Home | Community | Message Board


Everything Mushrooms
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon ½ Pint Jars, Agar

Jump to first unread post. Pages: 1
Offlinerahrah
Stranger
Female User Gallery


Registered: 01/23/19
Posts: 39
Last seen: 1 year, 7 months
Using agar to make a LC syringe in 1 step?
    #25825433 - 02/20/19 05:55 PM (2 years, 9 days ago)

I think I read this somewhere recently, but, for those of us with less-than-sterile workspaces, can you take a colonized, no-pour agar jar with a SHIP, inject sterile water inside & leave syringe in, shake it up, suck the liquid back into the syringe and use this LC to inoculate grains?

If so, does this method have a name? Would there be enough mycelium in the water just by shaking or does anyone have any tips for getting the most mycelium into the water without taking the lid off?

I've read about agar wedges to LC, but would like to cut out as many steps as I can right now to avoid contamination.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,639
Loc: Under your bed Flag
Trusted Cultivator
Re: Using agar to make a LC syringe in 1 step? [Re: rahrah]
    #25825445 - 02/20/19 06:00 PM (2 years, 9 days ago)

https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261 


That isnt the one I was thinking .. there is one somewhere


--------------------


Edited by Boogieman47 (02/20/19 06:02 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleBoogieman47
Let's boogie
Male User Gallery

Registered: 03/05/16
Posts: 9,639
Loc: Under your bed Flag
Trusted Cultivator
Re: Using agar to make a LC syringe in 1 step? [Re: Boogieman47]
    #25825450 - 02/20/19 06:04 PM (2 years, 9 days ago)



--------------------


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineLtLurker
Lost Sailor
 User Gallery


Registered: 01/03/18
Posts: 6,245
Loc: Borderlands Flag
Last seen: 3 hours, 3 minutes
Re: Using agar to make a LC syringe in 1 step? [Re: Boogieman47]
    #25825493 - 02/20/19 06:20 PM (2 years, 9 days ago)

It would be an LI(innoculant, plain water) not LC(Culture, nutritious water)

I'm more familiar with Pasty's take on it, but essentially looks the same as the one posted above.
https://www.shroomery.org/forums/showflat.php/Number/23740857

Shaking should be plenty to loosen some myc into the water.

As for sterility, you should be working in a SAB. Even if your environment is filthy, you can do sterile work inside it.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinerahrah
Stranger
Female User Gallery


Registered: 01/23/19
Posts: 39
Last seen: 1 year, 7 months
Re: Using agar to make a LC syringe in 1 step? [Re: LtLurker]
    #25825772 - 02/20/19 07:39 PM (2 years, 9 days ago)

Thank you both!! Plenty of info to get me going.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Amazon ½ Pint Jars, Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Agar>LC IGnosticAbhorI 834 4 03/27/05 09:57 AM
by IGnosticAbhorI
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 16,134 38 05/11/01 01:08 PM
by MAIA
* Agar ??? Semilanceata 444 4 02/28/04 07:20 AM
by Semilanceata
* Far into the LC Myc Matrix
( 1 2 3 all )
agar 8,327 44 05/26/05 03:36 PM
by Aeolus1369
* suitable sugars for LC (experiment with pic)
( 1 2 all )
lepiota 4,075 38 06/08/05 02:05 PM
by Hotnuts
* Lc
( 1 2 all )
IGnosticAbhorI 2,869 28 03/28/05 02:15 AM
by IGnosticAbhorI
* LC blooms carl 499 5 05/24/05 04:27 AM
by blackout
* Shaking LC jars after Inoculation? jtk 1,905 3 04/22/05 01:29 PM
by scatmanrav

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
83 topic views. 19 members, 103 guests and 51 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
World Seed Supply
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.043 seconds spending 0.013 seconds on 16 queries.