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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Boogieman47
Let's boogie


Registered: 03/05/16
Posts: 9,712
Loc: Under your bed
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Just drop the whole puck tiger drop that bitch
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: Boogieman47] 3
#25828697 - 02/21/19 08:06 PM (5 years, 9 days ago) |
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Any portion of it... I hold the plate in my right hand,turned facing down at a 45Β°angle, scalpel in my left with the handle against my palm, and the blade run out between my fingers, pick up the 2 pieces of taped lid/ring action duo with my left hand,and spatula 8 of 16 "squares" (3 vertical and 3 horizontal cuts) into each of 2 jars per plate, using the scalpel turned on it's side...
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 6 days, 9 hours
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Re: Cultivation General Discussion [Re: Caps McGee]
#25828866 - 02/21/19 09:20 PM (5 years, 9 days ago) |
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Cultivation General Discussion [Re: Failboat]
#25828877 - 02/21/19 09:25 PM (5 years, 9 days ago) |
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Might be worth mentioning that I'm left handed... might be a pain in the dick for a righty without swapping orientation
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 31 minutes
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Re: Cultivation General Discussion [Re: Caps McGee]
#25829199 - 02/22/19 03:26 AM (5 years, 9 days ago) |
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Quote:
stareatclouds said: Dude, check it out. It's revolutionary, IMO, and makes LC as easy as possible. Essentially it's poking a plate of clean myc with a syringe and shooting that into an LC solution. Like the easiest way to transfer and such a small piece, it's easier to get a clean transfer.
Speaking of lc, what is the minimum time you would let broth sit before inoculation?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: tryptkaloids] 3
#25829205 - 02/22/19 03:38 AM (5 years, 9 days ago) |
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I'm unsure if you mean inoculating the LC broth or inoculating grains or whatever with the resulting LC. I'd wait until the liquid is room temperature before I inoculate an LC. How long I wait until I use it depends on a lot of shit, e.g., volume of liquid, whether I'm aspirating into syringes or pouring, what I'm trying to inoculate, etc.
I've only used LC a few times, though, and have strictly done LI. While the majority of my LC's have been successful, I'm very far from an expert on them. One thing I've done on small-scale and planned to experiment more with are single shot LC's in baby food jars. The minimum time there would be a thick colony throughout the media. They make for easier aspirating and you can dump them into bags very easily.

This was a sterilized bag of oats + CVG inoculated with an LC in a baby food jar in my SAB. I have a bunch of little uses for BFJ's, including slants, regular plates, etc. Plan on writing a Tek on them when I have more free time.
Also I think it was you asking for it, but I did record me doing the Josex pokes. Or maybe you wanted Pasty's EZ LC. I believe I recorded that as well. I'll get them up one of these days.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 31 minutes
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I made up some lc a week ago and never inoculated it. Is it still good?
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
stareatclouds said:

Nice
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 4 months, 5 days
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K.. i need to use bigger wedges.. i usually use about a quarter of that. Might explain how slow my stuff moves.
Quote:
stareatclouds said:

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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,647
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Quote:
stareatclouds said: Wow. That is not something I expected. Damn, that's crazy.
You weren't expecting it, the rest of us thought it was a bit late in showing up.

Humble too, makings of a great TC.
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π
π΄π°πΌ π΅πΎπΈπ»
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caghca
Me


Registered: 12/04/18
Posts: 45
Last seen: 4 years, 4 months
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Re: Cultivation General Discussion [Re: bodhisatta]
#25829554 - 02/22/19 08:47 AM (5 years, 8 days ago) |
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If y'all don't mind me slipping in a few questions real quick, that'd be great!. When is a flush considered done on pf tek? One of the cakes has one mush that is about 4 days old, and then a bunch of newer pins coming. Is that first mush considered a flush? Or do I wait to stop seeing pins before the flush is done? Second dilemma is, why the thin mycelium stretching out? to the left of this pic: 
Heres more of the other cake.

Edited by caghca (02/22/19 08:48 AM)
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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Re: Cultivation General Discussion [Re: bodhisatta]
#25829573 - 02/22/19 08:54 AM (5 years, 8 days ago) |
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Quote:
bodhisatta said:
Quote:
stareatclouds said:

Nice
That is an excellent GIF.
Does anybody see a difference in success rates using polyfil lids flipped vs gasket side down? I've been using them flipped since they're easier to take off for inoculation after PCing.
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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icetech



Registered: 08/21/17
Posts: 3,450
Loc: FSM's loving noodles.
Last seen: 4 months, 5 days
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Re: Cultivation General Discussion [Re: juniperus]
#25829592 - 02/22/19 09:00 AM (5 years, 8 days ago) |
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Quote:
juniperus said:
Quote:
bodhisatta said:
Quote:
stareatclouds said:

Nice
That is an excellent GIF.
Does anybody see a difference in success rates using polyfil lids flipped vs gasket side down? I've been using them flipped since they're easier to take off for inoculation after PCing.
I have always used them flipped and rarely ever have contam problems (only times i got contams were when i was rushing and knew i was fucking myself and still did it)
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juniperus
Frim Fram Sauce


Registered: 12/02/18
Posts: 718
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Re: Cultivation General Discussion [Re: icetech]
#25829604 - 02/22/19 09:05 AM (5 years, 8 days ago) |
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Quote:
icetech said: I have always used them flipped and rarely ever have contam problems (only times i got contams were when i was rushing and knew i was fucking myself and still did it)
Man, have I been there. My experience has been similar, just curious to hear if it was the same for others.
-------------------- To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: Mr Piggy]
#25829659 - 02/22/19 09:33 AM (5 years, 8 days ago) |
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Quote:
tryptkaloids said: I made up some lc a week ago and never inoculated it. Is it still good?
In theory, it should remain sterile, but how confident would you be that nothing got through? It's up to you. Liquids are capable of inoculating lots of grain and don't reveal contams as easy as other substrates so the importance of cleanliness is stressed. It's a teaspoon of malt w/ water and 45m in the PC to get a new, clean batch, so why not just do that?
Quote:
icetech said: K.. i need to use bigger wedges.. i usually use about a quarter of that. Might explain how slow my stuff moves.
I only shot that for folks requesting videos/visual aid on techniques. I have only went A2G a handful of times and have almost exclusively used LI > G2G since I started. There are many ways to skin a cat, but I couldn't tell you the optimal wedge size with any reliable data of my own, so keep that in mind. Fortunately it's easy to make plates and test stuff yourself and know for sure. 
How many wedges, size, placement, shaking initially or not, softness of agar, etc. All things that could be tested out by folks.
Quote:
Mr Piggy said:
Quote:
stareatclouds said: Wow. That is not something I expected. Damn, that's crazy.
You weren't expecting it, the rest of us thought it was a bit late in showing up.

Humble too, makings of a great TC.
Thanks, bud. I think you gave me shit when I first joined for a Shroomery member showing me a cyan patch, ha. Cool to see you still around.
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Thedillestpickle
cultured



Registered: 02/02/16
Posts: 1,170
Loc: Canada
Last seen: 3 years, 8 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25829797 - 02/22/19 11:08 AM (5 years, 8 days ago) |
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Quote:
tryptkaloids said: I made up some lc a week ago and never inoculated it. Is it still good?
I made some liquid culture and let it sit for 2 weeks. It got a contam after this time and what I learned was not to trust my wattman filters so much, and now I keep tin foil over the top of all LC jars. In theory, if everything is kept sterile, a LC should be good to use for a long time, probably at least a year. As with all foods, time, temperature, and oxygen will degrade the quality, so it will eventually become less than ideal but who knows how long that would take. It would take a long time.
I use LC exclusively.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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What size of Whatman do you have on there? No other modifications on the lid, such as SHIP or anything? Plastic or metal?
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,647
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Quote:
stareatclouds said: Thanks, bud. I think you gave me shit when I first joined for a Shroomery member showing me a cyan patch, ha. Cool to see you still around.
OMG, that was when I used to get shithoused and post on here. I remember that.
Sorry!

You'll be happy to know I don't drink anymore.
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π
π΄π°πΌ π΅πΎπΈπ»
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Caps McGee
Grandaddy Smurfshack



Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Re: Cultivation General Discussion [Re: Mr Piggy] 1
#25830111 - 02/22/19 01:44 PM (5 years, 8 days ago) |
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I use polyfil stuffed lids flipped for easier opening with no issues... seems i stuff a much smaller amount than most, ball about half the size of a ping pong ball, slightly pushed from one side, and then pulled into place really tightly...
Mr. Pig, how long you been sober? Pushing 3 years myself: quit smoking cigarettes Sunday morning around 3:30 too! Feels good, congrats to you for staying sober brother man!
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