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Invisiblejuniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
Re: Cultivation General Discussion [Re: staytrippy420]
    #25886941 - 03/20/19 10:54 PM (5 years, 9 days ago)

First step is probably don't get pre-poured plates :uhoh: lol.

But seriously, search for some clone TEKs, there are some good ones on here. I think FrankHorrigan's is what I use, don't recall


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To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas

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Offlinegizmodo
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Re: Cultivation General Discussion [Re: staytrippy420] * 1
    #25886943 - 03/20/19 10:56 PM (5 years, 9 days ago)

Review your understanding of sterile procedure, look at information on contamination on plates, hidden contamination too.
Look at peoples pictures of contaminated plates and read their questions and look at the answers for common issues regarding agar where you can find it.
Read, learn things for yourself and do everything yourself or you'll likely end up disappointed and spending more money than you should.
Look into everything enough to know that you're ready for the entire process without much question.

Contamination Forum

Also.... :whathesaid:


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits

Edited by gizmodo (03/20/19 10:57 PM)

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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: gizmodo]
    #25886968 - 03/20/19 11:19 PM (5 years, 9 days ago)

Is the contamination forum less dead nowadays? I keep seeing people mention it, but I've had more luck understanding what's going on here in mush cult.

You can cook agar without a pc no problem. Just sterilize some tools and dont let anything not sterile over any media. It just sterile procedure


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Invisiblestaytrippy420
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25886996 - 03/20/19 11:49 PM (5 years, 9 days ago)

lol I know, know. it was only 4 bucks a plate tho... figured it was worth a shot.
I've been lurking the contam forum here and there but I'll get my study on. Thanks fellas


--------------------
Tek's I use

LAGM2020

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Invisiblesh4d0ws
LSx
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Registered: 02/26/08
Posts: 12,086
Re: Cultivation General Discussion [Re: staytrippy420]
    #25887111 - 03/21/19 01:48 AM (5 years, 9 days ago)

$4 a plate can add up fast if you're growing in bulk...but you should learn how to mix agar yourself. You should definitely get a PC...then Start with some pasty plates or use 125mL glass jars with a filter for gas exchange. Then you can make no pour ones yourself. It'll be way cheaper, cheap enough that it will pay off the PC you buy, because then you won't be paying $4 a plate :tongue2:


Hopefully they are a proper nutrient ratio and actually sterile. Are they just in petri dishes? Or some kind of sealed container?


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Offlinemuhfreedumb
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Re: Cultivation General Discussion [Re: vikingsc]
    #25887194 - 03/21/19 03:50 AM (5 years, 9 days ago)

At 4$ a plate my PC would have already paid for itself within 1 round of pasty plates. At least the high cost will motivate you to not get lazy with your sterile procedure :laugh:

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OfflineLyh
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Registered: 05/16/18
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Re: Cultivation General Discussion [Re: muhfreedumb]
    #25887213 - 03/21/19 04:31 AM (5 years, 9 days ago)

:whatshesaid: what they said. I only started working with agar this year, but it's been great. Using pastyplates like sh4d0ws explained. The glasses came 25 for 14€. Agar powder is cheap af and the nutrients are free if you keep the water from boiling grains. The best part is that you can use them over and over with just paying for making the agar.


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´

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InvisibleCaps McGee
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Registered: 10/28/17
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Re: Cultivation General Discussion [Re: Lyh]
    #25887427 - 03/21/19 08:09 AM (5 years, 9 days ago)

Anyone know where to source just one large HEPA?

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Offlinedmppb
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25887560 - 03/21/19 09:26 AM (5 years, 9 days ago)

Quote:

tryptkaloids said:
Is the contamination forum less dead nowadays? I keep seeing people mention it, but I've had more luck understanding what's going on here in mush cult.






equally dead i think ? and me too ; although i will post this there for sure to ask for id of the contam

i'm having a hard time telling apart my myc from contam , this outter edges maaaan

is it really mold or bacteria growing below my myc ? or is it the first stages of the advance of my myc ? i'm talking about the fine tiny threads leading the mycelium

i just did transfers yesterday and used PDA with karo, i'm hoping to see clearer growth maybe ?



overrun by the tiny threads  :



i've let it go before and maybe the mycelium wins the battle but still carries the contam to grain  , maybe this had it and i didn't noticed ?



--------------------
...and when you loose control ; you'll reap the harvest you have sown.

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Offlinegizmodo
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Re: Cultivation General Discussion [Re: dmppb]
    #25887565 - 03/21/19 09:28 AM (5 years, 9 days ago)

ONLY 4 bucks a plate? :uhoh:


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits

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Offlinedmppb
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Re: Cultivation General Discussion [Re: gizmodo]
    #25887663 - 03/21/19 10:08 AM (5 years, 9 days ago)

Quote:

gizmodo said:
ONLY 4 bucks a plate? :uhoh:




:lol:

as posted in the contamination forum , any takers on the id of this contam ?

it just occurred to me that i should isolate it and see it better duh...

but anyway , pretty sure someone is already an expert ; :pipesmoke::cheers:

Quote:

dmppb said:
hello !

so my tubs were colonizing and pinning but then stopping and aborting , so after checking and testing my plates and jars realized everything was dirty , now i'm at the cleanup process but i'm having a hard time getting rid of what  i think is a contamination ;

bacterial pin


spawn that looked "ok"



tubs were quick to colonize pins at day 14



bad flushes :



yellow drop and uncolonized kernel :




so , culprits  and things i know i did wrong

-did not vent my PC while pc'ing grain jars ( how much did this affected? )
-did not check my jars properly and that yellow drop was most likely present along with that single uncolonized kernel
-my agar plates had hidden bacteria or mold but were not visible because of the persistent use of peroxide , then passed to grain and so on.
- these outter edge tiny threads were present but were mistaken for myc.



so question ; what are these tiny threads and how do i get rid of them ? i can understand the rest is bacteria caused by poor sterile tek.


gracias !!!




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...and when you loose control ; you'll reap the harvest you have sown.

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InvisibleYesum
Furry as Fuc
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Registered: 11/05/12
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Re: Cultivation General Discussion [Re: dmppb] * 1
    #25887783 - 03/21/19 11:00 AM (5 years, 9 days ago)

Those plates look clean dude. Good thing too for 4$ a plate.


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Offlinetryptkaloids
Learner
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Registered: 02/08/15
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Re: Cultivation General Discussion [Re: dmppb]
    #25887840 - 03/21/19 11:22 AM (5 years, 9 days ago)

I'm not going to touch the grain jars.. but the plates I think have something hidden. the leading edge likes to be thin. but it should be uniform and the same width from the main culture


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleBoogieman47
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Registered: 03/05/16
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25887862 - 03/21/19 11:30 AM (5 years, 9 days ago)

The leading edge when its transparent is just the myc eating through the agar I use transfer that alot ...


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Boogieman47]
    #25887865 - 03/21/19 11:31 AM (5 years, 9 days ago)

yea, but there's an offshoot that sketches me out


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25887880 - 03/21/19 11:37 AM (5 years, 9 days ago)

Oh yeah on that last plate?? That one shows a crazy growth I thought you meant the small ring that comes off the colonized myc


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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: Boogieman47]
    #25887896 - 03/21/19 11:42 AM (5 years, 9 days ago)

they all have it, its just hidden by the glare


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Offlinedmppb
On course


Registered: 11/11/11
Posts: 1,043
Loc: universe
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25887916 - 03/21/19 11:54 AM (5 years, 9 days ago)

Quote:

tryptkaloids said:
yea, but there's an offshoot that sketches me out





exactly !
some may have it a little in front of myc and it's almost not visible , and growing almost just as fast , and then this offshoot looks all wrong and very similar to this shit  :



i did transfers yesterday and will keep looking out  ?      :shrug:


--------------------
...and when you loose control ; you'll reap the harvest you have sown.

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Offlinetryptkaloids
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Re: Cultivation General Discussion [Re: dmppb]
    #25887972 - 03/21/19 12:18 PM (5 years, 9 days ago)

that's kindof all you can do. let them grow out to dime size and hot pour


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflineShaperDreaming
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25888242 - 03/21/19 02:08 PM (5 years, 9 days ago)

Quote:

Tweeq said:
Hey @sd so when can you speak of a new variety? I am confused about that. I read something about after f6?

There is this Dutch vendor that comes up with all kinds of 'strains' that are hybrids. But on fsre I read that this was a f1 hybrid. So are those stable non degenerative varieties you think? Got the links for you if you like but I don't think I am allowed to share those here



Honestly, I'm not sure the technicality here. I believe that Rusty Whyte was on F9 F7 (thanks Tryp) when it was released. I imagine it will take a while to get to that point where it's putting out consistent phenotypes from what I'm looking for, and will be slow AF.

Not sure what's going on with that vendor. A hybrid of what exactly? A cube is a cube, so I'm just aiming for phenotypes like color, size, potency, etc.

Quote:

Lyh said:
Quote:

ShaperDreaming said:
Quote:

sh4d0ws said:
Who here has tried to grow APE from swab? Is everyones experience that they get normal looking cubes from APE spores?



Here were my results:
https://www.shroomery.org/forums/showflat.php/Number/25871458#25871458

Took a long ass time of cleaning them up to get there. Flush 2 just finished and had the same blue caps. I'm hoping to make a variety out of these weirdos!




They look really cool! How is the potency? Is it the same as normal APE?



Haven't had a chance yet. Test coming this weekend. Will likely report on Monday. If it's APE/PE level, I'm going to be super excited. They keep coming up with blue caps, and they flush almost instantly/constantly. First flush only had 5 days of down time between flushes. Even if the weight is low, if they're pumping out flushes at this rate they're still a winner, just with more work.

Edited by ShaperDreaming (03/21/19 02:59 PM)

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