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Offlineadrenalytic
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Registered: 12/16/18
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Last seen: 1 year, 2 months
BRF Tek Jar not fully colonized but pinning.
    #25744215 - 01/14/19 08:20 PM (2 years, 1 month ago)

Hey all,

First time poster and cultivator.  Been reading the site for several months now and has really helped me a lot.  I noc'd up 12 1/2 pint jars on Dec. 17th with 4 different types of spores (all p. cubensis just diff locales).  They've all been colonizing at varying rates but mostly the same except for this one type from burma.  It has a weird colonization pattern across the three jars they are in.  Anyway, one of the jars started pinning today and it's not fully colonized yet.  I'm wondering what I should do.  Birth?  Wait?  Just seeking some novice advice on the best approach.  See pic for reference.  Thanks.



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OfflineSparky101
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25744235 - 01/14/19 08:31 PM (2 years, 1 month ago)

It’s probably bacterial and started pinning due to stress. I’d wait and see if it colonizes because birthing it now will introduce the uncolonized potion to a lot more contams.

Definitely birth them away from your other jars either way and don’t try and fruit them in the same chamber as the others. Good luck!


--------------------
Never ask someone to do something that you wouldn’t do yourself.



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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: Sparky101]
    #25744242 - 01/14/19 08:35 PM (2 years, 1 month ago)

Why do you say it's probably bacterial?  I'm not trying to argue, I'm genuinely trying to understand.  I'm more inclined to think it has to do with the spores since the same growth pattern is exhibited across three different jars.  This just happens to be the first one to pin.


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OfflineSparky101
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25744492 - 01/14/19 10:39 PM (2 years, 1 month ago)

It could’ve been a dirty syringe. I only say that because of the “ring” looking area on the right side of your jar. Is that an inoculation point?


--------------------
Never ask someone to do something that you wouldn’t do yourself.



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OfflineMadValley
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Re: BRF Tek Jar not fully colonized but pinning. [Re: Sparky101]
    #25744521 - 01/14/19 10:51 PM (2 years, 1 month ago)

I had the same thing with a PE attempt on my first grow. Some of the jars colonized just fine, some showed some evidence of bacterial contam. The yellowish tint you can see in a couple places is some kind of metabolite indicating the myc is under stress. I'm going to try to fruit my problem jars directly without removing the cakes. Hopefully will get one or two fruits.


--------------------
How can you be in two places at once, when you're not anywhere at all?


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OfflineDnDRnD
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Re: BRF Tek Jar not fully colonized but pinning. [Re: MadValley]
    #25744540 - 01/14/19 11:00 PM (2 years, 1 month ago)

Now im still new myself, but what ive read is that when a culture starts pinning before fully colonized it usually indicates bacteria, contaminates, or occasionally it can just because a certain spot is overly wet or to dry


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Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: Sparky101]
    #25744600 - 01/14/19 11:32 PM (2 years, 1 month ago)

No, I marked my noc points with perm marker (or rather first sign of mycelia points).  Here are more pics.

This is all three of the "Burma" type p. cubensis jars (the pinning one on the far right).



The grow pattern is just splotchy.  No discoloration noticeable.

Even under the jars, they seem to go for the inside out as you can see colonization that doesn't attach to the outside.



And then, here is a mix of 3 of my normal jars that are progressing as expected and almost fully colonized.



I'm growing 12 in total but you get the idea.  There's three that are growing strange and 9 that are growing fine.


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OfflineMadValley
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25744620 - 01/14/19 11:52 PM (2 years, 1 month ago)

All things being equal it would point to 1) a bad syringe or 2) something different in the processing of the jars. Did they come out of the steam (or PC) together? Like, were all three at the top or bottom if you stacked them or processed differently from the other 9? Since the 3 are all the same variety, my money's on a bad syringe either contam in the needle or contam in the spore mix.


--------------------
How can you be in two places at once, when you're not anywhere at all?


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OfflineSparky101
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Re: BRF Tek Jar not fully colonized but pinning. [Re: MadValley]
    #25744665 - 01/15/19 12:19 AM (2 years, 1 month ago)

The only thing that makes sense to me is that the syringe was dirty man. I did multiple ms to grain runs with success until I emptied one into 10 jars and they all grew green.

If it were me I’d leave em be and see if they colonize completely.


--------------------
Never ask someone to do something that you wouldn’t do yourself.



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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: Sparky101]
    #25746156 - 01/15/19 08:21 PM (2 years, 1 month ago)

So here is the cake today.  Still colonizing, no discoloration or signs of contams.  Like I said earlier, it looks like the mycelium prefer to consume from the inside out.  I'm just gonna ride this out till full colonization.

Should I leave those pins alone on birth or harvest them?



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OfflineSparky101
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25747368 - 01/16/19 02:26 PM (2 years, 1 month ago)

I’d pick em off


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Never ask someone to do something that you wouldn’t do yourself.



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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: Sparky101]
    #25748580 - 01/17/19 01:09 AM (2 years, 1 month ago)

Are they still edible?


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OfflineSparky101
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25749006 - 01/17/19 10:46 AM (2 years, 1 month ago)

Yep!


--------------------
Never ask someone to do something that you wouldn’t do yourself.



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OfflineAjr12100
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25750396 - 01/18/19 12:06 AM (2 years, 1 month ago)

Imo you should wait another week and see what happens- one of my early brf to bulks  had one jar that had a massive ring of uncolonized substrate for 10 days after the other jars were 100%  but eventually it did fully colonize- and I spawned them all together  to coir and nothing went wrong

I always leave pins on at birth and just spawn them to bulk with the pins still attached


Edited by Ajr12100 (01/18/19 12:08 AM)


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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: Ajr12100]
    #25754737 - 01/19/19 11:25 PM (2 years, 1 month ago)

So here is the jar today.  It keeps fruiting but it is also colonizing and no signs of contamination (i.e., discoloration). 

My question is...what is the risk of birthing and dunking tonight?  I kind of want to eat those shrooms for a quick buzz tonight. 



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OfflineJDoom413
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25755379 - 01/20/19 08:20 AM (2 years, 1 month ago)

Quote:

adrenalytic said:
So here is the jar today.  It keeps fruiting but it is also colonizing and no signs of contamination (i.e., discoloration). 

My question is...what is the risk of birthing and dunking tonight?  I kind of want to eat those shrooms for a quick buzz tonight. 








This is a 1/2 pint jar?


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OfflineMadValley
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Re: BRF Tek Jar not fully colonized but pinning. [Re: JDoom413]
    #25755700 - 01/20/19 11:54 AM (2 years, 1 month ago)

Quote:

JDoom413 said:


This is a 1/2 pint jar?




OP so states, but you're right, in the image where OP's hand is visible it looks more like a pint wide-mouth. When I first saw them I thought they were half-pint talls. You may be onto something.


--------------------
How can you be in two places at once, when you're not anywhere at all?


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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: JDoom413]
    #25756161 - 01/20/19 03:27 PM (2 years, 1 month ago)

Yes, they are half pint talls.


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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25761711 - 01/22/19 09:34 PM (2 years, 1 month ago)

Just so y'all know, I birthed it and popped the caps, tripped mildly and had a good time.  Also dunked and rolled the cake, put it in the terrarium and currently awaiting pinning.  I'll let you know how it turns out.


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Offlineadrenalytic
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Re: BRF Tek Jar not fully colonized but pinning. [Re: adrenalytic]
    #25765852 - 01/24/19 07:26 PM (2 years, 1 month ago)

Just an update.  I birthed, dunked in the fridge overnight, and rolled in verm the following day.  Put them in the terrarium and here's the cake today.



No signs of contam.  No funky smell, just mushroom.  The pins don't look less than ordinary.  I think we'll be Ok.  Thoughts?


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