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OfflineNEOTYKE
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Pf tek jars not showing signs.
    #25628686 - 11/20/18 08:15 PM (6 years, 1 month ago)

Inoculated 4 jars on the 14th. Todays the 20th and they are not showing signs of growth. It is my own pf tek and I followed the 2-1-1 recipe. I inoculated with rubber syringe port and have micropore tape. Thanks.

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OfflineInfinitysquared
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Re: Pf tek jars not showing signs. [Re: NEOTYKE]
    #25628703 - 11/20/18 08:28 PM (6 years, 1 month ago)

Give it time...

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OfflineRot
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Re: Pf tek jars not showing signs. [Re: Infinitysquared]
    #25628705 - 11/20/18 08:31 PM (6 years, 1 month ago)

Just did my first set of jars and 2nd set a few days ago. I saw signs of growth on day 3 for both batches.


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OfflineTormatoMFacebookDiscordReddit
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Re: Pf tek jars not showing signs. [Re: Infinitysquared]
    #25628707 - 11/20/18 08:32 PM (6 years, 1 month ago)

Quote:

Infinitysquared said:
Give it time...



:whathesaid: Sometimes it can take a couple of weeks bud....B Patient. :cool:


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Invisiblevan hattonFacebookDiscord
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Re: Pf tek jars not showing signs. [Re: Infinitysquared]
    #25628708 - 11/20/18 08:32 PM (6 years, 1 month ago)

Quote:

Infinitysquared said:
Give it time...




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OfflineNEOTYKE
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Re: Pf tek jars not showing signs. [Re: van hatton]
    #25628752 - 11/20/18 08:59 PM (6 years, 1 month ago)

I have another question for you. I inoculated 2 more jars right out of PC. I made sure to go through sterile procedure. I completely got mind blocked and was to eager and realized I forgot to let cool until after inoculation. The were still on the hotter side. I could touch jars. Will the hotter temp kill the spores or am I okay?

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Re: Pf tek jars not showing signs. [Re: NEOTYKE]
    #25628757 - 11/20/18 09:01 PM (6 years, 1 month ago)

Likely dead af


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OfflineTormatoMFacebookDiscordReddit
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Re: Pf tek jars not showing signs. [Re: cronicr]
    #25629257 - 11/21/18 05:51 AM (6 years, 1 month ago)

Quote:

cronicr said:
Likely dead af



:whathesaid:
:youcaughtme:


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InvisibleMateja
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Re: Pf tek jars not showing signs. [Re: Tormato]
    #25629313 - 11/21/18 06:49 AM (6 years, 1 month ago)

So how sensitive are spores to heat? At what temp do spores start to die and for how long period?

I'm thinking those jars did not stay warm for especially long time outside the PC, if they were cool enough to touch and handle then I would assume that they were back to at least 30-35C in like 30 min (assuming jars were about 45-50C when inoculated but then again I don't think he would have inoculated the jars if they were as hot as an agar bottle or hotter. That's almost uncomfortable to hold in your hand :shrug:


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OfflineTormatoMFacebookDiscordReddit
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Re: Pf tek jars not showing signs. [Re: Mateja]
    #25629338 - 11/21/18 07:08 AM (6 years, 1 month ago)

Honestly, I have done this a few times (high af) and the jars never germinated. I assumed it was due to temps, BUT you do bring up a good point Mateah...I never really bothered to research that. :shrug:


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InvisibleNimph
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Re: Pf tek jars not showing signs. [Re: NEOTYKE]
    #25629417 - 11/21/18 08:01 AM (6 years, 1 month ago)

2-1-1??


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OfflineTormatoMFacebookDiscordReddit
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Re: Pf tek jars not showing signs. [Re: Nimph]
    #25629468 - 11/21/18 08:37 AM (6 years, 1 month ago)

Quote:

Nimph said:
2-1-1??



I'm assuming Verm:Water:BRF


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OfflineBloomingAlchemy
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Re: Pf tek jars not showing signs. [Re: Tormato]
    #25629652 - 11/21/18 10:17 AM (6 years, 1 month ago)

All you can do is wait a few more days and see. Your temperatures can dictate how quickly you see growth too. If it's a little cooler where you are, then give it another week before you start to worry. As for the ones you knocked up while hot, they might be ok if the jars had really cooled down enough to handle. But IME when I have overheated spores too much, my jars did not germinate.


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InvisibleMateja
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Re: Pf tek jars not showing signs. [Re: Tormato]
    #25629883 - 11/21/18 12:29 PM (6 years, 1 month ago)

https://www.shroomery.org/forums/showflat.php/Number/3680821#3680821 only found this 13 year old thread.

Quote:

hyphae said:
106F. will kill myc not spores. Thermo death limits for cubie spores are around 50C. (122F.)




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OfflineNEOTYKE
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Re: Pf tek jars not showing signs. [Re: Mateja]
    #25630323 - 11/21/18 03:14 PM (6 years, 1 month ago)

Thanks for all the help guys. Im just gonna see what they do since that all I can do. One of my 4 jars is showing signs now. Also has some purple mold growing? Clearly contaminated. Question is now, with what?

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Re: Pf tek jars not showing signs. [Re: NEOTYKE]
    #25630330 - 11/21/18 03:16 PM (6 years, 1 month ago)

Lipstick mold


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Offlinedelcat
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Re: Pf tek jars not showing signs. [Re: cronicr]
    #25630389 - 11/21/18 03:44 PM (6 years, 1 month ago)

Who cares what the contam is? You gotta throw it out.

PF Tek, and grains, can take 2-3 weeks to show signs of anything.  I've had jars before Ive shaken after 3 weeks of seeing no growth to find a mass of mycelium in the middle out of view!

With PF Tek just follow the tek to the letter, make sure to let it cool, inoculate the side of the jars (point needle at the glass) and you should be fine. You'll be able to see right away when the spores germinate and mycelium develops.

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