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OfflineWesleyStipes
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Registered: 10/15/18
Posts: 105
Last seen: 6 years, 1 month
No growth or contam after ms
    #25589458 - 11/03/18 04:09 PM (6 years, 2 months ago)



Three jars contaminated the rest show no growth. Any ideas for the spent substrate besides tossing? Crystals if the gods maybe idk? Hope and innovation is what I seek

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OfflineGele
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Registered: 11/12/17
Posts: 166
Loc: The planet Earth
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Re: No growth or contam after ms [Re: WesleyStipes]
    #25589611 - 11/03/18 05:07 PM (6 years, 2 months ago)

Quote:

WesleyStipes said:


Three jars contaminated the rest show no growth. Any ideas for the spent substrate besides tossing? Crystals if the gods maybe idk? Hope and innovation is what I seek



NO HOPE


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OfflineSolipsis
m̶a̶d̶ disappointed scientist
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Registered: 12/28/09
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Re: No growth or contam after ms [Re: Gele]
    #25589687 - 11/03/18 05:41 PM (6 years, 2 months ago)

It's not really spent substrate like myc thats done fruiting or whatever. You can't extract if there is no clean myc, or any myc really.

As for 'invisible' contams: if you are not just dealing with stalling it is probably that you are not experienced enough yet with a certain contam to recognize it or (/and, this is kind of an example:) yeast infections e.g. can look like there is just nothing at all going on in there. However you should be able to smell it.

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OfflineVroomerMcZoomers
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Re: No growth or contam after ms [Re: WesleyStipes]
    #25589711 - 11/03/18 05:51 PM (6 years, 2 months ago)

What's in those jars?


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Either you repeat the same conventional doctrines everybody is saying, or else you say something true, and it will sound like it's from Neptune.  -Noam Chomsky

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OfflineCzM8
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Registered: 05/25/18
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Re: No growth or contam after ms [Re: WesleyStipes]
    #25589731 - 11/03/18 06:03 PM (6 years, 2 months ago)

Hope is to start again. Innovation is to either purchase from another supplier AND/or revisit your tek with new spores.

I've had jars just not take for whatever reason. I kept them on my shelf for weeks, and re-shot them with new spores and it actually worked for me. Not a suggested method, rather a reassurance that it could just be the spores?


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Edited by CzM8 (11/03/18 06:04 PM)

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InvisibleLt Cmdr La Forge
I'm a teapot

Registered: 10/15/18
Posts: 43
Loc: Canada Flag
Re: No growth or contam after ms [Re: CzM8]
    #25590174 - 11/03/18 09:09 PM (6 years, 2 months ago)

most certainly my man, i've purchased 'professionally done' syringes with nice sticker labels, strain info, time and date made, etc and had them colonize like lazy bastards.

i'd go with another vendor and just make sure you have more than enough, if they're done well enough you can get away with blasting 2mL in each and watching them take off in under a week.


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The fabric of space is a matrix of information encoded in geometric coordinates. The biological being crystallize from this field and is able to connect multiple space-time coordinates by means non-local wormhole entanglement mediated through the resonance of microtubule networks. We are made of, and woven into, the fabric of space to such a degree that the spacememory field is an integral part of our anatomy.

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OfflineWesleyStipes
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Registered: 10/15/18
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Re: No growth or contam after ms [Re: Lt Cmdr La Forge]
    #25592917 - 11/05/18 01:03 AM (6 years, 2 months ago)

I think that may have been the issue I only put .25-.5 in each jar. Without agar I guess that was more than hopeful

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OfflinePiaseski
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Registered: 11/09/15
Posts: 391
Last seen: 1 year, 1 month
Re: No growth or contam after ms [Re: WesleyStipes]
    #25593078 - 11/05/18 04:09 AM (6 years, 2 months ago)

Your jar appears to be too full imo.


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OfflineWesleyStipes
Stranger

Registered: 10/15/18
Posts: 105
Last seen: 6 years, 1 month
Re: No growth or contam after ms [Re: Piaseski]
    #25631838 - 11/22/18 08:16 AM (6 years, 1 month ago)

I made another attempt with your advice in mind. What do you think of these?
https://www.shroomery.org/forums/showflat.php/Number/25631518

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