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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Trying out sterilized subs in these takeout dishes:

- neoxalapensis - baeocystis - pan bisporus - pan cyan - semps
Across these substrates:
- Hardwood sawdust - composted manure - straw pellets + coir (2:1) - worm castings - starter mix (potting soil) - mulch - rye grass seed - Apple wood chips
All brought to field capacity (wood was soaked for a few days, rye grass seed was cooked as grain) as best I could.
I sterilized the substrates in jars as I would do grain, and from there on treated them as sterile substrates, (sterilized tools, work in front of hood).
maybe we find something neoxalapensis or baeocystis eats more readily. The semps and pans are for comparison. I also saved controls in case the 2hr PC wasn't enough.
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EniQma
Registered: 11/28/23
Posts: 470
Last seen: 5 hours, 56 minutes
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Re: Official Misc EXOTIC Thread [Re: wavyedge]
#28580912 - 12/13/23 07:32 AM (1 month, 14 days ago) |
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What have I stumbled upon here! A gold mine?
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 1 hour, 41 minutes
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28580936 - 12/13/23 07:48 AM (1 month, 14 days ago) |
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scapo
Scientist



Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 48 minutes
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 7
#28581144 - 12/13/23 11:25 AM (1 month, 14 days ago) |
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From my recent experiments I have learned that neoxalapensis (like baeos) does not care much for fresh chips! They work great in manure / mulch mixtures
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Ok, I think I remember lol. Made 8 Pf jars total.
2 with millet/verm only. One got Neoxalapensis and the other Niveotropicalis. 2 with millet/wfp/verm. One got Neoxalapensis and the other Niveotropicalis. 2 with BRF/pearl barley/flax/verm. One Neoxalapensis and the other Aztecorum. 2 with BRF/pearl barley/flax/wfp/verm. One got Niveotropicalis and the other Aztecorum.
Pretty standard recipe other than the change in ingredients. Everything would be in table spoons of each, except the millet which would be two. These will mostly be top fruiting experiments which is part of the reason I added the flax. That and I had some to use up lol. Most of my experience with flax is the mycelium eats it very slowly. Hopefully it will add to the longevity. Not real sure if the Aztecorum will top fruit either. Didn't have much luck with that the last time I worked with them. We'll see how it goes.
-------------------- "Luck favors the observant." - Workman
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Land Trout
Stranger



Registered: 01/08/18
Posts: 3,076
Last seen: 1 hour, 12 minutes
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Chosen one, how was your subaruginascens grow? You got them to fruit? And journal of it?
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: Land Trout]
#28581271 - 12/13/23 12:59 PM (1 month, 14 days ago) |
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I was offline during that time so no journal. It did ok though. Nothing too spectacular but some lanky.. for that species, fruits. Would have done much better outdoors, but at least a cold basement qualifies as indoors.
The Stuntzii were a little better, but also tall for the species. I'm thinking a result of the tub on both. Overall it was fairly close to what I've seen others do with them.
I'm probably gong to grab that drive adapter once the credit card clears this billing cycle. I always pay in full and I don't want to carry a balance lol. The first drive is a 2TB of which probably a quarter of it is the music and pics I want out of it. The other isn't that big but it's full of stuff too. Years of my life in those damn little boxes.
-------------------- "Luck favors the observant." - Workman
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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 Psilocybe Zapotecorum Texolo day 19 Top of the pile for this weekend's bout. I think I'm going to try the cryo vials, put some to grain and LC. It's moving fast.
Well. Faster than some anyways.  Banderillensis making.. bands.
 Psilocybe Banderillensis day 19
Weraroa making..

 Psilocybe Weraroa day 19
Angulospora is simply graceful.
 Psilocybe Angulospora day 19
All the slow pokes are a little over an inch in diameter and going back in the box for a while. Got other shit goin on.
-------------------- "Luck favors the observant." - Workman
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Nice set of cultures you've got there chosen, the P. weraroa is looking a little unusual, is it from a swab?
The P. angulospora is looking really uniform, did you do many transfers to get to this stage or was it like that to begin with? It usually produces very irregular growth even after quite a few transfers and regardless of what starting material is used, swabs, fresh gill fragments or clones from stipe tissue. I should also have asked, as I just assumed previously but does your material originate from New Zealand or is it from Taiwan?
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: inski] 2
#28584602 - 12/15/23 10:26 PM (1 month, 12 days ago) |
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So it's not just me then lol. I thought the Weraroa looked a little odd too. I've had it before and it looked more like the perimeter, but it did jump off the swab in a similar fashion. This one came as wedges in a cryo vial so I wasn't expecting it. I don't recall my old culture doing that when transferring.
The Angulospora came the same way. I'm just taking my baby steps with this species, but I'm thinking this culture is fairly isolated. I had assumed it was from New Zealand but the trader has such an amazing collection that it wouldn't surprise me if it was from Taiwan.
I almost forgot this one. Acquired from the same trader who actually found this one while in Chile. I also have the Brazilian from another source but it's in clean up right now. Nothing major just cleaning up some bacterial spots before they become a problem.
 Psilocybe Caeruleoannulata Chile day 19
I can't get it to show up in the pics but it's sectoring in three different colors. I'm not familiar enough with this species to know if it's normal and Brazil hasn't progressed nearly as far to compare.
-------------------- "Luck favors the observant." - Workman
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kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
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Got some aztecorum, thaiaeru, and niveo-tropical is to chips today, hoping those start to take off on that. Also samuiensis is getting close to g2g.

Can’t wait to start attempting those. And after seeing all of chosens beautiful agar shots I had to take a stab at it myself with the macro lens. Happy with the outcome and going to start doing this more frequently because it was fun to do. Samuiensis t2:
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 1
#28585297 - 12/16/23 12:03 PM (1 month, 11 days ago) |
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It is fun to do.

I haven't bought a macro lens yet but it's one the list. I did figure out the right place and angle for this cheap ass motorola though. Does ok but I think it would be better with the lens.
Samuiensis is moving slow on grain for me. Feels that way anyways lol. I'm probably going to upload some of the LC and start testing in PF style jars soon. I need to get that one in vials too.
Shit.
-------------------- "Luck favors the observant." - Workman
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kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
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Yeah mine has been slow as well, I think it’s taken like 3ish weeks to get to here? Maybe longer, Can’t remember as I didn’t date anything. My first grain drop was even slower though and still isn’t close to what this one is at. First one was Milo, which I prep on the dry side, second was rye which has more moisture so maybe that’s what caused it for me, though second one was a different selection as well so maybe poor selection at first. Not gunna lie was hoping they’d be a faster one, oh we’ll patience is the name of the game I suppose…
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Pnin
Riz Gukgak


Registered: 07/18/23
Posts: 395
Last seen: 3 hours, 20 minutes
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 1
#28585358 - 12/16/23 01:04 PM (1 month, 11 days ago) |
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Alright ya'll. What should I do with this pint of Ps. samuiensis? I'm going out of town in like six days, but this jar has already been sitting too long imo.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿 >my end of 2023 grow journal<
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Official Misc EXOTIC Thread [Re: Pnin] 14
#28585728 - 12/16/23 05:48 PM (1 month, 11 days ago) |
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Psilocybe aucklandiae on MEA, two different isolates from multispore germinations.

P. angulospora, this is a clone from a wild collected specimen in 2019, the dish was stored at room temperature and has revived nicely, both of these were transferred from the same culture, the first is on half strength MEA, the second on full strength MEA .
 I'm currently testing this one on a few different agar recipes as it is a challenging species to work with, very slow irregular growth, more images to come on the different recipes.
P. weraroa on standard MEA, the first one here is a stipe tissue clone from a wild collected specimen and the second is an isolate from a multispore germination from the same collection.
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Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,476
Loc: Zone 5
Last seen: 40 seconds
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Re: Official Misc EXOTIC Thread [Re: inski]
#28585733 - 12/16/23 05:53 PM (1 month, 11 days ago) |
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Beautiful plates.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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inski
Cortinariologist



Registered: 02/28/06
Posts: 5,720
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Re: Official Misc EXOTIC Thread [Re: Nichrome]
#28585745 - 12/16/23 06:00 PM (1 month, 11 days ago) |
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Thanks Nichrome👍
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: Pnin]
#28586817 - 12/17/23 12:54 PM (1 month, 10 days ago) |
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Quote:
Pnin said: Alright ya'll. What should I do with this pint of Ps. samuiensis? I'm going out of town in like six days, but this jar has already been sitting too long imo.

How long are you going to be gone? Mine are major slow on millet. Might be spring before they are ready to spawn lol
Inski getting it done.
Edited by the_chosen_one (12/17/23 12:56 PM)
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
Last seen: 1 hour, 41 minutes
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Re: Official Misc EXOTIC Thread [Re: inski] 1
#28586874 - 12/17/23 01:39 PM (1 month, 10 days ago) |
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Dude how do you take these pictures? It literally looks like you have a wedge just growing out on a nice table 🤣
These are beautiful man!
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kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
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Man this neoxalapensis germ plate looks gnarly as hell. Making some wild colors. Transferring brown spots is lending some nice white mycelium though.

If I recall this browning is typical though?
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