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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28573290 -

I like that take on the subject better than mine.
Positive outlook!!!
:dancer:


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Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW]
    #28573640 -

Do any of you use nutritional yeast in your Agar mixture? What are the differences you notice between using it and not?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Takeatrip] * 1
    #28573667 -

I use nutritional yeast in a normal LME mixture. Before using yeast I was getting weak and transparent growth on agar. Cultures would perform perfectly fine on grain and when spawned, but agar growth wasn't great. It was annoying but I dealt with it for a long time.

Since adding 1g of NY on a recommendation, I'm getting what I would consider vastly more robust growth. The mycelium is a brighter white, more dense, and I seem to get rhizomorphic cultures more.

My stance on it is that if you are having issues with weak, whispy, or transparent growth, you should definitely give it a shot. I'm not the only person who has had this experience.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Way]
    #28573731 -

Way said:
I use nutritional yeast in a normal LME mixture. Before using yeast I was getting weak and transparent growth on agar. Cultures would perform perfectly fine on grain and when spawned, but agar growth wasn't great. It was annoying but I dealt with it for a long time.

Since adding 1g of NY on a recommendation, I'm getting what I would consider vastly more robust growth. The mycelium is a brighter white, more dense, and I seem to get rhizomorphic cultures more.

My stance on it is that if you are having issues with weak, whispy, or transparent growth, you should definitely give it a shot. I'm not the only person who has had this experience.


Ok, thanks for your input. I have ordered some in flake form. Would you use a pestle and mortar to grind it into yeast powder so it dissolves better?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Takeatrip]
    #28573750 -

If I was smart enough, probably. I use it in flake form and just do my best to dissolve it. I usually still get some sediment but I don't care too much about crystal clear agar. If you care about clarity, it seems like a good idea.


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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Way]
    #28574029 -

I had weak looking growth on my last round of lme transfers.

I just switched to pda. :shrug:


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 2
    #28574828 -

I’ve never seen ropes just stand straight up in the air like this before. Tidal wave




And trypt, you better not tell me it’s a bad thing 😂 every time I think something good is happening, you tell me it’s bad and why 🤦‍♂️ 😂

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28574841 -

Pretty sure that’s a flerken.





--------------------

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: HILLBILLY OUTLAW]
    #28574848 -

:tryingnottodie::feelsparanoidman:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #28574946 -

SupaThaRipper said:
I’ve never seen ropes just stand straight up in the air like this before. Tidal wave




And trypt, you better not tell me it’s a bad thing 😂 every time I think something good is happening, you tell me it’s bad and why 🤦‍♂️ 😂



There's an interview with Alan where he talks about Tidal Wave having crazy vertical rhizomorphs that he hadn't seen before... I think... or are they called hyphae. Idk im drunk

NVM found the interview he's talking about TW2 under a scope


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Edited by Pnin (12/08/23 08:27 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pnin] * 3
    #28574983 -

Idk I’m drunk 🤣 🤣

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #28575047 -

SupaThaRipper said:
I’ve never seen ropes just stand straight up in the air like this before. Tidal wave






Dude, you have summoned Cthulhu! :eek: Make sure he doesn't run away from the dish! :omgawesome:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bra] * 1
    #28575050 -

I trapped him in a microcentrifuge! He’s contained.. for now 😂

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PhenoDreamers]
    #28575053 -

SupaThaRipper said:
I trapped him in a microcentrifuge! He’s contained.. for now 😂



:pointmade:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bra] * 2
    #28576974 -

These were transferred from the same plate.  The teal plate is just MEA at 1.2% and the purple plate is 1.2% MEA + yeast.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kinoko314]
    #28576987 -

Kinoko314 said:
These were transferred from the same plate.  The teal plate is just MEA at 1.2% and the purple plate is 1.2% MEA + yeast.





Its posts like this that make me wonder what Im doing wrong. I use 1.5% LMA and although its clean, I only get very light transparent growth.





Nats look good though



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Edited by AspectOfTheCreator (12/10/23 12:45 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28576993 -

my LME agar looked whispy & weak as shit until i started adding yeast. try a small bit of nutritional or active yeast (i use active & it works great ime) in your recipe. i use d3’s LME agar recipe & follow it pretty much exactly. 1g yeast to 1000ml agar


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you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

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Re: AGAR ENVY! (Anything and All things agar!) [Re: hazyhorse]
    #28576994 -

hazyhorse said:
my LME agar looked whispy & weak as shit until i started adding yeast. try a small bit of nutritional or active yeast (i use active & it works great ime) in your recipe. i use d3’s LME agar recipe & follow it pretty much exactly. 1g yeast to 1000ml agar



Ive used yeast but it only worked marginally and left a bunch of sediment in the plates so I stopped using it.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28577004 -

I've struggled to get a lot of good rhizo growth.  Usually I get tomentose even if it's clean.  That's why I've lowered it down to 1.2% nutes.  On most of my cultures the yeast caused super fluffy growth.  The lower nutes helped, but also it seemed to get better as I kept transferring, even though I felt more transfers weren't necessary.

I don't know if any of that will help you, but good luck in your agar adventures.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 1
    #28577013 -

AspectOfTheCreator said:
Ive used yeast but it only worked marginally and left a bunch of sediment in the plates so I stopped using it.



weird, yeast really turned my plates around. if you try it again in the future, you can decant the liquid off or use a french press so the sediment gets left behind


--------------------
you're not the first to set foot here, just another
===================================
i love glass petris & you can too!!
posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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