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OfflineJillsa Goodwitch
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Registered: 10/23/18
Posts: 5
Last seen: 3 years, 7 months
AMANITA MUSCARIA FAQ * 4
    #25702199 - 12/25/18 11:10 PM (3 years, 9 months ago)

Hello kind folks,

Seeing that it's Christmas day, and seeing as I've been meaning to move over my FAQ to this forum for a long time, I figure now is just as good a time as any to post some information about Amanita Muscaria.

Yes, it's me, the same Jillsa from other there. And I admit, it's been so long since I came to the mothership to post, I hope you will forgive me for forgetting how to do something as simple as upload an image. But please, rate this file if you liked it, and feel free to pass it on.
The more awareness we can bring to Amanita muscaria, the more, I feel we can reduce harm, and there is much to be benefitted.

I'll certainly be hoping to update this information as new information comes in - it turns out, that the active chemical constituent that Amanita is known for - Muscimol - is a very well beloved chemical in lab environments, due to its high binding affinity at the GABAA region.
Thus, it has over 377,000 entries on google. Many of them are peer reviewed lab reports, hosted at pubmed, science exchange, and more.

Without further ado, I must launch into any informative document with a precursor of warning. Muscimol is processed from Ibotenic acid, which is a known neurotoxin. Ibotenic acid has caused Olney's lesions in vivo animal experiments. (WO Guldin - ‎1982, P Winn - ‎1984 ). Olney's lesions have not been demostrated to occur in human beings with Ibotenic acid that I am aware of. However, the evidence is strong that this COULD occur. We are not seeking "Patient Zero," so please do not attempt to consume Amanita muscaria without proper preparation.

Regarding Dr Austin's patent for Muscimol preparation here (which I can summarize more simply below.) I've spoken to Dr Austin a few times in passing, and he is a lovely human. He has thought this through very well. In his citations for lab reports (  http://www.freepatentsonline.com/8784835.html ; 3, 4, ) there is a piece of critical information.

SIMPLY DRYING AMANITA MUSCARIA IS NOT ENOUGH TO DECARBOXYLATE (convert) IBOTENIC ACID INTO MUSCIMOL. (the citations specifically say that lab analysis showed only a 30% conversion.)

What do, fam?

This is where the rest of Dr Austin's patent comes in handy.

My peers and I have summarized an easier method, as follows. (we have not confirmed conversion rate by labs - but that will be forthcoming this spring.)

1) Properly identify your muscaroids. If you haven't presented a proper specimen for ID, which includes the bulb (volva), it is very likely you can not be sure that it is muscaroid, unless you are sure about the expert helping you with ID. Fortunately, amatoxic varieties are easy to avoid.

2) The mushrooms are first dehydrated. Chop them up for heaven's sake, and don't use the oven to dry them. They are very high moisture.

3) Into the oven at 80 celcius for 30 minutes.

4) Powder and place in ethanol (40%-50%) for two weeks. Muscimol is partially soluble in ethanol, more so in water.

5) Partially strain mushroom material. Steep mushroom material in hot water, then reduce. (you are responsible for the math!)

6) Tincture and concentrate are combined at a ratio to maintain 25% ABV for preservation. And also , storage in the refridgerator is recommended.

7) The product is roughly strained to allow some solids to remain. They tend to add to the taste (which ranges from umami to fruity, including some peers who said that Polish varieties tasted like fresh mangos...)

8)Both STEM and CAP should be used in the process, because the stem contains N-Acetyl-Cysteine, (NAC), which oddly enough is a very popular nootropic - helps to regulate glutathione, helps the body to process muscimol, helps regulate glutumate and gaba. (Notice ALPHA-GPC and CHOLINE here as well.) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4249817/

This is very interesting considering that muscimol exhibits lingand binding at nACHR7) https://www.researchgate.net/publication/282802731_GABAA_agonist_muscimol_ameliorates_learningmemory_deficits_in_streptozocin-induced_Alzheimer's_disease_non-transgenic_rat_model

9) My preferred ratio of tincture: 100 mg to 1 ml.

3 grams of dried material can be utilized to make a tincture with 30 ml. This tincture may last you an entire winter , with these threshold effects. Need I mention it also raises body temperature slightly, so if you're trying to sleep, and it's cold, you will be nice and toasty warm if you get up and take some on your tongue. (Or underneath is ok!)

10) Threshold effects at microdosing Amanita Muscaria tincture (9)
Increased rem and non rem sleep
https://www.ncbi.nlm.nih.gov/pubmed/8797193
Anxiolysis:
https://www.ncbi.nlm.nih.gov/pubmed/24473816
Tinnitus relief
https://www.ncbi.nlm.nih.gov/pubmed/10962189
Lyme disease abatement:
http://en.psilosophy.info/fly_agaric_tincture.html

and more.

Muscimol has some similarities to Varencycline (Chantix), so it's a good thing for people to try who are quitting tobacco (works on nicotinic acetycholine receptors)
In addition to its GABAA qualities, Muscimol has helped some peers to give up alcohol abuse.

In our opinion (the peer muscimol group and myself,) Muscimol has its best qualities in terms of visionary effect when one has actually fallen asleep after the dose. It is here where lucid dreams unfold.

Do not expect seratonergic effects from this fungus. Do expect that this fungus has abilities of its own.

If I began this article by telling you, that there is a fungus, that could increase the length of your sleep, the quality of your sleep, your ability to dream, and a host of other health benefits (as evidenced by the longevity of certain Nomadic cultures,) What then, pray tell, would you say to me?

Thank you for reading.
-JILLSA

The original file (which may have omissions here) https://www.facebook.com/notes/the-shroomery/amanita-muscaria-faq/1595105403931550/

Be a bit slow for a bit as I catch up with various stuff but happy to answer any questions.

A common question is about the "reactant" in Dr Austin's file. We've discussed it in several communities and we feel pretty sure that it's citric acid (to  bring PH down to 4.5-5.)


Edited by Jillsa Goodwitch (12/25/18 11:53 PM)


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OfflineLucisM
Nutritional Yeast

Registered: 03/29/15
Posts: 14,706
Last seen: 3 months, 20 days
Re: AMANITA MUSCARIA FAQ (moved) [Re: Jillsa Goodwitch]
    #25702227 - 12/25/18 11:39 PM (3 years, 9 months ago)

This thread was moved from The Pub.

Reason:
better fit


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OfflineJillsa Goodwitch
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Registered: 10/23/18
Posts: 5
Last seen: 3 years, 7 months
Re: AMANITA MUSCARIA FAQ (moved) [Re: Lucis]
    #25702246 - 12/25/18 11:54 PM (3 years, 9 months ago)

(dd)

thanks!


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Offlinemelanoxylon
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Registered: 03/15/18
Posts: 46
Last seen: 2 years, 10 months
Re: AMANITA MUSCARIA FAQ [Re: Jillsa Goodwitch]
    #25702777 - 12/26/18 06:23 AM (3 years, 9 months ago)

Thanks for the thread. I'll have to try making some tincture, but what's the purpose of leaving solids in it? Thoughts on this amanita and fruit juice concoction? I made some without going through all the sterilizing boiling but haven't drank any yet. Might be toxic? It grew some fleece at least.


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OfflineJillsa Goodwitch
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Registered: 10/23/18
Posts: 5
Last seen: 3 years, 7 months
Re: AMANITA MUSCARIA FAQ [Re: melanoxylon]
    #25704078 - 12/27/18 12:13 AM (3 years, 9 months ago)

Thanks for sharing the video.
I'm not sure how I feel about fleece. It's never been proven through DNA analysis, nor chemical GCMS, to be successful at creating muscimol, IIRC.

If you have any evidence to the contrary on that though, genuinely love to see it!

Some confusion has arisen as to whether the generated chemical would be Ibotenic acid. There is a fermentation process in Dr Austin's patent though, so if the fleece is a legitimate path to Muscimol (and not Mucor contaminant, as some of us suspect, it may be adequately decarboxylating.

As far as the other question with mushroom material, and leaving that in the tincture. We found that it added to the taste. Most of us in a specific study group decided to leave it in. It does not affect potency either way :smile: It's all about personal preference, in this case.


Edited by Jillsa Goodwitch (12/27/18 12:20 AM)


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Offlinemelanoxylon
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Registered: 03/15/18
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Last seen: 2 years, 10 months
Re: AMANITA MUSCARIA FAQ [Re: Jillsa Goodwitch]
    #25706499 - 12/28/18 03:53 AM (3 years, 9 months ago)

If ibotenic acid is being generated, might heating the final product decarb it? I'm no chemist obviously.

On another note, a few months ago, before I'd heard of amanita fruit juice, I combined amanita with acid and weed for the first time and got a long narrative about using homegrown apples to make amanita apple juice/cider, even including visuals of setting up a lemonaid stand on my front lawn to sell it : ). It's been a fun relationship.


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OfflineJillsa Goodwitch
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Registered: 10/23/18
Posts: 5
Last seen: 3 years, 7 months
Re: AMANITA MUSCARIA FAQ [Re: melanoxylon]
    #25718036 - 01/02/19 01:32 PM (3 years, 9 months ago)

"If ibotenic acid is being generated, might heating the final product decarb it? I'm no chemist obviously."

Yes. I'm speaking in reference to Teeter tek. AFAIK, no one has HEATED the resulting wine/juice...after the process is complete...

So in that instance, I was confused if the so called culture is generating IBO, or if that somehow in an aerobic process decarbs to MUS. But I will be investigating that this year, as well as the DNA research (Possibly with Alan R. he helped me to put my initial find on the map.) and so, I will certainly update here if I find out more information :smile:

Cute story on the "Lemonade stand." I do believe Amanita wants to be shared with the right audience, which is why I'm here :smile:

I admit, I have quite a lot of documents to look through on the mothership , to find out what other people's conjecture has been, and perhaps some facts that I have missed, but I am looking forward to it. Any questions, comments or concerns, please feel free to ask here.

-J


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Offlinethe reptile
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Registered: 07/02/18
Posts: 30
Last seen: 8 months, 3 days
Re: AMANITA MUSCARIA FAQ [Re: Jillsa Goodwitch]
    #26239997 - 10/09/19 08:18 PM (2 years, 11 months ago)

I have a few questions...
At how much degree do I reduce the material in hot water for how long or is that even important?
Is the recommended dilution (100mg for 1ml) the same if I do it without the stems (bec. I through threw them away before I red this thread)?
Last question... have you already done a lap analysis how much content ibotic acid is in the tincture according to this process?
Is the decarboxylation more effective if i use citric acid in part 5 and heat it up to 80°C?


Edited by the reptile (10/10/19 02:12 PM)


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Offlinemerkuryal
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I'm a teapot User Gallery
Registered: 09/13/16
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Re: AMANITA MUSCARIA FAQ [Re: Jillsa Goodwitch]
    #27007810 - 10/28/20 04:59 PM (1 year, 11 months ago)

Hello Jillsa
(I came here from a recent link in that other social media website)

Thank you for gathering and putting up this good information.

I have some questions regarding the advantage of an extract vs just eating the dried, baked and pulverized mush. Is there something we want to explicitly leave out? The way I understand it is maybe:
1. Shelf life (although we know that dried, baked and properly stored mush lasts a long time)
2. Ease of dosing (some drops or a spoonful of liquid as opposed to gel caps or smoothie?)
3. Even out the magic, as presumably different specimens will have differing concentrations of magic (but pulverizing and mixing the whole haul would achieve the same)

In short, caping, and eating sound to me an easier route than extracting, especially when we’re talking about such small quantities as micro dosing.

Btw, how much (maybe in a range) of dried mush matter do you consider a microdose?

The link to the patent is unfortunately dead

Thanks again!


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OfflineHeffer
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Registered: 12/18/21
Posts: 1
Last seen: 9 months, 13 days
Re: AMANITA MUSCARIA FAQ [Re: Jillsa Goodwitch]
    #27586295 - 12/18/21 11:38 PM (9 months, 13 days ago)

": Tincture and concentrate are combined at a ratio to maintain 25% ABV for preservation" What is ABV?
Also what does this ratio mean: My preferred ratio of tincture: 100 mg to 1 ml.
Thank you, have been using muscimol cream for almost a year for severe sciatica, game changer!


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OfflineNorthernerM
FSM acolyte
 User Gallery

Registered: 07/29/12
Posts: 11,352
Loc: FNQ
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Re: AMANITA MUSCARIA FAQ [Re: Heffer]
    #27586369 - 12/19/21 12:58 AM (9 months, 13 days ago)

ABV = alcohol by volume


--------------------
The nearest we ever come to knowing truth is when we are witness to paradox.


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Offlinetosatori
and beyond
Male

Registered: 01/20/07
Posts: 466
Last seen: 9 days, 23 hours
Re: AMANITA MUSCARIA FAQ [Re: Northerner]
    #27588737 - 12/21/21 04:45 AM (9 months, 11 days ago)

Good info. Merry Christmas


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