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Offlinerockyfungus
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Registered: 03/01/21
Posts: 1,062
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hindsight]
    #27612303 - 01/08/22 10:59 PM (2 years, 19 days ago)

cat shit and wood agar all day baby

Edit: plates
Slowly making my way through backlog


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InvisibleDoctor Mario
๐Ÿ„๐Ÿ„๐Ÿ„๐Ÿ„๐Ÿ„
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Re: AGAR ENVY! (Anything and All things agar!) [Re: rockyfungus] * 6
    #27612363 - 01/09/22 12:54 AM (2 years, 19 days ago)



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OfflineSpaceBaby
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Registered: 11/01/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doctor Mario] * 2
    #27613333 - 01/09/22 06:48 PM (2 years, 18 days ago)

APEU T5s this morning



Pulled that pigeon out of the fire!  :rockon:


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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby]
    #27613354 - 01/09/22 07:02 PM (2 years, 18 days ago)

Nice lookin plates, Space! I can't tell if my APEU germ plates are heavily tomentose mycelium or white mold or some sort :shrug: I guess i'll just keep transferring and hope for the best lol


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OfflineA.k.aM
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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 2
    #27613366 - 01/09/22 07:13 PM (2 years, 18 days ago)

Apeu has been extremely tomentose the couple times I had it on plates.


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LAGM2020


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27613489 - 01/09/22 09:21 PM (2 years, 18 days ago)

That's good to hear. Strangely enough my RW at T1 is looking like it's showing potential to go rhizo which would be a pleasant surprise


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I :heart: 5318008


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OfflineSpaceBaby
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Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #27613554 - 01/09/22 10:14 PM (2 years, 18 days ago)

Thanks. Yeah I almost lost my APE and APEU. Working out finally...

Hey unc, dunno, i keep transferring and noccing grains and some shit works out. I ain't the level of precise recipe science that some of you guys are, so my recipe varies and even my go to LME/P is different, at least a bit, everytime. In fact, this week it''ll be 20g agar, 6g LME, 1g NY, 1g P to each L of distilled water. Of course those are targets and sometimes I spazz the addition of the powder, lose some control. [neuropathy :shrug:] I'll see how that goes.

Would you guys run the same recipe less the agar for LC broth?


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:



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InvisibleInfinite Monkeys

Registered: 09/01/21
Posts: 276
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27614438 - 01/10/22 07:41 PM (2 years, 17 days ago)



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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Infinite Monkeys]
    #27614448 - 01/10/22 07:50 PM (2 years, 17 days ago)

I have some 4-year old swabs that don't work. Trying to get them to work. I PCed some distilled water, let it cool. Cut the tips off 2 swabs, dropped into 2 syringes. Sucked up .5 ml of the water into each one. Capped the syringes. Did all this in front of flow hood.

One syringe I am leaving as was, the other one I added a rubber band to give some compression.

Any ideas how many days I should leave them soaking before using? Thank you.


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-NEW? Start here.


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OfflineiceNock


Registered: 11/15/21
Posts: 335
Last seen: 5 months, 6 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27614728 - 01/11/22 05:37 AM (2 years, 17 days ago)

What's a good/reliable tek for modifying the traditional widemouth quart metal lids that'll only be colonized by agar transfers?


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Offlinecatcake1
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Registered: 10/03/21
Posts: 38
Last seen: 1 year, 1 month
Re: AGAR ENVY! (Anything and All things agar!) [Re: iceNock]
    #27614754 - 01/11/22 06:23 AM (2 years, 17 days ago)

what do you guys think is this clean growth?

this one looks contaminated with this white mold spots. i wonder if the mold is intertwined


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Invisiblemilkboy
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Registered: 05/01/21
Posts: 2,295
Re: AGAR ENVY! (Anything and All things agar!) [Re: catcake1]
    #27614795 - 01/11/22 07:23 AM (2 years, 17 days ago)

1st on looks ok to me, I think most people would do another transfer, definitely another transfer on the 2nd pic.

APEU swab plate and T1 both pinning


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Gummies by milkboy
Milkboys Public Display of Affection

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OfflineA.k.aM
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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy] * 4
    #27614824 - 01/11/22 07:58 AM (2 years, 17 days ago)

Perfect example of why you should take multiple pieces from a germ plate.




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LAGM2020


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OfflineShu
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27615016 - 01/11/22 10:56 AM (2 years, 16 days ago)

Quote:

nektar61 said:
I have some 4-year old swabs that don't work. Trying to get them to work. I PCed some distilled water, let it cool. Cut the tips off 2 swabs, dropped into 2 syringes. Sucked up .5 ml of the water into each one. Capped the syringes. Did all this in front of flow hood.

One syringe I am leaving as was, the other one I added a rubber band to give some compression.

Any ideas how many days I should leave them soaking before using? Thank you.





Zero days.


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shu]
    #27615329 - 01/11/22 03:49 PM (2 years, 16 days ago)

Quote:

Shu said:
Quote:

nektar61 said:
I have some 4-year old swabs that don't work. Trying to get them to work. I PCed some distilled water, let it cool. Cut the tips off 2 swabs, dropped into 2 syringes. Sucked up .5 ml of the water into each one. Capped the syringes. Did all this in front of flow hood.

One syringe I am leaving as was, the other one I added a rubber band to give some compression.

Any ideas how many days I should leave them soaking before using? Thank you.





Zero days.




Meaning it won't work, or meaning it takes just hours or minutes if it's going to work?


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Offlinem0n0id
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Registered: 10/08/21
Posts: 88
Last seen: 1 month, 17 days
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27615376 - 01/11/22 04:41 PM (2 years, 16 days ago)

Quote:

nektar61 said:
Quote:

Shu said:
Quote:

nektar61 said:
I have some 4-year old swabs that don't work. Trying to get them to work. I PCed some distilled water, let it cool. Cut the tips off 2 swabs, dropped into 2 syringes. Sucked up .5 ml of the water into each one. Capped the syringes. Did all this in front of flow hood.

One syringe I am leaving as was, the other one I added a rubber band to give some compression.

Any ideas how many days I should leave them soaking before using? Thank you.





Zero days.




Meaning it won't work, or meaning it takes just hours or minutes if it's going to work?





It's gonna be fine. I think they're saying you don't need to wait. But, given that they're kinda old it probably doesn't hurt, probably is actually beneficial to wait several days. I mean, spore syringes are common and those are good after months of soaking.


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OfflineA.k.aM
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Re: AGAR ENVY! (Anything and All things agar!) [Re: m0n0id] * 1
    #27615383 - 01/11/22 04:48 PM (2 years, 16 days ago)

Itโ€™s pretty common to be pressure hydrate old spores like that. Definitely wonโ€™t hurt.


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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #27615411 - 01/11/22 05:12 PM (2 years, 16 days ago)

Quote:

A.k.a said:
Itโ€™s pretty common to be pressure hydrate old spores like that. Definitely wonโ€™t hurt.





Thanks all. I'll give 'em 4 days total.

Here's a happy little tree of Orissa mycelium. I just transferred away from 4 oclock and 6 oclock.


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OfflineInthepit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 3
    #27616100 - 01/12/22 11:34 AM (2 years, 15 days ago)

I just wanna say my last batch of 24 agar plates had 0 contams, a personal best.
Perhaps it was washing my forearms and gloves.
That was added to wiping everything down with a wet bleach towel, put under the rack.

Of course it all adds up, I move slower, especially taking the cover off a plate.
I even pause after moving stuff around getting ready for the next plate.

I tried incense today and it's cool to see what air currents happen.

Incense is for temple!


Edited by Inthepit (01/12/22 11:34 AM)


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: Inthepit]
    #27616112 - 01/12/22 11:50 AM (2 years, 15 days ago)

Quote:

Inthepit said:
I just wanna say my last batch of 24 agar plates had 0 contams, a personal best.
Perhaps it was washing my forearms and gloves.
That was added to wiping everything down with a wet bleach towel, put under the rack.

Of course it all adds up, I move slower, especially taking the cover off a plate.
I even pause after moving stuff around getting ready for the next plate.

I tried incense today and it's cool to see what air currents happen.

Incense is for temple!




Nice! I keep wanting to do the incense test but that stuff gives me and my wife splitting headaches for hours if we're around it. I've recently started wiping my forearms with iso and sterilizing my tools as well and have seen notable improvements with all my transfers


--------------------



I :heart: 5318008


NOT a virgin!


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