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Hoob33
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Registered: 02/25/17
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2nd ever agar attempt.Grow log. Pics.
#25835512 - 02/24/19 08:39 PM (5 years, 10 months ago) |
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Yo. Last year I went ahead and tried doing some agar to grain. HUGE FAIL. every jar I made contaminated. I gave up on agar and went back to ms syringe. What a gamble /shit show. New Years resolution is to get it right this time. I said SCREW WBS. went out finally found some oats. Made two batches. One was made following bods easy oat tek. Another batch I made was made following the RR dvd method. “24 hour soak “ went ahead and made a shit load of pasty plates and went though them real quick. Made transfers,cloned the biggest fruit from an old ms grow. And germinated spores ects...my contamination rate has significantly gone done the more i practice sterile technique.
So here I am today with several clean cultures I’ve expanded into several other plates. Just dropped some wedges in my 24 hour soak grain and it’s been a week since I dropped some wedges in the first batch. Also I made a few different lids. “Multi spore .tyvek .unmodded “ whatever works for me is what I’m sticking to. Here is some photos
One of several agar plates

Different style lids. I’m hoping this tote will keep them safe.

First batch wedges dropped

One week later Aa+ germinated from spore t-8 Golden teacher clone t 6

Wild oyster clone taken from this cluster sorry for the blurry ness it’s pulmonarius

 Here is a t-7 wedge I did last week of it.
 Here is the A2g I made today using 24 hour soaked grain.

I noticed doing 24 hour soak and a longer drying period the grains came out prefect. The first batch had to be shaken to even out some moisture but it looks amazing compared to messy ass bird seeds Anyways. This is my second attempt I don’t wanna give up I love this hobby and any advise and input are more than welcome. Also I have a few t-3 pan cyans on grain. Haha I said I wasn’t gonna do to many projects on agar. Keep it simple but it’s hard when theirs so many possibilities.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
Edited by Hoob33 (02/24/19 08:42 PM)
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25835782 - 02/24/19 10:40 PM (5 years, 10 months ago) |
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25835800 - 02/24/19 10:46 PM (5 years, 10 months ago) |
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I highly recommended agar LI. You can turn one wedge of agar into a few syringes that are easier to manage and more successful than transferring an agar wedge to each jar.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25835806 - 02/24/19 10:50 PM (5 years, 10 months ago) |
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I was thinking making LC But I don’t wanna introduce another source of contamination into the equation.if you know what I mean. Maybe in a few weeks once I get this down a little more. The speedy colonization of a LI is titalating haha.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25835832 - 02/24/19 11:05 PM (5 years, 10 months ago) |
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I like LI so much because unlike making multiple transfers to jars where you might pick up a contam in between jars, you only transfer to one jar and then the culture is contained within the LI jar and the syringe.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25835838 - 02/24/19 11:10 PM (5 years, 10 months ago) |
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I will say that LI is a real benefit to saveing time while innoculating large amounts of things. If all your stuff looks as clean as it does it the pics you could easily do it. If the time ever comes that you want to do bags worth of material or outdoor projects, LI is a great skill to have. There easy ways to make it without much risk as well
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25835843 - 02/24/19 11:15 PM (5 years, 10 months ago) |
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Thanks noise ! Your encouragement is making me study up on it.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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SumFunGi
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25835852 - 02/24/19 11:20 PM (5 years, 10 months ago) |
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Quote:
Noisecontrol said: I will say that LI is a real benefit to saveing time while innoculating large amounts of things. If all your stuff looks as clean as it does it the pics you could easily do it. If the time ever comes that you want to do bags worth of material or outdoor projects, LI is a great skill to have. There easy ways to make it without much risk as well
How do you do your LI from a clean agar plate?
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bulbstoo
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25835856 - 02/24/19 11:21 PM (5 years, 10 months ago) |
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Quote:
Noisecontrol said: I will say that LI is a real benefit to saveing time while innoculating large amounts of things. If all your stuff looks as clean as it does it the pics you could easily do it. If the time ever comes that you want to do bags worth of material or outdoor projects, LI is a great skill to have. There easy ways to make it without much risk as well
What tek do you recommend for starting with LI? I'm a bit intimidated, but quickly learning about agar technique, having success, and looking to keep expanding.
Appreciate your help!
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25835858 - 02/24/19 11:22 PM (5 years, 10 months ago) |
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If you get you some SHIPs, or hell even just high temp silicone, you can make a hole in a jar lid and fill it with an injection site. Break your jar up once its fully colonized as normal but dont open it. From there take a sterilized syringe full of water (maybe very very slight amount of nutrient - optional) and inject into the jar the full content of the syringe, swirl the jar around, then tilt it on its side and suck it back up. This method is easiest with lil half pint jars - but ive even had success with full on quart jars carefully tipped to the side. I've made tons of LI like this, still have it in my fridge. If you can make clean spawn, you can make clean LI 
Edit: the remaining grain can still be used as spawn, or set to recolonize and you can fruit directly from it - just toss a bag over top and wait. If it was clean in the first place - clean it will remain - with common sterile technique of course
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Edited by Noisecontrol (02/24/19 11:25 PM)
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: bulbstoo]
#25835862 - 02/24/19 11:25 PM (5 years, 10 months ago) |
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I use a quart jar with 2 oz of water because I use 2 oz syringes. Simply make a jar lid with only an injection port and fill the jar with slightly more water than the syring you’re using can fit and starilize it. Then stranfer a section of clean agar and shake it till it dissolves and suck it through the self healing injection port. After that use it as a normal liquid culture syringe.
Edited by Thesunbeam (02/24/19 11:28 PM)
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SumFunGi
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25835880 - 02/24/19 11:46 PM (5 years, 10 months ago) |
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Quote:
Thesunbeam said: I use a quart jar with 2 oz of water because I use 2 oz syringes. Simply make a jar lid with only an injection port and fill the jar with slightly more water than the syring you’re using can fit and starilize it. Then stranfer a section of clean agar and shake it till it dissolves and suck it through the self healing injection port. After that use it as a normal liquid culture syringe.
Do you have issues sucking it back out? Seems like you would create a vacuum. But on the other hand, if you had a air filter on there as well, you couldn’t shake it... right? Isn’t there something with getting those filters wet?
I’ll have to try this. How many transfers should I do before using a plate (wedge) in this fashion?
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SumFunGi
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25835883 - 02/24/19 11:49 PM (5 years, 10 months ago) |
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Quote:
Noisecontrol said: If you get you some SHIPs, or hell even just high temp silicone, you can make a hole in a jar lid and fill it with an injection site. Break your jar up once its fully colonized as normal but dont open it. From there take a sterilized syringe full of water (maybe very very slight amount of nutrient - optional) and inject into the jar the full content of the syringe, swirl the jar around, then tilt it on its side and suck it back up. This method is easiest with lil half pint jars - but ive even had success with full on quart jars carefully tipped to the side. I've made tons of LI like this, still have it in my fridge. If you can make clean spawn, you can make clean LI 
Edit: the remaining grain can still be used as spawn, or set to recolonize and you can fruit directly from it - just toss a bag over top and wait. If it was clean in the first place - clean it will remain - with common sterile technique of course
I’ve read about this method, but it just has more steps. Plus I’d be worried about contams from the grain... only because I’ve yet to make grain from agar, and everyone talks about hidden things etc.. so I’m going to try the agar. To water first. Then maybe down the road once I’ve made as sure as possible my grain jar is clean, I’ll try this as well. Love all the options!
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: SumFunGi]
#25835884 - 02/24/19 11:52 PM (5 years, 10 months ago) |
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Yeah there is a vacuumed formed but It’s not very strong and I just use my hand to hold the plunger while pulling the needle out. You could use a whatman syringe filter glued to the lid as a waterproof air filter but I don’t find it necessary, especially with those tiny 10ml syringes. I would recommend making at least 2 transfers befor using agar for anything.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25835904 - 02/25/19 12:07 AM (5 years, 10 months ago) |
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Clean myc : clean grain : clean innoculant
I make my lids with a synthetic filter piece and a SHIP so no vacuum, they become multi-purpose this way and I can use them for whatever. The SFD are slightly hydrophobic at minor contact but sustained exposure to water will saturate them and this will wick contams in. Just be cautious not to get it wet, it's the same for all filters
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25835927 - 02/25/19 12:41 AM (5 years, 10 months ago) |
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I’ve had problems with wet filters. Placing jars in a cold moist room That’s why I’m trying unmodded lids aswell. I’m just scared about leaving the lids a little loose. I plan on loosening them after the first shake.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25836048 - 02/25/19 05:11 AM (5 years, 10 months ago) |
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Whats making your filters wet? Thats unusually unless you're pc'ing without foil or your grain is overly wet and you tip it upside down.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25836071 - 02/25/19 05:29 AM (5 years, 10 months ago) |
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They could have been way too wet. I used to drink When I did a lot of mycology Thankfully it’s now a thing of the passed Haven’t got any wet filters this time. I just remember them feeling wet when all my jars got messed up.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Fiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25837897 - 02/25/19 09:29 PM (5 years, 10 months ago) |
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Quote:
Thesunbeam said: I highly recommended agar LI. You can turn one wedge of agar into a few syringes that are easier to manage and more successful than transferring an agar wedge to each jar.
Oh wow. Shows what I know! I was under the impression that syringes were only for spores, I never even thought about or knew it was possible to turn agar into syringes!
 
P.S. let me not get too excited and build a flowhood first.
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Fiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25837901 - 02/25/19 09:31 PM (5 years, 10 months ago) |
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Quote:
Thesunbeam said: I highly recommended agar LI. You can turn one wedge of agar into a few syringes that are easier to manage and more successful than transferring an agar wedge to each jar. **LC
What does LI and LC stand for? *lol noobs
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery] 1
#25838078 - 02/25/19 11:42 PM (5 years, 10 months ago) |
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Liquid innoculant & liquid culture
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Fiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25838083 - 02/25/19 11:45 PM (5 years, 10 months ago) |
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Quote:
Noisecontrol said: Liquid innoculant & liquid culture

So, what's the difference between the two? Is LI spores and LC live mycelium?
If I had an agar plate, how would I go about getting it ready for a LC? You can't just put the agar plate into a blender and then suck it up with a syringe? That sounds like a contamination nightmare.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
#25838099 - 02/26/19 12:12 AM (5 years, 10 months ago) |
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Not all people with a moustache are a pedophile, but all pedophiles have moustaches.
i keed
LI can be live culture or spores, liquid culture is the already germinated organism.
You would establish clean myc on a plate and xfer that to a liquid nutrient suspension. Many use tools for agitating the culture once its growing in the suspension such as magnetic stir bars. This replicates shaking grain jars, only you do it much more often and typically over a long period of time.
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Fiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25838119 - 02/26/19 01:00 AM (5 years, 10 months ago) |
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Well that makes sense then. I am learning a lot.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
#25838130 - 02/26/19 01:10 AM (5 years, 10 months ago) |
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I like the method i mentioned because i hate waiting on LC to grow and it is a gamble if you're not certain of the cleanliness for whatever reason. Its a lot more convenient to me to extract some mycelial fibers from breaking spawn jars before i throw them to bulk. Literally all i have to do prep wise is keep a pc'd jar of sterile water and sterile syringes, and i can make LC of whatever variety i have a grain jar of. Technically, if you have a completely colonized plate, you could prep some nutrient suspension, get it sterile, and extract a syringe of it to then agitate the mycelium on the plate, squirt the syringe worth of nutrient suspension on it and suck it back up. This way you keep a jar of the nutrient and can make varieties of LC in individual syringes. I like this way if I have a bunch of varieties or clones i want to make LC from.
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Fiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25838132 - 02/26/19 01:13 AM (5 years, 10 months ago) |
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With oysters you can literally take rolls of toilet paper, soak them with boiling water, throw a little bit of spawn on them, tie them in a grocery bag, and just fruit them in a few weeks, or break them up into giant bins of "cold pasteurized" wheat straw.
wow. SO much easier! and so tasty.
But the hard work of cloning is worth it to grow more rare species!
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
#25838211 - 02/26/19 03:37 AM (5 years, 10 months ago) |
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Quote:
Fiery said:
Quote:
Noisecontrol said: Liquid innoculant & liquid culture

So, what's the difference between the two? Is LI spores and LC live mycelium?
If I had an agar plate, how would I go about getting it ready for a LC? You can't just put the agar plate into a blender and then suck it up with a syringe? That sounds like a contamination nightmare.
LI is just a wedge of agar diluted in water while lc is actualy growing the mycelium. I prefer my method because you don’t need to wait for it to grow/breed possible contamination and you don’t need any nutrients. If you leave LI sitting around it will turn into lc I guess, just wouldn’t wan t to leave it sitting because there is no gas exchange. Used fresh is best anyways. I use a jar with just an injection port and a little more water than what can fit in my syringe. Drop the agar in and give it a shake, then suck it though the injection port and use the syringe.
Edited by Thesunbeam (02/26/19 03:41 AM)
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25838652 - 02/26/19 09:14 AM (5 years, 10 months ago) |
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Time plays no part in the cleanliness. If it's gonna contaminate, that will be the outcome regardless. All of these processes require sterile technique. Your agar wedge in plain water has the same odds of contam as a nutrient suspension.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25848865 - 03/02/19 11:05 PM (5 years, 10 months ago) |
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UPDATE. Original batch of jars were just shaken up all were about 25 percent finished. So far so good. They all looked clean. Oyster myc is also a lot faster than cube. Just hoping my clone performs good. It was a crappy flush one pint brf crumbled to coir. but had a giant fruit in there. Exciting stuff.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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poisoned
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25849296 - 03/03/19 08:05 AM (5 years, 10 months ago) |
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according to my understanding, LC media offers way better conditions for contaminants to grow than grain or agar. Please correct me if I'm wrong.
Edited by poisoned (03/03/19 08:05 AM)
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: poisoned]
#25849348 - 03/03/19 08:43 AM (5 years, 10 months ago) |
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Theyre not comparable. Theres tons of different contam variables to each. Cleanliness is universal to all of them. Its a common misconception that your grain jars contam less than your culture mediums. In fact they probably contam more due to endospore rate, mostly being bacterial that you only see as an outcome of mycelial growth pattern, and ultimately short life of your fruiting substrate. If you're contamming at all, you should practice better sterile technique, not just attempt a different material.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25852757 - 03/04/19 06:31 PM (5 years, 10 months ago) |
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uPdate. It’s been over 24 hours since I shook them. All look great no contamination yet. Hella excited Anyways here is a photo of one of my jars I shook and also the oyster clone loving the oats.
 I know it’s lame updating small shit like this. But I like having a somewhere to record my progress.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Fiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25852869 - 03/04/19 07:22 PM (5 years, 10 months ago) |
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Quote:
Hoob33 said: uPdate. It’s been over 24 hours since I shook them. All look great no contamination yet. Hella excited Anyways here is a photo of one of my jars I shook and also the oyster clone loving the oats.
 I know it’s lame updating small shit like this. But I like having a somewhere to record my progress.
I've been watching your progress. It's not lame..
SO EXCITED!!
WOO!!!
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Fiery]
#25858675 - 03/07/19 03:07 PM (5 years, 10 months ago) |
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It’s been only 3 days. Here is the same jars. The other aa’s are a little behind.this one here I did a tiger drop. Super excited or these oysters tho. Just got some SOfT wood pellets for them. All I could find. Gonna do lions mane and shiitake once i get a hold of some cultures.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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EnDelfisE
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25858789 - 03/07/19 03:56 PM (5 years, 10 months ago) |
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Quote:
Hoob33 said: They could have been way too wet. I used to drink When I did a lot of mycology Thankfully it’s now a thing of the passed Haven’t got any wet filters this time. I just remember them feeling wet when all my jars got messed up.
wet filters have ruined some lcs of mine too before I realize simple tyvek glued with silicon it can get wet and actually I saw some steam coming up from the still warm jar( not hot ) and coming out of the pores of the thin tyvek as microscopic water droplets, I thought just because I tilted the jar filled with water upside-down and tyvek remained dry with no leaks that it was waterproof... little did I know ... after pcing I guess something happened and the pores expanded somehow , anyway these lcs half became bacterial
I tried to find sfds or watmans filters I even tried to build a weird frankenstain lid yeah thats how paranoid I got

but I think what helped me the most was getting a cheap " hard " tyvek sheet from a craft store rated at 105g/m² while soft was rated at 40 almost 3 times less , so I did some experimentation with that and it seems like its too thick for water to pass through not even when after pcing , I cut it in small round pieces and m3 micropore tape it in my gas exchange hole which is pretty big holes since I thought the more dense the tyvek the harder it would breath , seems to be serving me well after lots of jars and lcs , I haven't had any contams with it yet , now im checking to see how reusable is it, it doesnt seem to degrade after 4-5 pc cycles , I walso pc 3 hours at 11-12 pci I think cuz my European pc sux different standards here as it seems , damn I miss my presto when lived in the us! so cheap and so useful ! amazon even replayed the first one I got and destroyed on the first run... running out of water :S I told them it became inflated and round like an egg ready to explode while normal pressure cooking! next day the send me a new one! I love America <3
Edited by EnDelfisE (03/07/19 04:10 PM)
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EnDelfisE
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25858814 - 03/07/19 04:08 PM (5 years, 10 months ago) |
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Quote:
Hoob33 said: uPdate. It’s been over 24 hours since I shook them. All look great no contamination yet. Hella excited Anyways here is a photo of one of my jars I shook and also the oyster clone loving the oats.
 I know it’s lame updating small shit like this. But I like having a somewhere to record my progress.
damn that was fast!!! I remember my first time ever getting into growing mushrooms , before I discover this meca of knowledge mushroom <3 I got a spore syringe from a vendor called spores101 if I remember correctly ... I was so excited! I remember looking to verm in my small hometown ... garden stores didnt even know what it was back then haha finally found some! started watching rrs videos all day! everything seemed to be going great! and after a few days of birthing the cakes... oyster mushrooms apeard on them... I was freaking out! https://www.shroomery.org/forums/showflat.php/Number/16996445/page/2
the funny thing is cubies made their appearance too a few days after on the same cakes with the oysters! I cant complain tho, oysters made a delicious meal!
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: EnDelfisE]
#25866306 - 03/10/19 06:16 PM (5 years, 10 months ago) |
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UPDATE. here’s my AA+ and oyster clone jars. I think they are about ready to rock. What do you think? My other jars are still recovering. I have them in a tote and my house has been in the 50-60F. These two jars were stored in a higher/warmer spot. So they finished up faster.
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Joeyddr
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25866419 - 03/10/19 07:30 PM (5 years, 10 months ago) |
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Quote:
Hoob33 said: UPDATE. here’s my AA+ and oyster clone jars. I think they are about ready to rock. What do you think? My other jars are still recovering. I have them in a tote and my house has been in the 50-60F. These two jars were stored in a higher/warmer spot. So they finished up faster.

first pic looks blurry but the second looks pretty clean to me.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Joeyddr]
#25868904 - 03/12/19 01:33 AM (5 years, 10 months ago) |
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So today i spawned both these jars. I did the cubes with cvg used a 1-1.5 ratio. And I did the oyster with a mix of fir pellets,a keystone cardboard box I shreddd , And the rest of the cvg I had laying around. I tied a garbage bag around the basket. Gonna fruit them in my laundry room. Seems like more fresh air cirrculates in there. I smelled the oyster jar. I thought I fucked it up because it smelled so sweet And aromatic. Haha.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25870559 - 03/12/19 08:31 PM (5 years, 10 months ago) |
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What was your oyster ratio?
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25870753 - 03/12/19 10:23 PM (5 years, 10 months ago) |
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1 -5 ish.
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vikingsc
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25870861 - 03/12/19 11:40 PM (5 years, 10 months ago) |
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: vikingsc]
#25870994 - 03/13/19 01:59 AM (5 years, 10 months ago) |
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Weight or volume?
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25871039 - 03/13/19 02:30 AM (5 years, 10 months ago) |
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I did one quart of spawn to around 4.5,5 ish quarts of cardboard cvg and sawdust mix. I did it by volume in a measuring cup the best I could.i loosely measured it. Added what I thought it needed.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25871047 - 03/13/19 02:35 AM (5 years, 10 months ago) |
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Also. I shredded a beer box and added 3 cups of saw dust and pasteurized it in my pre heated insulated cooler all night and then mixed up my cvg from the previous night into it and mixed. I was gonna spawn bag it with sawdust but it’s ok I got a few more grains jars of these oysters to play with. Can’t wait.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33] 2
#25882365 - 03/18/19 05:22 PM (5 years, 9 months ago) |
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Update. Knocked up 10 more quarts 5 ps.cyans. For my outdoor projects. And 5 RW Got two shoeboxes into fruiting today. My oyster basket I’m letting go for another week. Because it’s bigger I also made 2 sawdust bags using lipa tek and my wild clone. Super excited. I also been cloning a few mushroom grow kits I now have pearl oyster and pink oyster in my collection. STOKED. I got so many things going on. It’s nice tho. Gets my mind off of real life bullshit. Myco zen

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vikingsc
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25882453 - 03/18/19 06:20 PM (5 years, 9 months ago) |
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Right on 
Do you have a separate thread for your outdoor projects?
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: vikingsc]
#25882532 - 03/18/19 07:12 PM (5 years, 9 months ago) |
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Not yet. It’s probably to late to spawn to woodchips but better late than never I will make one once I spawn the grains
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vikingsc
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25882643 - 03/18/19 08:23 PM (5 years, 9 months ago) |
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: vikingsc]
#25884955 - 03/20/19 01:56 AM (5 years, 9 months ago) |
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Was going to spawn a quart jar soon. And it got trich 95 percent colonized too Damn. It. Happens to everyone. Practice practice practice
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33] 1
#25896211 - 03/25/19 04:08 PM (5 years, 9 months ago) |
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Quick update. These AA+ shoeboxes have been fruiting for a few days now. Seems like they are takin some sweet time Had a jar theta cracked in the pc and was hella wet. I threw a culture in I named shitty clone. I spawned it half in a plastic food container and the other half in this glass boot it’s actually super cool looking. Healthy and ryzo Also. Here is the to SOFT WOOD fuel pellet bags with my wild oyster clone.its pretty neat watching the progress on these
The basket with coir cardboard and sawdust is still in the bag. I’m planning on opening it in 7 days. I did take a peek. It’s looking awsome can’t wait to show everyone. Anyways here is some pics Ps. My AAs are all tomentose. Almost thought I was growing cobweb haha
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33] 1
#25897100 - 03/25/19 11:59 PM (5 years, 9 months ago) |
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Where you source sawdust from?
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25897115 - 03/26/19 12:16 AM (5 years, 9 months ago) |
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I have a few local places that carry fuel pellets. I called everyplace even a local fireplace store. No one had HWFP. I went with bear mountain brand because it’s douglas fir mostly. I’ve read people get decent results using softwood. Atleast it’s something until I find some good pellets.
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25897798 - 03/26/19 10:46 AM (5 years, 9 months ago) |
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Straw pellets are sometimes used for horse bedding, might be worth a look
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25901163 - 03/27/19 11:46 PM (5 years, 9 months ago) |
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Anyone else have AA+ take a long time to pin? Been 15 days and not a single pin or knot that I see
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heaze2010
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25901184 - 03/28/19 12:16 AM (5 years, 9 months ago) |
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I've read alot of posts over the years mentioning that they can be slow pinners, don't quote me but I believe someone on here said it took them a little over 3 weeks to get there AA+ to fruit, but who knows what the conditions and other factors were.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: heaze2010]
#25905033 - 03/29/19 11:35 PM (5 years, 9 months ago) |
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Update. Here is my mini laundry basket with 1qt of spawn. “Cloned wild oyster” I have no idea what to do with it so I think I’m putting it under my awning outside. Forecast is calling for rain the next few days. Hopefully it doesn’t dry out to bad.
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Edited by Hoob33 (03/30/19 12:07 AM)
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25914727 - 04/04/19 02:24 AM (5 years, 9 months ago) |
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Update. It’s april now and a few pins have finally showed up The little red container shitty clone is doing awsome. My aa+ shoebox doesn’t look as cool as I thought. Blobby hill I hope my next few shoeboxes look better
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EnDelfisE
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25914747 - 04/04/19 02:40 AM (5 years, 9 months ago) |
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Happy to see you going strong <3
Im curious to see how they will turn out !
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: EnDelfisE]
#25914749 - 04/04/19 02:41 AM (5 years, 9 months ago) |
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I’m hoping i get a few cool clones from each one. Can’t wait.
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EnDelfisE
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25914761 - 04/04/19 02:53 AM (5 years, 9 months ago) |
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and you will get some awesome ones! I love this feeling of surprise hehe
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Thesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: EnDelfisE]
#25915018 - 04/04/19 07:02 AM (5 years, 9 months ago) |
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How’s the oysters going?
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25915225 - 04/04/19 09:46 AM (5 years, 9 months ago) |
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My basket hasn’t fruited yet. It’s sitting outside I mist it a few times a day Here is one of my sawdust bag. Looks weird but Im Hoping that’s how plu.pulmanarious. Looks.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25916213 - 04/04/19 07:38 PM (5 years, 9 months ago) |
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My pearl bags made from straw developed some of that, not so creamy but more yellow and transparent, on the bottoms of the bags as i put them outside to fruit recently. I do believe yours is bacteria and mine is enzymes, though I am new to gourmets as well. I think the milky color is bacteria, at least from my experience
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25916223 - 04/04/19 07:43 PM (5 years, 9 months ago) |
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i think it’s trying to fruit or something. It’s almost colonized and I’ve was gifted a bag or oyster once and it had white blobs like this But this is a wild Phoenix oysters. Super hyped to see it fruit. I have 5 bags like this.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25916501 - 04/04/19 10:50 PM (5 years, 9 months ago) |
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Thats not good. If it isnt fully colonized or just very recently fully colonized and you have blobbing/pin mass, its stress fruiting due to a compromised substrate. How did you sterilize and inoculate that bad boy? Wood typically requires a consolidation period for the myc to prepare prior to fruiting
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25916534 - 04/04/19 11:08 PM (5 years, 9 months ago) |
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Followed lipa tek. Cup 5 cups of softwood pellets 6 cups of boiling hot water. One gypsum one not. The one you see here I used a half a cup of gysum. It doesn’t look moldy at all I’m gonna still fruit it outside. We’ve been having a winter like spring lately.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25916536 - 04/04/19 11:09 PM (5 years, 9 months ago) |
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Inculcated clone A2g grain to bag. Mixed as well as I could next time I’m not filling them up so much.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25916561 - 04/04/19 11:24 PM (5 years, 9 months ago) |
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Yikes that sounds like a clusterfuck of uncertainty to me. I dont typically use wood but I sterilize and pasteurize subs enough to know that you may have only partially sterilized but surpassed pasteurized. I dont even know if there are any beneficial organisms to have been preserved in the first place, i suppose that would depend on your type of wood and where it came from. I would project that it fruits however I don't think it will last.. thats a real ugly yellow you got there
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25916566 - 04/04/19 11:26 PM (5 years, 9 months ago) |
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Yeah my other bags look better for sure. I was thinking it was bacteria aswell when I first looked at it.
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25918263 - 04/05/19 07:32 PM (5 years, 9 months ago) |
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Looks like my shitty clone performed ok. May clone off another tub of them If i get a better cluster My aa shoebox looks lame kinda. Wierd blob.if it grows cool I may just clone it too
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25918445 - 04/05/19 08:57 PM (5 years, 9 months ago) |
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I see you've got nice surface conditions, however there are some wet spots on your pins for that aa+. Being that they're that young, that tells me your misting is up and your evap isn't matching. You been misting?
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25918487 - 04/05/19 09:26 PM (5 years, 9 months ago) |
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I misted one side earlier there wasn’t any visible beads on one half so I missed it.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25918645 - 04/05/19 11:35 PM (5 years, 9 months ago) |
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Give a lil more fresh air. Those blotches come from water on the caps and not evaporating off. Could also be from their initiation. Either way is fixed with more air
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25919053 - 04/06/19 08:23 AM (5 years, 9 months ago) |
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Talking about the white spots? Those aren’t from water drops if that you’re talking about
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25919686 - 04/06/19 04:02 PM (5 years, 9 months ago) |
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Let these out the bag over night they had a little fuzzy feet They look better now.
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Noisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
#25919916 - 04/06/19 06:18 PM (5 years, 9 months ago) |
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Nah not the 'warts' persay, the wet blotches. They're dark spots on the caps, techincally bacterial if you examine their contents under the scope
Those are some nice specimen, what flavor?
Shitty clone, i see. Shitty clone not so shitty man lol. Gotta perfect the pinset before deeming something bad genetics
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Edited by Noisecontrol (04/06/19 06:21 PM)
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Hoob33
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25920081 - 04/06/19 07:46 PM (5 years, 9 months ago) |
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I named it shitty clone because it the plate was kinda iffy. I decided to see what it would do. It’s mislabeled but it’s either gt or creeper. Same thing.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25921936 - 04/07/19 08:11 PM (5 years, 9 months ago) |
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-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Noisecontrol
Test Tube Baby



Registered: 05/07/15
Posts: 729
Last seen: 7 months, 20 days
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25922205 - 04/07/19 11:13 PM (5 years, 9 months ago) |
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Wtf is that
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Happy to help, happier to learn
Feel free to slide in my DM
To the man in a wheelchair who stole my camouflage jacket...
...you can hide but you can't run...
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25922217 - 04/07/19 11:21 PM (5 years, 9 months ago) |
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WHATRE THOSE!!!!!!!!! Haha Wonder how big it will get.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25929765 - 04/11/19 11:11 PM (5 years, 9 months ago) |
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Update. Here is my aa+ And my shitty clone boot. Thanks everybody.
 I love agar now. Fuck ms syringe grows .
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25933095 - 04/13/19 07:56 PM (5 years, 9 months ago) |
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Almost forgot about these outside Hecka drying up good thing i checked.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
Edited by Hoob33 (04/13/19 07:56 PM)
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EnDelfisE
Rider On The Storm


Registered: 09/07/12
Posts: 499
Loc: Temple of Demetra
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25937580 - 04/16/19 06:56 AM (5 years, 8 months ago) |
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awww!!! now that got me thinking to try clone one of these oysters I got from the farmers market and try this!!!
looking good brother!
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: EnDelfisE]
#25938273 - 04/16/19 03:23 PM (5 years, 8 months ago) |
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I found the original mushrooms cloned on a pile of dead alder wood I also cloned one of them mushroom grow kits from Home Depot. Hopefully it fruits like the kit did. Shit blew up. Can’t wait to sho off this basket once it finishes up Looking great so far
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25938812 - 04/16/19 08:13 PM (5 years, 8 months ago) |
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-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Noisecontrol
Test Tube Baby



Registered: 05/07/15
Posts: 729
Last seen: 7 months, 20 days
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25938998 - 04/16/19 10:08 PM (5 years, 8 months ago) |
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--------------------

Happy to help, happier to learn
Feel free to slide in my DM
To the man in a wheelchair who stole my camouflage jacket...
...you can hide but you can't run...
Trade List
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25944397 - 04/19/19 07:25 PM (5 years, 8 months ago) |
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 Here it is. I don’t think they will get any bigger Cool little project.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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Noisecontrol
Test Tube Baby



Registered: 05/07/15
Posts: 729
Last seen: 7 months, 20 days
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
#25944449 - 04/19/19 08:02 PM (5 years, 8 months ago) |
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Great job great flush
--------------------

Happy to help, happier to learn
Feel free to slide in my DM
To the man in a wheelchair who stole my camouflage jacket...
...you can hide but you can't run...
Trade List
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Hoob33
Three toed sloth head yo



Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 10 days, 18 hours
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
#25944455 - 04/19/19 08:06 PM (5 years, 8 months ago) |
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THank you man.
-------------------- when god hands you lemons you,FIND A NEW GOD!
  
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