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Shroombie2
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Registered: 12/28/18
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Forgot to shake spore syringe 1
#25775953 - 01/29/19 04:26 AM (5 years, 25 days ago) |
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2 weeks ago, I sterilized and inoculated 4 jars of rye. I used a new spore syringe and at first, I forgot to shake it. After using some milliliters I realized that and shook the syringe and used it again. Since I forgot to shake the syringe, I used a lot. I used 20 milliliters for 2 jars each 500 ml. But still it didn't help, one of my jars now have not started to colonize while the other 3 started colonizing one week ago. I'm thinking I probably got all the spores out with the first milliliters before shake.
Can I inoculate that jar that has not started to colonize now or is it better to start over? I'm worried that there will not be enough spawn for a monotub.
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Buckthorn
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Re: Forgot to shake spore syringe [Re: Shroombie2]
#25775972 - 01/29/19 04:52 AM (5 years, 25 days ago) |
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I forget to shake sometimes.
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Errl_Shmirl
New Kid On The Block



Registered: 03/25/17
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Loc: Southern Plains
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Re: Forgot to shake spore syringe [Re: Buckthorn]
#25776001 - 01/29/19 05:23 AM (5 years, 25 days ago) |
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Being that most syringes have a visible clump in them. Spores are microscopic and that clump contains an unbelievably huge amount of them, which means that throughout the rest of the syringe there are also tons of them that cant be seen. It only takes a couple three of em to start up the process we all love so much.
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mushpunx
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Re: Forgot to shake spore syringe [Re: Errl_Shmirl]
#25776024 - 01/29/19 05:41 AM (5 years, 25 days ago) |
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First off Agar work is a *must* if you are working with grains. Grains are not forgiving the way BRF is. But if you insist on using spores to inoculate grain, 20ML is WAY too much dude. It will throw off the moisture content. You should only use a single drop or two to inoculate with a syringe.
Like he said above there are countless spores in the syringe. You only need two. Not shaking didn't matter at all really
Hope this helps!
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Shroombie2
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Re: Forgot to shake spore syringe [Re: mushpunx]
#25776030 - 01/29/19 05:47 AM (5 years, 25 days ago) |
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Thanks. That's very comforting. But I have forgot to shake the syringes before and then my BRF cakes never colonized. I will wait and see then, hopefully it will start soon. I didn't know agar was easier to work with than spore syringes, I need to learn that soon. I also need to learn the right dosage for inoculating. But I've been observing at least that, when I increase the dosage, the colonization takes place a lot faster.
Edited by Shroombie2 (01/29/19 05:48 AM)
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Vibe_Enthusiast
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Re: Forgot to shake spore syringe [Re: Shroombie2]
#25776061 - 01/29/19 06:22 AM (5 years, 25 days ago) |
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Never forget to shake it;]
You should be fine dude. Millions of spores are in there
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Vibe_Enthusiast
Mushroom Technician



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Re: Forgot to shake spore syringe [Re: Shroombie2]
#25776063 - 01/29/19 06:23 AM (5 years, 25 days ago) |
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Quote:
Shroombie2 said: Thanks. That's very comforting. But I have forgot to shake the syringes before and then my BRF cakes never colonized. I will wait and see then, hopefully it will start soon. I didn't know agar was easier to work with than spore syringes, I need to learn that soon. I also need to learn the right dosage for inoculating. But I've been observing at least that, when I increase the dosage, the colonization takes place a lot faster.
Agar is so worth it. I wish I would have started that from the get go. All you need is a DROP... more than that can actually be worse. You may have seen faster colonization because the myc was more aggressive than the others!
Also, some spores just don't germinate. It happens
Edited by Vibe_Enthusiast (01/29/19 06:24 AM)
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mushpunx
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Registered: 04/20/14
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Agar isn't necessarily easier, but it ensures a clean culture, while spore syringes are never 100% clean even with proper indoor spore printing. They frequently harbor bacteria or mold. Germinating the spores on agar gives us a 2d surface to view the culture and any contaminants that grew with it- we can then transfer a piece of clean mycelium tissue to another agar plate and end up with a clean culture. BRF/PF tek jars typically manage OK with spore syringes but grains are much more prone to contamination.
One more thing, until you start working with agar, I find it best to inoculate a few jars with just one drop or so each. Examine the colonized jars carefully and only select the cleanest to be used for grain to grain transfer in a still air box. Best chance of getting clean healthy spawn
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Shroombie2
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Re: Forgot to shake spore syringe [Re: mushpunx]
#25778324 - 01/30/19 04:18 AM (5 years, 24 days ago) |
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Thank, I've never actually had contamination problems with the spore syringes and my manufacturer guarantees me that they are contamination free.
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Shroombie2
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Last seen: 8 months, 26 days
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Re: Forgot to shake spore syringe [Re: Shroombie2]
#25778325 - 01/30/19 04:19 AM (5 years, 24 days ago) |
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Should I reinoculate the jar that is not colonizing to save the rye and time for sterilization or should I start over with new jar and rye?
Edited by Shroombie2 (01/30/19 04:22 AM)
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mushpunx
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Re: Forgot to shake spore syringe [Re: Shroombie2]
#25778458 - 01/30/19 06:24 AM (5 years, 24 days ago) |
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Just because you haven't yet, doesn't mean you won't , because you will! There is almost always bacteria in a spore syringe, could very well be lowering your yields
Never re inoculate,make a new one. Only use a single drop or two of spore solution
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bodhisatta 
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Re: Forgot to shake spore syringe [Re: Shroombie2]
#25778477 - 01/30/19 06:35 AM (5 years, 24 days ago) |
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Quote:
Shroombie2 said: Thank, I've never actually had contamination problems with the spore syringes and my manufacturer guarantees me that they are contamination free.
that's complete BS. spore prints are not axenic thus you can't make a contamination free spore syringe. there's always some level even if it's minuscule.
most syringes are made well enough they will work on PF cakes and easily get you clean growth, same for agar.
Quote:
Shroombie2 said: Should I reinoculate the jar that is not colonizing to save the rye and time for sterilization or should I start over with new jar and rye?
you should re-make them if you inoculated with 20mL to start.
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