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OfflineShaperDreaming
Weirdo
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Registered: 10/30/18
Posts: 3,429
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #25992017 - 05/14/19 10:49 AM (4 years, 10 months ago)

Here's what's in the stuff I use:

Quote:

WATER, SUGAR, FD&C YELLOW #5, FD&C BLUE #1, PROPYLENE GLYCOL, FD&C RED #40, FD&C YELLOW #6, FOOD STARCH-MODIFIED (CORN), FD&C RED #3, CITRIC ACID, XANTHAN GUM, SODIUM BENZOATE (PRESERVATIVE), POTASSIUM SORBATE (PRESERVATIVE).




water then sugar then colors (basically)

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OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25992376 - 05/14/19 02:09 PM (4 years, 10 months ago)

@SD Myc doesn't only grow on the surface of agar.

I have Phoenix Oyster on 1.5/2%MEA. When I transfer, I see fluff growing from all sides of the wedge in a matter of hours? Seems to me to be growing inside the agar.

Also, the nutrition has impact on the type and speed of growth. I usually use 2% for both agar and LME. I've been experimenting with 1.5% LME and myc covers more ground. The general asumption in here seems to be that it is 'seeking' nutrient, so grows faster. Definitely less 'fluffy.'

@MB, many synthetic food colourings are made using benzene as a base. hydrocarbon, carbohydrate, both sound similar, and both store energy. some ‘natural’ colours are made from caramelised sugar, generally the ‘caramel’ colours.


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The Official Kiwi Cultivators Thread

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OfflinePsilosopherr
A psilly goose
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Registered: 02/15/12
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25993079 - 05/14/19 07:30 PM (4 years, 10 months ago)

I'm seeing conflicting answers to the following question and seek clarification brothers: Should I get vented or non-vented petris? Or does it not really matter?

Also, where are you people with the magic links to petri's for $60/case? everything on ebay is like 120 right now

Was very pleased with my first sleeve the other day, I'm really gonna get crackin once I get my case

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OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester... Flag
Last seen: 8 days, 9 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Psilosopherr] * 1
    #25993200 - 05/14/19 08:36 PM (4 years, 10 months ago)

This was posted earlier in the thread https://www.ebay.com/itm/FabLab-100mm-x-15mm-Petri-Dish-Slideable-500-Case-Sterile-FL7694-/163482049744?oid=162944224486

Chaps my ass a little because i spent $90 on my case from Amazon.

Edited by MLPismyOPSEC (05/14/19 08:38 PM)

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MLPismyOPSEC]
    #25993400 - 05/14/19 10:09 PM (4 years, 10 months ago)

Doesn't matter I don't guess... never paid any mind... though I'm pretty sure you've me blocked and won't even see this!
:kittylaugh:

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InvisibleJozxyqk
Other

Registered: 03/30/17
Posts: 21
Re: AGAR ENVY! (Anything and All things agar!) [Re: Psilosopherr] * 1
    #25993595 - 05/15/19 12:51 AM (4 years, 10 months ago)

Quote:

Psilosopherr said:
I'm seeing conflicting answers to the following question and seek clarification brothers: Should I get vented or non-vented petris? Or does it not really matter?






Personal preference I think.  If you wrap your plates then it doesn't really matter. Vented dishes will dry out faster if you don't wrap them. Non vented will last a couple of months without wrapping. I hate wrapping plates, so I like the non vented. The downside is that they tend to be more expensive and harder to find. I'm having a hell of a time finding a good source for them right now and I'm down to my last 8 dishes, oh the horror.

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OfflineBobbit
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Posts: 1,251
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Jozxyqk]
    #25993760 - 05/15/19 04:02 AM (4 years, 10 months ago)

Personally, I ALWAYS wrap petri's or I see edge contamination (or worse).

They need GE, and that requires a barrier unless storing (and viewing) plates in front of an active flow hood, or in a SAB.

I use Glass petri, that aren't 'vented', just irregular enough to allow adequate GE, and the don't dry out for 3-5 months.


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Official New Zealand woodlovers grow thread

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Offlinehexkrak
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Registered: 12/26/02
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25994074 - 05/15/19 09:41 AM (4 years, 10 months ago)


Small spot of Ecuadorian myc I took from a MS inoculated jar. Can't wait to see this pop off over the next few days. 8g agar to 400ml oat boil water.


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Always forgive your enemies; nothing annoys them more. --Oscar Wilde

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OfflineKing0fthajuice
Colors
Male


Registered: 06/28/17
Posts: 325
Loc: Wild Like Volusia in the 80s
Last seen: 1 year, 2 months
Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: hexkrak]
    #25994134 - 05/15/19 10:28 AM (4 years, 10 months ago)

Post deleted by King0fthajuice

Reason for deletion: Dleete


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I like rain

Whether you think you can or you think you can’t, you’re right!

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InvisibleIn Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
Re: AGAR ENVY! (Anything and All things agar!) [Re: King0fthajuice]
    #25994780 - 05/15/19 06:03 PM (4 years, 10 months ago)

Anyone ever see this before?  Dish 4 of 1st round of transfers from a GT clone tissue myc dish transfered on 5/13. This is on LMEA. It is two raised ridges in/on the agar. As you can see the two separate ridges look like a y. I did not put it there nor was it there when I slid the wedge in. Freaky!



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I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!:sun:

Edited by In Lakech (05/15/19 06:56 PM)

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Offlineneef
Old Noob
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Registered: 08/06/12
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Re: AGAR ENVY! (Anything and All things agar!) [Re: In Lakech]
    #25994837 - 05/15/19 06:37 PM (4 years, 10 months ago)

Need some help please with figuring out what are the best parts to transfer...
Some are for previous dish pins that I placed in these dishes and some are transfers from previous dishes 







--------------------
Never the smartest one in the group.
So I keep quite and listen.



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OfflinePsilosopherr
A psilly goose
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Registered: 02/15/12
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Re: AGAR ENVY! (Anything and All things agar!) [Re: neef]
    #25995022 - 05/15/19 07:57 PM (4 years, 10 months ago)

thanks folks.

any reason not to trust ebay agar pre-mixes? Should be all the same stuff eh?

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Offlinehexkrak
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Registered: 12/26/02
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Re: AGAR ENVY! (Anything and All things agar!) [Re: In Lakech]
    #25995125 - 05/15/19 08:50 PM (4 years, 10 months ago)

Quote:

In Lakech said:
Anyone ever see this before?  Dish 4 of 1st round of transfers from a GT clone tissue myc dish transfered on 5/13. This is on LMEA. It is two raised ridges in/on the agar. As you can see the two separate ridges look like a y. I did not put it there nor was it there when I slid the wedge in. Freaky!





Water droplet that moved around as you were rotating the plate looking at it?


--------------------

Always forgive your enemies; nothing annoys them more. --Oscar Wilde

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InvisibleIn Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
Re: AGAR ENVY! (Anything and All things agar!) [Re: hexkrak]
    #25995185 - 05/15/19 09:27 PM (4 years, 10 months ago)

Quote:

hexkrak said:
Water droplet that moved around as you were rotating the plate looking at it?




No I don't think so. The plate is fairly dry and those ridges are solid in the agar. I didn't move the dish around much when I inoculated it either.


--------------------
I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!:sun:

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OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 1,251
Loc: The outsideround Flag
Last seen: 11 days, 11 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: In Lakech]
    #25995395 - 05/16/19 12:59 AM (4 years, 10 months ago)

You'd have to be pretty good to do that on purpose with water drops. .  .


--------------------
The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

–––––    ╭∩╮(-_-)╭∩╮    –––––
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Offlinehexkrak
VisualDecintigrator


Registered: 12/26/02
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Re: AGAR ENVY! (Anything and All things agar!) [Re: In Lakech] * 1
    #25995830 - 05/16/19 09:22 AM (4 years, 10 months ago)

Quote:

In Lakech said:
Quote:

hexkrak said:
Water droplet that moved around as you were rotating the plate looking at it?




No I don't think so. The plate is fairly dry and those ridges are solid in the agar. I didn't move the dish around much when I inoculated it either.



Illuminati confirmed?


--------------------

Always forgive your enemies; nothing annoys them more. --Oscar Wilde

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InvisibleMadHatter333
We Are All Mad Here

Registered: 09/20/17
Posts: 4,650
Loc: Your Mom’s Rabbit Hole
Re: AGAR ENVY! (Anything and All things agar!) [Re: hexkrak]
    #25996726 - 05/16/19 07:39 PM (4 years, 10 months ago)



--------------------

TEKs I Like

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InvisibleIn Lakech
Ala Kin


Registered: 10/09/18
Posts: 131
Loc: this
Re: AGAR ENVY! (Anything and All things agar!) [Re: hexkrak]
    #25996739 - 05/16/19 07:49 PM (4 years, 10 months ago)

Quote:

hexkrak said:
Illuminati confirmed?




Yikes! I better not grow this one out then. If I partake of these fruits they may steal my soul and make me do dark and nefarious things.



--------------------
I will forever miss spirit surfing the multicolored waves of Jerry's guitar riffs!:sun:

Edited by In Lakech (05/17/19 08:05 AM)

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Offlinetranskei
Mr
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Registered: 05/24/07
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Re: AGAR ENVY! (Anything and All things agar!) [Re: In Lakech]
    #25996970 - 05/16/19 10:04 PM (4 years, 10 months ago)

Ghetto plate, but getting closer, PE6

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
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Re: AGAR ENVY! (Anything and All things agar!) [Re: transkei]
    #25997951 - 05/17/19 12:58 PM (4 years, 10 months ago)

Take MUCH smaller transfers and you'll get there MUCH faster...

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