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OfflineGreeneT
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I Need some advice on Contams.. Ive gotten for the third time Please Help
    #25731802 - 01/08/19 04:36 PM (2 years, 1 month ago)

My Fellow Mycologist, Ive just recently discoverd mycology and Im Highly Interested in this Field, Ive tried to inoculate some LME Agar plates that I made and For the 3rd time ive gotten what seems to me Mold some kind of white mold. I inculated twice in a SAB the first time with just open holes. Then the second time. I added gloves to it so it could be a closed off glove box and got the same results. Im hoping one of you,my fellow Mycologists, could give me some tips or  insight. Thanks url=https://files.shroomery.org/files/19-002/698322877-20190108_162908


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OfflineGreeneT
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25731810 - 01/08/19 04:40 PM (2 years, 1 month ago)



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OfflineGreeneT
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25731814 - 01/08/19 04:41 PM (2 years, 1 month ago)

This is what my Plates have been looking like after a fews days after inoculating i still have agar that was made and it hasnt gotten any contams yet


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OfflineNaturalelixir
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25731823 - 01/08/19 04:47 PM (2 years, 1 month ago)

Definitely ditch the glovebox its doing more harm then good, stick with the SAB and make sure everything is correct(Time in the PC, Your Technique, etc.) and try again. Patience is a virtue in this hobby.


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OfflineGreeneT
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Naturalelixir]
    #25731980 - 01/08/19 06:20 PM (2 years, 1 month ago)

Thanks Bro I Really Appreciate The Wisdom ✊


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Offlinezxcv101
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25731988 - 01/08/19 06:28 PM (2 years, 1 month ago)

What are you inoculating the dishes with? This is what happens to me if I wait to long to transfer a wild print.


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OfflineGreeneT
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: zxcv101]
    #25733954 - 01/09/19 03:27 PM (2 years, 1 month ago)

A LC syringe to inoculate


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OfflineBlacktruffle27
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25733961 - 01/09/19 03:31 PM (2 years, 1 month ago)

Looks like mycelium to me. Try a few transfer and see what happens. Mine looked like this after lc. Did a few transfers and bam, nice growing mycelium.


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OfflineGreeneT
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Naturalelixir]
    #25733963 - 01/09/19 03:32 PM (2 years, 1 month ago)

I had another question as well, My SAB is about 60 quarts, and i read that using a bigger tub like a 105qt, would help displace the air while working in it, Does any one know if this would help?


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OfflineBlacktruffle27
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25733964 - 01/09/19 03:32 PM (2 years, 1 month ago)

Think its because mycelium is scattered everywhere and not just in the centre of the plate


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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25733966 - 01/09/19 03:33 PM (2 years, 1 month ago)

I appreciate that i was in doubt bc i have never seen any mycelium to look like that, Im going to get on that asap Thanks Bro


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OfflineBlacktruffle27
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25733969 - 01/09/19 03:34 PM (2 years, 1 month ago)

Ill post a picture of my similar plate when I get home, and the one after 1 transfer


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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25733974 - 01/09/19 03:36 PM (2 years, 1 month ago)

Great, Ill keep a look out for them


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OfflineBlacktruffle27
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25734004 - 01/09/19 03:55 PM (2 years, 1 month ago)

Wouldnt hurt to try a few transfers. Go for those bits near the middle. Tiny cuts and put dead centre of new plates. Flame your knife between each transfer and cool in recieving plate.
Hopefully I am right and you'll see a different growing pattern this time.


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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25734145 - 01/09/19 05:24 PM (2 years, 1 month ago)

I appreciate the Wisdom Bro


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OfflineBlacktruffle27
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25734346 - 01/09/19 06:59 PM (2 years, 1 month ago)

Here you go

First pic is lc to agar
Second pick is first transfer
Third pic still waiting for 2nd transfer to grow.
Will update when its ready

No worries matey


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OfflineGreeneT
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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25734488 - 01/09/19 08:08 PM (2 years, 1 month ago)

Hell Yeah Man Thats AWESOME!!! ,Now I really have some Faith, Keep up your work too Bro✊, Ill post some pics as well Soon


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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
    #25760969 - 01/22/19 03:46 PM (2 years, 1 month ago)

So any updates on those transfers?


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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25765114 - 01/24/19 12:05 PM (2 years, 1 month ago)

I had to restart bc I tossed everything before we last spoke,I just did my first tranfer yesterday from my first lc plate so I will update in a few days


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Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
    #25765577 - 01/24/19 04:25 PM (2 years, 1 month ago)

This is the fist plate fron the LC syringe
files.shroomery.org/files/19-004/836495988-20190124_161734.jpg][/url]


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