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Offlineominousmike

Registered: 10/24/18
Posts: 28
Last seen: 1 year, 6 months
Help with first agar transfer
    #25625440 - 11/19/18 01:57 PM (6 years, 1 month ago)

I've recently gotten back into cultivation after a 12 year break. Back then it was glove boxes, I don't remember ever seeing anything about a SAB. Messing with agar seemed undoable without a flow hood back then. Everything I did was just straight spore syringes or prints to grain jars.

Now that I'm giving it a go again, I'm just trying to throw myself into everything so I can learn as much as possible. I've got PF cakes going. Grain jars inoculated with spore syringes. I've basically divided Bods flowchart into 3 branches (not doing "inoculate LC" branch) and I'm trying to experience everything.

Over the past few weeks I've inoculated 6 separate sets of agar plates (4 petri 2 pastywhyte) in the SAB. Below I've listed the first 3 sets that I did, these are the ones showing growth.

Blue -  11/3  MEA - Treasure Coast
Green -  11/4  MEA - Treasure Coast
Red -  11/7  MEA with yeast - Puerto Rican







The green ones are mostly fully colonized across the entire container. I figure its time to transfer them to new plates. I've got a few questions.

There doesn't seem to be to be an ideal place to cut out. 1) Should I just be cutting little squares of mycelium and placing them in the center of newly poured plates? 2) Am I doing this to start narrowing down the amount of colonies that the mycelium is made up of? 3) Since none of the plates appear to have contaminants I'm trying to get away from, and I just trying to transfer some clean mycelium on the first transfer?

I'm also hoping to get some feed back on my dishes in general. Does it appear that any of the other plates (red or blue) are ready to transfer to new Petri dishes as well, or should I be waiting for them to cover more of the plate.

Thank you in advance to anyone that helps!

W

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