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InvisibleD3_Myc
Weeb Trash
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Registered: 05/06/18
Posts: 4,399
Loc: Year Zero
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii] * 3
    #27054671 - 11/23/20 09:54 PM (3 years, 4 months ago)




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OfflineThe Dalcassian
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rafiikii]
    #27054761 - 11/23/20 11:10 PM (3 years, 4 months ago)

Ya APE being a pain to germinate isn't uncommon. Pretty sure pasty said once it's clean slant it and never do it again lol


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InvisibleD3_Myc
Weeb Trash
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Registered: 05/06/18
Posts: 4,399
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Re: AGAR ENVY! (Anything and All things agar!) [Re: The Dalcassian]
    #27054766 - 11/23/20 11:15 PM (3 years, 4 months ago)

I’ve burned through 5 swabs and got nothing. A spec of bacteria that was it.

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Offlinelookintolearn
Stranger becoming Strangest
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Registered: 06/02/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: joze]
    #27054788 - 11/23/20 11:49 PM (3 years, 4 months ago)

Thanks man! Wish I would get to using petris so I could take better pics lol. I think honestly I'm going to put that first Argentinian plate to some grains tomorrow since the growth is looking so solid and those KSSS i'm trying to semi-isolate the best growth until they are perfect.

I'm thinking all my KSSS plates i'll keep transferring till about 20 or so and then see what they are looking like. Probably not going to get a perfect isolate but we shall see. They are the UFO phenotype so I want to find the clone with the best saucers haha.


And D3monic gah damn all your agar always looks so pristine. Make me slobber  :homerdrool:


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI

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InvisibleRafiikii
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27054829 - 11/24/20 12:54 AM (3 years, 4 months ago)

Quote:

The Dalcassian said:
Ya APE being a pain to germinate isn't uncommon. Pretty sure pasty said once it's clean slant it and never do it again lol



Quote:

D3monic said:
I’ve burned through 5 swabs and got nothing. A spec of bacteria that was it.




good to know. thanks.

D3monic changing the thread from agar envy to straight agar porn.

:three:


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InvisibleSunny Skies
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27054976 - 11/24/20 06:31 AM (3 years, 4 months ago)

the TAT ..is that a True albino teacher?

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InvisibleD3_Myc
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Sunny Skies]
    #27055041 - 11/24/20 07:55 AM (3 years, 4 months ago)

Quote:

Sunny Skies said:
the TAT ..is that a True albino teacher?




Yes

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InvisibleSunny Skies
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27055115 - 11/24/20 08:55 AM (3 years, 4 months ago)

very nice,
Im trying my luck with the same variety from a swab..fingers crossed for germination.

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Invisiblejoze
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Re: AGAR ENVY! (Anything and All things agar!) [Re: lookintolearn]
    #27055200 - 11/24/20 09:41 AM (3 years, 4 months ago)

Quote:

lookintolearn said:
Thanks man! Wish I would get to using petris so I could take better pics lol. I think honestly I'm going to put that first Argentinian plate to some grains tomorrow since the growth is looking so solid and those KSSS i'm trying to semi-isolate the best growth until they are perfect.

I'm thinking all my KSSS plates i'll keep transferring till about 20 or so and then see what they are looking like. Probably not going to get a perfect isolate but we shall see. They are the UFO phenotype so I want to find the clone with the best saucers haha.





Good luck in your search for the best saucers, I'm looking forward to seeing how things play out for you.


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OfflineRumpleforeskin
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Re: AGAR ENVY! (Anything and All things agar!) [Re: joze]
    #27055508 - 11/24/20 01:07 PM (3 years, 4 months ago)

I've been getting really light and whispy tomentose growth on my plates.  I used grain water as my nutrient base.  I'm up to T6 on a few of my transfers and still little to no rhizomorphs growth.  All of the isolations so far have been from MSS, as I'm still waiting got first fruits to clone something nice. 
I've now moved to LME and Agar (10g of each to 500 mL).  Will this increase the likilihood of changing the growth pattern of the mycelium?
Growth appears clean, but not as textbook as I see there. 
Also - I accidentally bought 47 mm plates.  They obviously colonize faster.  How long do you wait to make a transfer on average?

Thanks.

Edited by Rumpleforeskin (11/24/20 01:08 PM)

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InvisibleD3_Myc
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rumpleforeskin]
    #27055542 - 11/24/20 01:30 PM (3 years, 4 months ago)

Depends on how the plate looks some xfer at dime size, I usually get around to it at silver dollar sized later xfers might not take place until the myc is getting close to the edge

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Offlinelookintolearn
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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #27055631 - 11/24/20 02:35 PM (3 years, 4 months ago)

Thanks Joze! I'll keep it updated as I keep testing some of these plates. I think I'll make 3-4 20qts with some of my best plates and see what goes.

And Rumple it seems to me that you aren't always going to get rhizo growth with every culture. I've especially noticed this with my KSSS, I have about 7-8 clones and not a single one has rhizo growth, all of them are pretty wispy but they still have been working well. I'm on T7 on about 5 of them and haven't seen any signs of that pretty ropey goodness. But tomentose growth isn't bad at all just sometimes makes spotting bacteria harder and not as picture pretty lol.


--------------------
Don't be afraid of feeling the feelings

Lookin to LAGM 2021



Looking to start growing? Read through Bod's Introduction to Everything
Looking to start agar? Start with Alien's Holy Grail
Looking to perfect your transfers? Start with D3monic's Perfect Transfers
Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek
Looking to start LC? Try LI first! Munch's super easy Blenderless LI

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OfflineDrunkUncle
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Registered: 08/09/20
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Re: AGAR ENVY! (Anything and All things agar!) [Re: lookintolearn]
    #27055674 - 11/24/20 03:01 PM (3 years, 4 months ago)

First attempt at using Agar plates. These are all germination plates.I had no clue what I was doing on the first few, then found C10's write up and did about 40 more plates using the streak technique outlined there. You can't see, but on some I used my full r-tard strength and tore the shit out of the agar (lol).

The TOC spores are from a TC (Thanks again), the UKC(Unknown Cubensis)is from my first monotub that I took prints from, and the Tx Syringe is from a local homie of mine. Definitely had some blue green weirdness on a few of the UKC plates and tossed them, hoping that some of these are okay to do some transfers in a bit, hoping for some input from ya'll








--------------------
OEF Combat Medic & Purple Heart Recipient

“If you get the message, hang up the phone. For psychedelic drugs are simply instruments, like microscopes, telescopes, and telephones. The biologist does not sit with eye permanently glued to the microscope, he goes away and works on what he has seen.”

― Alan Watts


Smartattack said:

I was doing Tai chi while flying through space, took a shit in my shower, removed my face several times with my hands and that was all just the boring part before it really hit me.:eek::eek:

Edited by DrunkUncle (11/24/20 03:04 PM)

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OfflineRumpleforeskin
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Re: AGAR ENVY! (Anything and All things agar!) [Re: lookintolearn]
    #27055765 - 11/24/20 03:54 PM (3 years, 4 months ago)

Thanks for the response.  You as well, D3mon...
If nothing else, I'm getting lots of practice in before I start with tissue cultures and Master/Slave management. 
Gone through 75 plates in 2 months just farting around with transfers and Tiger dropping clean agar into new jars. 
Lots of fun learning.

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Offlinetryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rumpleforeskin]
    #27055776 - 11/24/20 04:00 PM (3 years, 4 months ago)

Tomentise myc usually comes from too nutritious of a recipe.

I would dilute your grain water or use less lme


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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OfflineRumpleforeskin
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #27055780 - 11/24/20 04:02 PM (3 years, 4 months ago)

Thank you.  I was also wondering this.  Interested to see if using LME instead of grain water will help.  Also dying the agar blue, you know because it looks more awesome.

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Invisiblemushhead
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rumpleforeskin]
    #27055795 - 11/24/20 04:13 PM (3 years, 4 months ago)

Quote:

Rumpleforeskin said:
Thank you.  I was also wondering this.  Interested to see if using LME instead of grain water will help.  Also dying the agar blue, you know because it looks more awesome.



Grain water works fantastically if you boil it and sterilize it proper.
I dilute mine down a BUNCH with distilled water.
LME agar is also a great choice. I do 2.5g LME to 500ml of distilled water.
Everyone has their own recipe and some use low nutes for plates to get that really nice rhizo growth.

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Offlineroarkell
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushhead] * 1
    #27055804 - 11/24/20 04:28 PM (3 years, 4 months ago)


Besides the darker blue plates (slow ass Jalisco) all these are today's transfers... all sorts of new goodies!

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InvisibleD3_Myc
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Re: AGAR ENVY! (Anything and All things agar!) [Re: roarkell]
    #27055820 - 11/24/20 04:35 PM (3 years, 4 months ago)

What are you using for the dark blue dye?

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OfflineSped105
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #27055823 - 11/24/20 04:38 PM (3 years, 4 months ago)

Quote:

tryptkaloids said:
Quote:

GrinchGrower said:
I am very new at this still so a little uncertain what to look/smell for



Only time your nose will help you is right before spawning.  Smelling plates tells you nothing.
Ambrose, variety? Looks like a mutant mushroom.




Who's smelling plates?

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