Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineShroombie2
Stranger
Registered: 12/28/18
Posts: 189
Last seen: 8 months, 26 days
Forgot to shake spore syringe * 1
    #25775953 - 01/29/19 04:26 AM (5 years, 25 days ago)

2 weeks ago, I sterilized and inoculated 4 jars of rye. I used a new spore syringe and at first, I forgot to shake it. After using some milliliters I realized that and shook the syringe and used it again. Since I forgot to shake the syringe, I used a lot. I used 20 milliliters for 2 jars each 500 ml. But still it didn't help, one of my jars now have not started to colonize while the other 3 started colonizing one week ago. I'm thinking I probably got all the spores out with the first milliliters before shake.

Can I inoculate that jar that has not started to colonize now or is it better to start over? I'm worried that there will not be enough spawn for a monotub.


Extras: Filter Print Post Top
InvisibleBuckthorn
Stranger
 User Gallery
Registered: 07/25/08
Posts: 4,575
Re: Forgot to shake spore syringe [Re: Shroombie2]
    #25775972 - 01/29/19 04:52 AM (5 years, 25 days ago)

I forget to shake sometimes.


Extras: Filter Print Post Top
InvisibleErrl_Shmirl
New Kid On The Block
Male


Registered: 03/25/17
Posts: 263
Loc: Southern Plains Flag
Re: Forgot to shake spore syringe [Re: Buckthorn]
    #25776001 - 01/29/19 05:23 AM (5 years, 25 days ago)

Being that most syringes have a visible clump in them. Spores are microscopic and that clump contains an unbelievably huge amount of them, which means that throughout the rest of the syringe there are also tons of them that cant be seen. It only takes a couple three of em to start up the process we all love so much.


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 16 days, 17 hours
Trusted Cultivator
Re: Forgot to shake spore syringe [Re: Errl_Shmirl]
    #25776024 - 01/29/19 05:41 AM (5 years, 25 days ago)

First off Agar work is a *must* if you are working with grains.
Grains are not forgiving the way BRF is.
But if you insist on using spores to inoculate grain, 20ML is WAY too much dude. It will throw off the moisture content.
You should only use a single drop or two to inoculate with a syringe.

Like he said above there are countless spores in the syringe. You only need two. Not shaking didn't matter at all really

Hope this helps!


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineShroombie2
Stranger
Registered: 12/28/18
Posts: 189
Last seen: 8 months, 26 days
Re: Forgot to shake spore syringe [Re: mushpunx]
    #25776030 - 01/29/19 05:47 AM (5 years, 25 days ago)

Thanks.
That's very comforting. But I have forgot to shake the syringes before and then my BRF cakes never colonized. I will wait and see then, hopefully it will start soon.
I didn't know agar was easier to work with than spore syringes, I need to learn that soon. I also need to learn the right dosage for inoculating. But I've been observing at least that, when I increase the dosage, the colonization takes place a lot faster.


Edited by Shroombie2 (01/29/19 05:48 AM)


Extras: Filter Print Post Top
OfflineVibe_Enthusiast
Mushroom Technician
I'm a teapot


Registered: 10/16/18
Posts: 2,420
Loc: GPS signal lost.. Flag
Last seen: 1 month, 12 days
Re: Forgot to shake spore syringe [Re: Shroombie2]
    #25776061 - 01/29/19 06:22 AM (5 years, 25 days ago)

Never forget to shake it;]

You should be fine dude. Millions of spores are in there


--------------------
:greyalien:




Extras: Filter Print Post Top
OfflineVibe_Enthusiast
Mushroom Technician
I'm a teapot


Registered: 10/16/18
Posts: 2,420
Loc: GPS signal lost.. Flag
Last seen: 1 month, 12 days
Re: Forgot to shake spore syringe [Re: Shroombie2]
    #25776063 - 01/29/19 06:23 AM (5 years, 25 days ago)

Quote:

Shroombie2 said:
Thanks.
That's very comforting. But I have forgot to shake the syringes before and then my BRF cakes never colonized. I will wait and see then, hopefully it will start soon.
I didn't know agar was easier to work with than spore syringes, I need to learn that soon. I also need to learn the right dosage for inoculating. But I've been observing at least that, when I increase the dosage, the colonization takes place a lot faster.



Agar is so worth it. I wish I would have started that from the get go. All you need is a DROP... more than that can actually be worse. You may have seen faster colonization because the myc was more aggressive than the others!

Also, some spores just don't germinate. It happens


--------------------
:greyalien:




Edited by Vibe_Enthusiast (01/29/19 06:24 AM)


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 16 days, 17 hours
Trusted Cultivator
Re: Forgot to shake spore syringe [Re: Vibe_Enthusiast]
    #25776591 - 01/29/19 11:56 AM (5 years, 25 days ago)

Agar isn't necessarily easier, but it ensures a clean culture, while spore syringes are never 100% clean even with proper indoor spore printing. They frequently harbor bacteria or mold. Germinating the spores on agar gives us a 2d surface to view the culture and any contaminants that grew with it- we can then transfer a piece of clean mycelium tissue to another agar plate and end up with a clean culture.
BRF/PF tek jars typically manage OK with spore syringes but grains are much more prone to contamination.

One more thing, until you start working with agar, I find it best to inoculate a few jars with just one drop or so each. Examine the colonized jars carefully and only select the cleanest to be used for grain to grain transfer in a still air box.
Best chance of getting clean healthy spawn


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
OfflineShroombie2
Stranger
Registered: 12/28/18
Posts: 189
Last seen: 8 months, 26 days
Re: Forgot to shake spore syringe [Re: mushpunx]
    #25778324 - 01/30/19 04:18 AM (5 years, 24 days ago)

Thank,
I've never actually had contamination problems with the spore syringes and my manufacturer guarantees me that they are contamination free.


Extras: Filter Print Post Top
OfflineShroombie2
Stranger
Registered: 12/28/18
Posts: 189
Last seen: 8 months, 26 days
Re: Forgot to shake spore syringe [Re: Shroombie2]
    #25778325 - 01/30/19 04:19 AM (5 years, 24 days ago)

Should I reinoculate the jar that is not colonizing to save the rye and time for sterilization or should I start over with new jar and rye?


Edited by Shroombie2 (01/30/19 04:22 AM)


Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 16 days, 17 hours
Trusted Cultivator
Re: Forgot to shake spore syringe [Re: Shroombie2]
    #25778458 - 01/30/19 06:24 AM (5 years, 24 days ago)

Just because you haven't yet, doesn't mean you won't , because you will!
There is almost always bacteria in a spore syringe, could very well be lowering your yields

Never re inoculate,make a new one. Only use a single drop or two of spore solution


--------------------

Amateur Mycologists United
AMU Q&A


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,890
Loc: Milky way
Trusted Cultivator
Re: Forgot to shake spore syringe [Re: Shroombie2]
    #25778477 - 01/30/19 06:35 AM (5 years, 24 days ago)

Quote:

Shroombie2 said:
Thank,
I've never actually had contamination problems with the spore syringes and my manufacturer guarantees me that they are contamination free.



that's complete BS. spore prints are not axenic thus you can't make a contamination free spore syringe. there's always some level even if it's minuscule.

most syringes are made well enough they will work on PF cakes and easily get you clean growth, same for agar.

Quote:

Shroombie2 said:
Should I reinoculate the jar that is not colonizing to save the rye and time for sterilization or should I start over with new jar and rye?



you should re-make them if you inoculated with 20mL to start.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* spore syring is in trouble? mulan 14,154 12 08/29/09 09:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 5,689 4 08/11/08 03:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 7,959 2 06/01/00 08:17 PM
by 3Mshroom
* Re: Spore transfer? Anonymous 1,903 1 03/08/01 08:17 PM
by Juggles
* how far to spore prints go? AKA PC 3,750 11 01/28/03 08:07 AM
by debianlinux
* Possible Contaminated Spore Syringe? Tim2133 2,932 5 06/30/03 11:09 PM
by Sev
* spores userdoesnotexist 1,509 5 08/06/02 07:52 AM
by mickey_rourke
* How long do spore syringes last? SixCee 5,284 4 08/01/02 10:39 PM
by mickey_rourke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,919 topic views. 25 members, 158 guests and 39 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.009 seconds on 14 queries.