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Duggstar
Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 1 year, 3 months
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Quote:
DragonTurtle said: I've seen discrepancies with people disagreeing whether or not oyster mushrooms can grow via pf tek. Thoughts?
I'm not big into cultivation, I'm just getting back into it after a long gap, but I've seen oysters fruiting off BRF cakes, so there's your answer...
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DragonTurtle
Stranger
Registered: 08/24/11
Posts: 91
Last seen: 2 years, 3 months
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Re: Potential oyster mushroom [Re: Duggstar]
#25745526 - 01/15/19 01:21 PM (5 years, 2 months ago) |
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Yeah, yeah, yes, yes! Right on buddy, much love my dude!
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DragonTurtle
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When you say clean it up using agar plate can you elaborate on what you mean by that?
I assume that you're referring to contaminant particulates and so on(but maybe you're talking about strain genetics as far as isolation goes?). The only spore syringes that I've ever made I've made from a spore print that I collected in the field.
The idea of the lab experiment would be for the student to create a spore syringe from scratch. So they have to sterilize the syringe they have to perform the scraping and so on and so forth of the spore print in sterile conditions (which we have hoods and stuff in the lab so it wouldn't be difficult I expect)
given a 10 to 12 cc syringe I assume that each lab group / student would be able to inoculate three to four brf jars. if the spore syringe making and the inoculation was all performed under sterile conditions I assumed that the success rate would be at least allowable for a lab experiment like this.
I don't know man I've you know done a lot from scratch in the field and all that so if we just handed the students a liquid culture and said go for it thay kind of defeat the purpose in my opinion, maybe takes the love away from the process of doing it, but I'm still needing insight on what you guys might think about something like this.
What's the funnest way to start someone out in mycology? for me obviously the hunt for psychedelic mushrooms and so on has birthed into this love of medicinal mushrooms and edible mushrooms combined with my chemistry background excetera excetera
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Duggstar
Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 1 year, 3 months
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It's probably a question that would be better answered on the cultivation forum, I'm by no means an expert, but primarily I mean to isolate the mycelium away from contams. Yes, of course, you can isolate a strain in the same way, but you would have to take it through to fruiting stage to see which strain works best. When you collect a spore print from a wild mushroom, there's gonna be contams in the spore print itself because it's fruited in open air, so no matter how sterile your lab conditions are there's still gonna be a high risk of contamination unless you isolate it on agar first, and that's gonna take at least one transfer on agar. The other option is to clone it. If you rip the mushroom open to expose some clean flesh, cut a little bit off, and put it on agar and let the mycelium grow out, and then transfer it to another plate.
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DragonTurtle
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Re: Potential oyster mushroom [Re: Duggstar]
#25745817 - 01/15/19 03:29 PM (5 years, 2 months ago) |
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Ah yes,I am a little rusty on methodologies and so on.
Good information thanks buddy!
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DragonTurtle
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Assumed coprinus sp?
No clue
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doctorghosty
is the name of me
Registered: 09/02/10
Posts: 11,420
Loc: North GA, God's fav
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Psathyrella and Hypholoma lateritium
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DragonTurtle
Stranger
Registered: 08/24/11
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Last seen: 2 years, 3 months
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Awesome. Thanks a lot doc, I'm definitely seeing other hypholoma species around my area, will update!
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DragonTurtle
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Registered: 08/24/11
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Last seen: 2 years, 3 months
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Weird fungus growing from rotten plywood roof
Is this distinguishable as an oyster mushroom species?
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Pluviophile
Stranger
Registered: 10/26/17
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Loc: Massachusetts
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First 2 pics looks like Peziza sp.
Second 2 are probably old rotten Pleurotus.
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DragonTurtle
Stranger
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Re: Potential oyster mushroom [Re: Pluviophile]
#25757286 - 01/20/19 08:38 PM (5 years, 2 months ago) |
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Awesome thanks 👍
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Doc9151
Mycologist
Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 8 months
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Re: Potential oyster mushroom [Re: Pluviophile]
#25758509 - 01/21/19 12:34 PM (5 years, 2 months ago) |
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Quote:
Pluviophile said: First 2 pics looks like Peziza sp.
Second 2 are probably old rotten Pleurotus.
I agree with
-------------------- Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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DragonTurtle
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Re: Potential oyster mushroom [Re: Duggstar]
#25770065 - 01/26/19 01:01 PM (5 years, 2 months ago) |
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Wow!
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DragonTurtle
Stranger
Registered: 08/24/11
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Are these dead man's fingers?
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DragonTurtle
Stranger
Registered: 08/24/11
Posts: 91
Last seen: 2 years, 3 months
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Somebody respond please
Looking for xylaria polymorpha in the photo above
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Doc9151
Mycologist
Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 8 months
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Quote:
DragonTurtle said: Are these dead man's fingers?
It could be but I can't see it well enough to make an accurate identification.
-------------------- Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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DragonTurtle
Stranger
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Re: Potential oyster mushroom [Re: Doc9151]
#25827817 - 02/21/19 01:36 PM (5 years, 1 month ago) |
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These were just a log over from where I found P. Cervinus, the salmon color in the gills makes me think it is the same mushroom.
And these are new to me, coming from what looks like monkey grass clippings.
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Duggstar
Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 1 year, 3 months
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Looks like Pluteus sp. and Bolbitius titubans.
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DragonTurtle
Stranger
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Last seen: 2 years, 3 months
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Re: Potential oyster mushroom [Re: Duggstar]
#25870837 - 03/12/19 11:23 PM (5 years, 17 days ago) |
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Anyone got a scientific name for this?
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Moria841
Registered: 07/02/18
Posts: 4,970
Loc: NJ
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Looks like a sporulated Calvatia Cyathiformis
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