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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated *DELETED*
    #25770260 -

Post deleted by Kingsmanname

Reason for deletion: Dumb first time posting thread. Get rid of the whole thing.


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Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Kingsmanname] * 1
    #25770320 -

:popcorn:

So those plugs, were they sterile?


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I have left life and loves behind me, to be blown about as the sea desires, to have the freedom of the open air, and to be witness to the making of the world.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Johnny Dont]
    #25770333 -

Johnny Dont said:
:popcorn:

So those plugs, were they sterile?


Nope! But their also not straight spores, they're a little farther in the game and already turned into mycelium. So I'm hoping the wood surface that doesn't have mycelium, which would be the only thing that's not sterile, will be covered faster by the mycelium than contamination.



--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Kingsmanname] * 1
    #25770491 -

Have you grown mushrooms before?  This is a total fail.


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🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Mycolorado]
    #25770884 -

Mycolorado said:
Have you grown mushrooms before?  This is a total fail.


Can you give me some reference material that indicate's this will fail? I've tried searching similar experiments and haven't found any. I found one that mentioned, "spiking" plugs into an even less sterile version of this and had some success. So I'm not sure where you've found this info, but please provide a link of something you've found. I appreciate your input, and I assume you have experience with this since your providing info. Please link your experiment or someone elses with this so I can compare data.

Being new at this, I want data to back statements like that. Not, "similar results," or assumptions, but the comparable data. Not just speculation. Thank you. After all, I very clearly stated this is an experiment. So unless you have specific info, you can speculate all you want, but haven't given data to back your statement.

Thank you.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Kingsmanname] * 1
    #25771080 -

Mycolorado is not speculating, those jars are going to smell terrible within days and should be all the colors of the rainbow in less than 2 weeks.

Get out while you can :eek:

Or don't, just make sure to post pics :popcorn:

disregarding the fact that you've got an exotic mold farm germinating away over there, you should know;

Lions mane grows on wood.

a Lions mane substrate recipe is usually something like:

16 parts sawdust
8 parts woodchips
2 parts wheat bran
1 part gypsum
60% water

Don't experiment until you understand the basics.


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LAGM2020
LAGM2021

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Svetaketu]
    #25771092 -

Svetaketu said:
Mycolorado is not speculating, those jars are going to smell terrible within days and should be all the colors of the rainbow in less than 2 weeks.

Get out while you can :eek:

Or don't, just make sure to post pics :popcorn:

disregarding the fact that you've got an exotic mold farm germinating away over there, you should know;

Lions mane grows on wood.

a Lions mane substrate recipe is usually something like:

16 parts sawdust
8 parts woodchips
2 parts wheat bran
1 part gypsum
60% water

Don't experiment until you understand the basics.


Well, they do probably prefer those substrates, but I'm currently growing jars with almost the exact same substrate as this experiment with good results so far. So I'm not exactly worried about the "recipe.".

And as far as how the exotic mold farms turn out. I'll definitely post pics. And same thing goes to you. Any experiments or data from a same or similar experiment? You guys keep saying it's not going to work, and "contamination, blah blah." But nobody has shown any similar data, info, or pictures to show that this specific experiment will fail as you guys expect. Show me what you tested similar to this that failed! You must be speaking from a standpoint of experience. So I want your data. Pictures and links please.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated [Re: Kingsmanname] * 1
    #25771638 -

Blah, blah, blah? :facepalm:  As mentioned, LM grows on wood.  Had you put this together correctly, you might be able to use your sub mix as spawn to inoculate a sterile wood substrate.  The real issue is you inoculated a sterile substrate in a septic manner.  Every one of those jars is contaminated.  I’d bet my left nut that your other jars are contaminated as well if they were treated in the same way.  You can’t inoculate sterilized nutritious material without taking some precautions like working in front of a hepa filter or in a sab, nor can you use any tools that haven’t been sterilized and that have come into contact with open air.  As mentioned, you need to learn the basics of aseptic technique and why things are done the way they are.  No “data” is required.  I just started working with LM myself, so by no means do I claim to be an expert on the species but I don’t need to have tried what you’re doing in order to know it will fail.  Here’s a bag of sterilized sawdust that was inoculated in front of a hepa with clean spawn.  It’s getting ready to head to the fruiting chamber where it will hopefully produce actual good results. 



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🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25771659 -

This thread was moved from Mushroom Cultivation.

Reason:
wrong forum. please read the forum descriptions. Mushroom Cultivation is for all things psychedelic. Lions mane isn't psychedelic.

also your method outlined in the first post will be complete fail

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: bodhisatta]
    #25771889 -

bodhisatta said:
<img src='/forums/images/moved.gif'> This thread was moved from Mushroom Cultivation.

Reason:
wrong forum. please read the forum descriptions. Mushroom Cultivation is for all things psychedelic. Lions mane isn't psychedelic.

also your method outlined in the first post will be complete fail


Oops! My bad, I just thought it was cultivation in general. Thanks for moving it! And it might fail, I wouldn't be surprised. But we'll find out for sure.

Mycolorado, I did sterilize all my tools. And I put the plugs in in a still aired, lysol sprayed cabinet, so we'll see what happens. But if these things work. I'm coming down to Colorado to collect that left nut. I got buddies that live at Copper Mountain down there, so I'll make the trip to collect! Haha

But I understand that the likelyhood of contamination is high. My other jars were inoculated with a syringe and are just about ready for the FC. So we'll see.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25771897 -

lysol is basically useless. it is a surface sanitizer. doesn't sanitize air. surfaces go right back to being contaminated.

lysol will not make up for technique. technique will make up for using 0 lysol tho.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25772003 -



Let’s see your other jars. 


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: bodhisatta]
    #25772046 -

bodhisatta said:
lysol is basically useless. it is a surface sanitizer. doesn't sanitize air. surfaces go right back to being contaminated.

lysol will not make up for technique. technique will make up for using 0 lysol tho.


Technique! Hah Well it's a good thing I'm using a technique no one has tested! Now I'll be able to give YOU info once if fails or succeeds since you guys have never done it or ready anywhere about it either.

Although I gotta say this forum is starting to drive me nuts. You guys seriously just make new people want to leave. I get that the likely hood of failure is high. I've already acknowledged that. BUT THIS ISN'T SOMETHING I'VE FOUND INFO ON. I can post you a link to the similar article where a guy was "spiking" or pushing plug spawn into a similar substrate through a 1" verm contamination layer with the lid wide open and he had successful fruiting. So why would this even more sterile version of that be so subject to failure, without a reasonable doubt? Obviously I understand your success previously, and it's why I respect your info. But jesus fuckin christ, let me do my god damn experiments without your constant barrage of, "Everything you do is wrong," style comments. I came here for a community of positive help, especially considering it's a forum of happy loving mushrooms, but what I didn't want was people to talk down all the time. And Budhisatta, so far you've been my biggest gripe with this site. How about some helpful suggestions? Or maybe an alternative method? Or maybe devise an experiment yourself that focuses directly on non-typical inoculation of substrate using plug spawn, which is what my goal was here.

That would be more helpful to me than you constantly just saying, "Nope, this is just going to fail. You have no technique." And looking through the forum, it looks like a lot of other people share the same opinion.

Just relax a little, have a good trip, do something! I don't generally get frustrated or negative. But as a leader in a community, you have not shown good leadership so far. For future posts, either please scroll past my posts, or leave something constructive with useful information. Thank you.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Mycolorado]
    #25772054 -

Mycolorado said:


Let’s see your other jars. 




--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

Extras: Filter Print Post Top
Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25772055 -

you're not going to find info especially scientific info on how to do things blatantly wrong. That's like finding an article about how to drive your car on the rims with no tires, it's just not out there. and it's obvious without trying or needing explanation that it's dumb to anyone who knows a little bit about cars. once you know a little bit about mushrooms everything you're doing will clearly not make any sense anymore.

basic biology introduction of a non sterile item to sterilized media = fucked.

I'm building a helicopter out of cardboard and sand you got any scientific info to explain why that won't work?


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25772057 -

Thats the furthest along. 2 of my 10 haven't done anything. No contam, no mycelium. But all the other ones are fuzzy white.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

Extras: Filter Print Post Top
Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: bodhisatta]
    #25772071 -

bodhisatta said:
you're not going to find info especially scientific info on how to do things blatantly wrong. That's like finding an article about how to drive your car on the rims with no tires, it's just not out there. and it's obvious without trying or needing explanation that it's dumb to anyone who knows a little bit about cars. once you know a little bit about mushrooms everything you're doing will clearly not make any sense anymore.

basic biology introduction of a non sterile item to sterilized media = fucked.


I know. I've told you I know. The total air exposure time would be 5 seconds per plug. They were sterilized, inoculated, and sealed when shipped. So the amount of time the bag was open for and when I pulled it out of the bag is the only time it's exposed to open air contaminants. Otherwise, all media including my gloves were alcohol swabbed before pounding them into the sealed sterilized environment. And once plugged, no external contaminants can enter substrate. So if contamination is heavily present in the air, then that's the only introduced contamination possibility. If these things work, I want an apology. If they fail, I'll let you know you were right. I understand this is non-traditional and risk contamination more so, but I didn't label it "untested and experimental" for no reason.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25772078 -

Alright, dude, I tried to explain why it wouldn’t work above.  That said, plug spawn is meant to be used with unsterile substrate, namely, logs.  As I wasn’t using logs I inoculated agar with the dowels, isolated clean growth, xfer’d to sterile grain and then used it to inoculate a suitable substrate of sawdust and bran.  All this was done in front of a hepa as sterilized material readily contaminates when not handled with the utmost care. Doing shit without understanding the fundamental principles is going to result in failure. 



--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

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Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: bodhisatta]
    #25772083 -

bodhisatta said:
I'm building a helicopter out of cardboard and sand you got any scientific info to explain why that won't work?


I could definitely provide you with info that would prove why that wouldn't work. I could send you links. I could show you papers on sand density. I could show you lift capabilities of cardboard. So yes. I could give you scientific info. And I'll happily provide it if you like. But you haven't given me shit. Show me someone's contamination likely hood in open air vs a glovebox. Show me contamination rates of wood vs pf style substrate. ANYTHING. I'm asking, practically begging for this info. Not your smart-ass remarks.


--------------------
Hope for the best, expect the worse!
I want to do what no one's done yet.

Extras: Filter Print Post Top
Re: Lions Mane WBR KINGS TEK (Experimental Plug Spawn, PF Style) Untested Just Inoculated (moved) [Re: Kingsmanname]
    #25772088 -

alcohol'd gloves are no where near clean. rub your alcohol'd glove fingers on a couple petri dishes and watch the horror show.

this isn't experimentation. people throw that word around loosely. you're just doing stuff. experimentation has controls and a hypothesis. you have neither. people like to think they're experimenting.
Kingsmanname said:
bodhisatta said:
I'm building a helicopter out of cardboard and sand you got any scientific info to explain why that won't work?


I could definitely provide you with info that would prove why that wouldn't work. I could send you links. I could show you papers on sand density. I could show you lift capabilities of cardboard. So yes. I could give you scientific info. And I'll happily provide it if you like. But you haven't given me shit. Show me someone's contamination likely hood in open air vs a glovebox. Show me contamination rates of wood vs pf style substrate. ANYTHING. I'm asking, practically begging for this info. Not your smart-ass remarks.


they don't exist. show me successful murder rates using a pencil as a weapon.


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread

5g a day keeps the 5g away

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Mushrooms, Mycology and Psychedelics > Gourmet and Medicinal Mushrooms
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