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 NatureBoy638
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 Quick agar question 
#25731242 - 01/08/19 12:00 PM (2 years, 1 month ago) |
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Hello all, might try making agar for the first time today but I am wondering, how many times do I have to PC? Is it okay to just PC my playes after I have made my agar and poured them? Or should I always PC the plates and agar and the pour inside a SAB? Also can potato water be a substitute for distilled water or can tap water work for making agar?
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 Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,356
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Plastic petris will melt in the pc... you can do no pour agar where you pour and then PC, but need proper containers... to pour petris, PC once after mixing at 15psi for 30 minutes, and pour in a SAB... let them cool and wrap them if you're not using them immediately... tap water works fine, you're sterilizing it... need to search, find a tek, and get an idea of what you're doing before you get started... what type of dishes are you using?
RESEARCH! We're glad to help, but questions like this can be answered faster using the search function
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NatureBoy638
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Okay I do have an idea of what I would like to do since I have many jars, but I also have a few pasty plates and needed confirmation. Can I also wipe the red hot xacto knife onto a sanitizer wipe? Saw some people do alcohol wipes but this is the best I can do I think. also are you saying plastic petris can't be used at all or they can be PCd if they have the agar in them?
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zxcv101
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Wiping your red hot exact knife on an alcohol pad defeats the purpose. Once it touches that pad it is no longer sterile. Alcohol does not sterilize. Getting it red hot does.
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Caps McGee
Grandaddy Smurfshack
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Flaming sterilizes, sanitizer wipes only sanitize... it'd be like showering and then wiping shit on your face... slice the receiving plate or just hold the scalpel in the SAB for 10 seconds before use to cool
Plastic petris will melt in the PC, you have to pc the agar in a vessel and pour them in a SAB...
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NatureBoy638
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okay, that can be for my roommate then lol. anyway I am seeing that you can PC the pasty plates but you just said I shouldn't PC plastic so what's good? I appreciate all the feedback
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What up habibi you've reached my signature and I haven't found shit to put in it yet, congrats.
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Caps McGee
Grandaddy Smurfshack
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Man... search function... plastic petris are not pp5... pp5 will survive the heat, pasty plate containers are pp5... I prefer pouring; less condensation and makes me feel like a scientist
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NatureBoy638
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Re: Quick agar question [Re: zxcv101]
#25731293 - 01/08/19 12:26 PM (2 years, 1 month ago) |
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thanks, so can I still PC glad mini rounds? and would honey work for agar or pure cane sugar granules work better?
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What up habibi you've reached my signature and I haven't found shit to put in it yet, congrats.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
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Search function... mini rounds are the container of choice... honey and sugar rarely turn out well... especially in no pours
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NatureBoy638
Stranger
Registered: 11/08/17
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Loc: Ga
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I am! You don't know how much I have been looking around for some time now, but also really appreciate someone proof reading my thoughts, thank you very much.
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What up habibi you've reached my signature and I haven't found shit to put in it yet, congrats.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,356
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NatureBoy638
Stranger
Registered: 11/08/17
Posts: 115
Loc: Ga
Last seen: 6 months, 11 days
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 Just trying to communicate with another person thanks for trying to teach me something anyway. would've been nicer to link me to this though https://www.shroomery.org/forums/showflat.php/Number/21922023
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What up habibi you've reached my signature and I haven't found shit to put in it yet, congrats.
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mushboy
Moooshboy?
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that link is within the link caps sent you. dont be such a wiener.
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NatureBoy638
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Re: Quick agar question [Re: mushboy]
#25731335 - 01/08/19 12:47 PM (2 years, 1 month ago) |
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ugh, not the way I wanted to come off. but if you are taking the time to respond then just answer my question instead of referring me to pages I've already read and do not understand. Thank you
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What up habibi you've reached my signature and I haven't found shit to put in it yet, congrats.
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mushboy
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you asked multiple basic as f questions. ...that were answered
wait: yes you can use tap water. now they have all been answered.
Edited by mushboy (01/08/19 12:55 PM)
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NatureBoy638
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Re: Quick agar question [Re: mushboy]
#25731353 - 01/08/19 12:57 PM (2 years, 1 month ago) |
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you forgot my question about honey and pure cane sugar lol! the tap water question was already answered by caps, and im sorry if they are basic af questions I said it was my first time lol. Seems like every 5 mins I spend on this site I have another question
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What up habibi you've reached my signature and I haven't found shit to put in it yet, congrats.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
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Re: Quick agar question [Re: mushboy]
#25731364 - 01/08/19 01:00 PM (2 years, 1 month ago) |
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I answered that one too... the link you posted is the exact same as i posted... don't be a douche, and don't take advantage of other's kindness... I answered basic questions that had you actually been researching, would've not needed to ask... I answered all your questions, and provided you with a list of links on everything you need, from a trustworthy source, and I'm a dick? Grow up
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mushboy
Moooshboy?
Registered: 04/24/05
Posts: 24,722
Loc: better trashcan
Last seen: 23 minutes, 41 seconds
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caps covered that sugar shit.
every question you have has been thought up, asked and already argued about 100x over on this site every day.
using the search feature and using the link sent to you is the best way to answer your questions. thats how most of us learned.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,356
Loc: ally known as ...
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Also
Quote:
Caps McGee said:
honey and sugar rarely turn out well... especially in no pours
You're not even reading the answers I'm giving you...
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,356
Loc: ally known as ...
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The point isn't that you don't know, the point is that the information is here multiple times over... it's going to take more effort than someone spoonfeeding you... ya dig?
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