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SupaThaRipper
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Re: Official Misc EXOTIC Thread [Re: kirkeng]
#28587394 - 12/17/23 08:48 PM (1 month, 10 days ago) |
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I hope so lol. I just did an LC test plate of it and it was looking a bit bacterial but not like that. Did some transfers today and going to see how it turns out
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wavyedge

Registered: 09/24/11
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Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 2
#28587397 - 12/17/23 08:51 PM (1 month, 10 days ago) |
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What kind of agar?

Asking because this was weak PDA. Don't mind the mold in the top, came after several transfers and being sloppy.
The transfer plate is grain soak agar:

And the grain water ones show discoloration like that sometimes.
Edited by wavyedge (12/17/23 09:07 PM)
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kirkeng
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Re: Official Misc EXOTIC Thread [Re: wavyedge]
#28587405 - 12/17/23 08:57 PM (1 month, 10 days ago) |
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Hmm ok this is mea I want to say like 1.5% but canโt remember how strong I made it this time. Seems the discoloration hasnโt followed with the transfer so far. But if typical the shits beautiful.
Edit: looking back a few pages I knew I remembered seeing this coloration on them before, nichrome showed it nicely.
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alaskappalachian
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 3
#28587425 - 12/17/23 09:11 PM (1 month, 10 days ago) |
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Quote:
kirkeng said: Man this neoxalapensis germ plate looks gnarly as hell. Making some wild colors. Transferring brown spots is lending some nice white mycelium though.

If I recall this browning is typical though?
Thanks for posting this. Like 4-5 days after incoulating my first two test plates with neox lc the only signs of potential growth I can perceive are three little raised dots that looked white at first then started to turn brownish. Like pencil dot tiny so will see but makes sense. Did several subsequent dishes with different agar recipes so it'll be fun to see what comes of it.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Speaking of neoxalapensis,
Blob transfers (taken from the blob) are growing decently.


And I'm not doing the big post yet because I want to see more growth, but:
Starter mix:




Which is on-par for the pan cyan (wild coast) growth on manure:

Baeos are still lagging.
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wavyedge

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Re: Official Misc EXOTIC Thread [Re: wavyedge] 3
#28587446 - 12/17/23 09:30 PM (1 month, 10 days ago) |
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And following that I am seeing decent growth jumping off the agar into this millet+perlite+verm abomination I cooked up.

Better growth speed overall, still shakeable. We'll see I have other T1s that are fast so it might just be down to finding the best isolate.
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kirkeng
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28587450 - 12/17/23 09:33 PM (1 month, 10 days ago) |
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Those look promising! Would you say those have been the best mix so far?
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 1
#28587460 - 12/17/23 09:38 PM (1 month, 10 days ago) |
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So far, other than this, I've only tried oats. And they were likely on the dry side, but that was a different isolate.
Am I'm using the term "isolate" loosely here because I just mean I cut a segment from an MS plate, transferred, grew it out a bit and put that to grain. I numbered the isolates that I took and right now 1, 2 and 5 seem to grow at a decent rate. And that seems to include the blob clones from 2.
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scapo
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 6
#28587482 - 12/17/23 10:06 PM (1 month, 10 days ago) |
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This is normal! I get browning on most of my Cordisporae species, and they'll produce clean LC's from tinted plates. My fagicola that is heavy brown transferred white as well.




Fagicola transfer (bottom of plate and two plates stacked so a little funky but it's clean)
Edited by scapo (12/17/23 10:07 PM)
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kirkeng
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Re: Official Misc EXOTIC Thread [Re: scapo] 1
#28587485 - 12/17/23 10:08 PM (1 month, 10 days ago) |
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Excellent thank you for confirmation! I have to say the color is amazing!
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wavyedge

Registered: 09/24/11
Posts: 362
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Re: Official Misc EXOTIC Thread [Re: scapo]
#28587487 - 12/17/23 10:10 PM (1 month, 10 days ago) |
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So maybe white strains outrunning the dark? Almost chocolate colors there.
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scapo
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 4
#28587491 - 12/17/23 10:13 PM (1 month, 10 days ago) |
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Ime, the white will turn dark over time, and eventually you can isolate myc that will do it slower or not at all. I make 2-4 plates from each transfer and maybe one of them will be white most of the time. I have observed this with neoxalapensis and fagicola so far, heimii and yungensis tint dark as well but I have yet to get white from them. Banderillensis was the quickest to transfer white. I have had neoxalapensis tint dark like this in LC as well. Hoogshagenii also does this which is fun!
Interestingly I have an azurescens that turns darker than anything I have seen so far (only in LC). It's as dark as iced tea!
Edited by scapo (12/17/23 10:26 PM)
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wavyedge

Registered: 09/24/11
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Re: Official Misc EXOTIC Thread [Re: scapo] 1
#28587495 - 12/17/23 10:17 PM (1 month, 10 days ago) |
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Cool. I am pleased so far with how this work has gone, feels satisfying to see some things in common.
But I want to test my assumptions. I hope I'm not growing mold. The blob/maybe pins I cloned stoke my hopes lol. Scapo, what do you know about moving to grains or bulk substrates, insofar as that has been explored at this point?
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scapo
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 3
#28587499 - 12/17/23 10:20 PM (1 month, 10 days ago) |
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They're all a fan of brf cakes, I do not use grains anymore. I had several exotics not take off on it so I searched for alternatives and this was it! Here's a banderillensis cake going to manure/mulch/fuel pellets/hemp fibers/verm tonight!

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scapo
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Re: Official Misc EXOTIC Thread [Re: inski] 2
#28587506 - 12/17/23 10:24 PM (1 month, 10 days ago) |
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Quote:
inski said: Psilocybe aucklandiae on MEA, two different isolates from multispore germinations.

P. angulospora, this is a clone from a wild collected specimen in 2019, the dish was stored at room temperature and has revived nicely, both of these were transferred from the same culture, the first is on half strength MEA, the second on full strength MEA .
 I'm currently testing this one on a few different agar recipes as it is a challenging species to work with, very slow irregular growth, more images to come on the different recipes.
P. weraroa on standard MEA, the first one here is a stipe tissue clone from a wild collected specimen and the second is an isolate from a multispore germination from the same collection.

This comment is excellent. I would love to see a thread dedicated to representative agar photos of exotics.
I should take more time to photograph my plates when they're earlier in their growth like this. I have only one shot as I usually do not have my phone handy with transfers, Ps. subaeruginascens on R2A agar
 Subaeruginascens
Edited by scapo (12/17/23 10:27 PM)
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SupaThaRipper
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Re: Official Misc EXOTIC Thread [Re: scapo]
#28587509 - 12/17/23 10:26 PM (1 month, 10 days ago) |
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Why the micropore tape 90% around the lid?
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wavyedge

Registered: 09/24/11
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Re: Official Misc EXOTIC Thread [Re: scapo]
#28587510 - 12/17/23 10:27 PM (1 month, 10 days ago) |
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Very nice. I'm trying something similar to brf cake with my millet + perlite + verm thing. Was going for a lower nutrient density, and it's going decent. How long did it take to colonize that brf cake?
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scapo
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Quote:
SupaThaRipper said: Why the micropore tape 90% around the lid?
I kept having issues with them drying out on me! They take 1-2 months to colonize unfortunately.
I now prefer bags - you get more spawn faster because you can break and shake, and you can inject a little more LC without it being too much
Edited by scapo (12/17/23 10:30 PM)
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Quote:
SupaThaRipper said: Dude how do you take these pictures? It literally looks like you have a wedge just growing out on a nice table ๐คฃ
These are beautiful man!
Thanks SupaThaRipper, so basically I sat the dish on my table and used four Godox MF12 macro flash units with the modeling function which is just continuous LED light, that's why you can see the light coming from each corner.
Next time I'll use a sheet of black felt for the background and try to minimise the intensity of light coming from the corners, I just used my phone but usually use the dslr, these are through the ziplock and dish so not so sharp but you can get an idea of the type of growth from each species ๐
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inski
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Re: Official Misc EXOTIC Thread [Re: scapo] 1
#28587541 - 12/17/23 11:22 PM (1 month, 9 days ago) |
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Quote:
scapo said:
Quote:
inski said: Psilocybe aucklandiae on MEA, two different isolates from multispore germinations.

P. angulospora, this is a clone from a wild collected specimen in 2019, the dish was stored at room temperature and has revived nicely, both of these were transferred from the same culture, the first is on half strength MEA, the second on full strength MEA .
 I'm currently testing this one on a few different agar recipes as it is a challenging species to work with, very slow irregular growth, more images to come on the different recipes.
P. weraroa on standard MEA, the first one here is a stipe tissue clone from a wild collected specimen and the second is an isolate from a multispore germination from the same collection.

This comment is excellent. I would love to see a thread dedicated to representative agar photos of exotics.
I should take more time to photograph my plates when they're earlier in their growth like this. I have only one shot as I usually do not have my phone handy with transfers, Ps. subaeruginascens on R2A agar
 Subaeruginascens
Thanks scape, that's a great idea for a thread ๐
That looks like a very nice P. subaeruginascens culture, well done ๐
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