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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Signs of Bacteria in Grain Spawn
    #25734500 -

I've done a bunch of searching and still have trouble identifying when cube grain spawn is completely clean, slightly bacterial, or more severely effected.  Discoloration is easy, but what about when the mycelium just grows "weird."  There are a lot of responses to peoples pictures saying its bacterial without real explanations as to why that is the opinion.

Maybe a kind soul out there has pics of perfect grain spawn and slightly bacterial examples?  I use mostly oats and some rye. 

Or even better, a previous deep discussion on the topic (with pictures).


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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25734514 -

There's a thread full of contam examples  UTSF. For a practical approach if the grain isn't dry but growth becomes slow or stagnant with no visible mold it's probably bacterial.


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Never give up, never surrender.

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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25734535 -

Here you go!

https://www.shroomery.org/forums/showflat.php/Number/17231150/fpart/1/vc/1

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Re: Signs of Bacteria in Grain Spawn [Re: USMiro]
    #25734576 -

Thats a great thread, but not exactly what I'm talking about.  Its easy to know if you have bacterial contamination when there are uncolonized areas of jar, excessive liquids, or different colors.  When you have nice white 100% colonized grains, but something is still wrong, those are the tricky ones.


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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25734582 -

Usually sweat on the glass... condensation looks a little different... can develop a thickness to it, look slimy: all the while completely colonized


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šŸ„šŸ’ššŸ˜Ž

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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25734594 -

Healthy mycelium is sorta transparent and lighter, u can sorta see the color of the grain through the mycelium.

Bacterial mycelium is thick and foamy looking, very dense and bright white.

But it's hard to kmow the difference till uve actually seen clean spawn. I don't have pics sorry, I'm sure someone does.

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Re: Signs of Bacteria in Grain Spawn [Re: nube424]
    #25734601 -

It's rare that there's NO bacteria


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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25734694 -

Caps McGee said:
It's rare that there's NO bacteria


That's just it. It's more identifying the degrees of bacterial.

You learn to recognize the clean look after you've seen it put out several good flushes before it goes to shit.

Super bacterial will look kinda thick and will contaminate after the first flush or two.

If you shake your jar and it recovers slow (more than 24hrs) or grows back different looking, than it's so bactetial you should throw it out.

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Re: Signs of Bacteria in Grain Spawn [Re: Subfinder]
    #25735563 -

Came across this while continuing the search.

https://www.shroomery.org/forums/showflat.php/Number/23662280

Always appreciative of folks spreading their knowledge in a well presented way.

I'm gonna post some jar pics soon to get some opinions.  Thanks in advance.


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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25735571 -

Real trouble is them hidden contams


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Never give up, never surrender.

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Re: Signs of Bacteria in Grain Spawn [Re: Failboat]
    #25735612 -

If ur trying to avoid bacteria, wash ur grains WELL b4 hydrating. Pc longer than normal. I do 2 hrs. 1.5 hrs I end up getting some bacteria, my pc is probly fucked up, and clean ur cultures on agar.

U'll never completely avoid bacteria, it's everywhere. But when it outnumbers other microbes, that's what's called a bacterial infection.

Think like vagina (sorry ladies). There's always bacteria, but when there's a high number if particular bacteria it causes infection. Basically the same thing here.

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Re: Signs of Bacteria in Grain Spawn [Re: nube424] * 1
    #25735904 -

Mushrooms and vagina... maybe it's bacteria I like so much?


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šŸ„šŸ’ššŸ˜Ž

Ask Caps! (My Q&A Thread)
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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25735910 -

Mmmmm bacteria :begger: can I have some? Lol

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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25735912 -

Quote:
If ur trying to avoid bacteria, wash ur grains WELL b4 hydrating


Why?


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Signs of Bacteria in Grain Spawn [Re: van hatton]
    #25735936 -

Well I use wbs and it's dirty as fuck. So I wash it real good to get what I can off it.

U don't wash ur grains b4 u cook them?

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Re: Signs of Bacteria in Grain Spawn [Re: nube424]
    #25735940 -

Not oats... personally


--------------------
šŸ„šŸ’ššŸ˜Ž

Ask Caps! (My Q&A Thread)
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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25735990 -

Well I don't use oats. Maybe they come pretty clean already. Wbs tho, it's dirty as fuck and needs to be washed.

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Re: Signs of Bacteria in Grain Spawn [Re: nube424]
    #25736030 -

Come over to the oat side...


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Never give up, never surrender.

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Re: Signs of Bacteria in Grain Spawn [Re: Failboat]
    #25736058 -

Haha Idk man, I do wbs with my eyes closed now. It would feel weird to switch. I stick with what I know.

Everything I need to grow shrooms is in the same plaza :rockon: God bless America!

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Re: Signs of Bacteria in Grain Spawn [Re: nube424]
    #25736543 -

Here are a few jars that I suspect are bacterial and/or contaminated.  The wispy growth inbetween the the whiter growth is a bit concerning.  Have a look and let me know. 





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Re: Signs of Bacteria in Grain Spawn [Re: Failboat]
    #25736561 -

Quirkmeister92 said:
Come over to the oat side...


Lmao I think you told me this a a few months back, maybe Iā€™m thinking of a different person? :lol: anyway, yeah oats rock

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Re: Signs of Bacteria in Grain Spawn [Re: nube424]
    #25736575 -

nube424 said:
Well I use wbs and it's dirty as fuck. So I wash it real good to get what I can off it.

U don't wash ur grains b4 u cook them?


But what's it being dirty have Todo with bacteria.

How would washing before hydrating prevent bacteria.i don't understand.


My wheats probably dirtier than your wbs.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25736577 -

They are yes... you're looking for that type of sweat on the jar, and the bright white, creamy looking mycelium; especially prevalent in the bottom of the right jar at the bottom... may also be mold on the left side center of that same jar... matter fact, pretty sure both of those jars have a grey mold of some sort, all 5 are bacterial, but I can't see any obvious mold in the first 3... not that it isn't there, just from the photo... are you using a SAB? Pressure cooker? Agar?


--------------------
šŸ„šŸ’ššŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25736692 -

Well, its good to know I can at least identify there is a problem.  I did everything by the book.  Everything in a SAB.  Spores to agar, transfered  to new plates. Innoc'd with clean plates.  Hydrated and Pc'd grain jars with Bods oak tek.

I had a fairly high amount of contamination in my agar work, that I threw out.  And that can't be attributed to anything else but fucking up in the SAB.  So I am leaning towards there is where improvements are needed.  I wipe everything with iso, spray walls with soapy water, wipe everything with iso that goes in, flame every time I use a scalpel, no hands above plates or jars, etcā€¦.

There are some success stories (at least I hope). I grabbed a few random jars out of my good egg pile.  They look ok?






I also put some Tamps and ATL's on rye, and those all look nice and clean.  No contams or problems thereā€¦.Maybe my oat prep is off?


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Edited by rickyswamps (01/10/19 06:17 PM)

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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25736712 -

Are you waiting 10 minutes after setup to begin? Elevating your plates? Using slow concise movements? I only wash my hands to the elbows with antibacterial soap and warm water, and I work my SAB dry... no gloves, no iso... I don't get many satellites, typically contamination only occurs in germ or tissue plates, and not always then...


--------------------
šŸ„šŸ’ššŸ˜Ž

Ask Caps! (My Q&A Thread)
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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25736753 -

Yep, I wait a while to start work and plates are off the floor elevated. I wear a long sleeve shirt with gloves.  I'm working on the movements, but am at least aware of what I'm supposed to do.  A few mistakes are made with cutting wedges and what not.  I definitely don't use anything I accidentally drop or touch a non sterile surface.

One possibility is that I inoculated with some whole plates or half plates.  Read somewhere about it being better to not use any of the agar that is towards the edge because of higher possibility of contams on the walls of petri.

Another possibility,  I use the SAB with wet paper towels as the floor.  The lid isn't flat and can't be used.  I know some people use wet towels, don't see much of a difference between the two.

Whats the word on that second set of spawn jars?  Big ups for the help.


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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25736763 -

I'd spawn them, still a bit of bacteria here and there, but nothing that concerns me... try drying your oats overnight


--------------------
šŸ„šŸ’ššŸ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: Signs of Bacteria in Grain Spawn [Re: Caps McGee]
    #25736769 -

Trying to explain your sab technique with words is like trying to melt steel with a lighter. Doesn't work well.


Make a video so we can judge you :oldman: :lol:

If u don't wanna post it you can at least review it.
I like taking the video overtop my work. So if anything goes over the media I'll see it.


:bongload:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Signs of Bacteria in Grain Spawn [Re: Thesunbeam]
    #25736784 -

Thesunbeam said:
Quirkmeister92 said:
Come over to the oat side...


Lmao I think you told me this a a few months back, maybe Iā€™m thinking of a different person? :lol: anyway, yeah oats rock


That was me.

Rickyswamps
Are you venting your PC properly? Do you wipe your SAB with soap water? I would stop putting the wet paper towels on the floor, just be sure to clean the surface well beforehand. Are you reusing blades? I use a fresh sterile blade for each transfer and I sterilize the handle in the PC.


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Never give up, never surrender.

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Re: Signs of Bacteria in Grain Spawn [Re: Failboat]
    #25736819 -

PC vents for 10 minutes before putting weight on.  Then pc'd for 2 hours.

Inside of SAB is sprayed with soapy water.  Definitely reusing my blade, with a flame in-between. It's an x-acto, so can't just throw those puppies out.  Sterilizing the handle is a great idea though.  I wipe it down completely with ISO before use.


--------------------
:sporedrop:Unmodded, Unflipped, No Drill, Monotub TEK:sporedrop:

:stoned:OTD - Let's Talk Music:stoned:

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Re: Signs of Bacteria in Grain Spawn [Re: van hatton]
    #25736831 -

van hatton said:
Trying to explain your sab technique with words is like trying to melt steel with a lighter. Doesn't work well.


Make a video so we can judge you :oldman: :lol:

If u don't wanna post it you can at least review it.
I like taking the video overtop my work. So if anything goes over the media I'll see it.


:bongload:


I need that cane smacking smiley in real life.  Maybe the girl would help train me.


--------------------
:sporedrop:Unmodded, Unflipped, No Drill, Monotub TEK:sporedrop:

:stoned:OTD - Let's Talk Music:stoned:

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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25736862 -

rickyswamps said:
PC vents for 10 minutes before putting weight on.  Then pc'd for 2 hours.

Inside of SAB is sprayed with soapy water.  Definitely reusing my blade, with a flame in-between. It's an x-acto, so can't just throw those puppies out.  Sterilizing the handle is a great idea though.  I wipe it down completely with ISO before use.


Just buy 2. Scapels and 100 blades you'll never run out. Plus u can flame the handle :awesome:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Signs of Bacteria in Grain Spawn [Re: van hatton]
    #25736979 -

Don't vent for 10 minutes, vent for AT LEAST 10 minutes. You need a steady stream of steam, no air, coming out before you put the rocker on.

The point is expelling all the air and replacing it with steam. If you don't have a steady stream then you obviously haven't expelled all the air.


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Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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Re: Signs of Bacteria in Grain Spawn [Re: 36fuckin5]
    #25737079 -

11 minutes?  :tryingnottodie:


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Re: Signs of Bacteria in Grain Spawn [Re: rickyswamps]
    #25739797 -

I can't help but observe that my rye jars have 0 signs of bacterial issues, and have problems in half of my oat jars.

I read through this:
https://www.shroomery.org/forums/showflat.php/Number/24655182/fpart/1/vc/1


I'm gonna prepare some more oat jars with the following changes:
1.  lids with SFDs instead of poly
2. rinse the oats before boiling
3. dry oats super well before loading jars
4. increase my vent time in PC

I'm about to do some G2G with what good jars I have and am nervous about having more contam issues in the transfer.  I have more rye too, gonna do G2G onto rye as well to see how it goes.  Im guess there is no issue with using an oats master to innoc rye grain.


--------------------
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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