva Javadog 2,768 17 01/23/11 01:48 PM
by Javadog

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,390,094 topic views. 13 members, 68 guests and 37 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.079 seconds spending 0.062 seconds on 14 queries.