Home | Community | Message Board

Avalon Magic Plants
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < First | < Back | 736 | 737 | 738 | 739 | 740 | 741 | 742 | 743 | 744 | 745 | 746 | 747 | 748 | 749 | 750 | 751 | 752 | 753 | 754 | 755 | 756 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 11 hours, 1 minute
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Bph]
    #25819065 - 02/17/19 02:48 PM (5 years, 13 days ago)

The pressure lock doesnt have a gasket


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Offlinegizmodo
Woodland Creature
Male


Folding@home Statistics
Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 9 months
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: tryptkaloids]
    #25819067 - 02/17/19 02:49 PM (5 years, 13 days ago)

Tisket a tasket.
It puts the lotion in the basket.
Or else it gets the gasket.


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits

Extras: Filter Print Post Top
InvisibleJust_A_Noob
Breathing
Male User Gallery


Registered: 12/30/16
Posts: 6,809
Loc: PNW
Trusted Cultivator
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: gizmodo] * 2
    #25819099 - 02/17/19 03:10 PM (5 years, 13 days ago)

Since your askin
I put the oil on the gasket
So I’m not a basket
Case


--------------------

Wearing a mask is bad for my physical, emotional, and spiritual health.
Complying = Consent
Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links

Extras: Filter Print Post Top
Offlinegizmodo
Woodland Creature
Male


Folding@home Statistics
Registered: 06/21/18
Posts: 2,064
Loc: Behind You
Last seen: 2 years, 9 months
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Just_A_Noob] * 1
    #25819134 - 02/17/19 03:31 PM (5 years, 13 days ago)

:yess:


--------------------

One must be mindful to maintain their balance on the slippery earth.

Bod's Comprehensive Agar Resource
Gizmodo's Market Stall
Say No To Grow Kits

Extras: Filter Print Post Top
OfflineSpindlymass
wafer thin.
 User Gallery


Registered: 09/25/17
Posts: 957
Loc: Flag
Last seen: 5 days, 16 hours
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: tryptkaloids]
    #25819154 - 02/17/19 03:38 PM (5 years, 13 days ago)

Quote:

tryptkaloids said:
The pressure lock doesnt have a gasket





I think mine does. It's a thin white plastic if I remember.


--------------------
enough knowledge to get into trouble. Not nearly enough to get out again.

Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Caps McGee]
    #25819277 - 02/17/19 04:27 PM (5 years, 13 days ago)

Quote:

Caps McGee said:
I'm actually down to 3g agar powder and 4g LME to 525ml of water... may cut nutes further as I'm still getting partial tomentose growth from well established rhizomorphic cultures...



Your agar is already weak at 0.75% LME, but you expect ropier thicker more uniform growth from even less than 0.75%?

Whenever I've had cultures on 2% MEA they're usually growing rhizomorphic for the most part. I also notice that cultures on weaker agar don't have as much mass, no matter if it's rizo/tomentose, its almost always flimsier and weaker looking the weaker the agar gets.

What recipe did you have the cultures on before you moved them to 0.75%?


--------------------
Cakes inside Water Tub

Extras: Filter Print Post Top
InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Mateja]
    #25819645 - 02/17/19 07:22 PM (5 years, 13 days ago)

The less agar I use, the less nutes I can get away with... almost like they're more readily available to the culture? I started out standard MEA, 10g agar, 10g LME to 500ml of water... have been cutting dry ingredients in intervals since... stopped nutes at 6.5g, until my rhizomorphic cultures started displaying tomentose growth around 4.5g agar powder... dropped nutes  to 5g, regained rhizomorphic growth patterns... now at 3g agar powder, and getting tomentose at 4g LME to 525ml of water,  so going to pour the next round of plates with 3g agar powder, 3.5g LME to 525ml of water, see what I gather there...

Extras: Filter Print Post Top
OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,906
Loc: Washington Flag
Last seen: 8 months, 24 days
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Caps McGee]
    #25819774 - 02/17/19 08:06 PM (5 years, 13 days ago)

Quote:

Caps McGee said:
The less agar I use, the less nutes I can get away with... almost like they're more readily available to the culture? I started out standard MEA, 10g agar, 10g LME to 500ml of water... have been cutting dry ingredients in intervals since... stopped nutes at 6.5g, until my rhizomorphic cultures started displaying tomentose growth around 4.5g agar powder... dropped nutes  to 5g, regained rhizomorphic growth patterns... now at 3g agar powder, and getting tomentose at 4g LME to 525ml of water,  so going to pour the next round of plates with 3g agar powder, 3.5g LME to 525ml of water, see what I gather there...




That would make sense because the less agar but more nutrients the more easily accessible nutrients there would be vs being locked up by the agar so to speak


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat

Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Caps McGee]
    #25819992 - 02/17/19 10:31 PM (5 years, 13 days ago)

Quote:

Caps McGee said:
The less agar I use, the less nutes I can get away with... almost like they're more readily available to the culture? I started out standard MEA, 10g agar, 10g LME to 500ml of water... have been cutting dry ingredients in intervals since... stopped nutes at 6.5g, until my rhizomorphic cultures started displaying tomentose growth around 4.5g agar powder... dropped nutes  to 5g, regained rhizomorphic growth patterns... now at 3g agar powder, and getting tomentose at 4g LME to 525ml of water,  so going to pour the next round of plates with 3g agar powder, 3.5g LME to 525ml of water, see what I gather there...



So this post basically says you saw rizo on 2%nutes all the way down down to 1.2% Nutes and then it suddenly went back to tomentose again when agar thickness decreased slightly from 'unknown' to 0.85% thicnkess. Then rizo growth appeared once again as soon as you lowered the nutes from. 1.2% to 0.95%. (without altering agar thickness) But then the culture went tomentose once again as you lowered the recipe from 0.95%nutes to 0.75%nutes again.

And this observation is all preformed with the same clone culture going from plate to plate?

To clarify further: you had rizo on 2%nutes,then tomentose again at 1.2%nutes and then rizo on 0.95%nutes and again tomentose on 0.75%strength.

This is the same clone culture? Do you make transfers from neutral plates or do cultures either experience less food contra more food. If you take a donor clone culture from 1.2%MEA and drop it onto 0.75%MEA  and 2%MEA then the 1.2% donor culture will not behave and recover in the same way on 2.5% and 3%. Are you following my reasoning?


Edited by Mateja (02/18/19 12:04 AM)

Extras: Filter Print Post Top
OfflineMr. Funguy
Quasi Frodo
Male User Gallery

Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 4 years, 3 months
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Mateja]
    #25819997 - 02/17/19 10:34 PM (5 years, 13 days ago)

Quote:

Mateah said:
Quote:

Caps McGee said:
The less agar I use, the less nutes I can get away with... almost like they're more readily available to the culture? I started out standard MEA, 10g agar, 10g LME to 500ml of water... have been cutting dry ingredients in intervals since... stopped nutes at 6.5g, until my rhizomorphic cultures started displaying tomentose growth around 4.5g agar powder... dropped nutes  to 5g, regained rhizomorphic growth patterns... now at 3g agar powder, and getting tomentose at 4g LME to 525ml of water,  so going to pour the next round of plates with 3g agar powder, 3.5g LME to 525ml of water, see what I gather there...



So this post basically says you saw rizo until 1.2% Nutes and 0.85% Agar. Then rizo growth appeared again as you lowered the nutes from. 1.2% to 0.95%. But then it went tomentose again when you lowered the recipe to 0.75%nutes again.

And this observation is all preformed with the same clone culture going from plate to plate?






SCIENCE :alphabrain:


--------------------

Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: DnDRnD]
    #25820044 - 02/17/19 11:44 PM (5 years, 13 days ago)

Quote:

DnDRnD said:
Quote:

Caps McGee said:
stopped nutes at 6.5g, until my rhizomorphic cultures started displaying tomentose growth around 4.5g agar powder... dropped nutes  to 5g, regained rizo




That would make sense because the less agar but more nutrients the more easily accessible nutrients there would be



If that was true that less agar powder makes for easier access to nutrients aka more nutrient density then why did the culture from 1.2%nutes turn back to tomentose as agar powder stayed at 0.85% thickness. But then 0.95%nutes produces rizo again.


Another thing I can't comprehend is why wouldn't fully soluble MEA be proportionally and evebly distributed across the agar surface and also the entire agar mass? Just because the agar gel got a tiiiiiiny bit stiffer suddenly the malt extract could no longer be effectively digested by myc. That's sound no where near plausible in my ears. Myc pretty much just 'licks' the surface of the agar puch and doesn't bite down or dig into it so where is this theory grounded that softer agar makes the malt more acceccable to the myc to such degree that it readily goes from tomentose to rizo SIMPLY because nutrient density went down from 1.2% to 0.95%. And lest shall we forget that 1.2%nutes also produced rizo until agar thickness was reduced. By how much?

Like I posted in mbs journal I have 5 new agar types I poured yesterday so I have 8 different agar types and strengths combined. I have a few clones and just trying to figure out how we can compliment each other's tests to better come to right conclusions.

Imma start a new thread btw this is way off topic here but definitely deserves own thread for investigation. :cheers:


--------------------
Cakes inside Water Tub

Extras: Filter Print Post Top
OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,906
Loc: Washington Flag
Last seen: 8 months, 24 days
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Mateja]
    #25820081 - 02/18/19 12:36 AM (5 years, 13 days ago)

It turns rhizomorphic because its looking for nutrients so as the nutrients decrease the more rhizomorphic it becomes, that's my theory on why the, the more nutrients and less agar the more nutrients are on the surface while more agar and less nutrients then theres less nutrients on the surface

Ever notice how on nutrient strong recipes you tend to get slower more tomentose growth?


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat

Extras: Filter Print Post Top
OfflineHoob33
Three toed sloth head yo
I'm a teapot User Gallery


Registered: 02/25/17
Posts: 1,107
Loc: Rain forest
Last seen: 20 days, 10 hours
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: DnDRnD]
    #25820115 - 02/18/19 01:26 AM (5 years, 13 days ago)

I confess. I gave up on agar a year ago because all my jars came out contaminated.
I got new grains and just dropped some wedsges in them today. I been working a lot my sterile technique. I’m super hyped yet super scared it will happen again. It’s all I been thinking about.


--------------------
when god hands you lemons you,FIND A NEW GOD! 

Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Hoob33]
    #25820140 - 02/18/19 02:15 AM (5 years, 13 days ago)

:pm: me and I'll help you diagnose your issues you've been having.
First id like to know how you sterilize your grain, what filters you use on your grain jar lids and the procedure of inoculation.

Examining pics of contaminated jars can help pinpoint where the contam vectors are suffering. Could be your inoculation Tek, could be the cultures or jar lids for that matter, not to mention improper PC cycle. Start documentiong your grows in a new thread and update with pics along the way. That's the surest way to maximize your chance of success since you'll get help in real time and our 24h tech support team will give you accurate advice since you will have the steps documented in the thread. :goodluck:


--------------------
Cakes inside Water Tub

Extras: Filter Print Post Top
InvisibleYesum
Furry as Fuc
Male User Gallery


Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Mateja]
    #25820208 - 02/18/19 03:54 AM (5 years, 13 days ago)

Can I ask. Why do u guys care if the culture Expresses itself with rhizo growth as opposed to Tom growth. I've made my potato flake agar the same now for over a year.

20 flakes
10 light corn syrup
15 agar
1000 h20
* everything in grams.
Some cultures grow rhizo. Some don't. Sometimes I'll use 15g of flakes. But it really don't seem to make any difference. I'm just curious. I like the way rhizo growth looks too. But Tom growth is fine too.


--------------------

Extras: Filter Print Post Top
InvisibleMateja
 Unread Journal User Gallery

Registered: 07/14/16
Posts: 7,948
Loc: Here
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Yesum]
    #25820217 - 02/18/19 04:01 AM (5 years, 13 days ago)

I use healthy, viril, fast looking cultures to inoculate with.
Have zero preference about specifics when it comes to choosing working cultures.
The only preference I have is that the culture looks healthy and doesn't look off in any way.


And also I prefer to grow clones because I trip methodically.
A dry pount of the same clone and you can play in outer space for a long time with those amount of rocket fuel. Also reassuring to know exactly every time how far a refill of rocket fuel will take you and when it's time to head back to Houston. 🚀


--------------------
Cakes inside Water Tub

Edited by Mateja (02/18/19 04:08 AM)

Extras: Filter Print Post Top
InvisibleYesum
Furry as Fuc
Male User Gallery


Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Mateja]
    #25820224 - 02/18/19 04:05 AM (5 years, 13 days ago)

Yesh. I can dig that.

Kinda like here. I'm probably gonna streak more redboy spores because this clone seems to be slow in comparison to the others.


It looks healthy. But ots slow. For some reason. And it came from a beautiful specimen too. Which kinda sucks.


--------------------

Extras: Filter Print Post Top
InvisibleBumholio
What's the craic
 User Gallery


Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Yesum]
    #25820322 - 02/18/19 06:07 AM (5 years, 13 days ago)

Quote:

Yesum said:
Can I ask. Why do u guys care if the culture Expresses itself with rhizo growth as opposed to Tom growth.



My only concern is that its clean, I like rhizo cultures because they look fucking impressive, but as long as its clean I use it:shrug:


--------------------


"great things may come to those who wait, but only what's left by those who hustle"

Extras: Filter Print Post Top
OfflineDnDRnD
Hobby cultivator


Registered: 10/08/18
Posts: 2,906
Loc: Washington Flag
Last seen: 8 months, 24 days
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: Yesum]
    #25820324 - 02/18/19 06:08 AM (5 years, 13 days ago)

Quote:

Yesum said:
Can I ask. Why do u guys care if the culture Expresses itself with rhizo growth as opposed to Tom growth. I've made my potato flake agar the same now for over a year.

20 flakes
10 light corn syrup
15 agar
1000 h20
* everything in grams.
Some cultures grow rhizo. Some don't. Sometimes I'll use 15g of flakes. But it really don't seem to make any difference. I'm just curious. I like the way rhizo growth looks too. But Tom growth is fine too.




I don't really have a preference on rhizomorphic versus tomentose growth, but I think we are just trying to figure out whether it's purely genetics or how nutrition effects the growth patterns, although I do find rhizomorphic growth is easier to clean up on agar since it's easier to see through


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat

Extras: Filter Print Post Top
InvisibleStoneJackBaller
Male User Gallery


Registered: 01/09/19
Posts: 287
Loc: tumbled down shack
Re: TRUE CONFESSIONS - of an amateur mycologist - contest someday [Re: DnDRnD]
    #25820338 - 02/18/19 06:38 AM (5 years, 12 days ago)

First time posting long time lurking- soaking info like a sponge.  Couple of decent tubs so far.  My first time inoculating jars with a MS syringe, I loaded the still air box too full with jars then somehow spazzed due to lack of room and I managed to stick myself with the ms needle. :thumbdown::syringe:

Got blood on some jar lids and in still air box - not exactly sano... somehow still got healthy grain spawn.  The spores in my finger must not have incubated as no mushies growing out yet. :rolleyes:  not a finer moment and lesson learned.

This is a great website and hobby.  Looking forward to contributing.


--------------------

Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 736 | 737 | 738 | 739 | 740 | 741 | 742 | 743 | 744 | 745 | 746 | 747 | 748 | 749 | 750 | 751 | 752 | 753 | 754 | 755 | 756 | Next > | Last >

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Semi/automated small bulk substrate grow chamber SixTango 2,505 1 02/09/03 08:58 AM
by Dymentia
* Help! Yellow-Orange Semi-Hard Coating on Cakes Hannibal88 1,827 11 07/25/03 02:41 AM
by TxTec
* random innoculation gray1 894 3 05/03/01 04:10 PM
by gray1
* It's true what they say megaman3 1,748 19 04/22/03 08:37 AM
by cheesenoonions
* Where are the PROFESSIONAL MYCOLOGISTS? psilocybong 2,122 6 03/26/04 11:13 AM
by Anonymous
* Does this mean I am now a mycologist?
( 1 2 all )
Killjoy 4,102 22 07/04/17 07:00 PM
by LotKid
* casing semi-colonized cakes... sBUD 2,591 13 06/29/02 12:45 PM
by TheDnB_One
* Question for intermediate mycologists. Northernsoul 1,955 10 02/18/02 02:56 AM
by moxjet20

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
545,163 topic views. 21 members, 144 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.045 seconds spending 0.026 seconds on 14 queries.