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Offlinexwave3000
enthusiast

Registered: 02/27/03
Posts: 287
Last seen: 8 years, 27 days
Cloning question
    #2573503 - 04/18/04 12:00 PM (13 years, 2 months ago)

Cloning teks tell you to douse your mushroom tissue in hydrogen peroxide. Could i use alcohol diluted with water instead?


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InvisibleLoki
fluid druid
 User Gallery

Registered: 02/10/04
Posts: 15,467
Loc: Zone ate
Re: Cloning question [Re: xwave3000]
    #2573512 - 04/18/04 12:06 PM (13 years, 2 months ago)

Nope, h2o2 is better for this job, as it evaporates
leaving only h2o and o2, were as alcohol kills all the tissue it
soaks into.

Stick to h2o2 for this task.


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OfflineNoG
journeyman

Registered: 04/15/04
Posts: 98
Last seen: 12 years, 4 months
Re: Cloning question [Re: Loki]
    #2573543 - 04/18/04 12:19 PM (13 years, 2 months ago)

+ alcohol is not a sterilizer

alchohol can clean but it doesnt kill all the things hydrogen peroxide does.


Edited by NoG (04/18/04 12:20 PM)


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OfflineThatDaltonGuy
PermanentlyFried
Registered: 04/07/04
Posts: 213
Loc: USA
Last seen: 13 years, 1 month
Re: Cloning question [Re: xwave3000]
    #2573558 - 04/18/04 12:26 PM (13 years, 2 months ago)

yeah h202 kicks ass...i tried cloning mycelium and dunked it in alchohol... thing never even molded... yet it never grew either =/
h202 is my best friend =)

h202 is what you would use all round on sterilizing the walls of ur terrarium and everything pretty much having to do with "living conditions" of the mushrooms...

alchohol is what u use to sterilize the active moving things such as glass prods or a knife or something that u could dip and flame... usualy stuff used to put spores into water for making syringe... or something to do with moving spores around...


--------------------
Let me remind you that the above text is completely false and has been typed to you by an escaped mental patient and shouldn't be taken seriously unless you yourself also have a mental condition. These kind of things need to be taken into consideration by those viewing these threads.


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: Cloning question [Re: ThatDaltonGuy]
    #2574463 - 04/18/04 05:21 PM (13 years, 2 months ago)

h2o2 doesn't 'sterilise' anything...

It kills spores and is useful for protecting against airborne contamination as it will stop any stray spores that enter from germinating.

I sometimes clone mushroom tissue directly to honey-water by 1st dunking for a few secs in straight 3% h2o2 and then immediately transferring to prepared honey-water (also treated with h2o2 - 3% to volume)

This has been quite successful and my contam rate is practically zero.


--------------------
Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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OfflineJohnnyJ
Junior VicePresident

Registered: 04/14/04
Posts: 63
Last seen: 7 years, 6 months
Re: Cloning question [Re: xwave3000]
    #2574503 - 04/18/04 05:37 PM (13 years, 2 months ago)

When using the standard pf tek, could you put your dry layer of vermiculite in, with a h2o2 sprayed layer of vermiculite on top of that?
Would that help?
JohnnyJ


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OfflineNoG
journeyman

Registered: 04/15/04
Posts: 98
Last seen: 12 years, 4 months
Re: Cloning question [Re: JohnnyJ]
    #2574746 - 04/18/04 08:33 PM (13 years, 2 months ago)

Hydrogen Peroxide (H2O2) is a clear sharp smelling substance very similar in appearance to water (H2O). Like water it is made up of Hydrogen (H2) and Oxygen (O2), however H2O2 has an extra Oxygen atom in an unstable arrangement. It is this extra atom that gives H2O2 its useful properties. H2O2 has been used for many purposes including cleaning, bleaching, sterilizing, rocket fuel, animal feed treatment and lots of other things.

h202 is a steriliser believe me.


Later NoG


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