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OfflineBlueAndOrange
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Registered: 01/11/22
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E]
    #27791352 - 05/24/22 10:48 PM (1 year, 9 months ago)

So I’m still struggling to pour agar and not have a ton of condensation. I pour a sleeve per 500ml bottle and work from the bottom up and let them cook in front of the FFU for 24-72 hours before I re-sleeve them. I have a temp gun, and today I poured the first sleeve at 130, second at 120, third at 116. Had 2 broken dishes in one of the sleeves.

Also found my first contaminated plate today. A T1 tidal wave plate that was pretty ugly. The transfer was 9 days ago.

Here is my pouring setup and my ugly plate. It looks like the contaminated plate had the contam dropped on it by the condensation?




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Offlinelen53
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange] * 1
    #27791417 - 05/24/22 11:46 PM (1 year, 9 months ago)

:crazy2::crazy2:

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Offlinenektar61S
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange]
    #27791477 - 05/25/22 12:59 AM (1 year, 9 months ago)

Quote:

BlueAndOrange said:
So I’m still struggling to pour agar and not have a ton of condensation.




Are you putting them in the fridge? I've stopped doing that and get much less condensation.

I pour them, tape a single piece of tape around them in stacks of 10, put the stacks in the sleeves they came in, tape that up. I put them in the corner covered with a clean shirt. Let them sit at least a week to make sure no contam.

Then they can sit 4 to 8 weeks without getting too dry.

Don't put them in a warm grow room, just in a room temp room. 

If you end up with too many and they start getting dry, pour less of them more often.


--------------------
-NEW? Start here.

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OfflineDERRAYLD
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange]
    #27791484 - 05/25/22 01:08 AM (1 year, 9 months ago)

Quote:

BlueAndOrange said:
So I’m still struggling to pour agar and not have a ton of condensation. I pour a sleeve per 500ml bottle and work from the bottom up and let them cook in front of the FFU for 24-72 hours before I re-sleeve them. I have a temp gun, and today I poured the first sleeve at 130, second at 120, third at 116. Had 2 broken dishes in one of the sleeves.

Also found my first contaminated plate today. A T1 tidal wave plate that was pretty ugly. The transfer was 9 days ago.

Here is my pouring setup and my ugly plate. It looks like the contaminated plate had the contam dropped on it by the condensation?








That's metabolites from contamination, if it was from the condensation it would be all over the plate.

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Offlineduvub
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Registered: 02/09/22
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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27791732 - 05/25/22 03:50 AM (1 year, 9 months ago)

Hey everyone!

I'm cleaning p.azurescens LC on agar.
The plan is to create clean, fast growing culture.
The reason why I don't want monoculture is a long growing/fruiting cycle, so I think some genetic variety would be beneficial.


First picture is 12 days ago.
Outlined sectors are for transfer on new plates.
Also, I'm thinking about using one of this sectors for inoculation.

Open for advice and opinions :wink:

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OfflineBlueAndOrange
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27791922 - 05/25/22 07:51 AM (1 year, 9 months ago)

Quote:

nektar61 said:
Quote:

BlueAndOrange said:
So I’m still struggling to pour agar and not have a ton of condensation.




Are you putting them in the fridge? I've stopped doing that and get much less condensation.

I pour them, tape a single piece of tape around them in stacks of 10, put the stacks in the sleeves they came in, tape that up. I put them in the corner covered with a clean shirt. Let them sit at least a week to make sure no contam.

Then they can sit 4 to 8 weeks without getting too dry.

Don't put them in a warm grow room, just in a room temp room. 

If you end up with too many and they start getting dry, pour less of them more often.




I’m not putting them in the fridge. I leave them in front of the FFU to cool and let some of the moisture hopefully evaporate, and then tape them and leave them at room temp. At room temp they condense up as soon as I tape them even if they clear up while they cool.

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OfflineBlueAndOrange
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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27791926 - 05/25/22 07:53 AM (1 year, 9 months ago)

Quote:

DERRAYLD said:
Quote:

BlueAndOrange said:
So I’m still struggling to pour agar and not have a ton of condensation. I pour a sleeve per 500ml bottle and work from the bottom up and let them cook in front of the FFU for 24-72 hours before I re-sleeve them. I have a temp gun, and today I poured the first sleeve at 130, second at 120, third at 116. Had 2 broken dishes in one of the sleeves.

Also found my first contaminated plate today. A T1 tidal wave plate that was pretty ugly. The transfer was 9 days ago.

Here is my pouring setup and my ugly plate. It looks like the contaminated plate had the contam dropped on it by the condensation?








That's metabolites from contamination, if it was from the condensation it would be all over the plate.





Thanks! This is an awesome lesson of the day. Glad I was at least right that it was contaminated as todays trash day and it’s already on the curb.

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OfflineRotnpins
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27791934 - 05/25/22 07:56 AM (1 year, 9 months ago)

I'm curious about some of your guys typical agar procedures.. Do you guys save your old plates after taking transfers from them? Or do you toss them right away?
What is your preferred method of disposing of old plates?
When you go to grain, do you also do a transfer to another plate so you have a genetic copy to go back to?

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InvisibleMysticMycologist
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Re: AGAR ENVY! (Anything and All things agar!) [Re: duvub] * 1
    #27791953 - 05/25/22 08:15 AM (1 year, 9 months ago)

Quote:

duvub said:
Hey everyone!

I'm cleaning p.azurescens LC on agar.
The plan is to create clean, fast growing culture.
The reason why I don't want monoculture is a long growing/fruiting cycle, so I think some genetic variety would be beneficial.


First picture is 12 days ago.
Outlined sectors are for transfer on new plates.
Also, I'm thinking about using one of this sectors for inoculation.

Open for advice and opinions :wink:




When you say you are planning on using them for inoculation, do you mean taking them to grain? Those look like disorganized germ plates to me. You will want to take that a couple more transfers out before going to grain. Your highlighted transfer areas look good to me. Get them on some new plates and see how clean and organized they become.

I like to always wait till at least a T2 before going to grain. Usually a T3 though. Sometimes nasties can hide and become enmeshed in the mycelium on the germ plate and first transfer, and since this is a woodlover, you are going to be investing a lot of time growing this culture, so you might as well make sure it is anexic.

Don’t worry about getting a “monoculture.” You won’t get one through a few transfers. Probably not even after a few dozen. Even the tiniest transfer the size of a needle poke from your MS germ plate will contain a vast amount of strains and genetic diversity. Just focus on getting a clean culture to work with. That is the key.

How did you make you LC? Was it a clone, or MS?


--------------------
Two eyes to look, One eye to see.
Prying open my third eye

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OfflineDendrocopos
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27791995 - 05/25/22 08:49 AM (1 year, 9 months ago)

I have a tendency to hoard plates, but after half my fridge was food and half dishes i decided to man up and start dumping way more.

Unless you're a pheno hunter i think it's perfectly fine to do 1-3 germ plates. Choose the best one, do 1 transfer to 1 plate then another transfer. Spawn. Choose clone. Make like 1-3 master plates and just transfer from those to new plates when you wanna make spawn. I dump all the ms plates.

If you're a paranoid bugger, it's good to think about that the more mycelium coated shit you have in your house the worse for you if you get snagged.

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OfflineDendrocopos
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #27792082 - 05/25/22 09:36 AM (1 year, 9 months ago)


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InvisibleQM33
(NOT A PUPPET!) ❤❤❤❤❤
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos] * 2
    #27792114 - 05/25/22 09:55 AM (1 year, 9 months ago)

Plate pin transfer


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI

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OfflineDERRAYLD
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Re: AGAR ENVY! (Anything and All things agar!) [Re: QM33]
    #27792133 - 05/25/22 10:08 AM (1 year, 9 months ago)

Those twist top pp containers are far clearer than the ones I get.
Looking good.

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Invisiblesandman420
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos] * 1
    #27792168 - 05/25/22 10:30 AM (1 year, 9 months ago)

Quote:

Dendrocopos said:






You trying to set a record on punch size? :crazy2:


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

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Edited by sandman420 (05/25/22 10:30 AM)

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InvisibleQM33
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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27792178 - 05/25/22 10:38 AM (1 year, 9 months ago)

That's with the lid off lol.

Took this pin off coir I spawned in front of the flow in a XLSA after steaming, took it off in front of the flow too.


--------------------
OmManiPadmeHum,OmManiPadmeHum, OmManiPadMeHum...
There are known knowns, there are known unknowns,
          there are also unknown unknowns.
With great privilege comes great responsibility.

Quantom Qups PROOF AND Soft Drops
Turn your Swab to a Syringe and Syringe to Multiple Syringes!
No Pours (QuantomStyal)
Magic Fruit Leather
DMT for IandI

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OfflineDERRAYLD
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Re: AGAR ENVY! (Anything and All things agar!) [Re: QM33]
    #27792198 - 05/25/22 10:55 AM (1 year, 9 months ago)

Oh lid off :lol: .... makes sense now.

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OfflineDendrocopos
Latin woodpecker
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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420] * 1
    #27792369 - 05/25/22 01:44 PM (1 year, 9 months ago)

Quote:

sandman420 said:
You trying to set a record on punch size? :crazy2:




:lol: yeah lawl, i like my punches thicc. I don't buy the whole small transfer narrative unless you have limited culture.

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InvisibleInfinite Monkeys

Registered: 09/01/21
Posts: 276
Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
    #27792535 - 05/25/22 03:58 PM (1 year, 9 months ago)

Quote:

nektar61 said:
Quote:

BlueAndOrange said:
So I’m still struggling to pour agar and not have a ton of condensation.




Are you putting them in the fridge? I've stopped doing that and get much less condensation.

I pour them, tape a single piece of tape around them in stacks of 10, put the stacks in the sleeves they came in, tape that up. I put them in the corner covered with a clean shirt. Let them sit at least a week to make sure no contam.

Then they can sit 4 to 8 weeks without getting too dry.

Don't put them in a warm grow room, just in a room temp room. 

If you end up with too many and they start getting dry, pour less of them more often.




How do you orientate the sleeve when you place it back on the stacks?

Presumably the sleeve goes over the sleeve.

Have you noticed if this reduces condensation compared to wrapping them individually directly after pouring/cooling?

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InvisibleInfinite Monkeys

Registered: 09/01/21
Posts: 276
Re: AGAR ENVY! (Anything and All things agar!) [Re: Dendrocopos]
    #27792539 - 05/25/22 04:00 PM (1 year, 9 months ago)

Quote:

Dendrocopos said:
I have a tendency to hoard plates, but after half my fridge was food and half dishes i decided to man up and start dumping way more.





Surely the solution is to eat only microwavable food and have an entire fridge for dishes? :P

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OfflineDendrocopos
Latin woodpecker
I'm a teapot


Registered: 11/02/21
Posts: 511
Loc: Chained in RR's cellar
Last seen: 1 year, 9 months
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #27792553 - 05/25/22 04:11 PM (1 year, 9 months ago)

I am a little health nut, i don't even own a microwave :P.

I made a "discovery" today. I am currently in the search of a perfect culture and then plan to stop fucking around with spores for a bit. I am also trying to find a way to streamline the process a bit.

I had a syringe. I went two routes.

a) normal syringe - agar - spawn - bulk

b) pf puck with a squirt of spore solution

I expected it, so not the biggest surprise, but maybe some newbies will appreciate it. The process of getting a clone from pf pucks was almost 3 weeks faster than the agar proccess. The benefit of pf puck pins vs agar pins is the fact that you can actually judge the quality of the fruits much better than on agar.

So just a quick tip: if you want a fast clone, try knocking up a pf puck.

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