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InvisibleJHOVA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #25662247 - 12/06/18 05:41 PM (5 years, 1 month ago)



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 1
    #25662560 - 12/06/18 07:56 PM (5 years, 1 month ago)

Quote:

Tormato said:
Quote:

JHOVA said:




Swab?



pray it germs. this shit hasnt the last 2 times i swiped it :crankey: its fuckin stuck in that bitch.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: JHOVA]
    #25668429 - 12/09/18 05:24 PM (5 years, 1 month ago)

Quote:

JHOVA said:








Nasty


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InvisibleJHOVA
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25672063 - 12/11/18 10:50 AM (5 years, 1 month ago)

Quote:

Tormato said:
Quote:

JHOVA said:
Quote:

JHOVA said:








Nasty



Dammit!

:fuckthisshit:




:lmafo: i got a fresh swab coming.:dogpipe:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mateja]
    #25847303 - 03/02/19 08:56 AM (4 years, 10 months ago)

.5% or 1.5%? Munchs recipe for Blenderless liquid inoculant is 1.7% and falls apart as it is very easily. 2 grams of agar per 120 mls. 2/120 = 1.666%:cookiemonster:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: topohunt]
    #25853065 - 03/04/19 08:55 PM (4 years, 10 months ago)

id transfer once more with the uniform growth on the right side of the second pic.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: KoalaJesus]
    #26041677 - 06/09/19 11:55 AM (4 years, 7 months ago)

Take pictures in a sab through the arm holes side ways


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Re: AGAR ENVY! (Anything and All things agar!) [Re: KoalaJesus]
    #26041883 - 06/09/19 02:10 PM (4 years, 7 months ago)

Make an inoculation loop and use a single drop of solution on the tip. Then spread it in a S shape. Kinda hard to do in a pasty plate. Id suggest larger containers like wide mouth half pints. When you spray a plate with solution directly the solution and contams will mesh and roll around the entire plate making identification harder.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: KoalaJesus]
    #26041994 - 06/09/19 03:04 PM (4 years, 7 months ago)

Quote:

KoalaJesus said:
Quote:

JHOVA said:
Make an inoculation loop and use a single drop of solution on the tip. Then spread it in a S shape. Kinda hard to do in a pasty plate. Id suggest larger containers like wide mouth half pints. When you spray a plate with solution directly the solution and contams will mesh and roll around the entire plate making identification harder.




Good stuff. I've come across others that have used that same method. I would need to sterilize both the loop and the syringe though, correct?

I have some jars to use, but I also have this metal tool that has a loop on the end, however its bent at an angle. Could work out well as something to use with higher edge containers.

I will say the identification is tough. Just need to keep at it and get more experience to be able to recognize the good/bad.




yes a loop and syringe are always flame sterilized before use and in between uses.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: footpath]
    #26976634 - 10/09/20 08:30 AM (3 years, 3 months ago)

A week to a week to a week and a half depending on transfer size


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Re: AGAR ENVY! (Anything and All things agar!) [Re: manlord]
    #27340941 - 06/08/21 11:49 PM (2 years, 7 months ago)

Make new plates. Theres nothing good there. Transfer early when you see something clean.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: manlord]
    #27341292 - 06/09/21 08:42 AM (2 years, 7 months ago)

Shoebox them individually or top fruit. Wouldnt spawn a mono.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: wes_terr2] * 3
    #28098997 - 12/15/22 03:36 PM (1 year, 1 month ago)

Quote:

wes_terr2 said:
Pre-sterilized from amazon. From a seller called "Evviva Sciences". It's MEA with charcoal.




Just so you know you could do "agar" with brf pucks in 1/2 pint jars with steam sterilization with no PC or buy lotkids agar plates heard his agar guarantees triple :yields:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet]
    #28099344 - 12/15/22 06:43 PM (1 year, 1 month ago)

Why would you ever hold the lid????? The lid is on the cookie rack upside down lol.

Are you legit leaving it right aide up on the cookie rack or what?

If multiple people are confused by the purpose of your post maybe its bad communication and theres lots of people using no pour successfully that dont have an inert top layer or contamination. You did mention being overly verbose in a previous post.....

Ur real defensive that its the tek and not the user but i dont think thats the case. If youd be less aggro people could help you out.:shrug:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 1
    #28099396 - 12/15/22 07:05 PM (1 year, 1 month ago)

You hold a no pour in your left hand and scalpel in the right. It should be side ways facing the holes of your sab. Nothing floats on top of it including your fingers that way.

Munchauzen has a video on how to do transfers where he demonstrates the technique.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28099416 - 12/15/22 07:10 PM (1 year, 1 month ago)

Howeva you like as long as the plate aint vertical


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rusty2096] * 1
    #28099463 - 12/15/22 07:29 PM (1 year, 1 month ago)

Quote:

Rusty2096 said:
But seriously, anyone ever considered (or did)  do SAB work with the plates upside down?

So you pick up the plate instead of the lid, take what you need and closet it back?






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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28102171 - 12/17/22 02:22 PM (1 year, 1 month ago)

You just need to see bubbles coming up and its good. Then stir it a couple times to make sure its mixed.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nonagon] * 1
    #28104292 - 12/18/22 09:50 PM (1 year, 1 month ago)

All natural food coloring from sprouts is not good for agar. The color fades when heated up. :crankey:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #28104767 - 12/19/22 12:03 PM (1 year, 1 month ago)

Is it the grain?


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