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Azurescen will grow on Pine * 1
    #25860817 -
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These are pictures of Azurescen mycelium on Pine shavings.
The first is at 7 days.
The second is at 15 days.
The third is at 20 days.
Has any one else grown wood lovers on softwood and how did you do it?
I have uploaded them hope I have done it right.

Edited by Growman (03/08/19 12:30 PM)

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Re: Azurescen will grow on Pine [Re: Growman]
    #25860842 -

Nope.


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Re: Azurescen will grow on Pine [Re: Mr. Funguy]
    #25860945 -

No but I would love to learn!
Care to help me? Gratz that's looking awesome!

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Re: Azurescen will grow on Pine [Re: Anoobus]
    #25860958 -

Wow what did you inoculate the jars with?

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Re: Azurescen will grow on Pine [Re: Comfortablepants]
    #25861065 -

Badass!


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Re: Azurescen will grow on Pine [Re: Anoobus]
    #25861173 -

Do you have a clean wood lover spore syringe?

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Re: Azurescen will grow on Pine [Re: Comfortablepants]
    #25861184 -

Do you want to know how I made my grain spawn?

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Re: Azurescen will grow on Pine [Re: Growman] * 3
    #25861281 -

I wana know why you uploaded a zip instead of pics lol

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Re: Azurescen will grow on Pine [Re: Growman]
    #25861342 -

For my first experiment with growing on pine shavings I inoculated three jars with rye spawn.
The first was on Pine shavings soaked in water for three days. Any of the Rye that was not fully colonized and touched a wood shaving contaminated to green.

The second jar was pasteurized. It did colonize very slowly but eventual contaminated with lip stick mold I think through the filter.

The therd jar was sterilized. I could see mycelium white puffs pressed against wood shavings but did not colonize for many weeks. Eventual some of the thinner shavings did take and once established ripped through the jar.

So I new that Azurescen would grow on Pine but I had to find a better way to innoc.

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Re: Azurescen will grow on Pine [Re: bodhisatta]
    #25861353 -

Because I am far better at biology than technology. I uploaded them but could not get them to post and was afraid that may be I had posted them ten times.

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Re: Azurescen will grow on Pine [Re: Comfortablepants]
    #25862088 -

In a tek on Peroxide use there was a paragraph on selectivity. The article enplaned that mushrooms have the genetics to produce many different enzymes that can digest many different things. When a mushroom grows well on Alder that is because that gene is turned on. In the background there are genes to produce enzymes that can digest many other wood types but they are not expresses because the mushroom dose not need them, it is growing on Alder.

The back ground genes do not switch on quickly that is why in the Peroxide tek they start with Peroxide in the Agar.

I made rye spawn with Pine shavings to get the mycelium used to digesting Pine. I made spawn jars with different concentrations of shavings to see which one would colonize the shaving jars the fastest.

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Re: Azurescen will grow on Pine [Re: Growman]
    #25862109 -

Growman said:
I made rye spawn with Pine shavings to get the mycelium used to digesting Pine. I made spawn jars with different concentrations of shavings to see which one would colonize the shaving jars the fastest.



Very interesting, would like to know if that's true. What were your results?


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Re: Azurescen will grow on Pine [Re: Lyh]
    #25862116 -

Lyh said:
Growman said:
I made rye spawn with Pine shavings to get the mycelium used to digesting Pine. I made spawn jars with different concentrations of shavings to see which one would colonize the shaving jars the fastest.



Very interesting, would like to know if that's true. What were your results?




Indeed, pictures for how much including info.


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Re: Azurescen will grow on Pine [Re: Lyh]
    #25862135 -

I came to a spawn mix that will colonize a 2 quart jar of pine shavings in about 20 days.

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Re: Azurescen will grow on Pine [Re: Growman] * 1
    #25862286 -

Ive grown ps cyans on pine chips before. Ive also picked cyans off pine chips.

Its for realz.

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Re: Azurescen will grow on Pine [Re: LogicaL Chaos] * 1
    #25862731 -

Wood lovers adapt well to many coniferous species.

About the only species I've seen them refuse to colonize is wester red cedar.

Our friends down in aus find them in the pines all the time.


:shrug:


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Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Azurescen will grow on Pine [Re: Mr Piggy] * 3
    #25862759 -

The idea that you need to "get them used to" the substrate is some old paul stamets hooey bullshit. Gene expression and up regulation happens at the speed of cellular dynamics which is fast as shit. They're already expressing the genes for degradation of all kinds of things. They'll upregulate gene expression they need in real time. This isn't something that takes more than minutes/hours.

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Re: Azurescen will grow on Pine [Re: bodhisatta]
    #25862762 -

Interesting.

:takingnotes:!

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Re: Azurescen will grow on Pine [Re: bodhisatta]
    #25862771 -

it's like trying the "this spoon is an airplane" trick with a fat kid.

It was never necessary.

I'd know, I was that fat toddler.


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🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Azurescen will grow on Pine [Re: LogicaL Chaos] * 1
    #25862772 -

There are lots of studies on it by actual scientists and mycologists

Quote:
IMPORTANCE All species of wood-decaying fungi occur on a characteristic range of substrates (host plants), which may be broad or narrow. Understanding the mechanisms that enable fungi to grow on particular substrates is important for both fungal ecology and applied uses of different feedstocks in industrial processes. We grew the wood-decaying polypore Fomitopsis pinicola on three different wood species, aspen, pine, and spruce, under various culture conditions. We examined both gene expression (transcription levels) and RNA editing (posttranscriptional modification of RNA, which can potentially yield different proteins from the same gene). We found that F. pinicola is able to modify both gene expression and RNA editing profiles across different substrate species and culture conditions. Many of the genes involved encode enzymes with known or predicted functions in wood decay. This work provides clues to how wood-decaying fungi may adjust their arsenal of decay enzymes to accommodate different host substrates.




It's long been known that pretty much any organism will change gene expression to match substrate (or food)

In fungi this doesn't take long at all. As soon as the lag phase from being transferred is over cellular receptor proteins create feedback loops and genes get preferentially expressed to degrade the given substrate most effectively. You don't need to precondition fungi to degrade a certain substrate. They either have the genes or they don't. They'll upregulate the needed genes in real time if you say put azures on conifer wood.

Gene transcription and translation happens ridiculously fast at a cellular level

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Re: Azurescen will grow on Pine [Re: LogicaL Chaos]
    #25862851 -

I was pleased to hear from. I encourage others to come forward with there grows on non traditional wood and how they did it.

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Re: Azurescen will grow on Pine [Re: Growman]
    #25862858 -

It's only non-traditional to you.

Those of us int he PNW that have been dealing with wood lovers for a while grow them on conifers all the time and find them growing on conifers.

Not trying to be rude, just consider the words you use.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Azurescen will grow on Pine [Re: Mr Piggy]
    #25862875 -

I think you are right but I have seen many people say I can not get Alder what else can I use and the reply is get Alder.

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Re: Azurescen will grow on Pine [Re: Mr Piggy]
    #25862887 -

I would say its unusual to cultivate it on pine. Most people cultivate wood lovers on alder chips.

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Re: Azurescen will grow on Pine [Re: Mr Piggy]
    #25862889 -

What word would you use, may be nonrecommended?

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Re: Azurescen will grow on Pine [Re: LogicaL Chaos]
    #25862896 -

It's like if you ask how to make pasta people say you need pasta. Wood lovers like hardwood. No ones going to be telling you go grab pine cones and conifer chips even if they can and do work. It's just not where you start if you have better options.

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Re: Azurescen will grow on Pine [Re: bodhisatta]
    #25862898 -

Is Alder preferred because it has more nutrients perhaps?

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Re: Azurescen will grow on Pine [Re: LogicaL Chaos]
    #25862902 -

I would say sap, ph, inhibitory compounds, wood density, all play a roll as well as more usable nutrition for fungi to derive from hardwood

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Re: Azurescen will grow on Pine [Re: bodhisatta]
    #25862910 -

You may be right. You obviously have a deeper understanding of this than I do. Mine is an informal study but the shaving mixed grain colonized the jars faster than straight grain.

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Re: Azurescen will grow on Pine [Re: Growman] * 1
    #25862930 -

It's easier to narrow down what they don't like.  Sure, you'll get a huge awesome flush on alder, and that's usually it.  They can eat through alder so fast it's mind boggling.  I feel like I have to rechip those patches twice a year (exaggeration for sure)

Personally I have spawned patched to Douglas-Fir and had great success.  I put a patch on my friend's farm in DF a couple of years ago and it's still going strong... when he remembers to water them. 

I've noticed cyans really take well to DF cones and you can make spawn bombs with them.  Here a cone, there a cone, everywhere a spawn cone.

Optimally I would spawn them to a mix of oak and alder, or maple and alder.

Really whatever you have on hand in a temperate environment besides cedar should do.


--------------------
🅃🄴🄰🄼 🄸🄷🄾🄿


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: Azurescen will grow on Pine [Re: Mr Piggy]
    #25863906 -

Now I am not sure how to explain why the grain with shavings colonizes the substrate faster may be its as simple as it makes better contact.

The consensus is Alder is the best. Has any body done side by side tests to rate say the top twenty?

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Re: Azurescen will grow on Pine [Re: bodhisatta]
    #25866302 -

There is not much that can be done about sap or wood density but PH and nutrition could be adjusted during the soak. Has any work been done on improving the less recommended woods?

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Re: Azurescen will grow on Pine [Re: Mr Piggy]
    #25866352 -

"I've noticed cyans really take well to DF cones and you can make spawn bombs with them.  Here a cone, there a cone, everywhere a spawn cone."

Mr Piggy
Did you just bury the cones in a bed then dig them up or do you have a tek?

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