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OfflineHoob33
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Registered: 02/25/17
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2nd ever agar attempt.Grow log. Pics.
    #25835512 - 02/24/19 08:39 PM (5 years, 10 months ago)

Yo.  Last year I went ahead and tried doing some agar to grain. HUGE FAIL. every jar I made contaminated. I gave up on agar and went back to ms syringe. What a gamble /shit show.
New Years resolution is to get it right this time.
I said SCREW WBS. went out finally found some oats. Made two batches. One was made following bods easy oat tek. Another batch I made was made following the RR dvd method. “24 hour soak “
went ahead and made a shit load of pasty plates and went though them real quick. Made transfers,cloned the biggest fruit from an old ms grow. And germinated spores ects...my contamination rate has significantly gone done the more i practice sterile technique.

So here I am today with several clean cultures I’ve expanded into several other plates.  Just dropped some wedges in my 24 hour soak grain and it’s been a week since I dropped some wedges in the first batch. Also I made a few different lids. “Multi spore .tyvek .unmodded “ whatever works for me is what I’m sticking to.
Here is some photos

One of several agar plates



Different style lids. I’m hoping this tote will keep them safe.



First batch wedges dropped



One week later
Aa+ germinated from spore t-8
Golden teacher clone t 6


Wild  oyster clone taken from this cluster sorry for the blurry ness it’s  pulmonarius



Here is a t-7 wedge I did last week of it.

Here is the A2g I made today using 24 hour soaked grain.


I noticed doing 24 hour soak and a longer drying period the grains came out prefect. The first batch had to be shaken to even out some moisture but it looks amazing compared to messy ass bird seeds
Anyways. This is my second attempt I don’t wanna give up I love this hobby and any advise and input are more than welcome.
Also I have a few t-3 pan cyans on grain. Haha
I said I wasn’t gonna do to many projects on agar. Keep it simple  but it’s hard when theirs so many possibilities.


--------------------
when god hands you lemons you,FIND A NEW GOD! 

Edited by Hoob33 (02/24/19 08:42 PM)

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25835782 - 02/24/19 10:40 PM (5 years, 10 months ago)

:seriousthumbsup:


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OfflineThesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25835800 - 02/24/19 10:46 PM (5 years, 10 months ago)

I highly recommended agar LI. You can turn one wedge of agar into a few syringes that are easier to manage and more successful than transferring an agar wedge to each jar.

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OfflineHoob33
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Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25835806 - 02/24/19 10:50 PM (5 years, 10 months ago)

I was thinking making LC  But I don’t wanna introduce another source of contamination into the equation.if you know what I mean.  Maybe in a few weeks once I get this down a little more.
The speedy colonization of a LI is titalating haha.


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when god hands you lemons you,FIND A NEW GOD! 

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OfflineThesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25835832 - 02/24/19 11:05 PM (5 years, 10 months ago)

I like LI so much because unlike making multiple transfers to jars where you might pick up a contam in between jars, you only transfer to one jar and then the culture is contained within the LI jar and the syringe.

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25835838 - 02/24/19 11:10 PM (5 years, 10 months ago)

I will say that LI is a real benefit to saveing time while innoculating large amounts of things. If all your stuff looks as clean as it does it the pics you could easily do it. If the time ever comes that you want to do bags worth of material or outdoor projects, LI is a great skill to have. There easy ways to make it without much risk as well


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OfflineHoob33
Three toed sloth head yo
I'm a teapot User Gallery


Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25835843 - 02/24/19 11:15 PM (5 years, 10 months ago)

Thanks noise ! Your encouragement is making me study up on it.


--------------------
when god hands you lemons you,FIND A NEW GOD! 

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OfflineSumFunGi
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Registered: 12/27/17
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25835852 - 02/24/19 11:20 PM (5 years, 10 months ago)

Quote:

Noisecontrol said:
I will say that LI is a real benefit to saveing time while innoculating large amounts of things. If all your stuff looks as clean as it does it the pics you could easily do it. If the time ever comes that you want to do bags worth of material or outdoor projects, LI is a great skill to have. There easy ways to make it without much risk as well




How do you do your LI from a clean agar plate?

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Offlinebulbstoo
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25835856 - 02/24/19 11:21 PM (5 years, 10 months ago)

Quote:

Noisecontrol said:
I will say that LI is a real benefit to saveing time while innoculating large amounts of things. If all your stuff looks as clean as it does it the pics you could easily do it. If the time ever comes that you want to do bags worth of material or outdoor projects, LI is a great skill to have. There easy ways to make it without much risk as well




What tek do you recommend for starting with LI? I'm a bit intimidated, but quickly learning about agar technique, having success, and looking to keep expanding.

Appreciate your help!


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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25835858 - 02/24/19 11:22 PM (5 years, 10 months ago)

If you get you some SHIPs, or hell even just high temp silicone, you can make a hole in a jar lid and fill it with an injection site. Break your jar up once its fully colonized as normal but dont open it. From there take a sterilized syringe full of water (maybe very very slight amount of nutrient - optional) and inject into the jar the full content of the syringe, swirl the jar around, then tilt it on its side and suck it back up. This method is easiest with lil half pint jars - but ive even had success with full on quart jars carefully tipped to the side. I've made tons of LI like this, still have it in my fridge. If you can make clean spawn, you can make clean LI :smile: :cheers:


Edit: the remaining grain can still be used as spawn, or set to recolonize and you can fruit directly from it - just toss a bag over top and wait. If it was clean in the first place - clean it will remain - with common sterile technique of course


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Edited by Noisecontrol (02/24/19 11:25 PM)

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OfflineThesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: bulbstoo]
    #25835862 - 02/24/19 11:25 PM (5 years, 10 months ago)

I use a quart jar with 2 oz of water because I use 2 oz syringes. Simply make a jar lid with only an injection port and fill the jar with slightly more water than the syring you’re using can fit and starilize it. Then stranfer a section of clean agar and shake it till it dissolves and suck it through the self healing injection port. After that use it as a normal liquid culture syringe.

Edited by Thesunbeam (02/24/19 11:28 PM)

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OfflineSumFunGi
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Registered: 12/27/17
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25835880 - 02/24/19 11:46 PM (5 years, 10 months ago)

Quote:

Thesunbeam said:
I use a quart jar with 2 oz of water because I use 2 oz syringes. Simply make a jar lid with only an injection port and fill the jar with slightly more water than the syring you’re using can fit and starilize it. Then stranfer a section of clean agar and shake it till it dissolves and suck it through the self healing injection port. After that use it as a normal liquid culture syringe.




Do you have issues sucking it back out?  Seems like you would create a vacuum.  But on the other hand, if you had a air filter on there as well, you couldn’t shake it...  right?  Isn’t there something with getting those filters wet?

I’ll have to try this.  How many transfers should I do before using a plate (wedge) in this fashion?

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OfflineSumFunGi
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25835883 - 02/24/19 11:49 PM (5 years, 10 months ago)

Quote:

Noisecontrol said:
If you get you some SHIPs, or hell even just high temp silicone, you can make a hole in a jar lid and fill it with an injection site. Break your jar up once its fully colonized as normal but dont open it. From there take a sterilized syringe full of water (maybe very very slight amount of nutrient - optional) and inject into the jar the full content of the syringe, swirl the jar around, then tilt it on its side and suck it back up. This method is easiest with lil half pint jars - but ive even had success with full on quart jars carefully tipped to the side. I've made tons of LI like this, still have it in my fridge. If you can make clean spawn, you can make clean LI :smile: :cheers:


Edit: the remaining grain can still be used as spawn, or set to recolonize and you can fruit directly from it - just toss a bag over top and wait. If it was clean in the first place - clean it will remain - with common sterile technique of course




I’ve read about this method, but it just has more steps.  Plus I’d be worried about contams from the grain...  only because I’ve yet to make grain from agar, and everyone talks about hidden things etc..  so I’m going to try the agar. To water first.  Then maybe down the road once I’ve made as sure as possible my grain jar is clean, I’ll try this as well.  Love all the options!

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OfflineThesunbeam
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: SumFunGi]
    #25835884 - 02/24/19 11:52 PM (5 years, 10 months ago)

Yeah there is a vacuumed formed but It’s not very strong and I just use my hand to hold the plunger while pulling the needle out. You could use a whatman syringe filter glued to the lid  as a waterproof air filter but I don’t find it necessary, especially with those tiny 10ml syringes. I would recommend making at least 2 transfers befor using agar for anything.

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25835904 - 02/25/19 12:07 AM (5 years, 10 months ago)

Clean myc : clean grain : clean innoculant

I make my lids with a synthetic filter piece and a SHIP so no vacuum, they become multi-purpose this way and I can use them for whatever. The SFD are slightly hydrophobic at minor contact but sustained exposure to water will saturate them and this will wick contams in. Just be cautious not to get it wet, it's the same for all filters


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OfflineHoob33
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Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25835927 - 02/25/19 12:41 AM (5 years, 10 months ago)

I’ve had problems with wet filters. Placing jars in a cold moist room
That’s why I’m trying unmodded lids aswell. I’m just scared about leaving the lids a little loose.
I plan on loosening them after the first shake.


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when god hands you lemons you,FIND A NEW GOD! 

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OfflineNoisecontrol
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Hoob33]
    #25836048 - 02/25/19 05:11 AM (5 years, 10 months ago)

Whats making your filters wet? Thats unusually unless you're pc'ing without foil or your grain is overly wet and you tip it upside down.


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OfflineHoob33
Three toed sloth head yo
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Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Noisecontrol]
    #25836071 - 02/25/19 05:29 AM (5 years, 10 months ago)

They could have been way too wet.
I used to drink When I did a lot of mycology
Thankfully it’s now a thing of the passed
Haven’t got any wet filters this time.
I just remember them feeling wet when all my jars got messed up.


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when god hands you lemons you,FIND A NEW GOD! 

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InvisibleFiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25837897 - 02/25/19 09:29 PM (5 years, 10 months ago)

Quote:

Thesunbeam said:
I highly recommended agar LI. You can turn one wedge of agar into a few syringes that are easier to manage and more successful than transferring an agar wedge to each jar.




Oh wow. Shows what I know! I was under the impression that syringes were only for spores, I never even thought about or knew it was possible to turn agar into syringes!

:fuckyeah::themoreyouknow:

P.S. let me not get too excited and build a flowhood first.

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InvisibleFiery
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Re: 2nd ever agar attempt.Grow log. Pics. [Re: Thesunbeam]
    #25837901 - 02/25/19 09:31 PM (5 years, 10 months ago)

Quote:

Thesunbeam said:
I highly recommended agar LI. You can turn one wedge of agar into a few syringes that are easier to manage and more successful than transferring an agar wedge to each jar. **LC




What does LI and LC stand for? *lol noobs :loldongs:

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