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InvisibleJim
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Registered: 04/07/04
Posts: 20,898
Reusing a spore syringe.
    #2565826 - 04/15/04 10:49 PM (13 years, 15 days ago)

Can I use a syringe in more than on use? I mean with the spores still in it. I want to innoculate three jars in the morning, but I only have a 20cc syringe. Would it be ok to just put some solution in the jars and the re cap the syringe and refridgerate till next use(assuming I will resterylize the needle when I do more jars)?

Help!


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Invisibledobinky
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Re: Reusing a spore syringe. [Re: Jim]
    #2565838 - 04/15/04 10:52 PM (13 years, 15 days ago)

yep!


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InvisiblePrisoner#1M
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Re: Reusing a spore syringe. [Re: Jim]
    #2565843 - 04/15/04 10:54 PM (13 years, 15 days ago)

you should shake it very well and use about 1/2-1cc of solution per jar... re cap the syringe and use it at a later time... make sure you sterilize the needle with a flame or alcohol between each jar and each use...


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InvisibleJim
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Re: Reusing a spore syringe. [Re: Prisoner#1]
    #2565853 - 04/15/04 10:57 PM (13 years, 15 days ago)

thanks guys! it wasnt too clear when I was researching the site!


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afoaf said:
Jim, if you were in my city, I would let you fuck my wife.


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OfflineJohnnyJ
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Registered: 04/14/04
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Re: Reusing a spore syringe. [Re: Jim]
    #2566110 - 04/16/04 12:33 AM (13 years, 15 days ago)

Is it necessary to keep the syringe in a fridge?
JohnnyJ


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OfflineDerek
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Re: Reusing a spore syringe. [Re: JohnnyJ]
    #2566124 - 04/16/04 12:39 AM (13 years, 15 days ago)

not absolutely necessary, but it helps them last longer.


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OfflineThatDaltonGuy
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Re: Reusing a spore syringe. [Re: Derek]
    #2566232 - 04/16/04 01:23 AM (13 years, 14 days ago)

cold temperatures slow down most all fungi growth... including the aging of them... and this includes the spores... fridge makes it last longer...

a spore print exposed to air usualy lasts around 3-5 months....
spores in water can rehydrated themselves and usual last alot longer... possibly 2 or 3 years... now imagine if u exposed them to cold temperatures like in the fridge? =) anyways happy shrooming


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InvisiblePrisoner#1M
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Re: Reusing a spore syringe. [Re: ThatDaltonGuy]
    #2566816 - 04/16/04 08:59 AM (13 years, 14 days ago)

incorrect... a spore print can last for more than 5 years and remain viable, a syrince's life expectancy is under a year (they both often last longer), under refridgeration

I allowed a friend to have several syringes that were sent to me, they were all over 2 years old and had been stored outside in a shed, all syringes were still viable even though they were exposed to temps that reached 95+ and below freezing over that 2 year period.

one of my first grows several years ago was with a spore print that was 7 years old, it was run on agar which rehydrated the spores and although not all the spores had germinated they were still quite viable


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Offlineblackout
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Re: Reusing a spore syringe. [Re: Prisoner#1]
    #2567280 - 04/16/04 12:21 PM (13 years, 14 days ago)

yep prints last longer.

you can always sterilise some water and suck it up into your half used syringe to "regenerate" it. especially if it is a nice dark one. if it is light give it a good shake each time.


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Offlinetrancefishy
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Re: Reusing a spore syringe. [Re: Jim]
    #2569458 - 04/16/04 10:49 PM (13 years, 14 days ago)

not only can you use it on several occasions, after you grow some, you can refill your syringes pretty easily with your own grown spores, becoming totally self sufficient (and cheap, since spores are easily the most expensive part of that hobby)


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