|
 GreeneT
Wisdom Seeker
 
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
 I Need some advice on Contams.. Ive gotten for the third time Please Help 
#25731802 - 01/08/19 04:36 PM (2 years, 1 month ago) |
|
|
 My Fellow Mycologist, Ive just recently discoverd mycology and Im Highly Interested in this Field, Ive tried to inoculate some LME Agar plates that I made and For the 3rd time ive gotten what seems to me Mold some kind of white mold. I inculated twice in a SAB the first time with just open holes. Then the second time. I added gloves to it so it could be a closed off glove box and got the same results. Im hoping one of you,my fellow Mycologists, could give me some tips or insight. Thanks url=https://files.shroomery.org/files/19-002/698322877-20190108_162908
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
 Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25731810 - 01/08/19 04:40 PM (2 years, 1 month ago) |
|
|
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25731814 - 01/08/19 04:41 PM (2 years, 1 month ago) |
|
|
This is what my Plates have been looking like after a fews days after inoculating i still have agar that was made and it hasnt gotten any contams yet
|
Naturalelixir
Backyard Mycologist
Registered: 03/20/18
Posts: 165
Loc: USA 
Last seen: 3 months, 8 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25731823 - 01/08/19 04:47 PM (2 years, 1 month ago) |
|
|
Definitely ditch the glovebox its doing more harm then good, stick with the SAB and make sure everything is correct(Time in the PC, Your Technique, etc.) and try again. Patience is a virtue in this hobby.
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Naturalelixir]
#25731980 - 01/08/19 06:20 PM (2 years, 1 month ago) |
|
|
Thanks Bro I Really Appreciate The Wisdom ✊
|
zxcv101
Stranger
Registered: 07/09/09
Posts: 64
Loc: SW
Last seen: 1 year, 1 month
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25731988 - 01/08/19 06:28 PM (2 years, 1 month ago) |
|
|
What are you inoculating the dishes with? This is what happens to me if I wait to long to transfer a wild print.
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: zxcv101]
#25733954 - 01/09/19 03:27 PM (2 years, 1 month ago) |
|
|
A LC syringe to inoculate
|
Blacktruffle27
WavyCaps
Registered: 10/23/18
Posts: 135
Loc: uk
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25733961 - 01/09/19 03:31 PM (2 years, 1 month ago) |
|
|
Looks like mycelium to me. Try a few transfer and see what happens. Mine looked like this after lc. Did a few transfers and bam, nice growing mycelium.
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Naturalelixir]
#25733963 - 01/09/19 03:32 PM (2 years, 1 month ago) |
|
|
I had another question as well, My SAB is about 60 quarts, and i read that using a bigger tub like a 105qt, would help displace the air while working in it, Does any one know if this would help?
|
Blacktruffle27
WavyCaps
Registered: 10/23/18
Posts: 135
Loc: uk
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25733964 - 01/09/19 03:32 PM (2 years, 1 month ago) |
|
|
Think its because mycelium is scattered everywhere and not just in the centre of the plate
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25733966 - 01/09/19 03:33 PM (2 years, 1 month ago) |
|
|
I appreciate that i was in doubt bc i have never seen any mycelium to look like that, Im going to get on that asap Thanks Bro
|
Blacktruffle27
WavyCaps
Registered: 10/23/18
Posts: 135
Loc: uk
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25733969 - 01/09/19 03:34 PM (2 years, 1 month ago) |
|
|
Ill post a picture of my similar plate when I get home, and the one after 1 transfer
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25733974 - 01/09/19 03:36 PM (2 years, 1 month ago) |
|
|
Great, Ill keep a look out for them
|
Blacktruffle27
WavyCaps
Registered: 10/23/18
Posts: 135
Loc: uk
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25734004 - 01/09/19 03:55 PM (2 years, 1 month ago) |
|
|
Wouldnt hurt to try a few transfers. Go for those bits near the middle. Tiny cuts and put dead centre of new plates. Flame your knife between each transfer and cool in recieving plate.
Hopefully I am right and you'll see a different growing pattern this time.
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25734145 - 01/09/19 05:24 PM (2 years, 1 month ago) |
|
|
I appreciate the Wisdom Bro
|
Blacktruffle27
WavyCaps
Registered: 10/23/18
Posts: 135
Loc: uk
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25734346 - 01/09/19 06:59 PM (2 years, 1 month ago) |
|
|
Here you go
 
First pic is lc to agar
Second pick is first transfer
Third pic still waiting for 2nd transfer to grow.
Will update when its ready
No worries matey
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25734488 - 01/09/19 08:08 PM (2 years, 1 month ago) |
|
|
Hell Yeah Man Thats AWESOME!!! ,Now I really have some Faith, Keep up your work too Bro✊, Ill post some pics as well Soon
|
Blacktruffle27
WavyCaps
Registered: 10/23/18
Posts: 135
Loc: uk
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: GreeneT]
#25760969 - 01/22/19 03:46 PM (2 years, 1 month ago) |
|
|
So any updates on those transfers?
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25765114 - 01/24/19 12:05 PM (2 years, 1 month ago) |
|
|
I had to restart bc I tossed everything before we last spoke,I just did my first tranfer yesterday from my first lc plate so I will update in a few days
|
GreeneT
Wisdom Seeker
Registered: 11/15/18
Posts: 13
Last seen: 1 month, 5 days
|
Re: I Need some advice on Contams.. Ive gotten for the third time Please Help [Re: Blacktruffle27]
#25765577 - 01/24/19 04:25 PM (2 years, 1 month ago) |
|
|
This is the fist plate fron the LC syringe
files.shroomery.org/files/19-004/836495988-20190124_161734.jpg] [/url]
|
|
Threaded
Previous
Index
Next
Edit 
Reply 
