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globejs
lollygagger
Registered: 08/18/07
Posts: 414
Last seen: 5 months, 19 days
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My microscope showed up! 1
#25668118 - 12/09/18 02:40 PM (5 years, 3 months ago) |
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My nikon alphaphot 2 scope showed up yesterday! The slides, cover slips, and immersion oil I ordered all showed up too so I got to play around right away.
I got a big bonus with the scope too, a phase contrast condenser! Looking up other nikon phase contrast condensers online is crazy. Someone is selling this exact one on eBay for $379
Now to find a reticle and calibration slide!
-------------------- NEWT TESTED, NEWT APPROVED.
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karode13
Tāne Mahuta
Registered: 05/19/05
Posts: 15,290
Loc: LV-426
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Re: My microscope showed up! [Re: globejs]
#25668341 - 12/09/18 04:42 PM (5 years, 3 months ago) |
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Looks nice.
If you need a tutorial there's one floating around somewhere. Let me know.
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Doc9151
Mycologist
Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 8 months
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Re: My microscope showed up! [Re: globejs]
#25668358 - 12/09/18 04:51 PM (5 years, 3 months ago) |
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Looks like someone will be busy playing with their new toy, I hope it brings you lots of enjoyment.
I love having a microscope, there is so much to look at, whether it's mushrooms, bugs or biological fluids.
It can be used without a power source by using a mirror or flashlight. If the specimens are really dense, such as a rock, you can use a flashlight to illuminate the surface and use it like a stereoscope, just be careful to not touch the objective to whatever you're looking at. I'm excited for you and look forward to seeing some micrographs through the eye piece.
-------------------- Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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globejs
lollygagger
Registered: 08/18/07
Posts: 414
Last seen: 5 months, 19 days
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Re: My microscope showed up! [Re: Doc9151]
#25669587 - 12/10/18 09:07 AM (5 years, 3 months ago) |
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A tutorial would be very helpful! I've already been checking out my hair, my pets hairs, and some spores and a "tooth" from a hedgehog mushroom I found. The phase contrast setting definitely makes for better viewing. Objective progression on a the piece of hedgehog "tooth". Saw my first basidium!
-------------------- NEWT TESTED, NEWT APPROVED.
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karode13
Tāne Mahuta
Registered: 05/19/05
Posts: 15,290
Loc: LV-426
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Re: My microscope showed up! [Re: globejs]
#25670646 - 12/10/18 05:01 PM (5 years, 3 months ago) |
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Good start!
I'm having trouble uploading to here. If you have facebook then you might be able to use this link>>>Microscopy tutorial
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Anglerfish
hearing things
Registered: 09/08/10
Posts: 18,741
Loc: Norvegr
Last seen: 7 hours, 8 minutes
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Re: My microscope showed up! [Re: karode13]
#25671302 - 12/11/18 12:39 AM (5 years, 3 months ago) |
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Quote:
karode13 said: you might be able to use this link>>>Microscopy tutorial
This link appears to be dead, and has been so for years I believe. Too bad, it was a good tutorial.
-------------------- ★★★★★
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karode13
Tāne Mahuta
Registered: 05/19/05
Posts: 15,290
Loc: LV-426
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Re: My microscope showed up! [Re: Anglerfish]
#25671602 - 12/11/18 06:19 AM (5 years, 3 months ago) |
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I thought that might happen as it's uploaded in a private group. I'll put it on the list of things to do as I'm about to sleep.
We should have it uploaded here. Alan has it, maybe he is able to post it.
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Anglerfish
hearing things
Registered: 09/08/10
Posts: 18,741
Loc: Norvegr
Last seen: 7 hours, 8 minutes
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Re: My microscope showed up! [Re: karode13]
#25671678 - 12/11/18 07:12 AM (5 years, 3 months ago) |
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Quote:
karode13 said: I thought that might happen as it's uploaded in a private group. I'll put it on the list of things to do as I'm about to sleep.
We should have it uploaded here. Alan has it, maybe he is able to post it.
I don't think the guide is available anywhere online, and the user who originally posted it hasn't been around for over three years.
This one should be helpful enough, though:
https://www.mushroomexpert.com/microscope.html
-------------------- ★★★★★
Edited by Anglerfish (12/11/18 07:19 AM)
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karode13
Tāne Mahuta
Registered: 05/19/05
Posts: 15,290
Loc: LV-426
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Re: My microscope showed up! [Re: Anglerfish]
#25672676 - 12/11/18 03:42 PM (5 years, 3 months ago) |
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We have a pdf version ripped from the web. I'll get onto it as I'm now awake.
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globejs
lollygagger
Registered: 08/18/07
Posts: 414
Last seen: 5 months, 19 days
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Re: My microscope showed up! [Re: karode13]
#25673131 - 12/11/18 07:20 PM (5 years, 3 months ago) |
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Is there any way to differentiate different types of mycellium under a microscope? I'm hoping it's agarikon, but it could be a couple different things based on what I've collected cultures from. It's looking like this after about 2 weeks. Soft leading edges, near the inoculation point there are tiny, slightly whiter dots of mycellium evenly spaced. Maybe I should take a piece out and do a smell/ taste test. Not sure how much different mycellium resembles the scent and flavor of its fruiting body, but I know cubes are nearly identical. I know what agarikon tastes and smells like. One of the cleanest, medicinal, potent fungi scents I've experienced.
-------------------- NEWT TESTED, NEWT APPROVED.
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globejs
lollygagger
Registered: 08/18/07
Posts: 414
Last seen: 5 months, 19 days
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Re: My microscope showed up! [Re: globejs]
#25673183 - 12/11/18 07:46 PM (5 years, 3 months ago) |
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Also is there any discernable characteristics of cubensis spores and psilocybe cyanescens that could be used to distinguish the two from eachother? I have 30 prints. Putting them all together in a move was not my finest moment nor was forgetting to use any indicating marking or label. Possibly the overall size of the print or density could help me separate some of them apart
-------------------- NEWT TESTED, NEWT APPROVED.
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Doc9151
Mycologist
Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 8 months
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Re: My microscope showed up! [Re: globejs]
#25675360 - 12/12/18 07:04 PM (5 years, 3 months ago) |
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You have to look at more than just the spores when using microtaxonomy to help you identify your mushrooms, it is a combination of macro and microscopic characteristics. Type, shape and size of basidia, cystidia, as well as the type of cystidia that is or isn't present, their color, fluid contents if any and its color.
This is just a small piece of the puzzle, start with learning how to identify the taxonomic structures, making quality slides and taking measurements, then move on to the next level.
Take your time, it's easy to become overwhelmed with information, look at as many species as possible and read the descriptions of each one, taking the time to look at it under the microscope and finding each structure in the description. This can be a very rewarding hobby, to me anything learned is a valuable asset and mycology will definitely provide a lifetime of learning.
Wishing you the best,
Doc
-------------------- Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
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globejs
lollygagger
Registered: 08/18/07
Posts: 414
Last seen: 5 months, 19 days
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Re: My microscope showed up! [Re: Doc9151]
#25675818 - 12/12/18 11:08 PM (5 years, 3 months ago) |
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Oh Yes I plan to use this as a tool for identifying specimens I find out foraging, but the situations I have before me are that I have an "unlabeled slant and 30 spore prints of 2 mushroom species.
I wasn't sure if the two species I mentioned in my earlier post had characteristics that were not shared between the two species at the spore level in order to tell them apart as growing out each print to produce fruits to identify would be quite a long and laborious process, but maybe that's all I can do
Thanks for your help!
-------------------- NEWT TESTED, NEWT APPROVED.
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