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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,655
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon] 1
#28631954 - 01/23/24 10:00 AM (4 days, 22 hours ago) |
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Quote:
mycosispeon said: Need some advice from y'all experts. I took a T1 five days ago on this TAT dish, but now the mycelium on the germ plate looks better. Should I wait it out, or do I go back into the germ plate and take another T1 (not knowing whether the mold has sporulated or not)?

New plate looks pretty good, but I would definitely go back to germ plate and get 5 or 6 more transfers to new plates
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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] 1
#28632006 - 01/23/24 10:45 AM (4 days, 21 hours ago) |
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 Rhizo isn't everything, but damn that is pretty rhizo.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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tryptkaloids
Learner



Registered: 02/08/15
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ReverendMyc]
#28632253 - 01/23/24 03:52 PM (4 days, 16 hours ago) |
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Alright guys, finally time to do this. I have the lc ready, but no idea what container to use.
Any ideas? All I have is 1/4 pint jars, or syringes without caps.
Quote:
Nichrome said:
Quote:
tryptkaloids said:
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Nichrome said: What % of nutrition in your mix? Across the board it just looks like a minor media resource imbalance of some kind but honestly not too bad.
This was just a phenomenon I noticed pulling cultures from cold storage over time so I started employing the occurrence on purpose to help with my own goals.
These are just my crazy thoughts so take it as you will. Grain of salt and all that. 

These plates are 10g agar which I usually dial back a little bit And 9g potato flakes to 500ml
They came from lme, I don't remember the recipe but I usually run it at 7g for 500ml
Nothing else.
I'll definitely try an lc in cold storage. Should I let it grow out a little before the fridge or put it in there right after I make it?
It'll give me the time to wait for real plates to come in and work the other cultures on some no pours
Fully mature LC. Teaming with metabolites, and all the nutrition stored up in tissue is best.
No GE for cold storage. Try to leave as small of a bubble as possible so the oxygen gets used up and is not an inspirational factor for metabolic processes. leaving the bottled LC to sit at room temp for a couple days before the cold storage helps to use up the remaining oxygen. The goal is to slow metabolism to a near halt so they enter an induced meditative state. Light, heat, oxygen, and nutrition are the factors to limit, nutrition being limited in the form of already having been consumed. In a distractionless space they have the room for thought, self reflection, and self evaluation. They come out a bit changed and more mature.
Just going from LC to agar alone will often break a tricky culture. I'd also suggest plating some at the time of bottling. You may see enough of a break at that point to reach your goals without waiting for the culture in cold storage to figure it out. Out of the cold they break more but you don't always need to go that far. These types of methods require some creative thinking and good intuition on behalf of the cultivator but these types of methods also give rise to more opportunity for observation of behavior.
I wish I had better terminology but I don't so I use terms like "meditative state" because that is the most similar term I know of, that commonly I think others can relate to, to describe what I am trying to convey...
Please let me know how this goes for you so I can expand my understanding as well.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Changed


Registered: 05/07/23
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Loc: 38.864, -77.0573
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] 4
#28632450 - 01/23/24 06:33 PM (4 days, 13 hours ago) |
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My prettiest plate so far...
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CocaineBuffet
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon]
#28632675 - 01/23/24 09:46 PM (4 days, 10 hours ago) |
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Quote:
mycosispeon said: Need some advice from y'all experts. I took a T1 five days ago on this TAT dish, but now the mycelium on the germ plate looks better. Should I wait it out, or do I go back into the germ plate and take another T1 (not knowing whether the mold has sporulated or not)?

If you got the plates to spare you can take another T1 but I have a hunch if you took the transfer where the gap was on the first plate it will probably look pretty damn good when you let it grow out.
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 77
Loc: PNW
Last seen: 12 hours, 57 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: CocaineBuffet]
#28633052 - 01/24/24 09:00 AM (3 days, 22 hours ago) |
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Quote:
CocaineBuffet said:
Quote:
mycosispeon said: Need some advice from y'all experts. I took a T1 five days ago on this TAT dish, but now the mycelium on the germ plate looks better. Should I wait it out, or do I go back into the germ plate and take another T1 (not knowing whether the mold has sporulated or not)?

If you got the plates to spare you can take another T1 but I have a hunch if you took the transfer where the gap was on the first plate it will probably look pretty damn good when you let it grow out.
That is where I took the transfer from. And at the time, it was the only candidate sector. However, there was also that wispy mold in close proximity.
-------------------- LAGM 2.024 - I'm new so take everything I say with a grain of salt
Edited by mycosispeon (01/24/24 09:01 AM)
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arnold582

Registered: 10/30/23
Posts: 37
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mycosispeon]
#28633208 - 01/24/24 11:34 AM (3 days, 20 hours ago) |
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Is bacterial contamination on agar usually obvious? I've made around 25 plates so far and I've lost 1 to mold, every other plate has been fully taken over by white myc.
Does that mean that my technique is somewhat decent, or could I have just easily missed bacterial contamination hiding somewhere?
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,655
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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582] 2
#28633222 - 01/24/24 11:55 AM (3 days, 20 hours ago) |
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Quote:
arnold582 said: Is bacterial contamination on agar usually obvious? I've made around 25 plates so far and I've lost 1 to mold, every other plate has been fully taken over by white myc.
Does that mean that my technique is somewhat decent, or could I have just easily missed bacterial contamination hiding somewhere?
Yes, bacteria looks like snot. Usually hard to miss. IME, mold is the sneaky one.
Got agar pics?
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arnold582

Registered: 10/30/23
Posts: 37
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] 1
#28633337 - 01/24/24 01:34 PM (3 days, 18 hours ago) |
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That's good to know, thanks. Here are a few recent ones:
Transfer 3, I used this to inoculate spawn:

Transfer 1:

Clone from a relatively large fruit that was growing quickly inside an agar dish:
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,655
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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582] 1
#28633422 - 01/24/24 02:44 PM (3 days, 17 hours ago) |
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They don't look obviously contaminated. So I would say your tek is good. What happened when it went to grain/bulk?
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arnold582

Registered: 10/30/23
Posts: 37
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
#28633448 - 01/24/24 03:18 PM (3 days, 16 hours ago) |
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Quote:
LadysKnight said: They don't look obviously contaminated. So I would say your tek is good. What happened when it went to grain/bulk?
Nothing too bad happened, this was my first time working with grain spawn and I accidentally stalled out my jars with poor gas exchange, but it's fruiting now and I'm about to get my first flush 

The reason I asked is because I want to experiment with liquid inoculant for my next batch and I was pondering whether I might've missed something on my agar plates because I honestly expected to lose more of them as a beginner. You've given me enough confidence to at least give it a try, and if it doesn't pan out, at least I'll know that I have a problem to fix. Thank you!
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 1,655
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight] 1
#28633603 - 01/24/24 05:36 PM (3 days, 14 hours ago) |
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Right on, then you are winning. Your agar game is pretty good if you got this far. There are a couple suspicious areas, but see how it develops. How long did it take from spawn-to-bulk, til now? Are they getting light? Air exchange?
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arnold582

Registered: 10/30/23
Posts: 37
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LadysKnight]
#28633701 - 01/24/24 07:07 PM (3 days, 12 hours ago) |
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So, it's been 17 days so far, I spawned my jars on 7th (1:2) and the surface started getting covered with pins on 22nd.

I realized that many areas of this photo have a vague greenish hue but it's just the lighting and an old smartphone camera.
They get plenty of ambient light but I haven't increased FAE yet because the air here is very dry and I didn't want to risk drying out the substrate. They get some FAE through the lid that doesn't fit snugly, and I fan them twice a day. I'm waiting for the pinset to fill out before I flip the lid and really ramp up FAE, in around 2-3 days or so? Surface conditions seem fine too, there are still a few tiny beads of water on the surface from my last misting a few days ago. I'm very optimistic at this point
Edited by arnold582 (01/24/24 07:22 PM)
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582] 2
#28633716 - 01/24/24 07:23 PM (3 days, 12 hours ago) |
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Don’t fan, mist until it’s covered in tiny beads but not pooling, and then don’t touch it. There is no reason to fan your grow twice a day. Your surface is hella dry.
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arnold582

Registered: 10/30/23
Posts: 37
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
#28633725 - 01/24/24 07:30 PM (3 days, 12 hours ago) |
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Wait, really? There are still those tiny beads of water sitting on the substrate, though pins have slurped up a lot of it in the past day or so. Here's a better quality photo:
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rumfor69
Bodhicitta Cultivator



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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582] 1
#28633728 - 01/24/24 07:34 PM (3 days, 12 hours ago) |
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Either way, stop fanning it's only counter productive and does more harm than good
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Smellyhobbit
Actual Retard



Registered: 04/01/22
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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582] 1
#28633737 - 01/24/24 07:41 PM (3 days, 12 hours ago) |
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Quote:
arnold582 said: Wait, really? There are still those tiny beads of water sitting on the substrate, though pins have slurped up a lot of it in the past day or so. Here's a better quality photo:

Your pins aren’t drinking the water, you’re fanning it away. You want the whole thing to have thousands of little beads of water on it.
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arnold582

Registered: 10/30/23
Posts: 37
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] 2
#28633742 - 01/24/24 07:46 PM (3 days, 12 hours ago) |
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Whoops, I'll give them a mist and stop fanning then. Thank you both 
edit: Thoroughly misted
Edited by arnold582 (01/24/24 07:59 PM)
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Luminous7


Registered: 01/26/16
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Re: AGAR ENVY! (Anything and All things agar!) [Re: arnold582]
#28634227 - 01/25/24 09:35 AM (2 days, 22 hours ago) |
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Is this neaky mold comming forth?

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ReverendMyc

Registered: 03/29/19
Posts: 1,494
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Luminous7] 7
#28634371 - 01/25/24 11:12 AM (2 days, 20 hours ago) |
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Could be. I would get a transfer from 7 o'clock 1st picture and keep an eye on it.
x-poxt from plins thread. Because...
I love lions mane on agar.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
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