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 Melliferous
π΅ππ΅ππ΅
Registered: 10/01/20
Posts: 1,053
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 Re: AGAR ENVY! (Anything and All things agar!)  [Re: nektar61]
#27238994 - 03/05/21 04:08 PM (3 years, 1 month ago) |
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It went from an MEA plate to an MEA plate with charcoal and yeast extract. The new nutes definitely tripped it up for a good week. It was so weird seeing the change from a ropey jump off to instant transparency.
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"No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."
Edited by Melliferous (03/05/21 04:09 PM)
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 nektar61
 
Registered: 07/04/20
Posts: 3,271
Loc: Cube Satellite
Last seen: 2 hours, 46 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Melliferous]
#27239004 - 03/05/21 04:14 PM (3 years, 1 month ago) |
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Quote:
Melliferous said:
It went from an MEA plate to an MEA plate with charcoal and yeast extract. The new nutes definitely tripped it up for a good week. It was so weird seeing the change from a ropey jump off to instant transparency.
thank you.
Does charcoal act as a nute? I thought it was just for that goth charm. Someone said mycelium eats food coloring but not charcoal.
Do you think it was mostly the yeast extract?
Do you think it was transferring from one nute to another? If so, that's worth some experimentation. Let me know please what thread to sub if you start on that!
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clockworkshroom
Stranger
Registered: 02/20/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Melliferous]
#27239005 - 03/05/21 04:14 PM (3 years, 1 month ago) |
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Do you think there is any chance this is APE myc? It's almost as high as it is wide, it's on a obviously very contaminated plate, it doesn't look like it should and it's not where I swabbed.... but it is uniform, it is eating the food dye and it is growing at myc speed, no quicker.
It looks nothing like healthy myc I've seen before but it's from one of the swabs Roarkell kindly sent me and I would love to save one so if there is even a 1% chance I'll transfer.
   
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nektar61
Registered: 07/04/20
Posts: 3,271
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Re: AGAR ENVY! (Anything and All things agar!) [Re: clockworkshroom]
#27239013 - 03/05/21 04:19 PM (3 years, 1 month ago) |
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Quote:
Melliferous said:
It went from an MEA plate to an MEA plate with charcoal and yeast extract. The new nutes definitely tripped it up for a good week. It was so weird seeing the change from a ropey jump off to instant transparency.
thank you.
Does charcoal act as a nute? I thought it was just for that lovely visual goth charm for plate porn pics.
Someone said mycelium eats food coloring but not charcoal.
Do you think it was mostly the yeast extract?
Do you think it was transferring from one nute to another? If so, that's worth some experimentation. Let me know please what thread to sub if you start on that!
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 A.k.a
Stranger
Registered: 10/27/19
Posts: 16,965
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Re: AGAR ENVY! (Anything and All things agar!) [Re: clockworkshroom]
#27239015 - 03/05/21 04:21 PM (3 years, 1 month ago) |
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That looks fine to me.
Itβs just a little tomentose, plus germ plates look weird a lot of the time.
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 LAGM2020      
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clockworkshroom
Stranger
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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a] 1
#27239033 - 03/05/21 04:38 PM (3 years, 1 month ago) |
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Thanks AKA it looks weirdly less suspect in the photos than it actually does. It's really fluffy and round. I'll definitely transfer away though and see what happens.
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nektar61
Registered: 07/04/20
Posts: 3,271
Loc: Cube Satellite
Last seen: 2 hours, 46 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
#27239115 - 03/05/21 05:35 PM (3 years, 1 month ago) |
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Quote:
A.k.a said:
....plus germ plates look weird a lot of the time.
Is a germ plate the plate you germinate spores on, as opposed to the one you transfer to?
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I have a general question about agar, do you have to use something with FAE to tape Petri dishes shut (micropore, parafilm), or can you use a non-porous clear packing tape?
Can knocked-up plates be stuck in a box in a closet, or do they have to "breathe" a bit?
thank you
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Crackatoa
Stranger in a strange land
Registered: 03/31/19
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
#27239174 - 03/05/21 06:08 PM (3 years, 1 month ago) |
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Little Fuckin satellite, best looking rhizo I've in a min. Transferring tonight, hopefully I can keep the rhizo going. I got a nice looking backup though.
3 o'clock
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D3_Myc
Weeb Trash
Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa] 1
#27239212 - 03/05/21 06:21 PM (3 years, 1 month ago) |
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What about in the myc at 11 is that just a reflection
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joze
Registered: 11/10/20
Posts: 1,050
Loc: PNW
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] 1
#27239223 - 03/05/21 06:25 PM (3 years, 1 month ago) |
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Quote:
nektar61 said:
Quote:
A.k.a said:
....plus germ plates look weird a lot of the time.
Is a germ plate the plate you germinate spores on, as opposed to the one you transfer to?
-
I have a general question about agar, do you have to use something with FAE to tape Petri dishes shut (micropore, parafilm), or can you use a non-porous clear packing tape?
Can knocked-up plates be stuck in a box in a closet, or do they have to "breathe" a bit?
thank you
Yeah a germ(ination) plate is the one you germinate spores on. In regards to the FAE, a lot of people use saran wrap/cling wrap on their dishes with great success. I don't see why packing tape wouldn't work, but it would be annoying to take off when you want to work with the plates. They technically do need to breathe a bit, but if they're growing slowly, the rate of oxygen consumption drops off pretty drastically. At low temperatures you can probably store them without any fresh air for a pretty long time, like a couple months maybe? I know a lot of people have good success storing cultures in the fridge long term.
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Crackatoa
Stranger in a strange land
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
#27239267 - 03/05/21 06:45 PM (3 years, 1 month ago) |
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Quote:
D3monic said:
What about in the myc at 11 is that just a reflection
Yeah, I had to double check.
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tedoro
ToadStool Tender
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa] 1
#27239338 - 03/05/21 07:23 PM (3 years, 1 month ago) |
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Packing tape isn't flexible... I doubt it would work.
In respect to adjusting to new nutes... my agar drops are taking longer to leap off in my millet bags than in my wheat bags, could that be a reason? (agar & LME plates)
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC
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nektar61
Registered: 07/04/20
Posts: 3,271
Loc: Cube Satellite
Last seen: 2 hours, 46 minutes
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Re: AGAR ENVY! (Anything and All things agar!) [Re: joze]
#27239343 - 03/05/21 07:26 PM (3 years, 1 month ago) |
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Quote:
joze said:
Quote:
nektar61 said:
Quote:
A.k.a said:
....plus germ plates look weird a lot of the time.
Is a germ plate the plate you germinate spores on, as opposed to the one you transfer to?
-
I have a general question about agar, do you have to use something with FAE to tape Petri dishes shut (micropore, parafilm), or can you use a non-porous clear packing tape?
Can knocked-up plates be stuck in a box in a closet, or do they have to "breathe" a bit?
thank you
Yeah a germ(ination) plate is the one you germinate spores on. In regards to the FAE, a lot of people use saran wrap/cling wrap on their dishes with great success. I don't see why packing tape wouldn't work, but it would be annoying to take off when you want to work with the plates.
thank you.
Is food cling wrap porous? I assume packing tape is not?
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Nichrome
Pariah
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61]
#27239425 - 03/05/21 08:15 PM (3 years, 1 month ago) |
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Quote:
nektar61 said:
Quote:
Nichrome said:
Trying to diagnose mold vs cube without a microscope is like trying to learn history from a religious text.
ha!
Quote:
Nichrome said:
You can tell the difference between Fungi Imperfecti vs Fungi Perfecti by the way it forms. Mold is a bunch of individuals that live together and run next to each other. Higher fungi intertwine and join and split and rejoin. You can see that in that photo with the naked eye although without experience in mycroscopy it would be hard to tell the difference with the naked eye. For sure you have some kind of higher fungi (mushroom) mycelium and not mold.
Here's a photo I took, cube spores on agar and what looks like cube mycelium. Do you confirm?
I've yet to take a good pic of mold mycelium next to cube mycelium. But I circled in red what I think may be 2 bacterium. What do you think?
thank you
You have a bunch of stuff going on there that is not cube mycelium.
This is healthy cube germination...
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Where have all the people gone my honey
Where have all the people gone today
There's no need for you to be worrying about all those people
You never see those people anyway
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tedoro
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
#27239490 - 03/05/21 08:49 PM (3 years, 1 month ago) |
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And if I was to change the nutes in my agar... what could I use that is more millet-like? (that is repeatable and measurable)
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC
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Crackatoa
Stranger in a strange land
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tedoro] 1
#27239521 - 03/05/21 09:11 PM (3 years, 1 month ago) |
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Millet grain soak water, you could probably put some millet in a food processor and use it instead of brf in brf agar.
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tedoro
ToadStool Tender
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
#27239748 - 03/05/21 11:16 PM (3 years, 1 month ago) |
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I just saw that a local mill (bob's red mill) makes a millet flour. Could I try the same weight of millet flour in the stead of the LME?
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Deep pour soft agar plates-->bags of WBS-->Low Profile Monos
Clean spawn thread | Put a thermometer on your PC
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kanemush
Grumpy
Registered: 12/07/17
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
#27239754 - 03/05/21 11:28 PM (3 years, 1 month ago) |
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The Fresh Prints
Smell ya later
Registered: 05/19/12
Posts: 1,377
Loc: Bel-Air
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Re: AGAR ENVY! (Anything and All things agar!) [Re: kanemush] 1
#27239760 - 03/05/21 11:41 PM (3 years, 1 month ago) |
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Love it when fugly germ plates start throwing pins.
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Zakkery
Registered: 10/15/18
Posts: 299
Loc: UK 
Last seen: 2 hours, 29 minutes
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One of those looks meaty, clone candidate?
Also what the fuck is the deal with those bubbles? That normal on old funky petris?
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