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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Speaking of strange colors. 
 Psilocybe Banderillensis day 11. Hard to see in that one but change the light frequency and back light, different story.
 Psilocybe Banderillensis day 11 2400K backlit.
This is pretty normal with Fagicola complex. I actually worry if they take off too fast and too white. Typically mold.
-------------------- "Luck favors the observant." - Workman
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scapo
Scientist



Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 48 minutes
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scapo
Scientist



Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 48 minutes
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Quote:
the_chosen_one said: I haven't checked the size I've been receiving from the trades, but they are all the same size regardless of who sends. Look about the same as those. I was planning on sterilizing the same way. Got so dang many I might not have to buy any for a while. 
Started journal entries on several species with more to come. Figured I'd better get on it now lol.

You can actually get 1,2,4mL, and even more! I found that the 2mL suffices because it can fit 3-6 wedges with 1.5mL distilled water easy.
They're great because they can be sterilized prefilled with water and they are made for cryogenic temperatures, so there is no concern about their longevity at fridge temps
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scapo
Scientist



Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 48 minutes
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Quote:
SupaThaRipper said: 1.8 marked but they’re technically 2ml. And for distilled water storage cultures (or whatever they’re called..lol)
The method is referred to as "Castellani's method" which is used pretty widely in fungal research for the long term storage of mycelium!
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
Loc: USA
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Re: Official Misc EXOTIC Thread [Re: scapo]
#28573896 - 12/08/23 06:02 AM (1 month, 19 days ago) |
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I have the same boxes 😄 do you store yours in the fridge? I’ve just been leaving mine out. I’ve heard it doesn’t matter either way.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
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Re: Official Misc EXOTIC Thread [Re: scapo] 1
#28574080 - 12/08/23 08:48 AM (1 month, 19 days ago) |
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2ml, 100pcs w/holder
https://www.amazon.com/dp/B09DYWX8Q2/ref=sspa_mw_detail_0?ie=UTF8&psc=1&sp_csd=d2lkZ2V0TmFtZT1zcF9waG9uZV9kZXRhaWw
I'll fill them, screw on the caps then back them off a 1/4 turn. I'll stack them vertically in a 1/2 pint jar w/vented lid (spawn jar lid). PC them 30min w/my agar.
After cooling, in front of my HEPA, I tighten the lids all the way and leave them in the jar until needed.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: YoshiTrainer] 1
#28574085 - 12/08/23 08:52 AM (1 month, 19 days ago) |
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Nice, thx for the link. Been buying the bags of 2ml bottles but a box is nice to have for nearly the same price.
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28574617 - 12/08/23 04:05 PM (1 month, 19 days ago) |
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I bought one. Hey $17 with the coupon and a subscription. This is my first subscription mushroom item I have ever purchased. I'll see more in a few months. 
Thanks Yoshi!!
-------------------- "Luck favors the observant." - Workman
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Nichrome
I'm a torso!


Registered: 12/17/18
Posts: 6,476
Loc: Zone 5
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Quote:
the_chosen_one said: Speaking of strange colors. 
 Psilocybe Banderillensis day 11. Hard to see in that one but change the light frequency and back light, different story.
 Psilocybe Banderillensis day 11 2400K backlit.
This is pretty normal with Fagicola complex. I actually worry if they take off too fast and too white. Typically mold.
Yeah mine were very colorful, slow, and banded.
Weird creatures. Haven't figured them out yet. Anybody get these to fruit yet, or grow on grain for that matter?
I got them to take to LC, and the LC kept banked in the cold still leaps off on agar. My plates all sort of just stopped moving a few transfers out. Weird.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: Nichrome] 4
#28574668 - 12/08/23 04:43 PM (1 month, 19 days ago) |
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Similar here, neoxalapensis takes forever on grain.
Maybe a month since transfer:

I did try low-nute agar and I'm seeing this kind of growth.

About 8 days old transfer and it's jumping off fairly well. I cloned the blobs I was seeing above as well. They looked a good deal like pins.

Will update when I see what they do.
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scapo
Scientist



Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 48 minutes
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Re: Official Misc EXOTIC Thread [Re: Nichrome] 1
#28574684 - 12/08/23 04:55 PM (1 month, 19 days ago) |
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They colonized a pf cake nicely
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scapo
Scientist



Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 48 minutes
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Quote:
SupaThaRipper said: I have the same boxes 😄 do you store yours in the fridge? I’ve just been leaving mine out. I’ve heard it doesn’t matter either way.
I do! The original method stored their samples at room temperature. But I prefer the fridge.
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: Nichrome] 1
#28574699 - 12/08/23 05:09 PM (1 month, 19 days ago) |
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I've never seen anyone fruit them. Even in science research papers where you usually find them first. They are moody with stronger substrates.. at least the few species I've dealt with in the complex. Mine didn't like most grains but did ok on millet. I found the trick was to add vermiculite and weaken up the mix a bit. They can also be a little tricky getting to the final substrate. Secondary decomposers don't like that too rich either lol. I managed a decent colonization of hemp mat sandwiches with mixes of soil, vermiculite and WFP, but never got them to fruit. The hemp also works pretty well for Baeocystis for those working with those. Again, fruiting is another matter. Baeo's are touchy touchy and don't like anything we do to them. Indoors or out. This time around I'm testing LC on BRF or another grain, and agar, but I will be incorporating coir, hemp fiber and WFP into the PF Jars in an effort to duplicate rotting wood.
Hemp Bread
-------------------- "Luck favors the observant." - Workman
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
the_chosen_one said: I've never seen anyone fruit them. Even in science research papers where you usually find them first. They are moody with stronger substrates.. at least the few species I've dealt with in the complex. Mine didn't like most grains but did ok on millet. I found the trick was to add vermiculite and weaken up the mix a bit. They can also be a little tricky getting to the final substrate. Secondary decomposers don't like that too rich either lol. I managed a decent colonization of hemp mat sandwiches with mixes of soil, vermiculite and WFP, but never got them to fruit. The hemp also works pretty well for Baeocystis for those working with those. Again, fruiting is another matter. Baeo's are touchy touchy and don't like anything we do to them. Indoors or out. This time around I'm testing LC on BRF or another grain, and agar, but I will be incorporating coir, hemp fiber and WFP into the PF Jars in an effort to duplicate rotting wood.
Hemp Bread

Can you elaborate on hemp matt? Sandwiched between layers of nutrient poor sub?
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: wavyedge]
#28574731 - 12/08/23 05:44 PM (1 month, 19 days ago) |
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Yeah, I was using 4" square trays and putting a piece on the bottom. I'd gently mix the spawn into the weak sub in a baggie and then spread a thin layer of that onto the bottom piece then cover it with another piece. Around 1/4 to 3/8" thick. I'd recommend a clear or transluescent tray so you can see water build up. I made a mistake with black the first time. I also layer it with fine verm for protection while it colonized. The verm was then removed and replaced with cactus soil.. which may have been where the troubles were. I'm thinking that with maybe more sand this time. I may get some Pumice involved this time as well. Inski uses it with great success and I have had very little experience using it, so now might be the time.
-------------------- "Luck favors the observant." - Workman
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Thanks that's very helpful. My current plan to search for subs that baeos don't hate is to sterilize subs and use the little clear takeout cups people have been using.
When I found the baeos this season there was a lighter layer under the very dark top layer, I wonder if the landscapers put a light amount of straw or straw+manure followed by mulch.
Anyway, what your saying feeds some of what I was hypothesizing, very low nutrient or soil based, maybe with layers.
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28574847 - 12/08/23 07:16 PM (1 month, 19 days ago) |
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Great observations. I've also noticed they like loft. Probably why they are so hard to even transplant. Tried quite a few times back in the younger years. Every other wood lover is easy but Baeos are more like Semilanceata in this respect. I've seen them fill voids in PF cakes pretty thick. Try and spawn it though. Ugh. The only way I could even get them mostly colonized was using these and coir mats. They definitely need air through the whole process.
-------------------- "Luck favors the observant." - Workman
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Speaking of layers lol. Here's Zapotecorum Tapalpa moving onto the test sub. It looked a lot better.. BEFORE I DROPPED IT!
Yep it hit the floor good and hard. Fortunately I had the foil cover on. Now my nice flat layer is more of a wave. Think I'll call it the Derrumbe Tek. 
 Psilocybe Zapotecorum Tapalpa day 5 in substrate.
 Psilocybe Zapotecorum Tapalpa day 5 in substrate.
Oh! And I corrected my journal for the Hopii. It's actually Psilocybe affini Hopii.
-------------------- "Luck favors the observant." - Workman
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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On other fronts..
 Psilocybe Samuiensis T2 day 12 Yeah I get a little teary too lol. One more transfer and I think that particular one will be ready to test. Some of that can go to grain and LC though.
Forgot about this one.  Still need to start a journal entry. The wedge moved a little.
 Psilocybe Weraroa day 12
Hello aff. Hopii. About time you woke up.
 Psilocybe aff. Hopii day 12
-------------------- "Luck favors the observant." - Workman
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kirkeng
Stranger


Registered: 02/14/21
Posts: 805
Loc: Lost
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Been struggling to get good looking grain jars with papuana, but after trying rye and some new cuts I’ve finally got something that colonized relatively fast and consistently and looks healthy to me. Once these last couple tubs run their course I’ll be excited to give these guys another honest effort.

Hoping I can get to pin stage again as I really messed everything up last time and got a couple ideas of how I can improve this time. Time will tell.
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