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 Digit
Surreal Estate Agent
Registered: 08/10/15
Posts: 70
Loc: tierra incognita
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Hey folks,
I'm putting my lab area back together after a 2 year break and I'm looking at my tiny 12x12 flow hood with a bit of dread in having to work in such a small area. I did it before, and although it was a major PITA it worked out ok anyway. Now I'm wondering if I should spring for a larger filter and build a new hood.
Do any of you guys use a 12x12 FH for agar work?
I live in a remote area outside of the continental US so shipping costs are insane and a new filter would probably cost more to ship here than the cost of the filter itself.
Any suggestions?
Thanks, I can't wait to start posting some plate pics.
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Question everything.
Believe nothing.
Think for yours3lf.
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mushboy 
modboy
Registered: 04/24/05
Posts: 32,776
Loc: where?
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 Re: New to agar  [Re: Digit] 1
#26357475 - 12/02/19 04:07 PM (4 years, 4 months ago) |
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Take the pic at an angle so you dont get the reflection. Directly overhead gives you flash reflections or the outline of your phone.
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 A.k.a
Stranger
Registered: 10/27/19
Posts: 16,965
Loc: Gaming the system
Last seen: 40 seconds
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Re: New to agar [Re: mushboy]
#26357640 - 12/02/19 05:56 PM (4 years, 4 months ago) |
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How do you guys get rid of condensation?? I just poured my first plates the other day and it seems like no matter what I do a couple always have it.
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 LAGM2020      
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 MH5109
Stranger
Registered: 09/27/17
Posts: 1,385
Loc: America 
Last seen: 3 years, 2 months
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Re: New to agar [Re: A.k.a] 1
#26357757 - 12/02/19 07:08 PM (4 years, 4 months ago) |
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I flip mine upside down and poke a couple holes in the parafilm to let it escape. Iv had the same issue ever since i started pouring plates, even when the agar is cool, under 110°f it still condensates on the lid.
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Feasoghorm
Registered: 10/24/18
Posts: 4,384
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 Re: New to agar [Re: MH5109]
#26357833 - 12/02/19 07:46 PM (4 years, 4 months ago) |
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The optimal pour temp is 117F. Stacking the plates while cooling will assist in preventing condensation on the plates being covered. However the top (and sometimes bottom) plate(s) usually wind up fucked. After 2wks of sitting wrapped in my desk drawer they ususally clear up.
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daysbetween
Stranger
Registered: 10/23/19
Posts: 73
Last seen: 1 month, 2 hours
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I always put a cup of warm water on top of the stack while they set... never have any condensation that way.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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I just pour ahead of time, usually dissipates prior to use
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trubblesome
Stranger
Registered: 11/09/19
Posts: 406
Last seen: 1 year, 1 month
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yep, stack plates higher so you have fewer top and bottom plates, basically. setting something warm over those plates will clear them up too if you need the plate and it's bothering you.
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Caps McGee
Grandaddy Smurfshack
Registered: 10/28/17
Posts: 14,357
Loc: ally known as ...
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Never understood the big issue muself... I mean, "visibility" meh... its water... "spreads infection"... shouldn't be there to start with lol... lower temps, higher stacks, pouring ahead, even pouring in an overheated space so it doesn't cool too quickly...
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MH5109
Stranger
Registered: 09/27/17
Posts: 1,385
Loc: America
Last seen: 3 years, 2 months
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Iv tried stacking plates with a heat mat under my sab and the top 4 or so plates always no matter what have condensate on them. It does usually go away if i leave them for a few days, but flipping them after inoculation keeps mine pretty clear.
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nevereataamanita
Registered: 09/08/19
Posts: 121
Last seen: 1 month, 22 days
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Re: New to agar [Re: MH5109]
#26358007 - 12/02/19 09:25 PM (4 years, 4 months ago) |
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Quote:
Samiam76 said: What is the best way to take photos of these things?
I've been struggling with this, too, I think the best thing is light and lots of it--lately I've been getting the best results by placing the plate on a flat surface of a contrasting color (like, dark compared to the white mycelium) directly in front of a window with daylight coming through. Then I turn on every light in the room, and shine my grow light on the plate, too. For plates with condensation issues, I've also been having some luck with shining the grow light at the plate from the side at a low angle. I think the light coming in through the side of the petri dish is what makes it turn out better. The first plate below had really bad condensation, but by shining the light from the side I could get an OKAY photo. The condensation really cleared up by the time I took the second picture, which is a lot nicer (satellite contamination still a problem, tho).
 
EDIT: Oh, and as others have noted, if you're using your phone try taking the photo from further away (18-24 inches?) and then crop. A lot easier to get good focus.
Edited by nevereataamanita (12/02/19 09:28 PM)
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A.k.a
Stranger
Registered: 10/27/19
Posts: 16,965
Loc: Gaming the system
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These are my first ever plates, from three days ago. I can’t believe how fast they grow.
I took pics from the back cuz there’s so much condensation so they’re kinda blurry.
I took two samples each from two fruits, the goal is to try to get one of those crazy canopies. So how long do I wait to start taking transfers, and where do I go from here??
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LAGM2020
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SunnyDayze
Finder of shrubberies
Registered: 12/30/17
Posts: 2,230
Loc: Canada
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Re: New to agar [Re: A.k.a]
#26359903 - 12/03/19 08:07 PM (4 years, 4 months ago) |
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They’re looking good! Anytime really but I’d let them grow another day or two and take early transfers. Middle left is lookin fine  middle right 
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curious.psychonaut
Stranger
Registered: 10/17/19
Posts: 282
Last seen: 4 years, 2 months
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Quote:
Samiam76 said:
What is the best way to take photos of these things? Backlighting?
Best I've found is with an external flash, if you happen to have one lying around. Point it towards the ceiling & shoot directly over dish. If there's a reflection, swivel the flash a bit & try again.
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My LAGM2020 grow log
  
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Grimsweeper
don't fear the sweeper
Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 1 month, 21 days
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Time for cold storage to serve again another day. Thank you for your service..
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When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps
  
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rickyswamps
Bad Apple
Registered: 11/08/18
Posts: 1,192
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Quote:
Grimsweeper said:
Time for cold storage to serve again another day. Thank you for your service..
How are you storing those to keep them looking so good? I rarely feel comfortable using them directly out of the fridge because of all the condensation.
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BigSurMoon
the deathless ones
Registered: 03/21/18
Posts: 876
Loc: Chicken coop
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Quote:
rickyswamps said:
Quote:
Grimsweeper said:
Time for cold storage to serve again another day. Thank you for your service..
How are you storing those to keep them looking so good? I rarely feel comfortable using them directly out of the fridge because of all the condensation.
I think they havent been in the fridge yet, I cold store plates in the fridge upsidedown, in a plastic bag that's not sealed up. I do this so if there is crazy condensation or pooling, its not drowning the culture. And if something does get flooded, it doesnt always mean the culture is fucked.
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My soul brings tears, to angelic eyes. DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1
Deliberately asleep, always.
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rickyswamps
Bad Apple
Registered: 11/08/18
Posts: 1,192
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Quote:
BigSurMoon said:
Quote:
rickyswamps said:
Quote:
Grimsweeper said:
Time for cold storage to serve again another day. Thank you for your service..
How are you storing those to keep them looking so good? I rarely feel comfortable using them directly out of the fridge because of all the condensation.
I think they havent been in the fridge yet, I cold store plates in the fridge upsidedown, in a plastic bag that's not sealed up. I do this so if there is crazy condensation or pooling, its not drowning the culture. And if something does get flooded, it doesnt always mean the culture is fucked.
Gotcha. They are usually fine with water in there. But I take a transfer to see. Would rather be able to put good looking plates in the fridge and pull them out later to go directly to grain.
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Grimsweeper
don't fear the sweeper
Registered: 01/29/18
Posts: 4,843
Loc: broom closet
Last seen: 1 month, 21 days
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Correct these havent been in the fridge yet. I keep them stacked in a gallon ziplock not completely sealed.its a small wine fridge with a lot of air movement so I think that helps keep condensation down.
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When you clean a vacuum cleaner you are a vacuum cleaner
Build yourself a Flow Hood in these 99 simple steps
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TonyMushrana
is that a Trich again?
Registered: 03/12/19
Posts: 323
Loc: Mountainly Place
Last seen: 2 years, 1 month
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Hei guys!
Made some clones from cluster and getting strange growth? Any tips what the hell is going on?
Looks like nice mycelium growth inside, and this special ring of weak or wtf growth
Thanks, Tony
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