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Translucent/weak mycellium from agar to grain (pic)
    #25817196 -

So trying my hand at agar, I mixed the agar up my self using telephone agar and light malt extract using 10g of each in 500ml water. Poured my plates and dropped some spores. I then did transfers of the outer edge to new plates and let it start to grow out.

I noticed that the mycellium seemed weak or even translucent. I dropped agar wedges into oat jars to see what happens and attached is the result. The mycellium appears to be moving through the jar but is maintaining that translucent or weak look.

So first question, are the jars likely FUBAR? Second question is there something I did in the agar prep that would make life hard on the mycellium, too little nutes, too much agar, etc?

Thanks for any suggestions!


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Re: Translucent/weak mycellium from agar to grain (pic) [Re: ninurta]
    #25817215 -

Well for one thing, if growth is weak on one medium, it's likely going to be slow on another. In your research you'll learn to be hunting agressive mycelium. Slow now doesn't mean it won't produce later tho.


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Re: Translucent/weak mycellium from agar to grain (pic) [Re: Smartattack]
    #25817226 -

What type of mushroom is this? Looks like pan mycelium..

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: nube424]
    #25817229 -

psilocybe cubensis

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: ninurta]
    #25817235 -

Def something wrong then.. how longs it been colonizing?

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: Smartattack]
    #25817236 -

Idk man, that doesn't look good, could be mold

If its not performing well for you on agar, then you picked a loser. Better to start over on agar until you have a nice clean well performing culture to work with.

This is what my jars look like


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I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: Johnny Dont]
    #25817244 -

Yea, even if u had weak growth on agar due to low nutrition, it would change when u drop it to grain. That's definitely not cubensis mycelium unless u just got unlucky with some ugly mycelium . Looks like mold tho tbh.

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: nube424]
    #25817309 -

And that maybe! But I dropped spores on 5 different plates and all 5 showed the same weak mycellium growth but in a pattern that looks like it came form the spore drops. I then pulled leading edge samples off three different plates and dropped on 10 different agar plates. All growth centered around the transferred wedge (so not like satellite colony). Seems odd I would propagate the same mold across all the different plates right?

Here are the two plates I didn't transfer with just to see what they did. If that helps at all. Did I just grow mold?


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Re: Translucent/weak mycellium from agar to grain (pic) [Re: ninurta]
    #25817319 -

Looks like molds man:feelsbadman:

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: mushboy]
    #25818111 -

Was this a print or syringe? Need some spores?

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: nube424]
    #25818289 -

the picture of the plate doesn't help me identify much but your jar is uber fubered. no es muy bueno.

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: ninurta]
    #26204611 -

ninurta said:
So trying my hand at agar, I mixed the agar up my self using telephone agar and light malt extract using 10g of each in 500ml water. Poured my plates and dropped some spores. I then did transfers of the outer edge to new plates and let it start to grow out.

I noticed that the mycellium seemed weak or even translucent. I dropped agar wedges into oat jars to see what happens and attached is the result. The mycellium appears to be moving through the jar but is maintaining that translucent or weak look.

So first question, are the jars likely FUBAR? Second question is there something I did in the agar prep that would make life hard on the mycellium, too little nutes, too much agar, etc?

Thanks for any suggestions!





What was the outcome here? Have you tried to spawn to bulk?

I keep on getting the exact same phenomenon as you describe with almost all my cultures on agar from different spore prints of different varieties. I have already changed my agar brand and my malt extract brand without any effect. Also now tried BRF agar plates, but its too early to tell, if it changes anything.
It seems to me that my cultures are tomentose most of the time and mostly remain tomentose when in oat jars. On agar they have a wide translucent leading edge and the center looks wispy. I always ask myself, if it's mold as well, but I spawned to bulk such grain jars once and they lead to fruits, although not strong growth really, but still it could not be mold after all.

Just curious, if you found out later, what the problem was...

Here are my jars, which look much like yours...



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Re: Translucent/weak mycellium from agar to grain (pic) [Re: Dr.Ivan]
    #26204617 -

Your jars look way better than the OP jar did. Especially that last picture of yours.

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: ShaperDreaming]
    #26204657 -

Hm, yeah, OP's jar could have a greyish tint to it, which is how I usually decide if it's mold or not.
But I have the same phenomenon with translucent mycelium on the plates.
Very strong in this pic. Don't know, what's causing this so far.

Edited by Dr.Ivan (09/23/19 12:49 PM)

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Re: Translucent/weak mycellium from agar to grain (pic) [Re: Dr.Ivan]
    #26288162 -

Found this helpful. The practice is to treat the translucent growth the same as you would white myc growth.

https://www.shroomery.org/forums/showflat.php/Number/25347656/fpart/1/vc/1

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