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Amazon Shop: Autoclave

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OfflineFungusBoi
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Registered: 10/03/01
Posts: 59
Last seen: 8 years, 4 months
autoclave + uber bulk
    #2585946 - 04/21/04 05:05 AM (13 years, 6 months ago)

hey guys n gals. i havn't posted or checked out the shroomery in like a year now, i'm a bit rusty on all my teks and whatnot. i recently found out that a good friend who works in a lab around me will let me use his industrial autoclave. it gets up to around 250 degrees, and i can sterilize anything up to the size of a garbage can in 40 minutes, including drying time :-)

i presume autoclaving is the best way to sterilize, are there any complications i'm not foreseeing?

anyone have an idea how i can innoculate a garbage bag full of substrate? i'm trying to figure out how to get enough spores...maybe print every mushy that fruits off of a single mycellial structure?

i'm also trying to figure out the best way to make this project absolutly ridiculous, probably going to construct a refridgerator type storage unit. any ideas there?


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"Never rest for even a second, because a second becomes a minute, a minute becomes an hour, a hour becomes a day, and a day, a lifetime" -PTEI


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InvisibleJoshua
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Registered: 10/27/98
Posts: 5,389
Loc: The Matrix
Re: autoclave + uber bulk [Re: FungusBoi]
    #2585965 - 04/21/04 05:22 AM (13 years, 6 months ago)

5 gallon buckets can be used for large quantities of substrate. The plastic is made of HDPE 2. The lids tend to warp a bit, but who cares when you consider the use you can get out of the pair. Garbage cans can also be used if they are made of the right plastic. I have heard that PP 5 can be used as well.

Here is a great source of info: http://www.brand.de/english/techinfo/plastic/plast2_e.htm

I always do a sample run first to determine if the plastic I want to use can be PC'd or autoclaved.

Good luck!!

250F = 121 C

Joshua


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Amazon Shop: Autoclave

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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