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OfflineJDoom413
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Registered: 11/29/18
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Last seen: 1 year, 9 months
Jars noc'd up. Feeling less than hopeful :(
    #25680431 - 12/15/18 10:14 AM (2 years, 2 months ago)

Hello my dudes , I just shot up my jars , my 1st try. Idk how it's gonna come out.

First of all I think the syringe was messed up. I took the sticker off of it so I couldn't see all the CC numbers and as I took it off solution started to drip out of the syringe , very slowly , but I could tell it was making my gloves wet.

2nd problem I could have had was that after each hole there was some substrate on the needle , I ignored this per jar and did all 4 holes. Flame sterilized after each jar though.

3rd issue , the needle was getting black from using a lighter to flame it. Idk if this is an issue , I'll just assume it is. I did get the needle glowing before each jar.

4th , my house is cold ! Like mid 50s a lot. I set my jars on my Mediacom cable box to keep them somewhat warmer. I made a little tent out of foil to put over them to kind of trap the warmer air in , around the jars. Air exchange can easily happen inside of it , the sides are mostly open to the air.

And now I have a question. I smoke a a cigarette or 2 a day and I also smoke 2 or 3 joints a day. Is it ok to smoke in the same room as the jars? Will they contaminate from the cig and ganja particles falling on them possibly?

Thank you !


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OfflineMike O.Rrhizal
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: JDoom413]
    #25680450 - 12/15/18 10:30 AM (2 years, 2 months ago)

Do you have a SAB for inoculation? They help a lot, cut my contamination by 70%ish.

1st ive had problems with needles but as long as your doing it in a sanitised SAB it will hopefully be ok. Its better that the needle was leaking than taking in unsterilised air.

2nd not sure about this one because my holes are quite small so vern docent come back out. Again hopefully will be ok because the dry verm layer should hopefully stop contams reaching you BRF.

3rd the needles go black its ok :thumbup:

4th your temps might be a problem 60+ is better.

5th don't worry about smoking there's way more contams flying around everywhere. I make while looking at my jars.

:bigblunt:


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OfflineMike O.Rrhizal
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: Mike O.Rrhizal]
    #25680455 - 12/15/18 10:32 AM (2 years, 2 months ago)

Forgot to say Good luck!


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OfflineJDoom413
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Registered: 11/29/18
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: Mike O.Rrhizal]
    #25680491 - 12/15/18 10:57 AM (2 years, 2 months ago)

Quote:

Mike O.Rrhizal said:
Forgot to say Good luck!





Thanks man. So maybe things will be ok. I was pretty sterile in my method. I didn't use the ghettos SAB I made. I sprayed bleach and 91% alcohol mix into the air. I used our 1/2 bathroom. The smallest room in the house. I hit all surfaces with the same mix. I let it sit on the surfaces for 20 min before I wiped it down. I made sure the exaust fan and heating vent were closed , so no moving air , except for my sterilized body. I wore a bandanna around my face so no breathing into the open room. I also wrapped one around my head to contain my hair.

I'm most worried about the leaky syringe and the spore ball didn't break up too well either.


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OfflineMadValley
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: JDoom413]
    #25680614 - 12/15/18 12:42 PM (2 years, 2 months ago)

I didn't have a leaky syringe but I couldn't get the spore ball to break up too well either. I shot through the micropore tape over the holes. The GTs are doing great almost 100% after two weeks. The PEs, no so much, but they're reported as being slower anyway so I'm not flipping out over them. Yet.

I think the thing that's easy to forget is just how many gazillion spores are in a syringe so even if a clump remains there are still way more than you need for a successful colonization just floating around.


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OfflineWillymycosnemesis
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: MadValley]
    #25680729 - 12/15/18 01:51 PM (2 years, 2 months ago)

If you haven't seen these, check them out to increase odds of success. 
https://www.shroomery.org/forums/showflat.php/Number/24144021


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OfflineSFS96S
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: JDoom413]
    #25681053 - 12/15/18 04:12 PM (2 years, 2 months ago)

You will probably have success but definitely check out the link Willy posted. Good luck to you:thumbup:


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Invisiblerainout
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: SFS96]
    #25681585 - 12/15/18 08:18 PM (2 years, 2 months ago)

1. WTF Why is your syringe leaking from the sticker? That cant be a normal syringe or a good one, maybe its leaking from the back?

2. That is how to do it, if the substrate was already contaminated on the jar putting it on another hole wouldn't make much of a difference anyway.

3. Not a problem at all, after making the syringe red from the hot its normal to make some soot.

4. Yeah 50F its not going to make mycelium, like at all, and even if it make some it will be so slow that it will take ages. If you dont make it warmer (at least mid 60's), with a pool heater or a heating pad, maybe growing will be impossible, consider getting one or another.

5. If you dont smoke directly to your incubator or the place where you store your jars you should be fine.


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OfflineJDoom413
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: rainout]
    #25681908 - 12/15/18 11:51 PM (2 years, 2 months ago)

Thanks guys. I feel a bit more positive now as the day has progressed. I was really sterile imo. I was really paranoid this morning when I did it , it created a really negative vibe for a couple hours.

I only did 3 jars anyway. I still have about 7 CCs left , a bit less. But that syringe is sketchy , it most definitely has a crack , I can see it , and the bag it came in that I put it back in has wetness from it leaking. It's right where the sticker was , covering the last 2 CCs ( 9 & 10 ).

I stopped being cheap for a while and set my thermostat to 62. The digital thermometer I have next to the jars says 67°. So that should be cool I hope. I put them on my Xbox one , it must emit a few degrees in "always on " mode.


Should I toss the syringe? I PCd a single jar , I was gonna blast the rest of the solution directly down the center of the jar and just say fuck it , it's more than likely compromised.


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OfflineIgnorantOne
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: JDoom413]
    #25681957 - 12/16/18 12:36 AM (2 years, 2 months ago)

1. The cracked syringe is no Bueno. I would be in contact with whatever vendor you got it from if I were you.

2. I would not worry about the sub, flaming the needle to red-hot takes care of it.

3. It happens. You did nothing wrong here.

4. Increase heat to at least 65.

5. I do the same, does not seem to affect the jars.

It would not hurt to finish the syringe off and toss it. Bandana works well for hair (again, I do the same) but you should invest in medical face masks. I think I spent under $5 for 40+ from Wally World.

As long as you followed the proper sterile procedure to the best of your ability I am confident you will see some success.


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Ignorance is bliss when tis folly to be wise.



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Edited by IgnorantOne (12/16/18 12:40 AM)


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InvisibleHarry S. Shrooman
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Registered: 11/19/18
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: JDoom413]
    #25681989 - 12/16/18 01:12 AM (2 years, 2 months ago)

Make a TiT incubator it’s super cheap and effective for your situation if you don’t wanna run a heater constantly


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InvisibleNEO15514
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Registered: 12/16/18
Posts: 57
Re: Jars noc'd up. Feeling less than hopeful :( [Re: Harry S. Shrooman]
    #25682009 - 12/16/18 01:33 AM (2 years, 2 months ago)

A Xbox gets pretty hot staying on all the time and being to warm can make your jars a breeding ground for contam.


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OfflineWillymycosnemesis
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: NEO15514]
    #25682826 - 12/16/18 01:10 PM (2 years, 2 months ago)

Incubators are neither necessary or advised.


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InvisibleHarry S. Shrooman
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: Willymycosnemesis]
    #25682874 - 12/16/18 01:31 PM (2 years, 2 months ago)

So you’re telling me he has better chances at temps around 50? There’s nothing wrong with using an incubator in his situation


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InvisibleNEO15514
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: Harry S. Shrooman]
    #25682938 - 12/16/18 02:01 PM (2 years, 2 months ago)

Quote:

Harry S. Shrooman said:
So you’re telling me he has better chances at temps around 50? There’s nothing wrong with using an incubator in his situation



but it's not a regulated heat source that stays at a certain degrees so yeah I wouldn't advise it


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OfflineIgnorantOne
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: NEO15514]
    #25683236 - 12/16/18 04:36 PM (2 years, 2 months ago)

Quote:

NEO15514 said:
Quote:

Harry S. Shrooman said:
So you’re telling me he has better chances at temps around 50? There’s nothing wrong with using an incubator in his situation



but it's not a regulated heat source that stays at a certain degrees so yeah I wouldn't advise it




I have a similar problem, my house is cold as fuck. What I have done is just place the jars up high in the room I spend time in. Heat rises so the temp up near the ceiling is warmer than on the floor. Temp still fluctuates up there but the average temp is higher than that at waist/shoulder level.

OP maybe temp up on the highest shelf you have in your main hangout room and see if that works better.


--------------------
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InvisibleHarry S. Shrooman
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: NEO15514]
    #25683315 - 12/16/18 05:23 PM (2 years, 2 months ago)

What? Yes it does stay at a certain degree I use a TiT bc I like my shit cold but am impatient so I have always incubated my jars at 74 degrees for quicker results. Never had any problems.


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OfflineMadValley
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: IgnorantOne]
    #25683317 - 12/16/18 05:24 PM (2 years, 2 months ago)

Something to think about for heat is one of these little controls.



They will switch on a control circuit for a high or low set point. This one's wired to cool right now (I used it to keep my cheese fridge in temp when I was making cheese) but I'm going to switch it over to control a heater on my FC (I'm going to use Mateah's humidity chamber tech).



The control is really easy to wire and it's got like an 8' temp probe.



So I'll just put it in the hydro-substrate and set it to 72F. That way the cakes will always be at or below 72F and I won't have to heat the whole room.


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OfflineWillymycosnemesis
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: MadValley]
    #25683408 - 12/16/18 06:10 PM (2 years, 2 months ago)

You should always heat the room not the jars or tubs.


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OfflineMike O.Rrhizal
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Re: Jars noc'd up. Feeling less than hopeful :( [Re: Willymycosnemesis]
    #25685010 - 12/17/18 12:49 PM (2 years, 2 months ago)

:whathesaid:
don't put them on your xbox


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Mushrooms, Mycology and Psychedelics >> Getting Started

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