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Offlinejames.lucas.78
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Registered: 03/14/19
Posts: 5
Last seen: 17 hours, 43 minutes
My Tek * 1
    #25874636 - 03/14/19 10:02 PM (6 days, 7 hours ago)

I started this hobby only recently, here's my Cubensis tek on a shoestring, in case anybody is interested.

Equipment:
- Still air box, cheap big plastic tote, cut out two arm holes
- Pressure cooker, borrowed from a friend
- Diy inoculation loop, as in, piece of ss wire stuck in a pen with a big nail
- Mason jars, .25 qt, .5 qt, 1 qt
- Mason jar lids, punched a hole in each with a nail, filled with piece of cotton
- Plastic tubs 20 qt
- Microporous tape

Consumables
- Aluminum foil
- Iso propyl ethanol
- Disinfectant wipes and spray
- Agar
- Malt extract or unfermented beer (wort)
- Bread Yeast
- Grain
- Chalk powder (Calcium Carbonate)
- Coco coir and/or staw

Agar Procedure
- Make a broth of 20g/20g agar/malt extract per litre, I only made 200ml for my batch of 10 jars. Add pinch of yeast, simmer a few minutes
- Pour broth in .25 qt mason jars, just enough to cover the bottom surface, close with punched cotton stuffed lids
- Cover each jar with alu foil
- Pressure cook jars at 15 psi for an hour or so, let it cool
- Boil inoculation loop in a pot of water, take it out of boiling water, cover with disinfectant wipe
- In still air box, put jars, inoculation loop, lighter, spore print. Wipe everything including the box with disinfectant wipes beforehand.
- Disinfectant spray in the box, let it sit for a few minutes
- wash hands, disinfect hands
- Heat inoculation loop tip with lighter, place in agar to cool, pick up some spores from print, place in jar. Repeat for all jars.
- Put jars away upside down in a warm closet.
- Puffy white mycelium grows on top of the agar within 3 to 6 days.

Liquid Culture, optional step
- Make a broth of 40g malt extract per litre with a pinch of yeast, simmer a few minutes, fill a .5 qt mason jar, add punched cotton lid, cover with foil
- Pressure cook for an hour or so, let it cool
- Take a colonized agar jar, put in still air box together with LC jar and a knife, disinfect everything (box, jars, knife, hands)
- Disinfectant spray in the box, let it sit for a few minutes
- Open the agar jar, cut the agar in little pieces, dump in LC jar, close it.
- Put LC in warm closet for a week, swirl daily
- Put the LC back in the still air box after a week. Fill up sterile syringes with the liquid from the jar. Seal and store in fridge.

Grain
- Bring water to boil, pour water on whole grain (rye, wheat, oats, etc. all of them work), let it soak for a while, I do this over night
- Dump in strainer, let it drip out for a few hours
- Add pinch of chalk, stir
- Fill .5 qt jars up to 50% to 75% full
- Add punched cotton lids, cover with foil
- Pressure cook for 90 minutes, cool off
- In still air box, after disinfecting everything, transfer either the contents of a colonized agar jar, or 1ml of LC to each grain jar, shake
- Put away in warm closet. Shake at 50% colonization.
- Wait til all grain kernels have a white hue. Don't let it fully colonize. This takes about a week.
- Repeat from the beginning, now with 1 qt jars. Transfer grain to grain.
- You should end up with 1 qt jars of fully colonized grain

Tubs
- Drill holes at 4.5" from the bottom up. Also drill holes just under the rim. Cover holes with microporous tape
- Soak coco and/or staw in plenty of hot water
- Either pasteurize, treat with CaOH, or pressure cook. Bring back to field capacity by squeezing water out with hands.
- Clean and disinfect tubs and hands
- Mix 1 qt jar with substrate in the tub with your hands, cover with a layer or just substrate, get it up to 4"
- Put away in a warm closet for 10 days or so, the substrate should then be fully colonized
- Bring into a room with indirect sunlight, around 24C, wait another week or so, pins should appear.

That's where I am. It works for me.
I think, compared to other teks I read, this is plain and simple. My most recent tub grows on CaOH treated straw and works just fine. Please comment.
I don't line my tubs with plastic bags, I don't see the benefit.
Here's a pic from today



Edited by james.lucas.78 (03/14/19 10:20 PM)


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OfflineTormatoS
It’s OK!! I’m a Limo Driver!! 😁
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Re: My Tek [Re: james.lucas.78] * 2
    #25874695 - 03/14/19 10:30 PM (6 days, 7 hours ago)

Kinda looks like you just took some other TEKs and made a write up....I mean if it works for you great, but I wouldn't call this a TEK. :shrug:


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflinePsilosopherr
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Re: My Tek [Re: Tormato]
    #25874703 - 03/14/19 10:34 PM (6 days, 7 hours ago)

You've been growing cubes on lime pasteurized straw? Awesome. I was wondering if people did that. :thumbup:

Any other unique methods I missed in that block?


Edited by Psilosopherr (03/14/19 10:34 PM)


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Invisiblestaytrippy420
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Registered: 03/23/13
Posts: 330
Loc: BC Flag
Re: My Tek [Re: Psilosopherr]
    #25874717 - 03/14/19 10:38 PM (6 days, 7 hours ago)

Not really a tek but still a dope post. I'm going to start fucking around with agar soon and this helped break down what I will need to get started.


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Offlinejames.lucas.78
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Registered: 03/14/19
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Re: My Tek [Re: staytrippy420] * 1
    #25874724 - 03/14/19 10:45 PM (6 days, 7 hours ago)

Tek, method, procedure, semantics. This is how I do it.


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OfflinePsilosopherr
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Re: My Tek [Re: james.lucas.78] * 1
    #25874728 - 03/14/19 10:49 PM (6 days, 6 hours ago)

Absolutely. We can be a little picky about vernacular on here. Usually re-hashings of long standing teks aren't called teks. But I say this purely as an FYI and see what you're saying.

Welcome to the shroomery! Nice first post. I don't think I'd ever heard of somebody using lime pasteurization for cubes before. Maybe you could do a proper tek on that because I don't think we even have a  normal lime pasteurization tek for gourmet stuff


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OfflineTormatoS
It’s OK!! I’m a Limo Driver!! 😁
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Folding@home Statistics
Registered: 07/01/17
Posts: 4,003
Loc: Montagar
Last seen: 20 hours, 16 minutes
Re: My Tek [Re: james.lucas.78]
    #25874751 - 03/14/19 11:08 PM (6 days, 6 hours ago)

Quote:

james.lucas.78 said:
Tek, method, procedure, semantics. This is how I do it.



I hear ya brotha...not knocking ya. Glad you were  able to research and found a combo that works for you! I'm with psilosopherr nice lime pasteurization for cubes....you don't see that a lot! :thumbup:


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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Offlinejames.lucas.78
Stranger

Registered: 03/14/19
Posts: 5
Last seen: 17 hours, 43 minutes
Re: My Tek [Re: Tormato]
    #25874764 - 03/14/19 11:17 PM (6 days, 6 hours ago)

Here's my lime treated straw with myc coming through. Also taken today.
For the price of a coco brick, I can buy 100x the amount of straw. I'm surprised this is so rare.



Edited by james.lucas.78 (03/14/19 11:20 PM)


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OfflinePsilosopherr
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Re: My Tek [Re: james.lucas.78]
    #25874776 - 03/14/19 11:24 PM (6 days, 6 hours ago)

Shweet. When you're doing the lime bath do you use hot water or cold/tepid? How long do you soak it in the solution?


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Offlinejames.lucas.78
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Registered: 03/14/19
Posts: 5
Last seen: 17 hours, 43 minutes
Re: My Tek [Re: Psilosopherr]
    #25874793 - 03/14/19 11:43 PM (6 days, 6 hours ago)

I tried to follow Paul's method, page 175. I used hot tap water to better dissolve the hydrated lime.


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InvisibleHobbyist
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Registered: 08/15/10
Posts: 486
Re: My Tek [Re: james.lucas.78]
    #25874911 - 03/15/19 12:58 AM (6 days, 4 hours ago)

Lookin good.  Welcome!


--------------------
Everything i say is completely hypothetical...


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OfflineC2018R
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I'm a teapot

Registered: 12/22/18
Posts: 4
Last seen: 17 hours, 31 minutes
Re: My Tek [Re: james.lucas.78]
    #25885434 - 03/20/19 11:55 AM (17 hours, 50 minutes ago)

Thanks for the share!


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