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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 4 days, 16 hours
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25803945 - 02/10/19 02:51 PM (5 years, 20 days ago)

I don't think it would work. LC is too thick to make it through the filter. I have trouble just getting it through
a 16 1/2 gauge needle sometimes.

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 36 minutes
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Re: Cultivation General Discussion [Re: Asura]
    #25803979 - 02/10/19 03:11 PM (5 years, 20 days ago)

I don't want the lc to go through, just the extra moisture


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleMycolorado
Hobbyist
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Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #25803996 - 02/10/19 03:25 PM (5 years, 20 days ago)

Anyone else having issues viewing the pic of the day thread?

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Cultivation General Discussion [Re: Mycolorado]
    #25804037 - 02/10/19 03:52 PM (5 years, 20 days ago)

No, still problems?

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OfflineStable Genius
Radicalised
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Registered: 09/26/18
Posts: 6,105
Loc: Wide Bay Orstralia
Last seen: 5 hours, 3 minutes
Re: Cultivation General Discussion [Re: Asura]
    #25804047 - 02/10/19 03:57 PM (5 years, 20 days ago)

No problems with pics here.


Clone culture vs spore culture.
Disasters are usually where I learn a lot.


These are the dishes I used for this round. Picked the best looking dish from each example.
The rhizomorphic dishes are from cloning.
The tomentose dishes are from a spore print.
Both on the same batch of Agar, both on the same batch of grain.

The rhizomorphic culture was kept out of the fridge to catch up and was larger than shown here when put on the grain. The tomentose plate came straight out of the fridge.

Yes the grain was too wet, and as well the room heated up past 90F, it's been very hot here.
Apart from fucking up the conditions, I found it obvious the tomentose jars colonised faster and handled the shitty conditions much better than the rhizomorphic jars.


Rhizomorphic jars                          Tomentose jars

The tomentose jars are now in shoeboxes, the rhizo jars are now in the garden.

Was the Rhizomorphic mycelium weaker to start with? Did it have anything or nothing to do with cloning?
I'd be interested to hear what others think.

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InvisiblemushboyMDiscord
modboy
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Registered: 04/24/05
Posts: 32,501
Loc: where?
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OG Cultivator
Re: Cultivation General Discussion [Re: Mycolorado] * 2
    #25804132 - 02/10/19 04:46 PM (5 years, 20 days ago)

Quote:

Mycolorado said:
Anyone else having issues viewing the pic of the day thread?




Yah there's never any pics just random off topic nonsense:crazy2:

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InvisibleMycolorado
Hobbyist
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Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: mushboy]
    #25804291 - 02/10/19 06:36 PM (5 years, 20 days ago)

Ha!  Working fine now, weird.

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OfflineMushroomBush
Energetic Anteater
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Registered: 01/20/19
Posts: 186
Loc: In The Sticks U.S.A Flag
Last seen: 1 year, 9 months
Re: Cultivation General Discussion [Re: Mycolorado]
    #25805808 - 02/11/19 01:21 PM (5 years, 19 days ago)



there is one spot on these jars that im concerned with i inocculated with the tiger drop on the 6th

i dont think the wbs was to wet if anything i thought it was to dry....PC for 90 min and let it vent steam for 15 min

is this wetspot or bacteria ? or do they look ok ?


--------------------
                                                               
                                                                                    Agar Recipe's

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: MushroomBush]
    #25805827 - 02/11/19 01:29 PM (5 years, 19 days ago)

They're definitely bacterial... you might get away with spawning them to slightly under field capacity coir only and hydrating if needed after full colonization... I'd start something fresh just in case these don't make it

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OfflineMushroomBush
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Re: Cultivation General Discussion [Re: Caps McGee]
    #25805859 - 02/11/19 01:45 PM (5 years, 19 days ago)

fuck i was gonna use these for g2g....not now

the only thing i can think of is to up my pc time, i was positive my sterile technique was good bc none of my agar plates have gotten any contams.

from my understanding baccillus can survive the pc if you dont cook it long enough, is this correct ?


--------------------
                                                               
                                                                                    Agar Recipe's

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OfflineMr. Funguy
Quasi Frodo
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Registered: 01/19/19
Posts: 1,077
Loc: Terra
Last seen: 4 years, 3 months
Re: Cultivation General Discussion [Re: MushroomBush]
    #25805874 - 02/11/19 01:53 PM (5 years, 19 days ago)




Hey guys, I'm not too worried as I'm in the learning process, is this cobweb? Not going to use it, as my agar is too watery and I still need to fine tune, just wondering so I can document it.


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InvisibleAyePlusS
Stony Danza
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Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: Mr. Funguy]
    #25805927 - 02/11/19 02:20 PM (5 years, 19 days ago)

Yeppers thats some kind of pin mold. You can tell by the black fruiting structures on the tips of the myc.


--------------------
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C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
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OfflineMr. Funguy
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Registered: 01/19/19
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Re: Cultivation General Discussion [Re: AyePlus]
    #25805947 - 02/11/19 02:31 PM (5 years, 19 days ago)

Awesome thank you.


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InvisibleR.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time Flag
Re: Cultivation General Discussion [Re: MushroomBush]
    #25806010 - 02/11/19 03:08 PM (5 years, 19 days ago)

Quote:

MushroomBush said:
fuck i was gonna use these for g2g....not now

the only thing i can think of is to up my pc time, i was positive my sterile technique was good bc none of my agar plates have gotten any contams.

from my understanding baccillus can survive the pc if you dont cook it long enough, is this correct ?




IMO, you may want to try 3 hour runs.
I always go 3 hours now @15psi on my runs and seen night at day improvement with this issue with oats. 
Never had a problem with straight white millet at 90.
If you can safely get up to 21 psi; 90 should be sufficient from what I have gathered. -edit- Also vent for 10 minutes so you don't have any air pockets.


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners

Edited by R.I.P.Zappa (02/11/19 03:13 PM)

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OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 1 month
Re: Cultivation General Discussion [Re: R.I.P.Zappa]
    #25806016 - 02/11/19 03:12 PM (5 years, 19 days ago)

Quote:

R.I.P.Zappa said:
Quote:

MushroomBush said:
fuck i was gonna use these for g2g....not now

the only thing i can think of is to up my pc time, i was positive my sterile technique was good bc none of my agar plates have gotten any contams.

from my understanding baccillus can survive the pc if you dont cook it long enough, is this correct ?




IMO, you may want to try 3 hour runs.
I always go 3 hours now @15psi on my runs and seen night at day improvement with this issue with oats. 
Never had a problem with straight white millet at 90.
If you can safely get up to 21 psi; 90 should be sufficient from what I have gathered.



Damn... I think I'm going to up my PC time.

Should I worry about bursting too many grains for PCing longer (oats)?

My shit has been more contaminated than I'd like lately.

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InvisibleR.I.P.Zappa
Myco Melyco


Registered: 03/30/18
Posts: 1,212
Loc: In Between Space and Time Flag
Re: Cultivation General Discussion [Re: ShaperDreaming]
    #25806027 - 02/11/19 03:15 PM (5 years, 19 days ago)

I run my two one pint white millet masters for g2g and never had em burst anymore than normal, I had the same concern when I upped my time but they came out just fine.

I also added a link about venting your pc above.


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it “reality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: MushroomBush]
    #25806065 - 02/11/19 03:32 PM (5 years, 19 days ago)

Quote:

MushroomBush said:
i was gonna use these for g2g....




:justno:

How long did you sterilize? It's suggested to go 2 hours, after letting it vent steam for 10 minutes and building pressure once the rocker is in place... id suspect your grain was too wet and has exacerbated the issue quite significantly... you might get lucky fruiting it, but subsequent G2G jars would likely stall out IMO

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OfflineShaperDreaming
Weirdo
Female User Gallery


Registered: 10/30/18
Posts: 3,429
Loc: United States Flag
Last seen: 2 years, 1 month
Re: Cultivation General Discussion [Re: Caps McGee]
    #25806090 - 02/11/19 03:41 PM (5 years, 19 days ago)

Not sure who you're asking Caps, but my process looks like this:

  • Bring up pressure to vent
  • Vent for 10 min
  • Bring up to 15psi
  • Set timer 2 hours
  • Regularly ensure that pressure doesn't drop too low (sometimes happens when balancing the temp)
  • Depressurize (often sleep too)


I usually do about 10pt jars or 5qt jars. Seeming good?

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: Cultivation General Discussion [Re: ShaperDreaming]
    #25806099 - 02/11/19 03:45 PM (5 years, 19 days ago)

Lol, the guy I quoted

Process looks sound to me... longer cook time (within reason) couldn't hurt though  :shrug:... 2 hours, @15psi, after a 10 minute steam vent is all I do for 10qt batches of oats

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OfflinePsilosopherr
A psilly goose
Other User Gallery


Registered: 02/15/12
Posts: 12,278
Last seen: 2 months, 10 days
Re: Cultivation General Discussion [Re: Inocuole]
    #25806119 - 02/11/19 03:59 PM (5 years, 19 days ago)

i'm snowed in and bored, somebody tell me something interesting they learned recently about mycology :begger:

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