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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 1
#28568444 - 12/04/23 03:03 PM (1 month, 23 days ago) |
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Quote:
kirkeng said: I’ve been enjoying them so far, I made the move towards them primarily cause I was having issues storing pans in the cold, but still fresh to them. Haven’t had any issues so far reviving things a year+ old. I’ll be curious to see how far they can be pushed though. Probably should be doing a secondary method as well though but space is too limited to really dig deep in that, maybe in the future…
I'm weighing work over space right now. 
Still pretty cool even if they only last a year. I love that ability to just grab a wedge with a pin drop and go. It's going to save a lot of time on this round. Probably in the future as well.
-------------------- "Luck favors the observant." - Workman
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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I have some notes to try glycerol (10-15℅) media, to find a concentration where myc will still grow. I had read a paper where they inoculated perlite with glycerol LC, grow from a small punch of mycelium and they were able to freeze (-80c) those cultures for preservation.
Now I haven't wanted to spring for the fridge, best I seem able to find that can hit -80c is ~3 grand.
But even if it works for 3-5 years in my freezer (-40c ish) maybe it would be worth having gone through the extra effort.
Found the paper
https://www.biomed.cas.cz/ccbas/Methods%20of%20Cryopreservation%20in%20Fungi.pdf
Edited by wavyedge (12/04/23 03:22 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 2
#28568471 - 12/04/23 03:35 PM (1 month, 23 days ago) |
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Quote:
kirkeng said: Very cool wavy, curious what you’ve learned so far with neoxalapensis, just streaked some plates the other day with those and caerulipes. Already getting some germination on the caerulipes and hoping the neo will follow suit, my last attempt with them I couldn’t get the print to germinate no matter how hard I tried… though I’m leaning towards trying that ATP agar chosen was talking about, eventually I’ll get around to that I suppose.
And I hope you do chosen, I can’t even fathom 50 species, I’m slowly working my way up but no where near that level of selections, haha.
Sorry, forgot to reply to you here,
So far, and it's been maybe two months, just that they seem to grow pretty slow, with MS producing some weird mycelium colors. I did a bunch of recent transfers to low nute grain agar and it seems to actually have improved. On grain it's very slow but I think I only put a few small wedges in. Going to LC or LI might get things moving.
Here are some T1 plates from earlier on in Nov, still moving but slow. Maybe a better transfer might yield faster growth too.

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moss
bryophyte


Registered: 10/27/12
Posts: 263
Loc: underfoot
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28568484 - 12/04/23 03:53 PM (1 month, 23 days ago) |
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28568587 - 12/04/23 05:23 PM (1 month, 23 days ago) |
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Quote:
wavyedge said:
Quote:
kirkeng said: Very cool wavy, curious what you’ve learned so far with neoxalapensis, just streaked some plates the other day with those and caerulipes. Already getting some germination on the caerulipes and hoping the neo will follow suit, my last attempt with them I couldn’t get the print to germinate no matter how hard I tried… though I’m leaning towards trying that ATP agar chosen was talking about, eventually I’ll get around to that I suppose.
And I hope you do chosen, I can’t even fathom 50 species, I’m slowly working my way up but no where near that level of selections, haha.
Sorry, forgot to reply to you here,
So far, and it's been maybe two months, just that they seem to grow pretty slow, with MS producing some weird mycelium colors. I did a bunch of recent transfers to low nute grain agar and it seems to actually have improved. On grain it's very slow but I think I only put a few small wedges in. Going to LC or LI might get things moving.
Here are some T1 plates from earlier on in Nov, still moving but slow. Maybe a better transfer might yield faster growth too.


That second one is beautiful. Reminds me of Hoogshagenii the last time I cultured it. Neoxalapensis is a new one for me. This is good information. Thanks!
-------------------- "Luck favors the observant." - Workman
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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 Psilocybe Aztecorum 1 of 3 LC to BRF day 12. Had to give it a Borg identification. 1 and 2 don't know where they came from source wise.  Pretty sure 3 does so I'm keeping it chronological until I find out.
 Psilocybe Niveotropicalis LC to BRF day 12.
Both seem to be enjoying the BRF just fine.
-------------------- "Luck favors the observant." - Workman
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
Loc: Castles made of sand
Last seen: 2 hours, 43 minutes
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Damn TCO,getting a nice library going again, all so very cool!
A question about transfers. It looks like you are taking small transfers during clean up on agar. Do you worry about limiting your genetic pool that way?
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: YoshiTrainer] 4
#28568835 - 12/04/23 07:56 PM (1 month, 23 days ago) |
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The smaller cubes are done with a punch so you can fit several in a cryo vial. I've been on the receiving end with those. I've been scalpeling out much larger peices here. Especially when working from MS. Some not so wedgey.  Not much of this is from MS though. At least right now. The current exceptions would be Samuiensis, Zapotecorum Valdivia and the pain in the ass Subtropicalis Valdivia which I won't post a pic of until I know it's not mold. lo..l? 
So there is a method to the chaos. I forgot to mention the PF jars serve kind of a triple purpose. They allow me to check incoming and domestic amplified LC for contaminants. If clean there's an opportunity to see if it can top fruit. Best to cover simple first. Otherwise extras make good spawn. I like to swap out grains/food sources too. The PF Tek is pretty adaptable in that respect. Just a great way to observe without a lot of waste.
Here's the rest of the current gang..
 Psilocybe Caerulescens SC LC to BRF day 29. Almost fully colonized. Probably another week to go.
 Psilocybe Caerulescens Tonila LC to BRF day 14. Been pushing this one hard and fast. Still can't believe it was alive.
 Psilocybe Zapotecorum Tapalpa LC to BRF/PB day 12.
 Psilocybe Zapotecorum Tapalpa LC to BRF day 12. This one is just flat out fast for a Zapo. The LC was corn soak water, corn syrup and vanilla. Same one I harvested from today. They sure seem to like it.
Making more jars later this week. Lots of testing to do.
-------------------- "Luck favors the observant." - Workman
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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I haven't looked at PF since I had bacterial contamination in my earliest of grows, but I do see a bunch of members putting it to good use (thinking fahtster and his pheno selection process). Might be worth finding some half pint jars again lol.
Just checking in on my neoxalapensis, it looks like they are liking the low nute grain agar I am making spawn dishes on:

And it almost looks like a primordia or a stone forming in this original T1 dish:

Thinking I should clone the blob.
Edited by wavyedge (12/06/23 09:11 AM)
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: wavyedge]
#28571326 - 12/06/23 10:06 AM (1 month, 21 days ago) |
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That's some pretty mycelium in that first pic. Looks like early Zapotecorum mycelium with that sweeping spiral outer edge. Anyone ever notice it's always a clockwise spiral inwards? At least in the northern hemisphere anyways. 
Yeah definitely clone that blob.

I didn't have enough plates to grab the pseudosclerotia from the Subtrop jar last round. I'm going to try and get it next round. Gotta hurry though. I saw some blue in it this morning. Hoping it makes it to the weekend.
-------------------- "Luck favors the observant." - Workman
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,488
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 3
#28571537 - 12/06/23 01:08 PM (1 month, 21 days ago) |
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Half pint jars are also perfect for micro centrifuges in the PC! So definitely worth having
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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What size are those and what are you using them for?
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Re: Official Misc EXOTIC Thread [Re: wavyedge] 1
#28571623 - 12/06/23 02:24 PM (1 month, 21 days ago) |
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I haven't checked the size I've been receiving from the trades, but they are all the same size regardless of who sends. Look about the same as those. I was planning on sterilizing the same way. Got so dang many I might not have to buy any for a while. 
Started journal entries on several species with more to come. Figured I'd better get on it now lol.
-------------------- "Luck favors the observant." - Workman
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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The 1.8 ml are the size I have been using. Seem pretty decent so far, have a little silicone gasket in the lid.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,042
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Quote:
the_chosen_one said: That's some pretty mycelium in that first pic. Looks like early Zapotecorum mycelium with that sweeping spiral outer edge. Anyone ever notice it's always a clockwise spiral inwards? At least in the northern hemisphere anyways. 
Yeah definitely clone that blob.

I didn't have enough plates to grab the pseudosclerotia from the Subtrop jar last round. I'm going to try and get it next round. Gotta hurry though. I saw some blue in it this morning. Hoping it makes it to the weekend.
I have absolutely noticed this. I thought I was crazy and that no one would believe me.

-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Not crazy..
-------------------- "Luck favors the observant." - Workman
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Land Trout
Stranger



Registered: 01/08/18
Posts: 3,076
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Quote:
elasticaltiger said:
Quote:
the_chosen_one said: That's some pretty mycelium in that first pic. Looks like early Zapotecorum mycelium with that sweeping spiral outer edge. Anyone ever notice it's always a clockwise spiral inwards? At least in the northern hemisphere anyways. 
Yeah definitely clone that blob.

I didn't have enough plates to grab the pseudosclerotia from the Subtrop jar last round. I'm going to try and get it next round. Gotta hurry though. I saw some blue in it this morning. Hoping it makes it to the weekend.
I have absolutely noticed this. I thought I was crazy and that no one would believe me.


One thing I notice with fine wispy tall cultures like blewit is they move when you mark the plastic above them or move your finger tip over them, some kinda dang ole electro magnetic fungal polarity man.
Unrelated to that. Here’s a subaeruginascens culture.
Edited by Land Trout (12/06/23 06:16 PM)
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SupaThaRipper
Genetics Hoarder



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Re: Official Misc EXOTIC Thread [Re: wavyedge] 2
#28571888 - 12/06/23 06:54 PM (1 month, 21 days ago) |
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1.8 marked but they’re technically 2ml. And for distilled water storage cultures (or whatever they’re called..lol)
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Mine are all 2ml. O ring in the lid.
T1's from Zapotecorum Valdivia are really cranking out some very clear sectors. A few not so good but several that are beautiful. Each plate is doing the same. There's going to be some real nice T2's with some variation. Too busy for pics atm but I'll get there lol.
-------------------- "Luck favors the observant." - Workman
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the_chosen_one
On the Darkslide


Registered: 09/11/06
Posts: 2,882
Loc: 1984
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Samuiensis T1 day 24. This is the one I put aside for observation. A little orangish around the wedge. Metabolites or bacteria maybe. Exotics can make some strange colors. Either way, I'm taking a little this weekend. Being able to see it in real 3D I gotta say.. 7:00 - 10:30



-------------------- "Luck favors the observant." - Workman
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