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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: thanks for fixing this Asura [Re: Mycoactive] 3
#26681909 - 05/19/20 08:53 PM (3 years, 8 months ago) |
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Had a fun grow this spring with Ps. subtropicalis (CaptainFuture's semperviva...). These things want to fruit so bad they were popping off the agar in days. Surprisingly high yield for a thin mushroom, though some specimens reach 9-10 inches in height.
Pasteurized straw pellets, nearly 1:1 spawn ratio (5 qt straw pasteurized at 170 degrees for 2 hours in a presto).
New favorite fungus.




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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
Celestialexplorer1 said: Just curious what kind of straw pellets did you use? By the way great work looks like it worked wonders.
Thanks, just straw pellets for rabbits (not hay), moistened to field capacity. I like to add a drop of dish soap and a teaspoon of CaOH (hydrated lime), then pasteurised. Then I cased with peat+verm.
https://www.amazon.ca/OXBOW-Bene-Terra-Eco-Straw-20-Pound/dp/B0017JGBAQ

Edited by wavyedge (05/21/20 11:29 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
alaskappalachian said:
 Highlight of my day by far was getting this in the mail Needed another species to feed to the slugs that exercise total dominion over the property Would be so ironic if after years of feeding woodlover spawn to my garden these were the first/only thing that ever fruited...
Glad it got there safe man! 
I have a head start, I managed to take clones of some of the fruits. They seem to grow decent (but slow) on soft PDA. This is first transfer after clone plate.

I found they cloned ok from the tissue under the pellicle, these have a thick one like semilanceata.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 3
#28568135 - 12/04/23 11:11 AM (1 month, 23 days ago) |
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I've had heimii on agar for weeks but the spores in the swab were probably dead. Cool to hear you've got that one going! I've been working on neoxalapensis for a while, it's a fair bit slower than say semps.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
the_chosen_one said:
Ok I'm back. Was racing the cooling agar.
So how are you liking the water vials? Many of the trades I've been getting are in them. I've never tried them but thought about it a bit. I've been backing up with prints when I get that far. Slants in the middle stages after I settle on a selection. Plates and LC in capped syringes for the early stages. The vials definitely make shipping easier but I wonder how long they can last. I would imagine about the same as a capped syringe of LC which depending on species can last several years. Hmmm. I guess with a little maintenance now and then they could replace slants. Man that'd sure be nice. Maybe I'll go that route.
You mean these?

I just got set up with them and haven't had much experience, yet.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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I have some notes to try glycerol (10-15℅) media, to find a concentration where myc will still grow. I had read a paper where they inoculated perlite with glycerol LC, grow from a small punch of mycelium and they were able to freeze (-80c) those cultures for preservation.
Now I haven't wanted to spring for the fridge, best I seem able to find that can hit -80c is ~3 grand.
But even if it works for 3-5 years in my freezer (-40c ish) maybe it would be worth having gone through the extra effort.
Found the paper
https://www.biomed.cas.cz/ccbas/Methods%20of%20Cryopreservation%20in%20Fungi.pdf
Edited by wavyedge (12/04/23 03:22 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 2
#28568471 - 12/04/23 03:35 PM (1 month, 23 days ago) |
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Quote:
kirkeng said: Very cool wavy, curious what you’ve learned so far with neoxalapensis, just streaked some plates the other day with those and caerulipes. Already getting some germination on the caerulipes and hoping the neo will follow suit, my last attempt with them I couldn’t get the print to germinate no matter how hard I tried… though I’m leaning towards trying that ATP agar chosen was talking about, eventually I’ll get around to that I suppose.
And I hope you do chosen, I can’t even fathom 50 species, I’m slowly working my way up but no where near that level of selections, haha.
Sorry, forgot to reply to you here,
So far, and it's been maybe two months, just that they seem to grow pretty slow, with MS producing some weird mycelium colors. I did a bunch of recent transfers to low nute grain agar and it seems to actually have improved. On grain it's very slow but I think I only put a few small wedges in. Going to LC or LI might get things moving.
Here are some T1 plates from earlier on in Nov, still moving but slow. Maybe a better transfer might yield faster growth too.

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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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I haven't looked at PF since I had bacterial contamination in my earliest of grows, but I do see a bunch of members putting it to good use (thinking fahtster and his pheno selection process). Might be worth finding some half pint jars again lol.
Just checking in on my neoxalapensis, it looks like they are liking the low nute grain agar I am making spawn dishes on:

And it almost looks like a primordia or a stone forming in this original T1 dish:

Thinking I should clone the blob.
Edited by wavyedge (12/06/23 09:11 AM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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What size are those and what are you using them for?
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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The 1.8 ml are the size I have been using. Seem pretty decent so far, have a little silicone gasket in the lid.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: YoshiTrainer] 1
#28574085 - 12/08/23 08:52 AM (1 month, 19 days ago) |
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Nice, thx for the link. Been buying the bags of 2ml bottles but a box is nice to have for nearly the same price.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: Nichrome] 4
#28574668 - 12/08/23 04:43 PM (1 month, 19 days ago) |
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Similar here, neoxalapensis takes forever on grain.
Maybe a month since transfer:

I did try low-nute agar and I'm seeing this kind of growth.

About 8 days old transfer and it's jumping off fairly well. I cloned the blobs I was seeing above as well. They looked a good deal like pins.

Will update when I see what they do.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
the_chosen_one said: I've never seen anyone fruit them. Even in science research papers where you usually find them first. They are moody with stronger substrates.. at least the few species I've dealt with in the complex. Mine didn't like most grains but did ok on millet. I found the trick was to add vermiculite and weaken up the mix a bit. They can also be a little tricky getting to the final substrate. Secondary decomposers don't like that too rich either lol. I managed a decent colonization of hemp mat sandwiches with mixes of soil, vermiculite and WFP, but never got them to fruit. The hemp also works pretty well for Baeocystis for those working with those. Again, fruiting is another matter. Baeo's are touchy touchy and don't like anything we do to them. Indoors or out. This time around I'm testing LC on BRF or another grain, and agar, but I will be incorporating coir, hemp fiber and WFP into the PF Jars in an effort to duplicate rotting wood.
Hemp Bread

Can you elaborate on hemp matt? Sandwiched between layers of nutrient poor sub?
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Thanks that's very helpful. My current plan to search for subs that baeos don't hate is to sterilize subs and use the little clear takeout cups people have been using.
When I found the baeos this season there was a lighter layer under the very dark top layer, I wonder if the landscapers put a light amount of straw or straw+manure followed by mulch.
Anyway, what your saying feeds some of what I was hypothesizing, very low nutrient or soil based, maybe with layers.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: kirkeng] 1
#28575143 - 12/08/23 11:02 PM (1 month, 19 days ago) |
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Quote:
kirkeng said: Been struggling to get good looking grain jars with papuana, but after trying rye and some new cuts I’ve finally got something that colonized relatively fast and consistently and looks healthy to me. Once these last couple tubs run their course I’ll be excited to give these guys another honest effort.

Hoping I can get to pin stage again as I really messed everything up last time and got a couple ideas of how I can improve this time. Time will tell.
Nice, I was thinking of giving rye a shot with another species.
I'm following along with two strains of Ps. papuana I'm really excited to get to grain. Keep updating about this one, I've been fascinated since Heyowana's finds.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: Adas] 3
#28575913 - 12/09/23 02:14 PM (1 month, 18 days ago) |
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New Ps papuana thread spinning off for those who may have missed it: https://www.shroomery.org/forums/showflat.php/Number/28575437
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: scapo]
#28576730 - 12/10/23 08:34 AM (1 month, 17 days ago) |
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Quote:
scapo said: Thanks for sharing! I have a pf cake colonized I’m about to get rockin with; i’ll post soon
My plan for the caeruleoannulata is to treat it like I did the stuntzii! Chip spawn to pots and totes, cased and cooled
Scapo, would you mind giving more detail on your fruiting chamber that you used for stuntzii? I think you did fruited ovoids too, correct?
I'm looking at trying to fruit ovoids in a modified fridge. Did you track conditions with a logger or did you happen on what conditions worked?
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: Official Misc EXOTIC Thread [Re: scapo]
#28576806 - 12/10/23 09:43 AM (1 month, 17 days ago) |
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Quote:
scapo said: For sure!
The ovoids were easy: I took some colonized chips, cased them in a shoebox, and covered them and 4weeks later they fruited. No chamber!
The stuntzii initially fruited indoors in a wine fridge. In a 64oz tupperware container with the lid on. It took a bit of time for them to pin but the pinset was solid in the fridge.
Once I saw pins I put them into the chamber. The chamber is a large tote with fae holes with seed lights affixed inside and an aquarium air pump that has the lines fed into jars of water. This is dual purpose: The constant positive pressure prevents contam getting in, and the air stays humid because the pump is bubbling through water.
Cool thanks! Reading through mushpuppet's grow from years ago, ovoids pinned for him at room temperature. I think they thought they were caerulipes at the time.
Bobwastaken also used an air pump to push in air like what you described. Interesting to note that both the ovoid and stuntzii seemed to continue fruiting once pinning started despite being moved to warmer conditions.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
the_chosen_one said: Looks pretty normal to me..
Yeah you have to give Stuntzii a little cold bump and then they will fruit indoors at temps between 55f and the upper 60s. I have pics in a broken computer that I did in my basement along side of some Subaeruginascens a few years ago. No air pump. Just a tub with frequent airing and misting.
One of these days I'll fix it lol. Got a lot of pics and some valuable stuff in there. So when I hit the power button it starts the fans and hard drive spinning at high speed and won't go farther than that. Anyone know what that could be? I got lucky once and it started but the screen went black before I could finish downloading pics.  Trying again now and all I hear is fans.
Sounds like your windows install or motherboard. Regardless probably not your drive. Take the drive out and plug it into a USB enclosure, plug that into a working PC. If you take the drive to best buy or staples and ask for usb enclosure they have a decent chance to point you at the part you need. Probably just get this
https://www.amazon.com/Adapter-FIDECO-Drive-Cable-Universal/dp/B0919N4XNW/ref=mp_s_a_1_12?crid=3G4PADAMNLGU8&keywords=sata+ide+USB+with+power&qid=1702257437&sprefix=sata+ide+usb+with+power%2Caps%2C184&sr=8-12
It will handle any drive from the last 20yrs
Browse files, find files, copy to working PC. Can also just plug it in as a secondary drive to a tower, same process.
Edited by wavyedge (12/10/23 06:21 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Quote:
SupaThaRipper said: You’re the man wavy! Because I have a stack of pcs I need to go through 😂 what’s worse is I started taking the fans out of them and other things to start using for other various projects 😂 so it’s nice to know you can just plug the hard drive into something else
I don't want to go too far off-topic but you'll get charged $200+ by staples to do that work. IMO if you already bought another computer and all you need to do is get at files then don't get gouged.
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