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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Bad spore syringes?
    #25800570 -

Hey everyone, so i am getting back into the hobby and ordered some albino penis envy spores from didn't read the rules dot com. 1st syringe i ordered seems contaminated, Ive made a dozen or so agar plates with it and everyone contaminates. So i called the vendor they reship and Ive gotten nothing growing from this set of spores with about 10 agar dishes.Ive had luck with some golden teachers and b+ agar plates but cant get these ape spores to grow. Any tips or advice on getting these going. i really want to give these a shot but so far my luck has been way off.


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:cookiemonster:

Edited by bodhisatta (02/09/19 09:12 AM)

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Re: Bad spore syringes? [Re: CookieMonster]
    #25800605 -

First, take the vendors name out of your post.

How long ago did you inoculate the dishes with spores, it can't take 3 week to germinate sometimes. Also I find softer agar Recipies are better for germination.


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I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

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Re: Bad spore syringes? [Re: Johnny Dont]
    #25800633 -

Spores can be picky. Sometimes it can be quite hard to get germination and it takes a number of tries.


If you are working with agar, you should be purchasing spore prints instead of syringes. You get way more (enough to make many syringes), but also less chance of contamination than spores in solution.
Spore syringes are really just meant for PF Tek IMO.

Also if you aren't already, after you place a drop of spore solution on the plate to swipe the drop with a sterilized inoculation loop in a zig zag across the plate. This way you can better estimate where clean growth should be and hopefully catch clean myc before it grows over or into a mold colony or bacteria .


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Amateur Mycologists United
AMU Q&A

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Re: Bad spore syringes? [Re: mushpunx]
    #25800721 -

:whathesaid:

I was plating spores from a syringe from a reputable sponsor recently and got bacteria 90% of the time. Spores are inherently dirty since grows usually take place in open air. With a syringe, the spores are in a hydrated solution, which means bacteria can flourish.

The important thing is that even if there is some contamination, you're on agar so you can (hopefully) find some clean growth on the plate and transfer away to a new one, thus obtaining a clean culture. It may take a few transfers if the starting culture is very dirty.


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To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas

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Re: Bad spore syringes? [Re: Johnny Dont]
    #25800774 -

Why should he take the vendors name out of the post? There's no problem in telling us where you got the spores from, bud.

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Re: Bad spore syringes? [Re: Dr.Celium] * 1
    #25800798 -

Read the rules...


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To avoid all evil, to cultivate good, and to cleanse one's mind - this is the teaching of the Buddhas

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Re: Bad spore syringes? [Re: Dr.Celium]
    #25800799 -

Uh yeah, did you read the rules? Anyways, if I was committing a felony, would you want me to drag you into it?


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I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

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Re: Bad spore syringes? [Re: Johnny Dont]
    #25800831 -

Yup, I'm done with syringes. I've wasted a ton of time and money on syringes in the last couple years. Last APE I bought from everybody's favorite vendor (mine too) was complete trash. I tried agar, straight to oats, and WBS. Green mold every time.

But yet I was told by TC's here that vendor syringes are clean. Whatever

I have about 30 syringes on hand that I paid good money for, only 4 of them have given me ok results. Dirty dirty dirty

Maybe I'm just extremely unlucky. Lol

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Re: Bad spore syringes? [Re: Subfinder]
    #25800903 -

Subfinder said:
Yup, I'm done with syringes. I've wasted a ton of time and money on syringes in the last couple years. Last APE I bought from everybody's favorite vendor (mine too) was complete trash. I tried agar, straight to oats, and WBS. Green mold every time.

But yet I was told by TC's here that vendor syringes are clean. Whatever

I have about 30 syringes on hand that I paid good money for, only 4 of them have given me ok results. Dirty dirty dirty

Maybe I'm just extremely unlucky. Lol



It could be that the syringes are alright but your technique is flawed.
If you are using agar, you should be able to isolate good myc to get a clean culture going. Spore syringes aren't usually 100% clean, and I'm sure there is the odd super contaminated one, but you shouldn't be getting so many different syringes that cant be cleaned up on agar

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Re: Bad spore syringes? [Re: mushpunx]
    #25800957 -

mushpunx said:
Subfinder said:
Yup, I'm done with syringes. I've wasted a ton of time and money on syringes in the last couple years. Last APE I bought from everybody's favorite vendor (mine too) was complete trash. I tried agar, straight to oats, and WBS. Green mold every time.

But yet I was told by TC's here that vendor syringes are clean. Whatever

I have about 30 syringes on hand that I paid good money for, only 4 of them have given me ok results. Dirty dirty dirty

Maybe I'm just extremely unlucky. Lol



It could be that the syringes are alright but your technique is flawed.
If you are using agar, you should be able to isolate good myc to get a clean culture going. Spore syringes aren't usually 100% clean, and I'm sure there is the odd super contaminated one, but you shouldn't be getting so many different syringes that cant be cleaned up on agar



Doesn't seem to be enough viable spores compared to contaminates. I have no problem with prints whatsoever.

Plus, I have a few that always germinate no problem. I'm not a dumb person, I approach everything very scientifically and never jump to conclusions without extensive testing.

Pisses me off that I can't just buy prints of the types that I want. Been trying to grow APE forever. Can't buy a print and the syringes suck a dick.

Edit: I'd gladly send you the syringe so you can see for yourself.

Edited by Subfinder (02/09/19 08:23 AM)

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Re: Bad spore syringes? [Re: Subfinder]
    #25800960 -

How did you do your agar? Describe your process please.

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Re: Bad spore syringes? [Re: teladi]
    #25800970 -

Lately I've been using my oat water. 10 grams agar to 500ml oat water. No pour. Works for all my prints and 4 of 28 syringes. (Each a different kind)

I have tamps, pans, atl7, ps. Cyan, a few cubes and edibles going on agar. Literally 100's of plates. I'm no beginner, been growing on and off for 20+ years.

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Re: Bad spore syringes? [Re: Subfinder]
    #25800973 -

And then? Do you drop, squirt, streak, spray? What happens?

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Re: Bad spore syringes? [Re: teladi]
    #25800980 -

Ok, I streak a drop with my inoculation loop usually...sometimes I put a drop on the agar and streak it with the IL, sometimes I drip direct to the loop.

Then either nothing happens, or everything but myc grows. If I could get even a small amount of myc to germinate, I'd be ok.

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Re: Bad spore syringes? [Re: Subfinder]
    #25800989 -

Subfinder said:
Ok, I streak a drop with my inoculation loop usually...sometimes I put a drop on the agar and streak it with the IL, sometimes I drip direct to the loop.

Then either nothing happens, or everything but myc grows. If I could get even a small amount of myc to germinate, I'd be ok.



And this is with all 30 syringes??
Have you tried another vendor?


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Amateur Mycologists United
AMU Q&A

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Re: Bad spore syringes? [Re: mushpunx]
    #25801029 -

mushpunx said:
Subfinder said:
Ok, I streak a drop with my inoculation loop usually...sometimes I put a drop on the agar and streak it with the IL, sometimes I drip direct to the loop.

Then either nothing happens, or everything but myc grows. If I could get even a small amount of myc to germinate, I'd be ok.



And this is with all 30 syringes??
Have you tried another vendor?


Yes all 28...4 are fine, 24 are not. No, all from one (very trusted) vendor. Had something similar happen years ago with another trusted vendor.

I don't blame the vendor really. I have just come to the conclusion that syringes are pretty hit and miss. I don't even bother trying a replacement or whatever. I just donate to the cause or whatever.

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Re: Bad spore syringes? [Re: Subfinder]
    #25801032 -

Subfinder said:
But yet I was told by TC's here that vendor syringes are clean.


I highly doubt this.  Every TC and most able growers here know that spores are inherently dirty.  I’ve never heard anyone say syringes are clean, vendor or otherwise.  I’d recommend using less liquid, make way more plates and check your technique.  I’ve had terrible syringes before but 22/28 speaks to technique more than the syringes.


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🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: Bad spore syringes? [Re: Mycolorado]
    #25801039 -

Mycolorado said:
Subfinder said:
But yet I was told by TC's here that vendor syringes are clean.


I highly doubt this.  Every TC and most able growers here know that spores are inherently dirty.  I’ve never heard anyone say syringes are clean, vendor or otherwise.  I’d recommend using less liquid, make way more plates and check your technique.  I’ve had terrible syringes before but 22/28 speaks to technique more than the syringes.


Lol...I'll go find the qoute. Give me a minute.

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Re: Bad spore syringes? [Re: Subfinder]
    #25801045 -

Lol looking through your posts and bod says it should be like at least 80% success rate. That is pretty controversial, but what is true about it is that you should be having more success than this. You must address sterile work.


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Re: Bad spore syringes? [Re: Subfinder]
    #25801048 -

lol what?  It’s easy for some people to blame other factors for their failures when they’re not willing to acknowledge that their lack of skill is actually to blame.  :shrug:


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🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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