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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery] * 1
    #25838078 - 02/25/19 11:42 PM (5 years, 1 month ago)

Liquid innoculant & liquid culture


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InvisibleFiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25838083 - 02/25/19 11:45 PM (5 years, 1 month ago)

Quote:

Noisecontrol said:
Liquid innoculant & liquid culture




:fuckyeah:


So, what's the difference between the two? Is LI spores and LC live mycelium?

If I had an agar plate, how would I go about getting it ready for a LC? You can't just put the agar plate into a blender and then suck it up with a syringe? That sounds like a contamination nightmare.

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
    #25838099 - 02/26/19 12:12 AM (5 years, 1 month ago)

Not all people with a moustache are a pedophile, but all pedophiles have moustaches.

:feelsgoatman: i keed

LI can be live culture or spores, liquid culture is the already germinated organism.

You would establish clean myc on a plate and xfer that to a liquid nutrient suspension. Many use tools for agitating the culture once its growing in the suspension such as magnetic stir bars. This replicates shaking grain jars, only you do it much more often and typically over a long period of time.


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InvisibleFiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25838119 - 02/26/19 01:00 AM (5 years, 1 month ago)

:orly:


Well that makes sense then. I am learning a lot.

:bumpthread:

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
    #25838130 - 02/26/19 01:10 AM (5 years, 1 month ago)

I like the method i mentioned because i hate waiting on LC to grow and it is a gamble if you're not certain of the cleanliness for whatever reason. Its a lot more convenient to me to extract some mycelial fibers from breaking spawn jars before i throw them to bulk. Literally all i have to do prep wise is keep a pc'd jar of sterile water and sterile syringes, and i can make LC of whatever variety i have a grain jar of. Technically, if you have a completely colonized plate, you could prep some nutrient suspension, get it sterile, and extract a syringe of it to then agitate the mycelium on the plate, squirt the syringe worth of nutrient suspension on it and suck it back up. This way you keep a jar of the nutrient and can make varieties of LC in individual syringes. I like this way if I have a bunch of varieties or clones i want to make LC from.


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InvisibleFiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25838132 - 02/26/19 01:13 AM (5 years, 1 month ago)

With oysters you can literally take rolls of toilet paper, soak them with boiling water, throw a little bit of spawn on them, tie them in a grocery bag, and just fruit them in a few weeks, or break them up into giant bins of "cold pasteurized" wheat straw.


wow. SO much easier! and so tasty.


But the hard work of cloning is worth it to grow more rare species!

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OfflineThesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
    #25838211 - 02/26/19 03:37 AM (5 years, 1 month ago)

Quote:

Fiery said:
Quote:

Noisecontrol said:
Liquid innoculant & liquid culture




:fuckyeah:


So, what's the difference between the two? Is LI spores and LC live mycelium?

If I had an agar plate, how would I go about getting it ready for a LC? You can't just put the agar plate into a blender and then suck it up with a syringe? That sounds like a contamination nightmare.



LI is just a wedge of agar diluted in water while lc is actualy growing the mycelium. I prefer my method because you don’t need to wait for it to grow/breed possible contamination and you don’t need any nutrients. If you leave LI sitting around it will turn into lc I guess, just wouldn’t wan t to leave it sitting because there is no gas exchange. Used fresh is best anyways.
I use a jar with just an injection port and a little more water than what can fit in my syringe. Drop the agar in and give it a shake, then suck it though the injection port and use the syringe.

Edited by Thesunbeam (02/26/19 03:41 AM)

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25838652 - 02/26/19 09:14 AM (5 years, 1 month ago)

Time plays no part in the cleanliness. If it's gonna contaminate, that will be the outcome regardless. All of these processes require sterile technique. Your agar wedge in plain water has the same odds of contam as a nutrient suspension.


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OfflineHoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25848865 - 03/02/19 11:05 PM (5 years, 27 days ago)

UPDATE. Original batch of jars were just shaken up all were about 25 percent finished.
So far so good. They all looked clean.
Oyster myc is also a lot faster than cube.
Just hoping my clone performs good. It was a crappy flush one pint brf crumbled to coir.  but had a giant fruit in there.
Exciting stuff.


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when god hands you lemons you,FIND A NEW GOD! 

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Offlinepoisoned
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25849296 - 03/03/19 08:05 AM (5 years, 26 days ago)

according to my understanding, LC media offers way better conditions for contaminants to grow than grain or agar. Please correct me if I'm wrong.


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Edited by poisoned (03/03/19 08:05 AM)

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: poisoned]
    #25849348 - 03/03/19 08:43 AM (5 years, 26 days ago)

Theyre not comparable. Theres tons of different contam variables to each. Cleanliness is universal to all of them. Its a common misconception that your grain jars contam less than your culture mediums. In fact they probably contam more due to endospore rate, mostly being bacterial that you only see as an outcome of mycelial growth pattern, and ultimately short life of your fruiting substrate. If you're contamming at all, you should practice better sterile technique, not just attempt a different material.


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OfflineHoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25852757 - 03/04/19 06:31 PM (5 years, 25 days ago)

uPdate. It’s been over 24 hours since I shook them. All look great no contamination yet. Hella excited
Anyways here is a photo of one of my jars I shook and also the oyster  clone loving the oats.

I know it’s lame updating small shit like this. But I like having a somewhere to record my progress.


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InvisibleFiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25852869 - 03/04/19 07:22 PM (5 years, 25 days ago)

Quote:

Hoob33 said:
uPdate. It’s been over 24 hours since I shook them. All look great no contamination yet. Hella excited
Anyways here is a photo of one of my jars I shook and also the oyster  clone loving the oats.

I know it’s lame updating small shit like this. But I like having a somewhere to record my progress.




I've been watching your progress. It's not lame..


SO EXCITED!!

WOO!!!:fuckyeah:

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OfflineHoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
    #25858675 - 03/07/19 03:07 PM (5 years, 22 days ago)

It’s been only 3 days.  Here is the same jars.
The other aa’s  are a little behind.this one here I did a tiger drop.
Super excited or these oysters tho. Just got some SOfT wood pellets for them. All I could find.
Gonna do lions mane and shiitake once i get a hold of some cultures.



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when god hands you lemons you,FIND A NEW GOD! 

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OfflineEnDelfisE
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25858789 - 03/07/19 03:56 PM (5 years, 22 days ago)


Quote:

Hoob33 said:
They could have been way too wet.
I used to drink When I did a lot of mycology
Thankfully it’s now a thing of the passed
Haven’t got any wet filters this time.
I just remember them feeling wet when all my jars got messed up.




wet filters have ruined some lcs of mine too before I realize simple tyvek glued with silicon it can get wet and actually I saw some steam coming up from the still warm jar( not hot ) and coming out of the pores of the thin tyvek as microscopic water droplets, I thought just because I tilted the jar filled with water upside-down and tyvek remained dry with no leaks that it was waterproof... little did I know ... after pcing I guess something happened and the pores expanded somehow , anyway these lcs half became bacterial

I tried to find sfds or watmans filters I even tried to build a weird frankenstain lid yeah thats how paranoid I got :rolleyes: 



but I think what helped me the most was getting a cheap " hard " tyvek sheet from a craft store rated at 105g/m² while soft was rated at 40 almost 3 times less , so I did some experimentation with that and it seems like its too thick for water to pass through not even when after pcing , I cut it in small round pieces and m3 micropore tape it in my gas exchange hole which is pretty big holes since I thought the more dense the tyvek the harder it would breath , seems to be serving me well after lots of jars and lcs , I haven't had any contams with it yet , now im checking to see how reusable is it, it doesnt seem to degrade after 4-5 pc cycles , I walso pc 3 hours at 11-12 pci I think cuz my European pc sux :laugh: different standards here as it seems , damn I miss my presto when lived in the us! so cheap and so useful ! amazon even replayed the first one I got and destroyed on the first run... running out of water :S I told them it became inflated and round like an egg ready to explode while normal pressure cooking! next day the send me a new one! I love America <3

Edited by EnDelfisE (03/07/19 04:10 PM)

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OfflineEnDelfisE
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25858814 - 03/07/19 04:08 PM (5 years, 22 days ago)

Quote:

Hoob33 said:
uPdate. It’s been over 24 hours since I shook them. All look great no contamination yet. Hella excited
Anyways here is a photo of one of my jars I shook and also the oyster  clone loving the oats.

I know it’s lame updating small shit like this. But I like having a somewhere to record my progress.





damn that was fast!!! I remember my first time ever getting into growing mushrooms , before I discover this meca of knowledge mushroom <3
I got a spore syringe from a vendor called spores101 if I remember correctly ... I was so excited! I remember looking to verm in my small hometown ... garden stores didnt even know what it was back then haha
finally found some! started watching rrs videos all day! everything seemed to be going great! and after a few days of birthing the cakes... oyster mushrooms apeard on them... I was freaking out! https://www.shroomery.org/forums/showflat.php/Number/16996445/page/2

the funny thing is cubies made their appearance too a few days after on the same cakes with the oysters! I cant complain tho, oysters made a delicious meal!

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OfflineHoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: EnDelfisE]
    #25866306 - 03/10/19 06:16 PM (5 years, 19 days ago)

UPDATE. here’s my AA+ and oyster clone jars.
I think they are about ready to rock. What do you think?
My other jars are still recovering. I have them in a tote and my house has been in the 50-60F.
These two jars were stored in a higher/warmer spot. So they finished up faster.



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when god hands you lemons you,FIND A NEW GOD! 

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OfflineJoeyddr
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25866419 - 03/10/19 07:30 PM (5 years, 19 days ago)

Quote:

Hoob33 said:
UPDATE. here’s my AA+ and oyster clone jars.
I think they are about ready to rock. What do you think?
My other jars are still recovering. I have them in a tote and my house has been in the 50-60F.
These two jars were stored in a higher/warmer spot. So they finished up faster.






first pic looks blurry but the second looks pretty clean to me.


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OfflineHoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Joeyddr]
    #25868904 - 03/12/19 01:33 AM (5 years, 18 days ago)

So today i spawned both these jars. I did the cubes with cvg used a 1-1.5 ratio.
And I did the oyster with a mix of fir pellets,a keystone cardboard box I shreddd ,
And the rest of the cvg I had laying around. I tied a garbage bag around the basket.
Gonna fruit them in my laundry room. Seems like more fresh air cirrculates in there.
I smelled the oyster jar. I thought I fucked it up because it smelled so sweet And aromatic.
Haha.


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when god hands you lemons you,FIND A NEW GOD! 

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25870559 - 03/12/19 08:31 PM (5 years, 17 days ago)

What was your oyster ratio?


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