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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: Mycolorado]
#25649668 - 11/30/18 11:07 PM (5 years, 2 months ago) |
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Quote:
Mycolorado said: Boiling agar before pc’ing is retarded. 
Add dry ingredients and room temp tap to bottle. Swirl or stir if you have a clump of dry ingredients stuck to bottom. PC. Done.
DFA
  
If you're pcing your agar in two separate bottles as I sometimes do depending on how much I need, then I'll microwave because how do you know you are getting the same amount of nutrients/agar in both bottles if half of it is sat at the bottom of the measuring jug?
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 "great things may come to those who wait, but only what's left by those who hustle"
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Bumholio]
#25649677 - 11/30/18 11:09 PM (5 years, 2 months ago) |
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Do the math per bottle.
Mines 3.75 grams agar 7 grams lme per 400ml and that's per bottle.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Bumholio
What's the craic



Registered: 07/23/18
Posts: 4,269
Loc: Shroomsville
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Re: Cultivation General Discussion [Re: van hatton]
#25649796 - 12/01/18 01:44 AM (5 years, 2 months ago) |
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By the time you've weighed and measured everything twice mines is already finished in the microwave. So no real advantage to either way. Just personal preference I guess
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 "great things may come to those who wait, but only what's left by those who hustle"
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: Bumholio]
#25649818 - 12/01/18 02:52 AM (5 years, 2 months ago) |
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I want to make a big batch of LC now that I have clean agar plates to take wedges or biopsies from.
I tried grain water already but my order of dry malt extract just showed up and I wanna give it a go. It should be fine just with that and water right? I don't actually need dextrose?
What ratio? couple grams to a litre? or? And lastly, do you guys filter the LME broth so it doesn't have sediment in there or you just leave it ?
Thnks for your help.
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MH5109
Stranger


Registered: 09/27/17
Posts: 1,385
Loc: America
Last seen: 3 years, 25 days
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Re: Cultivation General Discussion *DELETED* [Re: sh4d0ws]
#25649835 - 12/01/18 03:26 AM (5 years, 2 months ago) |
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Post deleted by MH5109
Reason for deletion: Cant read.
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Edited by MH5109 (12/01/18 03:28 AM)
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sh4d0ws
LSx


Registered: 02/26/08
Posts: 12,086
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Re: Cultivation General Discussion [Re: MH5109]
#25649848 - 12/01/18 03:40 AM (5 years, 2 months ago) |
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I'm Not doing agar. Just LC. I imagine the same amount of LME is probably fine though
I'll just experiment, lol and whip up 4 or 5 see which one does best. Gonna try 8 grams to 500mL see how it looks. If it ends up too dark I'll dilute. Sediment, half I'll filter half I won't and see which ones do better.
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Shroomspective
Looking into the void



Registered: 09/26/15
Posts: 1,725
Loc: Far Far Away
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Re: Cultivation General Discussion [Re: sh4d0ws] 2
#25649861 - 12/01/18 04:03 AM (5 years, 2 months ago) |
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On the agar subject, if you read good cooking guides for Agar they always say to use Cold water to dissolve it, as it's insoluble when cold, but disperses correctly. Adding to hot water result in lumps.
https://loveisinmytummy.com/2016/05/all-you-need-to-know-about-agar-agar.html "If using agar powder, mix all the ingredients along with the agar and let it sit for 5 minutes. Never mix agar powder with warm/hot water as it will clump and become impossible to dissolve. Stir into room temperature liquid and then bring it to a rolling boil, making sure the agar has dissolved"
I only ever start with cold water and heat for a few mins as you get perfect consistency
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MH5109
Stranger


Registered: 09/27/17
Posts: 1,385
Loc: America
Last seen: 3 years, 25 days
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Re: Cultivation General Discussion [Re: sh4d0ws]
#25649870 - 12/01/18 04:16 AM (5 years, 2 months ago) |
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Quote:
sh4d0ws said: I'm Not doing agar. Just LC. I imagine the same amount of LME is probably fine though
I'll just experiment, lol and whip up 4 or 5 see which one does best. Gonna try 8 grams to 500mL see how it looks. If it ends up too dark I'll dilute. Sediment, half I'll filter half I won't and see which ones do better.
Misread the post, my fault m8, I know nothing about LC, other than they are a great inoculation method, and are a great way to fuck up a pile of grain, mostly my experience.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Cultivation General Discussion [Re: Mycolorado]
#25650148 - 12/01/18 08:29 AM (5 years, 2 months ago) |
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Quote:
Mycolorado said: Yeah, I hear ya...a mycologist taught me to make agar and pour plates in a mycology lab. We never boiled anything before pc’ing. If you want to boil shit, go for it. It’s still a waste of time. 
If it takes some time for the dry patches to dissolve during the cycle you're cutting your cycle time short. At only 15m at 15psi if your agar dissolves during that time you should add time.
When I worked in lab at University there was media that was heat sensitive so we cooked it at 20psi for 3 minutes rather than 15psi for 15m. For whatever reason hotter for less time was safer for the media to not break down. But with only a 3m cycle you need to make sure youre homogeneous before running the cycle.
And with malt extract upon the first time it reaches boiling for a few minutes a bunch of proteins precipitate out making your media hazy as a final product. Unless you decant it off the crud first
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Crispykoot
Jello Wrangler



Registered: 10/16/16
Posts: 5,922
Loc:
Last seen: 4 days, 3 hours
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Re: Cultivation General Discussion [Re: bodhisatta]
#25650327 - 12/01/18 10:03 AM (5 years, 2 months ago) |
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I use a FP pre mix right now (for a year or so) and I just weigh it, mix with hot tap water in my flask and then PC for 45 minutes at 15psi. Works great every time.
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Shadowboxing the apocalypse and wandering the land
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van hatton
Still a noob


Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: Crispykoot]
#25650338 - 12/01/18 10:08 AM (5 years, 2 months ago) |
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doesn't seem to effect my clarity at all
My PC hardly stays at 15 it's usually in the 18-20 range so I think I'll be okay I've been doing it for like 500-600 petris.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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Guy on a buffalo
Apprentice Cultivator


Registered: 10/30/18
Posts: 351
Loc: Ridin around through the ...
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Re: Cultivation General Discussion [Re: van hatton]
#25650416 - 12/01/18 10:59 AM (5 years, 2 months ago) |
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Ate 3.5g last night of my first harvest of GT...Meh. maybe grew some bunk shrooms 
1st time in 20 years so thought I'd get blasted. Hope future batches are better
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 1 month, 2 days
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Are these what I want? Slideable? https://www.amazon.com/gp/product/B014AFZ820/ref=ox_sc_act_title_1?smid=A3HAEZYY8XWH36&psc=1 Found them for just under $60 a case shipped,but don't want to get the wrong type.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 13 hours, 11 minutes
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Re: Cultivation General Discussion [Re: mushroomnate]
#25650473 - 12/01/18 11:32 AM (5 years, 2 months ago) |
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No clue what that means but they'll work.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Brain Bulb
Insane in the Membrane



Registered: 11/09/17
Posts: 1,358
Last seen: 7 months, 24 days
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Re: Cultivation General Discussion [Re: mushroomnate]
#25650698 - 12/01/18 01:31 PM (5 years, 2 months ago) |
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Quote:
mushroomnate said: Are these what I want? Slideable? https://www.amazon.com/gp/product/B014AFZ820/ref=ox_sc_act_title_1?smid=A3HAEZYY8XWH36&psc=1 Found them for just under $60 a case shipped,but don't want to get the wrong type.
Mycosupply.com has 500 for $99. A little more $$ but should be more reliable.
https://mycosupply.com/product/disposable-plastic-petri-dishes/
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 1 month, 2 days
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Re: Cultivation General Discussion [Re: Brain Bulb]
#25650710 - 12/01/18 01:37 PM (5 years, 2 months ago) |
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I've read good things about the celltreat,but I don't know what "slideable" means,and if it is something I do not want for this purpose.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 13 hours, 11 minutes
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Re: Cultivation General Discussion [Re: mushroomnate]
#25650788 - 12/01/18 02:25 PM (5 years, 2 months ago) |
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I dont think it matters much. Some dishes have rings that lock them in place so stacks dont topple. My guess is these are the ones without
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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mushroomnate
Pstranger



Registered: 05/17/17
Posts: 3,100
Last seen: 1 month, 2 days
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Re: Cultivation General Discussion [Re: tryptkaloids]
#25650838 - 12/01/18 02:53 PM (5 years, 2 months ago) |
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 Thanks!
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Dabadoodledoo710
Dab Connoisseur



Registered: 03/04/17
Posts: 1,140
Loc: Zone 5b
Last seen: 1 month, 23 days
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Re: Cultivation General Discussion [Re: mushroomnate]
#25650906 - 12/01/18 03:22 PM (5 years, 2 months ago) |
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I've used the gripring and slideable plates by celltreat they both work great
Edited by Dabadoodledoo710 (12/01/18 03:22 PM)
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Psicomb
shroom or die



Registered: 01/13/18
Posts: 4,716
Loc: the womb
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Is it normal for PE to take over 2 weeks to show knotting/pinning? I don't have knots yet in my tubs and it has been 14 days. The growth looks very healthy in all the tubs, though, and they have been fully colonized for almost a week. I have done APE before successfully but not PE so I am used to seeing pins within 10-ish days.
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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something - nick sand
Edited by Psicomb (12/01/18 04:13 PM)
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