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Invisibletryptonite
mushroom ninja
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Registered: 02/06/04
Posts: 808
Loc: aussie
innoculating with prints
    #2586304 - 04/21/04 10:54 AM (13 years, 1 month ago)

is there any possible way to innoculate jars without using syringes? I innoculated 2 pf jars using a loop and sporeprint. will this work?


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: innoculating with prints [Re: tryptonite]
    #2586353 - 04/21/04 11:17 AM (13 years, 1 month ago)

It can be done...  You want to watch the sterile procedures, though...


For starters, you have to crack the lid to do it (rather than just squirting through a hole that just needs the tape lifted for 3 seconds...) 
For seconds, putting the spores in liquid (in the syringe) rehydrates them beforehand and speeds up germination...

You would do well to make a glovebox (or at least research 'oven tek')

I'm assuming you're having probs sourcing syringes...  (If you're considering this as a 'labour saving initiative', you're on the wrong track :wink: )


There are probably a few more reasons why syringes are better that I can't think of right now...  (Never fear...  Someone smart will come along soon :smile: )


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OfflineThatDaltonGuy
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Registered: 04/07/04
Posts: 213
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Last seen: 13 years, 6 days
Re: innoculating with prints [Re: tryptonite]
    #2586455 - 04/21/04 12:05 PM (13 years, 1 month ago)

if you need syringes find one of those like "planned parenthood" or "HIV testing" places near you and tell them your a heroin addict and u need clean needles and they donate like 10 to you... thats what i did =)

they are only 1CC syringes and they work for like 1 jar... so u can make tons of them if you choose... but anyways its better than a print =)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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