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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,615
Loc: Oregon, USA
Last seen: 9 hours, 46 minutes
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Re: Working with filter patch bags, some tips [Re: daba]
#1577171 - 05/25/03 09:40 PM (21 years, 6 months ago) |
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Yeah, they are high. I guess if they didn't have that selfhealing port they would be cheaper. Might ask if they have any without the port.
-------------------- Research funded by the patrons of
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Anonymous
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Re: Working with filter patch bags, some tips [Re: Workman]
#1577213 - 05/25/03 09:53 PM (21 years, 6 months ago) |
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Indeed. If you are ready to handle 1000's of bags, you can get them with free shipping and very low prices at www.unicornbags.com. I believe that is where Mycobag gets their bags from, as well as SporeWorks.
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Working with filter patch bags, some tips [Re: Una] 1
#1577703 - 05/26/03 02:04 AM (21 years, 6 months ago) |
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I hope this image makes things clearer.
Note that the water level is BELOW the rack.
-------------------- www.911blogger.com
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Working with filter patch bags, some tips [Re: Workman]
#1577840 - 05/26/03 05:24 AM (21 years, 6 months ago) |
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Quote:
There are new bags at MycoBag that can be sealed before sterilizing without bursting.
I have done that several times with the previous bags...bent the second rack all to hell but the bags held together....
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Working with filter patch bags, some tips [Re: Baby_Hitler]
#1577846 - 05/26/03 05:34 AM (21 years, 6 months ago) |
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Quote:
How full was the pressure cooker? How tight was the bag? I was going to tie the bag tight so that there was zero room for them to expand.
in a cloth it'll work....I tried it with tape a time or two and had a small blowout and the other 2 bags ripped when I removed the tape....but dropping them into a sturdy pillow case and taping around that has worked quite well....lots of tape...dont use express or priority mail tape....it bonds together
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Working with filter patch bags, some tips [Re: daba]
#1577849 - 05/26/03 05:38 AM (21 years, 6 months ago) |
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Quote:
Indeed.
If you are ready to handle 1000's of bags, you can get them with free shipping and very low prices at www.unicornbags.com.
I believe that is where Mycobag gets their bags from, as well as SporeWorks.
nope...I tried...they refered me to spore works and now to mycobag....they are aparently the US distributor for unicorn....and believe me....I'm not on a shoe string budget....10,000 bag order didnt sway them...maybe under a contract....who knows.... I will try again or contract it to another company....there are 2 more that produce spawn bags that I'm aware of...just have never ordered from them....
Edited by Prisoner#1 (05/26/03 05:42 AM)
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Baby_Hitler
Errorist
Registered: 03/06/02
Posts: 27,879
Loc: To the limit!
Last seen: 8 hours, 50 minutes
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Re: Working with filter patch bags, some tips [Re: Prisoner#1]
#1577968 - 05/26/03 08:53 AM (21 years, 6 months ago) |
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http://www.mycobags.com and http://www.mycobag.com are two different companies. Mycobag is the sporeworks spinoff.
Quote:
but dropping them into a sturdy pillow case and taping around that has worked quite well....lots of tape...dont use express or priority mail tape....it bonds together
I was just going to tie a knot in it.
I'm planning on making a high temperature silicone injection site too. Pre-puncturing the injection site may help relieve some pressure.
-------------------- Not emotionally invested in the well-being of Trump supporters.
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: Working with filter patch bags, some tips [Re: Baby_Hitler]
#1579820 - 05/26/03 09:55 PM (21 years, 6 months ago) |
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3 out of 3 times I had coontacted unicorn bags I was given a referal to spore works, and the last was to mycobag.....they also provide to a few other but how that chain is linked is beyond me....but I started about a year and a half ago calling in reference to the products at unicorn....maybe they just dont wish to sell to me.
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Working with filter patch bags, some tips [Re: Prisoner#1]
#1597799 - 06/01/03 06:58 AM (21 years, 6 months ago) |
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Here's a tip for people in Europe. "De Spore" is the european distributor of the Unicorn bags. This is the company from Peter Oei (author of the book "mushroom cultivation").
The cost (if i remember correctly) is 22 euro cents per bag.
Their homepage can be found HERE
English page about the spawnbags HERE
-------------------- www.911blogger.com
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micro
bunbun has a gungun
Registered: 05/09/03
Posts: 7,532
Loc: Brick City
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Re: Working with filter patch bags, some tips [Re: Una]
#1606308 - 06/03/03 09:22 PM (21 years, 6 months ago) |
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I just had an idea about making spore prints; never tried it, though. Why not just fruit 1 bag in vitro and take a cap or 3 or 4 off (in vitro, using the outside of the bag,) and flip the bag on its side, take the print on the bag and then fold that part and heat seal it? This should be completely sterile, and you could always just inject a little sterile DI water into your spore pouch, mix + pull up to make a seringe.
Just an idea....
-- Micro
-------------------- Any research paper or book for free
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Baby_Hitler
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Re: Working with filter patch bags, some tips [Re: micro]
#1607360 - 06/04/03 08:12 AM (21 years, 6 months ago) |
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It would be difficult to manipulate the caps through the bag, but you might could just turn it on it's side, and let the spores fall on the side of the bag. You wouldn't get a "print" exactly, but you would get plenty o spores.
-------------------- Not emotionally invested in the well-being of Trump supporters.
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sci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
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Re: Working with filter patch bags, some tips [Re: Baby_Hitler]
#2567506 - 04/16/04 11:25 AM (20 years, 7 months ago) |
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Not to bring up an old thread but this is informative and I was wondering, couldn't you just take the sealed bag and pasteurize it? For grain/superspawn innoculations.
-------------------- Looking for:
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debianlinux
Myconerd - DBK
Registered: 12/09/02
Posts: 8,334
Loc: Over There
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Re: Working with filter patch bags, some tips [Re: sci33]
#2567634 - 04/16/04 12:10 PM (20 years, 7 months ago) |
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i'm confused, i think...
grains have to be sterilised, pasteurisation doesn't cut it with grains
i assume your talking about pasteurising straw in the bags to spawn and grow like a straw log?
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sci33
AmbitiousScientist
Registered: 11/11/03
Posts: 186
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Re: Working with filter patch bags, some tips [Re: debianlinux]
#2567640 - 04/16/04 12:14 PM (20 years, 7 months ago) |
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Yes, I mean mixing up the dung/straw mixture in a filter patch bag, pasteurizing it in a water bath of 170 degrees for an hour or so, allowing it to cool down in a flowhood, then innoculating it with colonized grain or superspawn innoculation - and then sealing the bag.
That just seems like the quickest method to colonize the substrate to me rather than a syringe innoculation on sterilized substrate.
-------------------- Looking for:
Ps. weilii, Ps. samuiensis, Ps. hispanica, Pan. cyanescens (any strain I don't have)
Have:
Ps. cubensis, Ps. tampanensis, Pan. cyanescens (Australian, Tai-KS, Hawaiian, Jamaican, Ban Lipa Noi)
PM for a trade.
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