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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 2
    #25662461 - 12/06/18 07:11 PM (5 years, 1 month ago)

EXPANSION!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MrFahrenheit]
    #25665120 - 12/08/18 07:38 AM (5 years, 1 month ago)

Quote:

DarkyDonno said:
Well I just got my first isolate of Orissa India, I think I did 4 clones to agar and 3 have obvious sectoring but the fourth is a perfect circle. I transferred to two more plates so hopefully I can use it by next week. Will post pics when I'm home.



May not be thinned out enough to show sectors yet... chances of isolation directly from clone or slim to none IME... got any pictures?


Quote:

Shroomspective said:
Here's some APE on three plates enjoying itself on PDYA..Note the matt patches are external to the lid, not bacterial blooms, they're nice and clean G4's







Bacteria wouldn't be fuzzy... but mold would


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Re: AGAR ENVY! (Anything and All things agar!) [Re: yamo25000]
    #25672513 - 12/11/18 02:18 PM (5 years, 1 month ago)

Quote:

ShaperDreaming said:
Quote:

Organic_Magic said:
:obamafrown:

I know you said those black spots are spores and I really want to believe you buuuut.......

Ima have to wait to see how this turns out.



It was in a syringe that came like this, and the big glob shot out suddenly too quickly to do shit about it. I have a lot of syringe experience (not mycology) so I wasn't expecting it to be able to... do that:


Quote:

Shroomspective said:That's not quite how agar works..you need to base it on requirements..Potato water is fine to use, about 150g of potato boiled, or 4.5g flakes, in a little water, filtered and the made up to 500ml, but you'd only want to add about 1g of simple sugar, such as honey to that


Good luck tho:thumbup:



Thanks! I think it's doing the trick for now. Also, this is why I only poured 5 plates :wink: The recipe I looked at seemed equivalent to this, but called for corn syrup, and this was best I could do in the moment.

Just looking to isolate a solid strain of PE so I can move it over to grains. I know these guys are slow in a lot of ways, so I thought science would get me there.



Always shake your syringe well before flame sterilizing...
Quote:

yamo25000 said:
I have a few questions about agar. I've read lots of teks and I've worked with it a bit myself, but I feel like I may be doing things wrong.

I've never had a petri dish be successful. Every time it gets bacteria or mold. I prepared PDA, pc'd it, and poured it in a SAB, but I didn't wrap them after pouring them, and would often shake them to get moisture off the lid. Could that have been my issue?

Another question regarding the sterilization of agar: If it's possible to sterilize glass petri dishes, is it not possible to sterilize pre-poured plates in glass petri dishes? I've seen a tek that shows how to sterilize the glass dish, but only before agar is poured onto it.

When people use petri dishes for agar, is it common practice to wrap them in plastic, or put them in baggies to eliminate airflow?



Not a possibility, a high probability that that's your issue... I'm sure you could pour then sterilize, just allow them to cool(solidify) overnight... also, wanna wrap stacks of dishes well in tinfoil, so you can get them from pc to SAB without comtaminating... I cut saran wrap into inch thick rolls and wrap dishes that way... I keep them in 12/12 lighting on a closet shelf... not trying to eliminate air flow, as saran wrap is permeable and allows molecular gas exchange... just keeps out the nasties


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25672555 - 12/11/18 02:39 PM (5 years, 1 month ago)

:goodluck:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Johnny Dont]
    #25672591 - 12/11/18 02:55 PM (5 years, 1 month ago)

Give it a shot and let us know!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: transkei]
    #25685268 - 12/17/18 12:15 PM (5 years, 1 month ago)

You can still work with 10:30ish


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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25691551 - 12/20/18 12:14 PM (5 years, 1 month ago)

I've been using 4.5g agar powder and 7g LME to 500ml of water... makes the agar soft and wet: ideal for spore germination and SAGARI 


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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25691694 - 12/20/18 01:31 PM (5 years, 1 month ago)

It essentially acts as an LI, as it liquifies on the initial shake... the recipe has worked for all applications IME


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Failboat]
    #25692419 - 12/20/18 07:08 PM (5 years, 1 month ago)



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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25696675 - 12/22/18 06:18 PM (5 years, 1 month ago)

LC to agar works yes... in fact, it's recommended to ensure cleanliness of the LC


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25697828 - 12/23/18 09:52 AM (5 years, 1 month ago)

:whathesaid:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mike O.Rrhizal]
    #25703384 - 12/26/18 08:42 AM (5 years, 1 month ago)

Nice! Still prefer to pour... taking the mad scientist out of it


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ImpishShroom]
    #25703405 - 12/26/18 08:54 AM (5 years, 1 month ago)

I'd fruit, pick 5 to clone, and isolate from your fastest culture


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703629 - 12/26/18 11:17 AM (5 years, 1 month ago)

You just streak the swab like you would a loop: I've seen people pull the cotton off with APE bc the spores are clear... idk lol


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Re: AGAR ENVY! (Anything and All things agar!) [Re: sporealchemy]
    #25703657 - 12/26/18 11:33 AM (5 years, 1 month ago)

APE is a true albino, and thus has no pigment whatsoever: even the spores


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SunnyDayze]
    #25703736 - 12/26/18 12:06 PM (5 years, 1 month ago)

Idk... maybe


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens]
    #25703974 - 12/26/18 02:16 PM (5 years, 1 month ago)

I pour when I can hold the media bottle comfortably, bare handed... sometimes, the bottom has started to coagulate, and the last couple of dishes end up a little thick... stack them as high as I can in the SAB... usually only the top 2 plates of each stack have condensation, and very little, which clears up before I use them typically


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Duhrwood Stevens] * 1
    #25704062 - 12/26/18 03:02 PM (5 years, 1 month ago)

I'm talking comfortably... for normal folks: shower temp lol


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dabadoodledoo710]
    #25704090 - 12/26/18 03:17 PM (5 years, 1 month ago)

RW clone, T3:



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Re: AGAR ENVY! (Anything and All things agar!) [Re: MH5109]
    #25704118 - 12/26/18 03:33 PM (5 years, 1 month ago)

All stays well, prep more oats in a few days... got 14 qts RW, 4 qts PE6, and 2 quarts Texas going already... got the bug bad after thanksgiving/not cultivating anything but plates for like 6 weeks


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